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The map label for this gene is ada [C]

Identifier: 83719257

GI number: 83719257

Start: 1902433

End: 1903488

Strand: Reverse

Name: ada [C]

Synonym: BTH_I1692

Alternate gene names: 83719257

Gene position: 1903488-1902433 (Counterclockwise)

Preceding gene: 83718591

Following gene: 83719423

Centisome position: 49.97

GC content: 70.08

Gene sequence:

>1056_bases
ATGCGATCCGAACGTCCGCAAGAGGAAGTCCGTTACTGGCGCACGCCGCTCTTGCCGGAGGCGGACTTCGTCACCGCGAC
CTATCGCGACCACGTGTTCGCGCCCCACTGGCACGACGCGTACACGATCCCCGTCATCGAGGCGGGCGCCGAACGCTACA
CGTGTCGAGGCAGCGCGCACGTCGCCGGTACGGGCTCGATCCCTGTCATCAATCCGGGGGAAATCCACACCGGCTCGCGC
GCGGCCGAAGGCGGCTGGCGCTATCGCGTTGCCTATGTACCCGTCGATTTCATTCGCGCGCTCGCCGACGAAATCGCCGG
CAAGCCGCAGCCTGCCCCATGGTTCCGGCTCGACGTCATTCACGATGCGGATCTCGCCGCGCGGCTCGTGCTCGCGCACC
GGCTGATGGAGGCGAACGGCGCCGCGGCGTTGCTCGCCGCCGCCCGCCGGCCGCGGTTCGGCGAGAGGGCCGCGACGGCC
GACGGCACCTCGGATTCGGCTTCGGACCCGTGCTCGGATTCTGCGTGGGGTTCAGCTTGGGCATCGGCTTCGGCCGCCGC
TCCGATTCCGGGCATGGATTCGGCCCGCGCTTCAGCCAGTTTTACGGCCTCGTATTCGCTGTGCACCGGCACGGCGCGGC
CGCTCGACGATCCGCTATCCGCGGAAACCGCAATGCTCGACGCATATTCGACGCTGATCGCGCGCCACGCGGGCAATCGC
AGCGCGCCGCGCGGCGTCGCGGCCGACCAGCGGCGCGTCGACGCGATGCGCGAGCGGCTGGCCGCCGATCTGTCCGAAAC
CATGACGTTATCGGATGTCGCGCGAGCGGCCGGGCTATCGCCGTTCCATGCGGCCCGCCTGTTCTCGCGCGTGGCCGGCA
TGCCGCCGCACGCATGGCGCAACCAGTTGCGCCTGCAGCGCGCGCTCGCGCCGCTGCGCGCGGGCGCGAGCGTCGCCGAT
GTCGCCGCCGCGTGCGGCTTCGCCGATCAGAGCCACTTCACTCGACATTTCAAACGAATGTTCGGCGTGCCGCCGGGGCG
TTGGCAGTCGTCGTAG

Upstream 100 bases:

>100_bases
CAACGGGCAGCCCGAAGAACAGCGTGACCGCCGTGCCCGTGCCCGCCGGCACGCCGATCCCGGTCAGGTAACGAAGCGCC
CGGTAGCCGGGTCCGCCGCC

Downstream 100 bases:

>100_bases
CGACATAGCGGCAAGCGCGCGACGCGGCTCGACCCGCGCAAATTCCAGCTTGGGCTTGGGCTCGAATCAAGGTAACGGCA
CCGGCAAAGACAGCATCAAC

Product: AraC family transcriptional regulator

Products: DNA lacking O-methylguanine; protein S-methyl-L-cysteine [C]

Alternate protein names: Transcriptional Regulator AraC Family; AraC/XylS Family Transcriptional Regulator; AraC Family Transcription Regulator; AraC/XylS Family Transcription Factor; Transcriptional Regulator; AraC Type Helix-Turn-Helix- Domain-Containing Protein; L-Rhamnose Operon Transcriptional Activator RhaR; HTH-Type Transcriptional Activator RhaS; AraC Family Regulatory Helix-Turn-Helix Protein; AraC Family Regulator; AraC-Family Regulatory Protein; Xre Family xin-Antitoxin System Antitoxin Component; Transcriptional Regulator AraC-Family; Transcriptional Regulator Protein AraC Family

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MRSERPQEEVRYWRTPLLPEADFVTATYRDHVFAPHWHDAYTIPVIEAGAERYTCRGSAHVAGTGSIPVINPGEIHTGSR
AAEGGWRYRVAYVPVDFIRALADEIAGKPQPAPWFRLDVIHDADLAARLVLAHRLMEANGAAALLAAARRPRFGERAATA
DGTSDSASDPCSDSAWGSAWASASAAAPIPGMDSARASASFTASYSLCTGTARPLDDPLSAETAMLDAYSTLIARHAGNR
SAPRGVAADQRRVDAMRERLAADLSETMTLSDVARAAGLSPFHAARLFSRVAGMPPHAWRNQLRLQRALAPLRAGASVAD
VAAACGFADQSHFTRHFKRMFGVPPGRWQSS

Sequences:

>Translated_351_residues
MRSERPQEEVRYWRTPLLPEADFVTATYRDHVFAPHWHDAYTIPVIEAGAERYTCRGSAHVAGTGSIPVINPGEIHTGSR
AAEGGWRYRVAYVPVDFIRALADEIAGKPQPAPWFRLDVIHDADLAARLVLAHRLMEANGAAALLAAARRPRFGERAATA
DGTSDSASDPCSDSAWGSAWASASAAAPIPGMDSARASASFTASYSLCTGTARPLDDPLSAETAMLDAYSTLIARHAGNR
SAPRGVAADQRRVDAMRERLAADLSETMTLSDVARAAGLSPFHAARLFSRVAGMPPHAWRNQLRLQRALAPLRAGASVAD
VAAACGFADQSHFTRHFKRMFGVPPGRWQSS
>Mature_351_residues
MRSERPQEEVRYWRTPLLPEADFVTATYRDHVFAPHWHDAYTIPVIEAGAERYTCRGSAHVAGTGSIPVINPGEIHTGSR
AAEGGWRYRVAYVPVDFIRALADEIAGKPQPAPWFRLDVIHDADLAARLVLAHRLMEANGAAALLAAARRPRFGERAATA
DGTSDSASDPCSDSAWGSAWASASAAAPIPGMDSARASASFTASYSLCTGTARPLDDPLSAETAMLDAYSTLIARHAGNR
SAPRGVAADQRRVDAMRERLAADLSETMTLSDVARAAGLSPFHAARLFSRVAGMPPHAWRNQLRLQRALAPLRAGASVAD
VAAACGFADQSHFTRHFKRMFGVPPGRWQSS

Specific function: Repair Of Alkylated Guanine In DNA By Stoichiometrically Transferring The Alkyl Group At The O-6 Position To A Cysteine Residue In The Enzyme. This Is A Suicide Reaction: The Enzyme Is Irreversibly Inactivated. Can Also Repair O-4-Methylthymine. The Meth

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.1.1.63 [C]

Molecular weight: Translated: 37752; Mature: 37752

Theoretical pI: Translated: 8.63; Mature: 8.63

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRSERPQEEVRYWRTPLLPEADFVTATYRDHVFAPHWHDAYTIPVIEAGAERYTCRGSAH
CCCCCCHHHHHHHCCCCCCCCCEEEEEHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCE
VAGTGSIPVINPGEIHTGSRAAEGGWRYRVAYVPVDFIRALADEIAGKPQPAPWFRLDVI
ECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEE
HDADLAARLVLAHRLMEANGAAALLAAARRPRFGERAATADGTSDSASDPCSDSAWGSAW
ECHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHH
ASASAAAPIPGMDSARASASFTASYSLCTGTARPLDDPLSAETAMLDAYSTLIARHAGNR
HCCCCCCCCCCCCCCCCCCCEEEEEHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SAPRGVAADQRRVDAMRERLAADLSETMTLSDVARAAGLSPFHAARLFSRVAGMPPHAWR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHH
NQLRLQRALAPLRAGASVADVAAACGFADQSHFTRHFKRMFGVPPGRWQSS
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MRSERPQEEVRYWRTPLLPEADFVTATYRDHVFAPHWHDAYTIPVIEAGAERYTCRGSAH
CCCCCCHHHHHHHCCCCCCCCCEEEEEHHCCCCCCCCCCCEEEEEEECCCCEEEECCCCE
VAGTGSIPVINPGEIHTGSRAAEGGWRYRVAYVPVDFIRALADEIAGKPQPAPWFRLDVI
ECCCCCCCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHCCCCCCCCEEEEEEE
HDADLAARLVLAHRLMEANGAAALLAAARRPRFGERAATADGTSDSASDPCSDSAWGSAW
ECHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHH
ASASAAAPIPGMDSARASASFTASYSLCTGTARPLDDPLSAETAMLDAYSTLIARHAGNR
HCCCCCCCCCCCCCCCCCCCEEEEEHEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCC
SAPRGVAADQRRVDAMRERLAADLSETMTLSDVARAAGLSPFHAARLFSRVAGMPPHAWR
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCHHHH
NQLRLQRALAPLRAGASVADVAAACGFADQSHFTRHFKRMFGVPPGRWQSS
HHHHHHHHHHHHHCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA containing O-methylguanine; protein L-cysteine; DNA [C]

Specific reaction: DNA containing O-methylguanine + protein L-cysteine = DNA lacking O-methylguanine + protein S-methyl-L-cysteine Protein + DNA = Protein-DNA [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA