Click here to switch to the map view.

The map label for this gene is mpl [H]

Identifier: 83719762

GI number: 83719762

Start: 1312675

End: 1314087

Strand: Direct

Name: mpl [H]

Synonym: BTH_I1168

Alternate gene names: 83719762

Gene position: 1312675-1314087 (Clockwise)

Preceding gene: 83719496

Following gene: 83719051

Centisome position: 34.46

GC content: 66.74

Gene sequence:

>1413_bases
ATGCATATCCACATTCTCGGCATCTGCGGCACCTTCATGGGTGGTCTGGCCGTGCTCGCCCGCGCGGCGGGCCATACGGT
GACCGGTTGCGACGCAGGCGTCTATCCGCCGATGAGCACGCAGCTCGAGGCGCAGGGCATCCGGCTGATCGAGGGCTACG
GCGCGGAACAGATCGACCTGAAGCCCGATCTGTTCGTGATCGGCAACGTGGTGTCGCGCGGCAACCCGCTGATGGAGGCG
ATCCTCGATCGAGGGTTGCCTTACGTGTCCGGACCGCAATGGCTTGGCGAGCATGTGCTTGCAGGTAAGTGGGTGCTCGC
TGTCGCCGGTACGCACGGCAAGACGACGACGAGTTCGATGCTCGCGTGGATTCTCGAAGACGCCGGCCTGAATCCGGGCT
TCCTGATCGGCGGTGTGCCGCTGAACTTCGGTGTGTCCGCGCGGCTCACCGATTCGAGCTTCTTCGTGATCGAGGCGGAC
GAGTACGACACGGCGTTCTTCGATAAGCGTTCGAAATTCGTCCATTACCGGCCTCGAACCGCGATCCTGAATAACCTCGA
ATTCGATCATGCCGATATCTTTCCAGATCTTGCCGCGATCGAGACTCAATTTCATCATTTGGTGCGCACGGTGCCGAGCG
TCGGCCGGCTCGTGACGAACGGCCGTGAAGACGCGCTCGAGCGCGTGCTGTCGCGCGGTTGCTGGAGCGATGTCGAGCGC
TTCGGCGTCGACGGCGGGTGGCAGGCGTTGCCCGCGGAGGACGGCGTGCCGGCCGACGAGCGCTTCGCCGTGTACTGGCG
CAGCGAGCGCGTCGGCGCCGTCGACTGGCACGTGCAAGGCGAACACAACCGGATGAATGCACTTGCGGCGATCGCGGCCG
CGCGTCACGTGGGCGTGCCGCCCGCGCAGGCGGCCACGGCGCTCGCGACGTTTCGCAACGTCAAGCGCCGGATGGAAGTG
CGCGGCAGCGTCGACGGCGTGACTGTCTACGACGATTTCGCACATCACCCGACCGCGATCGAGACGACGATCGCCGGGCT
TCGCGCGCGCATCGGTCGTGAAAATACTCGCATCCTCGCCGTGCTGGAGCCGCGATCGAACACGATGAAGCTCGGCGTGA
TGAAGGCGCAGCTGCCGGCGAGCCTCGCCGATGCGGATCTCGTATTCGGCTACGGCGCGCCGAGCGGCCGCGACGCGCTC
GGCTGGAGCCTGCCGGACGCGCTCGCGCCGCTCGGCGACAAGGCGCGCGCTTTCGACGATCTGCACGCGCTCGTGAAGGC
CGTGACGGTGTCGGCGCGGCCGGGCGACCACGTGCTCGTGATGAGCAACGGCGGCTTCGGCGGCGTCCACCAGAAGTTGC
TCGACGCGCTGTCTGCGCGTGGCGACGCGGCGCCGGCCCGGGGCAGCATGTGA

Upstream 100 bases:

>100_bases
GCGCGGCGTCGCGCGCGCCGCGTCGCGCATGTTTTTCCGGCAACCGGCGCAATGCGCGGCCGGTCTCCGATTCGACTACG
CCCATGCGGCGCGAAGCTCT

Downstream 100 bases:

>100_bases
TCCTGTATCTGCACGGCTTTCGTTCGTCGCCGCAGTCGTTCAAGGCGCGCGCGCTGGCTGCGCGGCTCGACGAACTCGGC
CGTGCGCACGAATGGCGCTG

Product: UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase

Products: NA

Alternate protein names: Murein peptide ligase [H]

Number of amino acids: Translated: 470; Mature: 470

Protein sequence:

>470_residues
MHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWILEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPSVGRLVTNGREDALERVLSRGCWSDVER
FGVDGGWQALPAEDGVPADERFAVYWRSERVGAVDWHVQGEHNRMNALAAIAAARHVGVPPAQAATALATFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIETTIAGLRARIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGRDAL
GWSLPDALAPLGDKARAFDDLHALVKAVTVSARPGDHVLVMSNGGFGGVHQKLLDALSARGDAAPARGSM

Sequences:

>Translated_470_residues
MHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWILEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPSVGRLVTNGREDALERVLSRGCWSDVER
FGVDGGWQALPAEDGVPADERFAVYWRSERVGAVDWHVQGEHNRMNALAAIAAARHVGVPPAQAATALATFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIETTIAGLRARIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGRDAL
GWSLPDALAPLGDKARAFDDLHALVKAVTVSARPGDHVLVMSNGGFGGVHQKLLDALSARGDAAPARGSM
>Mature_470_residues
MHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDLKPDLFVIGNVVSRGNPLMEA
ILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSMLAWILEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEAD
EYDTAFFDKRSKFVHYRPRTAILNNLEFDHADIFPDLAAIETQFHHLVRTVPSVGRLVTNGREDALERVLSRGCWSDVER
FGVDGGWQALPAEDGVPADERFAVYWRSERVGAVDWHVQGEHNRMNALAAIAAARHVGVPPAQAATALATFRNVKRRMEV
RGSVDGVTVYDDFAHHPTAIETTIAGLRARIGRENTRILAVLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGRDAL
GWSLPDALAPLGDKARAFDDLHALVKAVTVSARPGDHVLVMSNGGFGGVHQKLLDALSARGDAAPARGSM

Specific function: Reutilizes the intact tripeptide L-alanyl-gamma-D- glutamyl-meso-diaminopimelate by linking it to UDP-N-acetylmuramic acid [H]

COG id: COG0773

COG function: function code M; UDP-N-acetylmuramate-alanine ligase

Gene ontology:

Cell location: Secreted [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1790680, Length=464, Percent_Identity=58.6206896551724, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1786279, Length=305, Percent_Identity=29.1803278688525, Blast_Score=104, Evalue=1e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005757
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: 6.3.2.- [C]

Molecular weight: Translated: 50413; Mature: 50413

Theoretical pI: Translated: 6.56; Mature: 6.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECC
KPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLAAIETQFHHLVRTVPSVGRLVTNGREDALERVLSRGCWSDVER
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
FGVDGGWQALPAEDGVPADERFAVYWRSERVGAVDWHVQGEHNRMNALAAIAAARHVGVP
HCCCCCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
PAQAATALATFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRARIGRENTRILA
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
VLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGDKARAFDD
EECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHH
LHALVKAVTVSARPGDHVLVMSNGGFGGVHQKLLDALSARGDAAPARGSM
HHHHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MHIHILGICGTFMGGLAVLARAAGHTVTGCDAGVYPPMSTQLEAQGIRLIEGYGAEQIDL
CEEEEEEHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEEEEECCCCCEECC
KPDLFVIGNVVSRGNPLMEAILDRGLPYVSGPQWLGEHVLAGKWVLAVAGTHGKTTTSSM
CCCEEEEECHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCCHHHHH
LAWILEDAGLNPGFLIGGVPLNFGVSARLTDSSFFVIEADEYDTAFFDKRSKFVHYRPRT
HHHHHHHCCCCCCEEEECCCCCCCCEEEEECCEEEEEECCCCCCHHHHCCCCEEEECCHH
AILNNLEFDHADIFPDLAAIETQFHHLVRTVPSVGRLVTNGREDALERVLSRGCWSDVER
HHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHH
FGVDGGWQALPAEDGVPADERFAVYWRSERVGAVDWHVQGEHNRMNALAAIAAARHVGVP
HCCCCCCEECCCCCCCCCCCEEEEEEECCCCCEEEEEEECCCCHHHHHHHHHHHHHCCCC
PAQAATALATFRNVKRRMEVRGSVDGVTVYDDFAHHPTAIETTIAGLRARIGRENTRILA
CHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHCCCCCEEEE
VLEPRSNTMKLGVMKAQLPASLADADLVFGYGAPSGRDALGWSLPDALAPLGDKARAFDD
EECCCCCCEEEEEEEHHCCCCCCCCCEEEECCCCCCCCCCCCCCCHHHCCCCCHHHHHHH
LHALVKAVTVSARPGDHVLVMSNGGFGGVHQKLLDALSARGDAAPARGSM
HHHHHHHHEEECCCCCEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7610040; 9278503; 2843822; 7984428; 8808921 [H]