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The map label for this gene is narH-1 [H]

Identifier: 83719374

GI number: 83719374

Start: 2086546

End: 2088099

Strand: Reverse

Name: narH-1 [H]

Synonym: BTH_I1853

Alternate gene names: 83719374

Gene position: 2088099-2086546 (Counterclockwise)

Preceding gene: 83720728

Following gene: 83721571

Centisome position: 54.82

GC content: 65.77

Gene sequence:

>1554_bases
ATGAAGATCCGCGCACAAGTGGCGATGGTGCTCAATCTCGACAAATGCATCGGTTGCCACACCTGCTCCGTCACCTGCAA
GAACGTGTGGACCTCGCGCGACGGCGTCGAGTACGCGTGGTTCAACAACGTCGAGACGAAGCCCGGCGTCGGCTACCCGC
GCGAGTGGGAGAACCAGGACAAGTGGCAAGGCGGCTGGAAGCGCAACGACGCCGGCAAGCTCGAGCCGCGCCAGGGCGGC
AAGCTGAAGATCCTCGCGAACCTCTTCGCGAACCCGAACCTGCCCGCGATCGACGACTACTACGAGCCGTTCACGTACGA
CTACGCGCATCTGCAGAACGCGCCGCTGTCGCAGACGCCGCCGACCGCGCGCCCCGTGTCCGCGATCACCGGCCAGAAGA
TGGAGAAGATCGAATGGGGCCCGAACTGGGAAGACGATCTCGGCGGCGAGTTCAAGTCGCGCGGCCGCGACAAGCTGTTC
GAGGCCGTGCAAAAGGAGATGTACTCGACGTTCGAGAACACCTTCATGATGTATCTGCCGCGCCTCTGCGAGCACTGCCT
GAACCCGACCTGCGTCGCGTCGTGCCCGTCCGGCTCGGTCTACAAGCGCGAGGACGACGGCATCGTGCTCGTCGACCAGG
ACAAGTGCCGCGGCTGGCGCATGTGCATCTCCGGCTGCCCGTACAAGAAGATCTACTTCAACTGGCAGACCGGCAAGGCC
GAGAAGTGCGTGTTCTGCTTCCCGCGCATCGAGGCCGGCCAGCCGACCGTGTGCTCGGAAACCTGCGTCGGGCGCATCCG
CTATCTCGGCGTGATGCTCTATGACGCGGACCGCATCGAGCAGGCCGCGTCCGTCGCCGATCCGCAGAAGCTCTATGAAG
AGCAGCTCTCGGTGTTCGTCGATCCGCACGATCCGGCGATCGAGCGCCAGGCGCTCGCCGACGGCGTCCCGCAAAGCTGG
CTCGACGCGGCGAAGCGCTCGCCCGTCTACCGGATGGCGTGCGAATGGAAGGTCGCGTTCCCGCTGCACCCGGAATACCG
GACGCTGCCGATGGTCTGGTACGTGCCGCCCCTTTCTCCGATCCAGGGCGCCGCGCAGTCGGGCCACATGGGGATGAACG
GCGTGATTCCGGACGTGCGCAGCCTGCGGATTCCGCTGCGCTATCTCGCGAACCTGCTGACGGCGGGCGACGAAGCGCCG
GTCGCCGCCGCGCTCGACCGGATGCTCGCGATGCGCGCGTACAAGCGCGCTCAGACCGTGCACGGCGAGCACGACGCGCA
GGTGCTCGAGCAGGTCGGGCTCACCGCCGAGCAGGTCGAGGACATGTATCAGCTGATGGCGATCGCGAACTACGAGGACC
GCTTCGTGATTCCGTCGTCGCACAAGGAAATGGCCGAGGACAGCTTCGACGAGAAAGGCAGTTGCGGCTTCTCGTTCGGC
AACGGCTGCTCGGGCGGCACGTCGGACGGCACGCTGTTCGGCCGCAAGAAGGAAACGGCCGTGTCGTACGCGGCGCTGCC
GACCGTCACGCGCAAGAAAGCGAGTGCCGCATGA

Upstream 100 bases:

>100_bases
ACTACGGCACCGTCGGCAGCAACCGCGACGAATACGTGATCGTGCGCAAGATGAACCGGGTGGACTGGCTCGAAGAGCCG
CTTAACGAAGGAGCAGAACA

Downstream 100 bases:

>100_bases
GCGCATCGCACGAGGAAAACGTCATGTCGATCTACCCGATTCTGTCCGCCCTGCTGACCTACCCGGAACAGCCGCTGCTC
GACGCGCTGCCCGACATCGA

Product: nitrate reductase subunit beta

Products: NA

Alternate protein names: Nitrate reductase A subunit beta; Quinol-nitrate oxidoreductase subunit beta [H]

Number of amino acids: Translated: 517; Mature: 517

Protein sequence:

>517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGVGYPREWENQDKWQGGWKRNDAGKLEPRQGG
KLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTPPTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRGRDKLF
EAVQKEMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQTGKA
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLSVFVDPHDPAIERQALADGVPQSW
LDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAP
VAAALDRMLAMRAYKRAQTVHGEHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA

Sequences:

>Translated_517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGVGYPREWENQDKWQGGWKRNDAGKLEPRQGG
KLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTPPTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRGRDKLF
EAVQKEMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQTGKA
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLSVFVDPHDPAIERQALADGVPQSW
LDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAP
VAAALDRMLAMRAYKRAQTVHGEHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA
>Mature_517_residues
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGVGYPREWENQDKWQGGWKRNDAGKLEPRQGG
KLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTPPTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRGRDKLF
EAVQKEMYSTFENTFMMYLPRLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQTGKA
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLSVFVDPHDPAIERQALADGVPQSW
LDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSPIQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAP
VAAALDRMLAMRAYKRAQTVHGEHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA

Specific function: The nitrate reductase enzyme complex allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The beta chain is an electron transfer unit containing four cysteine clusters involved in the formation of iron-sulfur centers. Electrons ar

COG id: COG1140

COG function: function code C; Nitrate reductase beta subunit

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787478, Length=506, Percent_Identity=68.3794466403162, Blast_Score=769, Evalue=0.0,
Organism=Escherichia coli, GI1787740, Length=517, Percent_Identity=66.1508704061896, Blast_Score=760, Evalue=0.0,
Organism=Escherichia coli, GI1787872, Length=162, Percent_Identity=32.0987654320988, Blast_Score=86, Evalue=5e-18,
Organism=Escherichia coli, GI1787122, Length=132, Percent_Identity=34.0909090909091, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI2367345, Length=101, Percent_Identity=32.6732673267327, Blast_Score=66, Evalue=5e-12,
Organism=Escherichia coli, GI1787749, Length=102, Percent_Identity=33.3333333333333, Blast_Score=66, Evalue=6e-12,
Organism=Escherichia coli, GI226510944, Length=86, Percent_Identity=37.2093023255814, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1789370, Length=97, Percent_Identity=35.0515463917526, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1790326, Length=87, Percent_Identity=33.3333333333333, Blast_Score=63, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR006547 [H]

Pfam domain/function: NA

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 57925; Mature: 57925

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.5 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
3.5 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
6.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGVGYPREWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNDAGKLEPRQGGKLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRGRDKLFEAVQKEMYSTFENTFMMYLP
CCCCCHHHHHCCHHHHCCCCCCCCHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQTGKA
HHHHHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLSVFV
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHHHHEEE
DPHDPAIERQALADGVPQSWLDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCH
IQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAPVAAALDRMLAMRAYKRAQTV
HHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
HGEHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCCCC
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA
CCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHCCCC
>Mature Secondary Structure
MKIRAQVAMVLNLDKCIGCHTCSVTCKNVWTSRDGVEYAWFNNVETKPGVGYPREWENQD
CCCHHEEEEEEEHHHHHCCCEEEEEHHHHCCCCCCCEEEEECCCCCCCCCCCCCCCCCCC
KWQGGWKRNDAGKLEPRQGGKLKILANLFANPNLPAIDDYYEPFTYDYAHLQNAPLSQTP
HHCCCCCCCCCCCCCCCCCCCEEEEEEHHCCCCCCCCHHCCCCCCCCHHHHCCCCCCCCC
PTARPVSAITGQKMEKIEWGPNWEDDLGGEFKSRGRDKLFEAVQKEMYSTFENTFMMYLP
CCCCCHHHHHCCHHHHCCCCCCCCHHCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHH
RLCEHCLNPTCVASCPSGSVYKREDDGIVLVDQDKCRGWRMCISGCPYKKIYFNWQTGKA
HHHHHHCCCEEEEECCCCCCEEECCCCEEEEECCCCCCHHHHHCCCCCEEEEEEECCCCC
EKCVFCFPRIEAGQPTVCSETCVGRIRYLGVMLYDADRIEQAASVADPQKLYEEQLSVFV
CEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEECHHHHHHHHHCCCHHHHHHHHHHEEE
DPHDPAIERQALADGVPQSWLDAAKRSPVYRMACEWKVAFPLHPEYRTLPMVWYVPPLSP
CCCCCHHHHHHHHCCCCHHHHHHHHCCCCEEEEEEEEEECCCCCCCCCCCEEEECCCCCH
IQGAAQSGHMGMNGVIPDVRSLRIPLRYLANLLTAGDEAPVAAALDRMLAMRAYKRAQTV
HHCCCCCCCCCCCCCCCCHHHHHCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
HGEHDAQVLEQVGLTAEQVEDMYQLMAIANYEDRFVIPSSHKEMAEDSFDEKGSCGFSFG
CCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHCCCCCCCCCCCCC
NGCSGGTSDGTLFGRKKETAVSYAALPTVTRKKASAA
CCCCCCCCCCEEECCCHHHHHHHHHCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2674654; 8905232; 9278503; 2832376; 3053688; 1321049; 8383531; 8388253; 8664273; 9516445; 12910261 [H]