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The map label for this gene is murF [H]

Identifier: 83719896

GI number: 83719896

Start: 1257464

End: 1258870

Strand: Direct

Name: murF [H]

Synonym: BTH_I1114

Alternate gene names: 83719896

Gene position: 1257464-1258870 (Clockwise)

Preceding gene: 83720483

Following gene: 83719046

Centisome position: 33.01

GC content: 72.64

Gene sequence:

>1407_bases
ATGACGATGCTGACCTTGCGCGAAGCCGCAGCGATGATCCCCGGCGCGACCGTGCTCGGCGACGAGCGCGCGTCGTTCGA
GCGGGTGTCGACGGACAGCCGCTCGGCGGGGCCGGGCGATCTGTTCGTCGCGCTGAAGGGGGAGCGTTTCGATGCGCACG
ACTTCGTCGGCGACGTCGCCGCGCGCGGCGCGAGCGCCGCGATCGTGTCGCGCAGCACGGGCGAATGGAATCTGCCCGCG
CTGAAGGTGGCCGATACGCGCGCCGCGCTCGGCGCGCTCGCGCACGGCTGGCGGATGCGCTTTTCGATGCCGCTCGTCGC
GGTGACGGGCAGCAACGGCAAGACGACCGTGAAGGAGATGATCGCGTCGATTTTCGCCGCGGCGGTCGGCGTGCGCGCGC
GTCTTGCCACCGCAGGCAATTTCAACAACGACGTCGGCCTGCCGCTCACGCTGCTGCGCTTGAACGCCGCGCATCGGCTC
GCGGTCGTCGAGCTTGGAATGAACCACCCGGGCGAGACCGACGCGCTCGCGAAAATCGCCGCGCCGACGGTCGCGCTCGT
CAACAACGCGCAGCGCGAGCATCAGGAATTCATGGCGACGGTCGAGGCGGTCGCGCTCGAGCACGCGGCCGTCATCCATG
CACTCGGCCCGGACGGCGTCGCGGTGTTTCCGGCCGATGACGCGTATGCGGGCATCTGGCGCGTCGCGGCGACCGGCAAC
CGGATCGTCGATTTCGCGCTGCACGGGCTCGGGCGCGACACGCCCGCCGCGGTGACGGGCGTGCTCGACGGCAGCCGGCT
CGCGATCGACACGCCCGACGGCCGCGTCGACGTGCGCCTCTATGCGCTCGGCGAGCACAACGCGCGCAACGCGCTCGCCG
CGACCGCGGCGGCGCTCGCGGCCGGCGTGCCGCTCGCGGCGGTCAAGGCGGGGCTCGAGGCGTTCGCGCCGGTGAAGGGG
CGGCTGCAGGTGACGCACGCGGCGCTCGGTTCGCTCGCGGGCGCGACGGTGATCGACGACACCTACAACGCGAACCCCGA
TTCGATGCGCGCCGCGATCGACGTGCTCGCCGCGTGCTCGGCGCCGCGCGTGCTCGTGATCGGCGAGATGGGCGAGGTCG
GCGACGAAGGGCCGGCGTTCCACCGCGAGATCGGCGCGTATGCGCGCGAGCGCGGGATCGATGCGCTCTTCGCGCTCGGC
GCGGCGACGCTGCCCGCGTGCGAGGCGTACGGCGCGTCCGCGCGGCATTTCGACGACGCGGCGGCGCTGGTCGCCGCGCT
CGTCGCCGAGCACTTCGGCGCACACGCGACGCTGCTCGTGAAGGGCTCGCGGTTCATGAAGATGGAGCGCGTGGTCGACG
CGCTCGCGAACGAAACCGCGTCGGGCGCAGCGCCCGGCGCACACTGA

Upstream 100 bases:

>100_bases
CGACGCAGGAGATCATGGGCAAGAAGCGTGCGTTCTCCGATCAGGATCACGCGCGCCTCGCGCTCGCCGCGCGCACGACG
CAGACTCGCGGAGGCGGCGA

Downstream 100 bases:

>100_bases
CGAAGGAAGTAAGCATGCTGCTGGCGTTGGCGCAATGGCTGCAAGGTGACGCAAGCTTTTTGCGCTTGTTCACGTACCTG
ACGTTCCGGGCGGTGATGGC

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTMLTLREAAAMIPGATVLGDERASFERVSTDSRSAGPGDLFVALKGERFDAHDFVGDVAARGASAAIVSRSTGEWNLPA
LKVADTRAALGALAHGWRMRFSMPLVAVTGSNGKTTVKEMIASIFAAAVGVRARLATAGNFNNDVGLPLTLLRLNAAHRL
AVVELGMNHPGETDALAKIAAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALGPDGVAVFPADDAYAGIWRVAATGN
RIVDFALHGLGRDTPAAVTGVLDGSRLAIDTPDGRVDVRLYALGEHNARNALAATAAALAAGVPLAAVKAGLEAFAPVKG
RLQVTHAALGSLAGATVIDDTYNANPDSMRAAIDVLAACSAPRVLVIGEMGEVGDEGPAFHREIGAYARERGIDALFALG
AATLPACEAYGASARHFDDAAALVAALVAEHFGAHATLLVKGSRFMKMERVVDALANETASGAAPGAH

Sequences:

>Translated_468_residues
MTMLTLREAAAMIPGATVLGDERASFERVSTDSRSAGPGDLFVALKGERFDAHDFVGDVAARGASAAIVSRSTGEWNLPA
LKVADTRAALGALAHGWRMRFSMPLVAVTGSNGKTTVKEMIASIFAAAVGVRARLATAGNFNNDVGLPLTLLRLNAAHRL
AVVELGMNHPGETDALAKIAAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALGPDGVAVFPADDAYAGIWRVAATGN
RIVDFALHGLGRDTPAAVTGVLDGSRLAIDTPDGRVDVRLYALGEHNARNALAATAAALAAGVPLAAVKAGLEAFAPVKG
RLQVTHAALGSLAGATVIDDTYNANPDSMRAAIDVLAACSAPRVLVIGEMGEVGDEGPAFHREIGAYARERGIDALFALG
AATLPACEAYGASARHFDDAAALVAALVAEHFGAHATLLVKGSRFMKMERVVDALANETASGAAPGAH
>Mature_467_residues
TMLTLREAAAMIPGATVLGDERASFERVSTDSRSAGPGDLFVALKGERFDAHDFVGDVAARGASAAIVSRSTGEWNLPAL
KVADTRAALGALAHGWRMRFSMPLVAVTGSNGKTTVKEMIASIFAAAVGVRARLATAGNFNNDVGLPLTLLRLNAAHRLA
VVELGMNHPGETDALAKIAAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALGPDGVAVFPADDAYAGIWRVAATGNR
IVDFALHGLGRDTPAAVTGVLDGSRLAIDTPDGRVDVRLYALGEHNARNALAATAAALAAGVPLAAVKAGLEAFAPVKGR
LQVTHAALGSLAGATVIDDTYNANPDSMRAAIDVLAACSAPRVLVIGEMGEVGDEGPAFHREIGAYARERGIDALFALGA
ATLPACEAYGASARHFDDAAALVAALVAEHFGAHATLLVKGSRFMKMERVVDALANETASGAAPGAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=459, Percent_Identity=40.5228758169935, Blast_Score=254, Evalue=1e-68,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48110; Mature: 47979

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLTLREAAAMIPGATVLGDERASFERVSTDSRSAGPGDLFVALKGERFDAHDFVGDVA
CCEEEHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGASAAIVSRSTGEWNLPALKVADTRAALGALAHGWRMRFSMPLVAVTGSNGKTTVKEM
HCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCEEEEECCEEEEECCCCCHHHHHH
IASIFAAAVGVRARLATAGNFNNDVGLPLTLLRLNAAHRLAVVELGMNHPGETDALAKIA
HHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEECCCHHEEEEEEECCCCCCCHHHHHHHH
APTVALVNNAQREHQEFMATVEAVALEHAAVIHALGPDGVAVFPADDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCEEEEECCCC
RIVDFALHGLGRDTPAAVTGVLDGSRLAIDTPDGRVDVRLYALGEHNARNALAATAAALA
HHHHHHHHCCCCCCCHHEEEEECCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHH
AGVPLAAVKAGLEAFAPVKGRLQVTHAALGSLAGATVIDDTYNANPDSMRAAIDVLAACS
HCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCC
APRVLVIGEMGEVGDEGPAFHREIGAYARERGIDALFALGAATLPACEAYGASARHFDDA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCCCHHHHH
AALVAALVAEHFGAHATLLVKGSRFMKMERVVDALANETASGAAPGAH
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TMLTLREAAAMIPGATVLGDERASFERVSTDSRSAGPGDLFVALKGERFDAHDFVGDVA
CEEEHHHHHHHCCCCEEECCCCHHHHHHCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGASAAIVSRSTGEWNLPALKVADTRAALGALAHGWRMRFSMPLVAVTGSNGKTTVKEM
HCCCCEEEEECCCCCCCCCEEEECHHHHHHHHHHHCCEEEEECCEEEEECCCCCHHHHHH
IASIFAAAVGVRARLATAGNFNNDVGLPLTLLRLNAAHRLAVVELGMNHPGETDALAKIA
HHHHHHHHHHHHHEEEECCCCCCCCCCCEEEEECCCHHEEEEEEECCCCCCCHHHHHHHH
APTVALVNNAQREHQEFMATVEAVALEHAAVIHALGPDGVAVFPADDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCEEEECCCCCCCCEEEEECCCC
RIVDFALHGLGRDTPAAVTGVLDGSRLAIDTPDGRVDVRLYALGEHNARNALAATAAALA
HHHHHHHHCCCCCCCHHEEEEECCCEEEEECCCCEEEEEEEEECCCCCHHHHHHHHHHHH
AGVPLAAVKAGLEAFAPVKGRLQVTHAALGSLAGATVIDDTYNANPDSMRAAIDVLAACS
HCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHCC
APRVLVIGEMGEVGDEGPAFHREIGAYARERGIDALFALGAATLPACEAYGASARHFDDA
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHCCHHHHCCCCCCHHHHH
AALVAALVAEHFGAHATLLVKGSRFMKMERVVDALANETASGAAPGAH
HHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]