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The map label for this gene is greB [H]

Identifier: 83720256

GI number: 83720256

Start: 1789688

End: 1790251

Strand: Direct

Name: greB [H]

Synonym: BTH_I1591

Alternate gene names: 83720256

Gene position: 1789688-1790251 (Clockwise)

Preceding gene: 83719914

Following gene: 83720914

Centisome position: 46.98

GC content: 64.54

Gene sequence:

>564_bases
ATGAACAAGGCGTTTGTGAAGGAGTCGGACGGAGACGACGACGACCTCGAACACGCGCAGGCGGCGATTCCGCCCGGCGC
GAAGAACTACATCACGCCGGCGGGCCACAAGCGCCTGCGCGACGAGTTGCTGCATCTGATCGACGAAGCGCGTCCCGAAG
TGGTGAAACTCGTGTCGTGGGCCGCGTCGAACGGCGATCGCTCGGAGAACGGCGACTACATCTATGGGAAGCGGCGTCTG
CGCGAAATCGATCGGCGCATCCGTTTTCTGACGAAGCGTCTCGACCTTGCCGAAGTCGTCGATGCAAGCCGCCAGGAGAA
CGCGGACCAGGTGTTCTTCGGGGCGACCGTCGATTACGCGACGCAGGACGGCATCGAGCACACGGTGACGATCGTCGGCA
TCGACGAGGTCGATCTCGATCGCGGGCATGTAAGCTGGATCTCGCCCATCGCGCGTGCGCTGCTGAAGGCGCGCGTCGGC
GACACGGTCACGCTGCTGACGCCTGTCGGTCCTGAGCCGATAGACGTGCTCGACGTCCGCTATCCGTCGCCGGGCGCGGC
TTGA

Upstream 100 bases:

>100_bases
TATAGCAAACAGATTTAAAAAGGGAAGCCCTTCTTGACGATTTATCGGACGATGCCGTTCGTGTAACCTTGGAGCATCCG
ATAGTCGTTGAAATTGCATC

Downstream 100 bases:

>100_bases
ACCGAGGCGTATCGGCGCGTTTTGCCGTCGTCGCGTCCTGCGGCGCACGAATTCGAACGGCGCGTATGACCGAATAAGGT
CGCGAATGCGCAGTCGGCCG

Product: transcription elongation factor GreB

Products: NA

Alternate protein names: Transcript cleavage factor greB [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MNKAFVKESDGDDDDLEHAQAAIPPGAKNYITPAGHKRLRDELLHLIDEARPEVVKLVSWAASNGDRSENGDYIYGKRRL
REIDRRIRFLTKRLDLAEVVDASRQENADQVFFGATVDYATQDGIEHTVTIVGIDEVDLDRGHVSWISPIARALLKARVG
DTVTLLTPVGPEPIDVLDVRYPSPGAA

Sequences:

>Translated_187_residues
MNKAFVKESDGDDDDLEHAQAAIPPGAKNYITPAGHKRLRDELLHLIDEARPEVVKLVSWAASNGDRSENGDYIYGKRRL
REIDRRIRFLTKRLDLAEVVDASRQENADQVFFGATVDYATQDGIEHTVTIVGIDEVDLDRGHVSWISPIARALLKARVG
DTVTLLTPVGPEPIDVLDVRYPSPGAA
>Mature_187_residues
MNKAFVKESDGDDDDLEHAQAAIPPGAKNYITPAGHKRLRDELLHLIDEARPEVVKLVSWAASNGDRSENGDYIYGKRRL
REIDRRIRFLTKRLDLAEVVDASRQENADQVFFGATVDYATQDGIEHTVTIVGIDEVDLDRGHVSWISPIARALLKARVG
DTVTLLTPVGPEPIDVLDVRYPSPGAA

Specific function: Necessary for efficient RNA polymerase transcription elongation past template-encoded arresting sites. The arresting sites in DNA have the property of trapping a certain fraction of elongating RNA polymerases that pass through, resulting in locked ternary

COG id: COG0782

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the greA/greB family [H]

Homologues:

Organism=Escherichia coli, GI87082261, Length=154, Percent_Identity=49.3506493506493, Blast_Score=142, Evalue=1e-35,
Organism=Escherichia coli, GI87082225, Length=152, Percent_Identity=34.8684210526316, Blast_Score=84, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018151
- InterPro:   IPR001437
- InterPro:   IPR022691
- InterPro:   IPR006358 [H]

Pfam domain/function: PF01272 GreA_GreB; PF03449 GreA_GreB_N [H]

EC number: NA

Molecular weight: Translated: 20709; Mature: 20709

Theoretical pI: Translated: 4.83; Mature: 4.83

Prosite motif: PS00829 GREAB_1 ; PS00830 GREAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
0.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKAFVKESDGDDDDLEHAQAAIPPGAKNYITPAGHKRLRDELLHLIDEARPEVVKLVSW
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
AASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDLAEVVDASRQENADQVFFGATVDYA
HHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCEECCH
TQDGIEHTVTIVGIDEVDLDRGHVSWISPIARALLKARVGDTVTLLTPVGPEPIDVLDVR
HHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEE
YPSPGAA
CCCCCCC
>Mature Secondary Structure
MNKAFVKESDGDDDDLEHAQAAIPPGAKNYITPAGHKRLRDELLHLIDEARPEVVKLVSW
CCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHH
AASNGDRSENGDYIYGKRRLREIDRRIRFLTKRLDLAEVVDASRQENADQVFFGATVDYA
HHCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCEEEECCEECCH
TQDGIEHTVTIVGIDEVDLDRGHVSWISPIARALLKARVGDTVTLLTPVGPEPIDVLDVR
HHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCEEEEEE
YPSPGAA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10761919 [H]