Click here to switch to the map view.

The map label for this gene is hmeA [H]

Identifier: 83590444

GI number: 83590444

Start: 1640305

End: 1641081

Strand: Reverse

Name: hmeA [H]

Synonym: Moth_1604

Alternate gene names: 83590444

Gene position: 1641081-1640305 (Counterclockwise)

Preceding gene: 83590445

Following gene: 83590443

Centisome position: 62.43

GC content: 54.57

Gene sequence:

>777_bases
ATGGAAACTAGCAGGAGGAACTTCTTAAAACTGGGTGGCGCCTGGGCCCTGGGGCTCGCCATCCTGCCGGCGGTTAAGGG
GTTCGCGGCCAACAATCCGGAACACCTGGTTAATCCGAAGGCCATGGCCGGTAAAAGGTGGGGCCTGGTCATCGATATGC
AGAAGTGCTGGCCCCGATATCAGGCAGGTTGCCGCAGGTGTTTCGATACCTGCAGGCGGGAGCACAACATTCCTGATATC
GGTAACAAGAAAGAAGAGATAAAGTGGATCTGGACGGAACCCTACGGGGATGCCTTCCCCGACCAGCAACACGAGTTGAT
GGCAGAGGACATCAAGAATAACCCCTTTATAGTTCTCTGCAACCACTGTGACAAACCGCCATGCGTGCGGGTCTGCCCCA
CCAGGGCAACCTTTAAACGCCAGGATGGTATTGTTATGATTGATTATCATCGCTGTATCGGTTGCCGTTATTGTATGGCC
GCCTGCCCATACGGGGCCAGGAGTTTCAATTTCCGGGACCCCAGGCCCTATATTAAGGAATTGAATCCTGCCTATCCCAC
CCGGGAAAAGGGCGTAGTCGAAAAGTGTACCCTCTGTGTCGAGCGGATTGATACCGGGTTGCCGCCGGCCTGCGTCGAGG
TCTGCCCGGCAAAGGCCATAACCTTTGGCGATCTGGAAGATCCCAGTTCGGAGGCAAGGCAGCTTCTGAAAAACCGGTAT
ACCATCCAGCGTAAACCCGAACTGGGCACCCATCCCAGTATTTACTATATAGTCTAG

Upstream 100 bases:

>100_bases
GTCATAGCAATAAGGCCGAGTTTTGTGACCAGTGCCATACTTATGCCGGGGTAGAGCCGGACTGCTGGTCCTGTCATATC
GAGCCAAAGGAGAATAAGTA

Downstream 100 bases:

>100_bases
TCATTTGCGAGGGAGGGGGAGAAATGATTGCCAGGGCTTTTAACGGCAGCAAAAGGTACTGGAGCTGGGTAGTATTCCTG
CTAGTCCTGGCCGGCATAGG

Product: 4Fe-4S ferredoxin, iron-sulfur binding

Products: oxidized cytochrome C552; NH3 [C]

Alternate protein names: Hme subunit A [H]

Number of amino acids: Translated: 258; Mature: 258

Protein sequence:

>258_residues
METSRRNFLKLGGAWALGLAILPAVKGFAANNPEHLVNPKAMAGKRWGLVIDMQKCWPRYQAGCRRCFDTCRREHNIPDI
GNKKEEIKWIWTEPYGDAFPDQQHELMAEDIKNNPFIVLCNHCDKPPCVRVCPTRATFKRQDGIVMIDYHRCIGCRYCMA
ACPYGARSFNFRDPRPYIKELNPAYPTREKGVVEKCTLCVERIDTGLPPACVEVCPAKAITFGDLEDPSSEARQLLKNRY
TIQRKPELGTHPSIYYIV

Sequences:

>Translated_258_residues
METSRRNFLKLGGAWALGLAILPAVKGFAANNPEHLVNPKAMAGKRWGLVIDMQKCWPRYQAGCRRCFDTCRREHNIPDI
GNKKEEIKWIWTEPYGDAFPDQQHELMAEDIKNNPFIVLCNHCDKPPCVRVCPTRATFKRQDGIVMIDYHRCIGCRYCMA
ACPYGARSFNFRDPRPYIKELNPAYPTREKGVVEKCTLCVERIDTGLPPACVEVCPAKAITFGDLEDPSSEARQLLKNRY
TIQRKPELGTHPSIYYIV
>Mature_258_residues
METSRRNFLKLGGAWALGLAILPAVKGFAANNPEHLVNPKAMAGKRWGLVIDMQKCWPRYQAGCRRCFDTCRREHNIPDI
GNKKEEIKWIWTEPYGDAFPDQQHELMAEDIKNNPFIVLCNHCDKPPCVRVCPTRATFKRQDGIVMIDYHRCIGCRYCMA
ACPYGARSFNFRDPRPYIKELNPAYPTREKGVVEKCTLCVERIDTGLPPACVEVCPAKAITFGDLEDPSSEARQLLKNRY
TIQRKPELGTHPSIYYIV

Specific function: Has menaquinol-oxidizing activity. HmeA, hmeB and hmeE subunits may together catalyze electron transfer from menaquinol to cytochrome c [H]

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Extracellular side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367345, Length=259, Percent_Identity=35.5212355212355, Blast_Score=140, Evalue=9e-35,
Organism=Escherichia coli, GI226510944, Length=256, Percent_Identity=29.6875, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1787872, Length=182, Percent_Identity=33.5164835164835, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789370, Length=252, Percent_Identity=33.3333333333333, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1787122, Length=182, Percent_Identity=31.3186813186813, Blast_Score=102, Evalue=3e-23,
Organism=Escherichia coli, GI1787749, Length=116, Percent_Identity=37.9310344827586, Blast_Score=82, Evalue=2e-17,
Organism=Escherichia coli, GI1790326, Length=192, Percent_Identity=30.7291666666667, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787740, Length=159, Percent_Identity=32.7044025157233, Blast_Score=81, Evalue=5e-17,
Organism=Escherichia coli, GI1787478, Length=108, Percent_Identity=37.962962962963, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1789067, Length=190, Percent_Identity=31.5789473684211, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI2367245, Length=109, Percent_Identity=39.4495412844037, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788811, Length=105, Percent_Identity=36.1904761904762, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=191, Percent_Identity=27.2251308900524, Blast_Score=68, Evalue=6e-13,
Organism=Escherichia coli, GI87082114, Length=147, Percent_Identity=30.6122448979592, Blast_Score=62, Evalue=4e-11,
Organism=Escherichia coli, GI87082179, Length=105, Percent_Identity=32.3809523809524, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006311 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 29494; Mature: 29494

Theoretical pI: Translated: 8.53; Mature: 8.53

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

6.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
8.5 %Cys+Met (Translated Protein)
6.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
8.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METSRRNFLKLGGAWALGLAILPAVKGFAANNPEHLVNPKAMAGKRWGLVIDMQKCWPRY
CCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCEEEEHHHHCHHH
QAGCRRCFDTCRREHNIPDIGNKKEEIKWIWTEPYGDAFPDQQHELMAEDIKNNPFIVLC
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEC
NHCDKPPCVRVCPTRATFKRQDGIVMIDYHRCIGCRYCMAACPYGARSFNFRDPRPYIKE
CCCCCCCCEEECCCCHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LNPAYPTREKGVVEKCTLCVERIDTGLPPACVEVCPAKAITFGDLEDPSSEARQLLKNRY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCC
TIQRKPELGTHPSIYYIV
CEEECCCCCCCCCEEEEC
>Mature Secondary Structure
METSRRNFLKLGGAWALGLAILPAVKGFAANNPEHLVNPKAMAGKRWGLVIDMQKCWPRY
CCCCHHHHHHHCCHHHHHHHHHHHHHHHCCCCCHHHCCCHHHCCCCCCEEEEHHHHCHHH
QAGCRRCFDTCRREHNIPDIGNKKEEIKWIWTEPYGDAFPDQQHELMAEDIKNNPFIVLC
HHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHCCCCEEEEC
NHCDKPPCVRVCPTRATFKRQDGIVMIDYHRCIGCRYCMAACPYGARSFNFRDPRPYIKE
CCCCCCCCEEECCCCHHHHCCCCEEEEEHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHH
LNPAYPTREKGVVEKCTLCVERIDTGLPPACVEVCPAKAITFGDLEDPSSEARQLLKNRY
CCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCCCEEEECCCCCCHHHHHHHHHHCC
TIQRKPELGTHPSIYYIV
CEEECCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: reduced cytochrome C552; nitrite [C]

Specific reaction: reduced cytochrome C552 + nitrite = oxidized cytochrome C552 + NH3 [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9389475; 11952791 [H]