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The map label for this gene is narK [H]

Identifier: 83719177

GI number: 83719177

Start: 2093491

End: 2094771

Strand: Reverse

Name: narK [H]

Synonym: BTH_I1856

Alternate gene names: 83719177

Gene position: 2094771-2093491 (Counterclockwise)

Preceding gene: 83720627

Following gene: 83721303

Centisome position: 54.99

GC content: 68.46

Gene sequence:

>1281_bases
ATGGCTGCAACCTTCGCTCCCCCCGCATCGCTCGACAAGCGCGCGATGCCCGTGCTCGCGTCGAGCACGATCGCGTTCAC
ACTGTGCTTCGCCGTCTGGATGATGTTCGCGATCCTCGGCATTCCGCTCAAGAAAACGCTCGGCCTGAACGACACCGAAT
TCGGCCTCATCGCCGCCATGCCCGTGCTCACCGGCTCGCTGATCCGCGTGCCGCTCGGCATCTGGACCGACCGCTACGGC
GGACGCATCGTGTTCTTCATCCTGATGCTCGTCACCGTCGCGCCGATCTGGCTGATCTCATATGCCACCGCGCTCTGGCA
ATTCCTCGTGCTCGGCCTGTTCGTCGGGCTCGCGGGCGGCTCGTTTTCGGTCGGCACGCCCTACGTCGCGCGCTGGTTCC
CGAAAGCGCGCCAGGGCCTCGCGATGGGCGTGTTCGGCGCCGGCAACTCGGGCGCGGCCGTCAACAAGTTCGTCGCGCCC
GCGCTGATCGCCGCGGCGGGCACGTGGACCATCGTGCCGCGCGTCTACGCGATCGCGATGCTCGCGATGGCGCTCCTCTT
CTGGCTGTTCTCCGCCACCGATCCCGCGCACCGCTCGACGAACGCGACGTCGCTGCGCGCGCAGTTGCGCGTGCTGAAGG
ACCCGCGCGTGTGGCGCTACTCGCAGTACTACTCGGTCGTGTTCGGCGGCTACGTCGGGCTGTCGCTGTGGCTCACGCAG
TACTACGTCGGCGAATACGGCTTCGGCATCCAGTCGGCCGCGTTCCTCGCCGCGTGCTTCTCGCTGCCGGGCGGCGTGCT
GCGCGCGATCGGCGGCTGGCTCTCCGACCGCTACGGCGCGTATCGCACGACGTGGTGGGTGATGTGGGTCTGCTGGGTGA
TGTTCTTCCTGCTCAGCTATCCGCCGACCGATTTCACGATCCGTGCCGCGCACGGCCCGCTCGGCTTCCATCTGTCGCTC
ACGCCCGTCGCGTTCACCGTGCTGCTGTTCGCGGTCGGCATCGCGATGGCAGTCGGCAAGGCATCGGTCTTCAAGTTCAT
CGCCGACGAGTTCCCGGACGACATCGGCGCGGTGTCGGGCGTCGTCGGCCTCGCGGGCGGCCTGGCCGGCTTCGCGCTGC
CGATCCTGTTCGGCGCGCTCGTCGACCTGACGGGCGTGCGCTCGACCTGCTTCATGCTGCTCTACGGCGCCGTCAGCGTG
AGCCTCGTCTGGATGTACTTCAGCTTCCGCGCCGAGCGCGCGGCGCACGACCAGCGCGTGCTCGCCGCCCGCTCCGCGTG
A

Upstream 100 bases:

>100_bases
CGACCCAGCGACACGAACGCGCCCGTTCCTCCGGCGAATAGGCAAGCGCATCGCCCGTCCCTACTCTTGAGACGCTCCTC
CTCGTCTCGAGAGAATCGTC

Downstream 100 bases:

>100_bases
GCGTCGTCTTCGCCATCTTCGTTCACTCAACGGATTCGATCATGTCCACCTCTCTACTCGTGCGCTGGGACCCCGAAAAC
CCGGCATTCTGGCAAGCCAA

Product: nitrate/nitrite transporter

Products: NA

Alternate protein names: Nitrite facilitator [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MAATFAPPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAMPVLTGSLIRVPLGIWTDRYG
GRIVFFILMLVTVAPIWLISYATALWQFLVLGLFVGLAGGSFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAP
ALIAAAGTWTIVPRVYAIAMLAMALLFWLFSATDPAHRSTNATSLRAQLRVLKDPRVWRYSQYYSVVFGGYVGLSLWLTQ
YYVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSYPPTDFTIRAAHGPLGFHLSL
TPVAFTVLLFAVGIAMAVGKASVFKFIADEFPDDIGAVSGVVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSV
SLVWMYFSFRAERAAHDQRVLAARSA

Sequences:

>Translated_426_residues
MAATFAPPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAMPVLTGSLIRVPLGIWTDRYG
GRIVFFILMLVTVAPIWLISYATALWQFLVLGLFVGLAGGSFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAP
ALIAAAGTWTIVPRVYAIAMLAMALLFWLFSATDPAHRSTNATSLRAQLRVLKDPRVWRYSQYYSVVFGGYVGLSLWLTQ
YYVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSYPPTDFTIRAAHGPLGFHLSL
TPVAFTVLLFAVGIAMAVGKASVFKFIADEFPDDIGAVSGVVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSV
SLVWMYFSFRAERAAHDQRVLAARSA
>Mature_425_residues
AATFAPPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAMPVLTGSLIRVPLGIWTDRYGG
RIVFFILMLVTVAPIWLISYATALWQFLVLGLFVGLAGGSFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAPA
LIAAAGTWTIVPRVYAIAMLAMALLFWLFSATDPAHRSTNATSLRAQLRVLKDPRVWRYSQYYSVVFGGYVGLSLWLTQY
YVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSYPPTDFTIRAAHGPLGFHLSLT
PVAFTVLLFAVGIAMAVGKASVFKFIADEFPDDIGAVSGVVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSVS
LVWMYFSFRAERAAHDQRVLAARSA

Specific function: Involved in excretion of nitrite produced by the dissimilatory reduction of nitrate [H]

COG id: COG2223

COG function: function code P; Nitrate/nitrite transporter

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Nitrate/nitrite porter (TC 2.A.1.8) family [H]

Homologues:

Organism=Escherichia coli, GI1787475, Length=444, Percent_Identity=25.2252252252252, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI1787743, Length=432, Percent_Identity=26.1574074074074, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 46040; Mature: 45909

Theoretical pI: Translated: 9.99; Mature: 9.99

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHCCC
>Mature Secondary Structure 
AATFAPPASLDKRAMPVLASSTIAFTLCFAVWMMFAILGIPLKKTLGLNDTEFGLIAAM
CCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHHHHH
PVLTGSLIRVPLGIWTDRYGGRIVFFILMLVTVAPIWLISYATALWQFLVLGLFVGLAGG
HHHHCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SFSVGTPYVARWFPKARQGLAMGVFGAGNSGAAVNKFVAPALIAAAGTWTIVPRVYAIAM
CCCCCCCHHHHHCHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHH
LAMALLFWLFSATDPAHRSTNATSLRAQLRVLKDPRVWRYSQYYSVVFGGYVGLSLWLTQ
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHH
YYVGEYGFGIQSAAFLAACFSLPGGVLRAIGGWLSDRYGAYRTTWWVMWVCWVMFFLLSY
HHHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
PPTDFTIRAAHGPLGFHLSLTPVAFTVLLFAVGIAMAVGKASVFKFIADEFPDDIGAVSG
CCCCEEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
VVGLAGGLAGFALPILFGALVDLTGVRSTCFMLLYGAVSVSLVWMYFSFRAERAAHDQRV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LAARSA
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8846791; 9384377; 9353933 [H]