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The map label for this gene is yjbJ [H]

Identifier: 83590448

GI number: 83590448

Start: 1643804

End: 1644424

Strand: Reverse

Name: yjbJ [H]

Synonym: Moth_1608

Alternate gene names: 83590448

Gene position: 1644424-1643804 (Counterclockwise)

Preceding gene: 83590450

Following gene: 83590447

Centisome position: 62.55

GC content: 58.94

Gene sequence:

>621_bases
ATGAATATCGAAACCCTGGCACCGGTAATAGCTGCCTGGGCGAAAATGCAGGCAACTTCTGCCAGCTTGGCTAACGAGAG
CAAGCAAAGCTTCAATACCATTTTTCTCCTGGCTCTCCTGGCCCAAATGGAACCTGACCGGGGCCTAACCCCCGCCGGGC
CAACTACTGCTGCCGGTCGCCTGGCGGACGGGTCTCAGGTGGATAGAACCGGAGCTCCCGCACCTTCTAATACGAGCCTT
GAGACTTTAATCAGCGAAGTGGCTGCCAGGTACGGTTTACCAGCGTCGCTTCTCAAAGGAGTAGTCGCGGCGGAATCCGG
CTTTAATCCCCGGGCCGTTTCTCCGGCCGGGGCCATAGGCCTCATGCAACTCATGCCGGCTACAGCCAGGGCCCTGGGGG
TAAATGACCCCTTTGACCCGGCGGCCAATCTGGACGGCGGCGCCCGTTACCTGAAACAAATGCTGGACCGCTTCCAGGGA
GATATCCGTATGGCCCTGGCGGCGTATAATGCCGGTCCCGGAGCCGTCGAGCACTACCGCGGTGTACCTCCTTACCGGGA
GACCCGGGCATATATTGATAAGGTCCTGACCGCGGCCCGCAAATTTGAGAGTTTAGTTTGA

Upstream 100 bases:

>100_bases
CAGGCTTTAAGGCCTGCTTTTTTATGACCGGATACTAGTTCAAACAGCCGTAGAGATGGCGATTATGATATAATAAACTG
AAGAAAGGGGAGAAAGTGTT

Downstream 100 bases:

>100_bases
GAAAAAAAATTATGCCGGCAGCAGGATTTTTGAGATAAATAGCGAACTAAATTTATAAATACCAAAAATGTACCTACGGC
GACGAAAGGTTATGACTCTA

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MNIETLAPVIAAWAKMQATSASLANESKQSFNTIFLLALLAQMEPDRGLTPAGPTTAAGRLADGSQVDRTGAPAPSNTSL
ETLISEVAARYGLPASLLKGVVAAESGFNPRAVSPAGAIGLMQLMPATARALGVNDPFDPAANLDGGARYLKQMLDRFQG
DIRMALAAYNAGPGAVEHYRGVPPYRETRAYIDKVLTAARKFESLV

Sequences:

>Translated_206_residues
MNIETLAPVIAAWAKMQATSASLANESKQSFNTIFLLALLAQMEPDRGLTPAGPTTAAGRLADGSQVDRTGAPAPSNTSL
ETLISEVAARYGLPASLLKGVVAAESGFNPRAVSPAGAIGLMQLMPATARALGVNDPFDPAANLDGGARYLKQMLDRFQG
DIRMALAAYNAGPGAVEHYRGVPPYRETRAYIDKVLTAARKFESLV
>Mature_206_residues
MNIETLAPVIAAWAKMQATSASLANESKQSFNTIFLLALLAQMEPDRGLTPAGPTTAAGRLADGSQVDRTGAPAPSNTSL
ETLISEVAARYGLPASLLKGVVAAESGFNPRAVSPAGAIGLMQLMPATARALGVNDPFDPAANLDGGARYLKQMLDRFQG
DIRMALAAYNAGPGAVEHYRGVPPYRETRAYIDKVLTAARKFESLV

Specific function: Murein-Degrading Enzyme. Catalyzes The Cleavage Of The Glycosidic Bonds Between N-Acetylmuramic Acid And N- Acetylglucosamine Residues In Peptidoglycan. May Play A Role In Recycling Of Muropeptides During Cell Elongation And/Or Cell Division. [C]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasmic Protein. Tightly Associated With The Murein Sacculus [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=127, Percent_Identity=34.6456692913386, Blast_Score=71, Evalue=5e-14,
Organism=Escherichia coli, GI87082191, Length=112, Percent_Identity=35.7142857142857, Blast_Score=63, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 21616; Mature: 21616

Theoretical pI: Translated: 8.83; Mature: 8.83

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIETLAPVIAAWAKMQATSASLANESKQSFNTIFLLALLAQMEPDRGLTPAGPTTAAGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LADGSQVDRTGAPAPSNTSLETLISEVAARYGLPASLLKGVVAAESGFNPRAVSPAGAIG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
LMQLMPATARALGVNDPFDPAANLDGGARYLKQMLDRFQGDIRMALAAYNAGPGAVEHYR
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
GVPPYRETRAYIDKVLTAARKFESLV
CCCCHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNIETLAPVIAAWAKMQATSASLANESKQSFNTIFLLALLAQMEPDRGLTPAGPTTAAGR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCC
LADGSQVDRTGAPAPSNTSLETLISEVAARYGLPASLLKGVVAAESGFNPRAVSPAGAIG
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCCHHHHH
LMQLMPATARALGVNDPFDPAANLDGGARYLKQMLDRFQGDIRMALAAYNAGPGAVEHYR
HHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHC
GVPPYRETRAYIDKVLTAARKFESLV
CCCCHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]