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The map label for this gene is mutT [H]

Identifier: 83720449

GI number: 83720449

Start: 1277991

End: 1278440

Strand: Direct

Name: mutT [H]

Synonym: BTH_I1130

Alternate gene names: 83720449

Gene position: 1277991-1278440 (Clockwise)

Preceding gene: 83718824

Following gene: 83718947

Centisome position: 33.55

GC content: 68.44

Gene sequence:

>450_bases
ATGAGTATCGAAGTCGCGAAAGCGGCGGCGCCCGCCGACGCGGGCCGCAAGGTCACCGAGGTGGCGGTCGGAGTGCTGGT
GCAGCCGGACGGCCGATATTTGCTCGCGCAGCGGCTGCCCGGCAAGCCTTACGAGGGCTACTGGGAATTTCCGGGCGGCA
AGCTCGAGGCGGGCGAGAGTGTCGAGGAGGCGCTTGCCCGGGAACTGCACGAGGAACTGGGCATCGACGTGACCGAATGC
CATCGCTGGCATACGCTCGAGCACGACTATCCGCACGCGTACGTTCGCCTGTACTTCTGCAAGGTGACGGGCTGGACGGG
CGAGCCGCACAGCCGCGAAGGCCAGGCGTTCGTCTGGCAGCATCTGCCCGTTGACGTCGCTCCGCTGCTGCCCGCCGCGC
TGCCCGTGCTCGATCTGCTCGCGCGCGAGCAGGGGGCGGCGCAGCGATAA

Upstream 100 bases:

>100_bases
CGCGCGGCGACGCGCTCGTCTGGGCGCTCGAGCGCGGCTCGCGCTCCGGCCGGGTCGCATGGCAGTTCGCGCGCGACCGC
GCGGGCCGCAAGGAGAACGT

Downstream 100 bases:

>100_bases
ACATGCGCGGAGCCTTCGGCTCCGCAGCCGTTCATCCAGTCTTATCGGGCCGATGATCGGCTGCGCTCCCCCGTGAGCGG
CTCGCTGCCTGACCGCACTC

Product: NUDIX family pyrophosphatase

Products: 8-Oxo-Dgmp; Pyrophosphate. [C]

Alternate protein names: 7,8-dihydro-8-oxoguanine-triphosphatase; 8-oxo-dGTPase; dGTP pyrophosphohydrolase [H]

Number of amino acids: Translated: 149; Mature: 148

Protein sequence:

>149_residues
MSIEVAKAAAPADAGRKVTEVAVGVLVQPDGRYLLAQRLPGKPYEGYWEFPGGKLEAGESVEEALARELHEELGIDVTEC
HRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSREGQAFVWQHLPVDVAPLLPAALPVLDLLAREQGAAQR

Sequences:

>Translated_149_residues
MSIEVAKAAAPADAGRKVTEVAVGVLVQPDGRYLLAQRLPGKPYEGYWEFPGGKLEAGESVEEALARELHEELGIDVTEC
HRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSREGQAFVWQHLPVDVAPLLPAALPVLDLLAREQGAAQR
>Mature_148_residues
SIEVAKAAAPADAGRKVTEVAVGVLVQPDGRYLLAQRLPGKPYEGYWEFPGGKLEAGESVEEALARELHEELGIDVTECH
RWHTLEHDYPHAYVRLYFCKVTGWTGEPHSREGQAFVWQHLPVDVAPLLPAALPVLDLLAREQGAAQR

Specific function: Involved in the GO system responsible for removing an oxidatively damaged form of guanine (7,8-dihydro-8-oxoguanine) from DNA and the nucleotide pool. 8-oxo-dGTP is inserted opposite dA and dC residues of template DNA with almost equal efficiency thus lea

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1786288, Length=127, Percent_Identity=34.6456692913386, Blast_Score=79, Evalue=1e-16,
Organism=Escherichia coli, GI1788056, Length=124, Percent_Identity=37.9032258064516, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003561
- InterPro:   IPR020476
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: 3.6.1.- [C]

Molecular weight: Translated: 16518; Mature: 16387

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEVAKAAAPADAGRKVTEVAVGVLVQPDGRYLLAQRLPGKPYEGYWEFPGGKLEAGES
CCCCCCCCCCCCCCCCHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCH
VEEALARELHEELGIDVTECHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSREGQAFVWQ
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEE
HLPVDVAPLLPAALPVLDLLAREQGAAQR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure 
SIEVAKAAAPADAGRKVTEVAVGVLVQPDGRYLLAQRLPGKPYEGYWEFPGGKLEAGES
CCCCCCCCCCCCCCCHHHHHHEEEEECCCCCEEEEECCCCCCCCCEEECCCCCCCCCCH
VEEALARELHEELGIDVTECHRWHTLEHDYPHAYVRLYFCKVTGWTGEPHSREGQAFVWQ
HHHHHHHHHHHHHCCCHHHHHHHCCCCCCCCCEEEEEEEEEEECCCCCCCCCCCCEEEEE
HLPVDVAPLLPAALPVLDLLAREQGAAQR
CCCCCHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: 8-Oxo-Dgtp; H2O [C]

Specific reaction: 8-Oxo-Dgtp + H2O = 8-Oxo-Dgmp + Pyrophosphate. [C]

General reaction: Hydrolase; Acting on acid anhydrides; In phosphorus-containing anhydrides [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]