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The map label for this gene is narI-1 [H]

Identifier: 83718779

GI number: 83718779

Start: 2085121

End: 2085807

Strand: Reverse

Name: narI-1 [H]

Synonym: BTH_I1851

Alternate gene names: 83718779

Gene position: 2085807-2085121 (Counterclockwise)

Preceding gene: 83721571

Following gene: 83721110

Centisome position: 54.76

GC content: 67.69

Gene sequence:

>687_bases
ATGGATTATCTGAATACGTTCGTCTACGGCATCTATCCGTACATCGCCGCCGCCATCTTCCTGTTCGGCAGCCTCGCGCG
CTTCGAGCGCGAGCAGTACACGTGGAAGTCCGACAGCTCGCAGCTCCTGCACCGCGGCGCGCTGCGCACGGGCAACATCC
TCTTTCACGTCGGCGTGCTCGGGCTCTTCTTCGGGCATCTGGTCGGGCTGCTCACGCCCGTGGCCGTGTGGGACGCGCTC
GGCGTCACCCACCACTTCAAGCAGCTCGTCGCGATGACGGCGGGCGGCGTGATGGGCTCGCTCGCGCTCGCGGGCCTCGC
GATCCTGCTGCACCGGCGGCTCACGAACGCGCGGATCGCGGCCGTCACGCGGCGCGGCGACAAGGTGCTGCTCGCGTGGC
TGCTCGTCACGCTCGTGCTCGGCCTGTCGACGATCTTCGAATCGGCCAGCCACACCGACGGCCATCTGATGGTGCAACTG
ATGACGTGGGCGCAGCACATCGTCACATTCCGCGGCGACGCGGCGAGCCATGTCGCCGATGCGCCGTGGCTCTTCAAGGC
GCACCTGTTCATGGGGCTCTCGCTCTTCGCGATCTTCCCGTTCACGCGCCTCGTGCATGTGTGGAGCGGCTTCGCATCGG
TCACGTACGTCGGCCGCGCGTGGCAGCTCGTGCGCGCGCGCCGCTAG

Upstream 100 bases:

>100_bases
GCCGCGCAGCCGATCCGATTTCATCCGCCGCGCCGCGCGGCGACGCCCGCTCCCCGCGCGTGACGCGGGAGGCGTCGCGA
ACCCGTTTCGAGGTCACGTC

Downstream 100 bases:

>100_bases
ACGCGGCCAGGGCGGGGGCCGAGCCAAACGCAGCGCGGCGCACCGGGCGGGTGCGCCGCGATGCCCCACGTGCTCGAATG
TCGCAGCAACCGCGCAACAT

Product: respiratory nitrate reductase subunit gamma

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 228; Mature: 228

Protein sequence:

>228_residues
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVLGLFFGHLVGLLTPVAVWDAL
GVTHHFKQLVAMTAGGVMGSLALAGLAILLHRRLTNARIAAVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQL
MTWAQHIVTFRGDAASHVADAPWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR

Sequences:

>Translated_228_residues
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVLGLFFGHLVGLLTPVAVWDAL
GVTHHFKQLVAMTAGGVMGSLALAGLAILLHRRLTNARIAAVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQL
MTWAQHIVTFRGDAASHVADAPWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR
>Mature_228_residues
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVLGLFFGHLVGLLTPVAVWDAL
GVTHHFKQLVAMTAGGVMGSLALAGLAILLHRRLTNARIAAVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQL
MTWAQHIVTFRGDAASHVADAPWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR

Specific function: This is a second nitrate reductase enzyme which can substitute for the NRA enzyme and allows E.coli to use nitrate as an electron acceptor during anaerobic growth. The gamma chain is a membrane-embedded heme-iron unit resembling cytochrome b, which transf

COG id: COG2181

COG function: function code C; Nitrate reductase gamma subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787738, Length=228, Percent_Identity=47.3684210526316, Blast_Score=204, Evalue=3e-54,
Organism=Escherichia coli, GI1787480, Length=227, Percent_Identity=46.2555066079295, Blast_Score=201, Evalue=3e-53,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003816 [H]

Pfam domain/function: PF02665 Nitrate_red_gam [H]

EC number: =1.7.99.4 [H]

Molecular weight: Translated: 25319; Mature: 25319

Theoretical pI: Translated: 10.95; Mature: 10.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
GLFFGHLVGLLTPVAVWDALGVTHHFKQLVAMTAGGVMGSLALAGLAILLHRRLTNARIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQLMTWAQHIVTFRGDAASHVAD
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCC
APWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDYLNTFVYGIYPYIAAAIFLFGSLARFEREQYTWKSDSSQLLHRGALRTGNILFHVGVL
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHHHHHHH
GLFFGHLVGLLTPVAVWDALGVTHHFKQLVAMTAGGVMGSLALAGLAILLHRRLTNARIA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHH
AVTRRGDKVLLAWLLVTLVLGLSTIFESASHTDGHLMVQLMTWAQHIVTFRGDAASHVAD
HHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHCC
APWLFKAHLFMGLSLFAIFPFTRLVHVWSGFASVTYVGRAWQLVRARR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]