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The map label for this gene is rfbA [H]

Identifier: 83718471

GI number: 83718471

Start: 1665731

End: 1666669

Strand: Direct

Name: rfbA [H]

Synonym: BTH_I1470

Alternate gene names: 83718471

Gene position: 1665731-1666669 (Clockwise)

Preceding gene: 83719152

Following gene: 83720588

Centisome position: 43.73

GC content: 63.58

Gene sequence:

>939_bases
GTGGGTCGAAACGAACTACGCGCAACGCGCGTAAAGGCTGAATCGATGGCGCGCAAGGGCATTATTCTCGCCGGCGGCTC
CGGCACGCGGCTGTATCCGATCACGCATGCCGTGTCGAAGCAATTGCTGCCGGTCTACGACAAGCCGATGATCTACTACC
CGCTGTCGACGCTGATGGTGGCGGGCATTCGCGACGTGCTGATCATCTCGACGCCGCAGGACACGCCGCGCTTCGAGACG
ATGCTGGGCGACGGCAGTCAATGGGGGATGAGCATCCGCTACGCGGTGCAGCCGTCGCCGGACGGGCTTGCGCAGGCGTT
CATCATCGGCCGGGAATTCGTCGGCAACGATCCGTCGACGCTGATTCTCGGCGACAACATCTTCTACGGCCACGATCTCG
CGAAGCAGCTCGAGCGGGCGTCGGCGCGGCAGGCGGGCGCGACGGTGTTCGCGTATCACGTGCACGATCCGGAGCGCTAC
GGCGTCGTCGAGTTCGACCGCGAATTCCGTGCGCTGTCGATCGAGGAGAAGCCCGCGAAGCCGCGCTCGAACTACGCGGT
GACGGGGCTGTACTTCTACGACAACCAGGTGTGCGACATCGCGGCGGACATCAAGCCGTCGCCGCGCGGCGAGCTGGAGA
TCACGGACGTGAATTCCCGTTATCTCGCAGCGGGCTCGCTCGACGTCGAGATCATGGGCCGCGGCTACGCTTGGCTCGAT
ACCGGCACGCACGATTCGCTGATCGAGGCGGCGACGTTCATCGCGACGCTGCAGAAGCGCCAGGGGCTCGTCGTCGCGTG
CCCGGAGGAGATCGCGTACCGGCGGCAGTGGATCGATCAAGAGCAACTGCTCAAGCTCGCACAGCCTTTGTCGAAGAACG
GCTATGGCCAGTATCTGAAAAACATTCTCACGGACCAAGTCGCATGGCCATCCAAGTAA

Upstream 100 bases:

>100_bases
AGCCGGCCGAGACGTTCGAGACCGGCCTCGCGAAGACCGTGCGCTGGTATCTCGACAGCCAGGAGTGGGTCGACGAGGTG
GCATCGGGCGATTATCGCAA

Downstream 100 bases:

>100_bases
CTGCTACCGCTTTACCGGAAGTCAAGATCATCGAGCCGAAGGTGTTCGGCGACGCTCGCGGGTTCTTTTTCGAGAGTTTC
AATGCTCGGGAATTTTCGGA

Product: glucose-1-phosphate thymidylyltransferase

Products: NA

Alternate protein names: G1P-TT 1; dTDP-glucose pyrophosphorylase 1; dTDP-glucose synthase 1 [H]

Number of amino acids: Translated: 312; Mature: 311

Protein sequence:

>312_residues
MGRNELRATRVKAESMARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVAGIRDVLIISTPQDTPRFET
MLGDGSQWGMSIRYAVQPSPDGLAQAFIIGREFVGNDPSTLILGDNIFYGHDLAKQLERASARQAGATVFAYHVHDPERY
GVVEFDREFRALSIEEKPAKPRSNYAVTGLYFYDNQVCDIAADIKPSPRGELEITDVNSRYLAAGSLDVEIMGRGYAWLD
TGTHDSLIEAATFIATLQKRQGLVVACPEEIAYRRQWIDQEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK

Sequences:

>Translated_312_residues
MGRNELRATRVKAESMARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVAGIRDVLIISTPQDTPRFET
MLGDGSQWGMSIRYAVQPSPDGLAQAFIIGREFVGNDPSTLILGDNIFYGHDLAKQLERASARQAGATVFAYHVHDPERY
GVVEFDREFRALSIEEKPAKPRSNYAVTGLYFYDNQVCDIAADIKPSPRGELEITDVNSRYLAAGSLDVEIMGRGYAWLD
TGTHDSLIEAATFIATLQKRQGLVVACPEEIAYRRQWIDQEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK
>Mature_311_residues
GRNELRATRVKAESMARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMVAGIRDVLIISTPQDTPRFETM
LGDGSQWGMSIRYAVQPSPDGLAQAFIIGREFVGNDPSTLILGDNIFYGHDLAKQLERASARQAGATVFAYHVHDPERYG
VVEFDREFRALSIEEKPAKPRSNYAVTGLYFYDNQVCDIAADIKPSPRGELEITDVNSRYLAAGSLDVEIMGRGYAWLDT
GTHDSLIEAATFIATLQKRQGLVVACPEEIAYRRQWIDQEQLLKLAQPLSKNGYGQYLKNILTDQVAWPSK

Specific function: Catalyzes the formation of dTDP-glucose, from dTTP and glucose 1-phosphate, as well as its pyrophosphorolysis [H]

COG id: COG1209

COG function: function code M; dTDP-glucose pyrophosphorylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glucose-1-phosphate thymidylyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1788351, Length=288, Percent_Identity=72.5694444444444, Blast_Score=450, Evalue=1e-128,
Organism=Escherichia coli, GI1790224, Length=285, Percent_Identity=64.5614035087719, Blast_Score=400, Evalue=1e-113,
Organism=Escherichia coli, GI1787488, Length=235, Percent_Identity=25.9574468085106, Blast_Score=69, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI133931050, Length=182, Percent_Identity=24.7252747252747, Blast_Score=73, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6320148, Length=247, Percent_Identity=27.9352226720648, Blast_Score=85, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355443, Length=245, Percent_Identity=22.4489795918367, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI24644084, Length=245, Percent_Identity=22.4489795918367, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005907
- InterPro:   IPR005835 [H]

Pfam domain/function: PF00483 NTP_transferase [H]

EC number: =2.7.7.24 [H]

Molecular weight: Translated: 34750; Mature: 34619

Theoretical pI: Translated: 6.69; Mature: 6.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRNELRATRVKAESMARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMV
CCCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCEEEECHHHHHH
AGIRDVLIISTPQDTPRFETMLGDGSQWGMSIRYAVQPSPDGLAQAFIIGREFVGNDPST
HCCCEEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCHHHHCCCCCE
LILGDNIFYGHDLAKQLERASARQAGATVFAYHVHDPERYGVVEFDREFRALSIEEKPAK
EEEECCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCEEEEECCCCCCC
PRSNYAVTGLYFYDNQVCDIAADIKPSPRGELEITDVNSRYLAAGSLDVEIMGRGYAWLD
CCCCEEEEEEEEECCCEEEEEECCCCCCCCCEEEEECCCEEEEECCEEEEEEECCEEEEE
TGTHDSLIEAATFIATLQKRQGLVVACPEEIAYRRQWIDQEQLLKLAQPLSKNGYGQYLK
CCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
NILTDQVAWPSK
HHHHHCCCCCCC
>Mature Secondary Structure 
GRNELRATRVKAESMARKGIILAGGSGTRLYPITHAVSKQLLPVYDKPMIYYPLSTLMV
CCCHHHHHHHHHHHHHHCCEEEECCCCCEEEEEHHHHHHCCCCCCCCCEEEECHHHHHH
AGIRDVLIISTPQDTPRFETMLGDGSQWGMSIRYAVQPSPDGLAQAFIIGREFVGNDPST
HCCCEEEEEECCCCCCCHHHHCCCCCCCCEEEEEEECCCCCHHHHHHHCCHHHHCCCCCE
LILGDNIFYGHDLAKQLERASARQAGATVFAYHVHDPERYGVVEFDREFRALSIEEKPAK
EEEECCEEECHHHHHHHHHHHHHHCCCEEEEEEECCCCCCCEEEECCCEEEEECCCCCCC
PRSNYAVTGLYFYDNQVCDIAADIKPSPRGELEITDVNSRYLAAGSLDVEIMGRGYAWLD
CCCCEEEEEEEEECCCEEEEEECCCCCCCCCEEEEECCCEEEEECCEEEEEEECCEEEEE
TGTHDSLIEAATFIATLQKRQGLVVACPEEIAYRRQWIDQEQLLKLAQPLSKNGYGQYLK
CCCCHHHHHHHHHHHHHHHCCCEEEECCHHHHHHHHCCCHHHHHHHHCCCCCCCHHHHHH
NILTDQVAWPSK
HHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7517391; 9097040; 9278503; 7517390 [H]