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The map label for this gene is araF [H]

Identifier: 83718663

GI number: 83718663

Start: 1324913

End: 1325920

Strand: Direct

Name: araF [H]

Synonym: BTH_I1179

Alternate gene names: 83718663

Gene position: 1324913-1325920 (Clockwise)

Preceding gene: 83719365

Following gene: 83720375

Centisome position: 34.78

GC content: 67.46

Gene sequence:

>1008_bases
ATGAAACGCAGAACGTTCATCACGCTGGCGGCAGCGGCAGCGGCAGCGGCGGCGGTCGCGGCGGCGGGCCTGTCCGCGCA
CGCGGCCGAGCCCGTGAAGATCGGCTTCCTCGTCAAGCAGCCCGAGGAGCCGTGGTTCCAGGACGAATGGAAATTCGCCG
AGATCGCCGCGAAGGACAAGGGCTTCACGCTCGTGAAGATCGGCGCGCCGTCCGGCGAGAAGGTGATGAGCGCGATCGAC
AATCTCGCCGCGCAGAAGGCGCAGGGCTTCATCATCTGCACGCCGGACGTGAAGCTCGGGCCGGGCATCGTCGCGAAGGC
GAAGTCGCACGGCCTGAAGATGATGACGGTCGACGACCGGCTCGTCGACGGCGCGGGCAAGCCGATCGAATCGGTCCCGC
ACATGGGCATTTCCGCGTACGACATCGGCAAGCAGGTCGGCGGCGGGATCGCGGCCGAGATCAAGAAGCGCGGCTGGAAC
ATGAACGAAGTCGGCGCGATCGACATCACGTACGAGCAGTTGCCGACCGCGCACGACCGCACGGCGGGCGCGACCGACGC
GCTCGTCGCCGCGGGCTTTCCGAAGGCGAACGTGATCGCCGCGCCGCAGGCGAAGACCGATACCGAAAACGCGTTCAACG
CGGCGAACATCGCGCTCACGAAGAATCCGAAGTTCAAGCATTGGGTCGCCTACGGCCTGAACGACGAGGCGGTGCTCGGC
GCTGTGCGCGCGGCCGAAGGGCGCGGCTTCAAGGCGGCGGACATGATCGGCATCGGCATCGGCGGGTCGGACTCGGCGCT
CAGCGAGTTCAAGAAGCCGCAGCCGACGGGCTTCTTCGGCACCGTGATCATCAGCCCGAAGCGACACGGCGAAGAGACGT
CGGAGCTGATGTATGCGTGGATCACGCGAGGCAAGGCGCCGCCGCCGCTCACGCTCACGACGGGCATGCTCGCGACGCGC
GAGAACGTCGCGCAGGTGCGCGAGACGATGGGGCTCGCGGCGAAGTAG

Upstream 100 bases:

>100_bases
GCATACGCGCGAAGGCGGCGCGCGACGGTCGCACGCGCCGCGTCGAGACGACGAAGCGGCCGACGGCTCCGGCGACGGAG
ACAACAAGGAGACAAGCAAC

Downstream 100 bases:

>100_bases
GGGAAGCTCGCCCGCGCGCGTCGCGCGATGCGCGGTGCGCGCGGGCGACGCGGCGGGCAGGCGATAGGGCAATAAGGCAA
CCAGCACGGCAGGAAACGGA

Product: L-arabinose ABC transporter periplasmic L-arabinose-binding protein

Products: ADP; phosphate; arabinose [Cytoplasm] [C]

Alternate protein names: ABP [H]

Number of amino acids: Translated: 335; Mature: 335

Protein sequence:

>335_residues
MKRRTFITLAAAAAAAAAVAAAGLSAHAAEPVKIGFLVKQPEEPWFQDEWKFAEIAAKDKGFTLVKIGAPSGEKVMSAID
NLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKKRGWN
MNEVGAIDITYEQLPTAHDRTAGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLG
AVRAAEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITRGKAPPPLTLTTGMLATR
ENVAQVRETMGLAAK

Sequences:

>Translated_335_residues
MKRRTFITLAAAAAAAAAVAAAGLSAHAAEPVKIGFLVKQPEEPWFQDEWKFAEIAAKDKGFTLVKIGAPSGEKVMSAID
NLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKKRGWN
MNEVGAIDITYEQLPTAHDRTAGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLG
AVRAAEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITRGKAPPPLTLTTGMLATR
ENVAQVRETMGLAAK
>Mature_335_residues
MKRRTFITLAAAAAAAAAVAAAGLSAHAAEPVKIGFLVKQPEEPWFQDEWKFAEIAAKDKGFTLVKIGAPSGEKVMSAID
NLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDRLVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKKRGWN
MNEVGAIDITYEQLPTAHDRTAGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLG
AVRAAEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAWITRGKAPPPLTLTTGMLATR
ENVAQVRETMGLAAK

Specific function: Involved in the high-affinity L-arabinose membrane transport system. Binds with high affinity to arabinose, but can also bind D-galactose (approximately 2-fold reduction) and D- fucose (approximately 40-fold reduction) [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788211, Length=324, Percent_Identity=49.3827160493827, Blast_Score=321, Evalue=3e-89,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 35161; Mature: 35161

Theoretical pI: Translated: 9.69; Mature: 9.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRRTFITLAAAAAAAAAVAAAGLSAHAAEPVKIGFLVKQPEEPWFQDEWKFAEIAAKDK
CCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCC
GFTLVKIGAPSGEKVMSAIDNLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDR
CEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEHHCCCEEEEEECCH
LVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKKRGWNMNEVGAIDITYEQLPTAHDR
HHCCCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCC
TAGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLG
CCCCCHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHH
AVRAAEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAW
HHHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
ITRGKAPPPLTLTTGMLATRENVAQVRETMGLAAK
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKRRTFITLAAAAAAAAAVAAAGLSAHAAEPVKIGFLVKQPEEPWFQDEWKFAEIAAKDK
CCCEEEEEHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCCCCCCEEEEEECCC
GFTLVKIGAPSGEKVMSAIDNLAAQKAQGFIICTPDVKLGPGIVAKAKSHGLKMMTVDDR
CEEEEEECCCCCHHHHHHHHHHHHHHHCEEEEECCCCCCCCCEEEEHHCCCEEEEEECCH
LVDGAGKPIESVPHMGISAYDIGKQVGGGIAAEIKKRGWNMNEVGAIDITYEQLPTAHDR
HHCCCCCCHHHCCCCCCCHHHHHHHHCCCHHHHHHHCCCCCCCCEEEEEEHHHCCCCCCC
TAGATDALVAAGFPKANVIAAPQAKTDTENAFNAANIALTKNPKFKHWVAYGLNDEAVLG
CCCCCHHHEECCCCCCCEEECCCCCCCCCCCCCCEEEEEECCCCCCEEEEECCCCHHHHH
AVRAAEGRGFKAADMIGIGIGGSDSALSEFKKPQPTGFFGTVIISPKRHGEETSELMYAW
HHHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHH
ITRGKAPPPLTLTTGMLATRENVAQVRETMGLAAK
HHCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arabinose [Periplasm]; H2O [C]

Specific reaction: ATP + arabinose [Periplasm] + H2O = ADP + phosphate + arabinose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2445996; 9097040; 9278503; 6885805; 326784; 9298646; 7031057; 326785; 2204627 [H]