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The map label for this gene is sigK [H]

Identifier: 83590422

GI number: 83590422

Start: 1616288

End: 1616968

Strand: Reverse

Name: sigK [H]

Synonym: Moth_1582

Alternate gene names: 83590422

Gene position: 1616968-1616288 (Counterclockwise)

Preceding gene: 83590423

Following gene: 83590421

Centisome position: 61.51

GC content: 47.14

Gene sequence:

>681_bases
ATGGAAGCAGGTTTTGTTGCTCTGGTTGCCCTTTCTGTTTTAAAGGGCATTAGTTTACTACTGTCGTATATCAGCAATAA
TGCCTTTCCCCAGCCCCTGTCTGAAGAAGAAGAGCAAAAATACCTGGAACTCTGGCTGCAGGGCGATCAAAAGGCCCGTA
ATATTCTAATTGAACATAACCTGCGCCTGGTGGCCCACATAACCAAAAAATTTGAGAACACCGGTGAGGATACCGAAGAT
CTGATATCCATAGGCACCGTTGGGCTTATAAAAGCTATTAACACTTATAATAAGGCTAAAGGCACCAAACTGGCCACCTA
TGCAGCCCGTTGCATAGAGAATGAGATCCTCATGCATTTAAGGACCCTTAAAAAGACACGGGGAGAAATTTCCCTCTATG
ACCCAATTGGCGTTGACCGCGAAGGCAATGAGATCGCCCTCATCGACATTCTAGGCTCGGAACAGGACGTAGCCGAAATG
GTAGAATCTTCTTACGAACAGCAAAGAGTGCGGCAAAAGATAAGCACCCTCACCCCTAGGGAGAGAAAGGTTCTGGAGAT
GCGTTACGGGCTATGGCATGGTTTACGCCGCACCCAGAGGGATATAGCCAGGAAGATGGGCATTTCCCGTTCCTATGTTT
CCCGCATCGAGAAGCCTGCTCATATACATACAACTTCCTAA

Upstream 100 bases:

>100_bases
AATAAATGGAGGGGCTAAGGCGGAACCTGGACAAGCACCAATTGTACCTAGCTTCCATATATATAAAGGGTAAAGCCATC
CTTCCCGGGGGGTATACAAT

Downstream 100 bases:

>100_bases
TACCTGCAAAAACCAAATAGTTTCCCAATAGGTTTGCCTGTTGGCGCGGTCTTGGCCGCGCTATATTTTTTAACTGGACG
GTTTTTTATGATTGCGGTAA

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 226; Mature: 226

Protein sequence:

>226_residues
MEAGFVALVALSVLKGISLLLSYISNNAFPQPLSEEEEQKYLELWLQGDQKARNILIEHNLRLVAHITKKFENTGEDTED
LISIGTVGLIKAINTYNKAKGTKLATYAARCIENEILMHLRTLKKTRGEISLYDPIGVDREGNEIALIDILGSEQDVAEM
VESSYEQQRVRQKISTLTPRERKVLEMRYGLWHGLRRTQRDIARKMGISRSYVSRIEKPAHIHTTS

Sequences:

>Translated_226_residues
MEAGFVALVALSVLKGISLLLSYISNNAFPQPLSEEEEQKYLELWLQGDQKARNILIEHNLRLVAHITKKFENTGEDTED
LISIGTVGLIKAINTYNKAKGTKLATYAARCIENEILMHLRTLKKTRGEISLYDPIGVDREGNEIALIDILGSEQDVAEM
VESSYEQQRVRQKISTLTPRERKVLEMRYGLWHGLRRTQRDIARKMGISRSYVSRIEKPAHIHTTS
>Mature_226_residues
MEAGFVALVALSVLKGISLLLSYISNNAFPQPLSEEEEQKYLELWLQGDQKARNILIEHNLRLVAHITKKFENTGEDTED
LISIGTVGLIKAINTYNKAKGTKLATYAARCIENEILMHLRTLKKTRGEISLYDPIGVDREGNEIALIDILGSEQDVAEM
VESSYEQQRVRQKISTLTPRERKVLEMRYGLWHGLRRTQRDIARKMGISRSYVSRIEKPAHIHTTS

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=249, Percent_Identity=25.7028112449799, Blast_Score=72, Evalue=3e-14,
Organism=Escherichia coli, GI1789871, Length=105, Percent_Identity=35.2380952380952, Blast_Score=61, Evalue=5e-11,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 25720; Mature: 25720

Theoretical pI: Translated: 8.89; Mature: 8.89

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAGFVALVALSVLKGISLLLSYISNNAFPQPLSEEEEQKYLELWLQGDQKARNILIEHN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC
LRLVAHITKKFENTGEDTEDLISIGTVGLIKAINTYNKAKGTKLATYAARCIENEILMHL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RTLKKTRGEISLYDPIGVDREGNEIALIDILGSEQDVAEMVESSYEQQRVRQKISTLTPR
HHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
ERKVLEMRYGLWHGLRRTQRDIARKMGISRSYVSRIEKPAHIHTTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEAGFVALVALSVLKGISLLLSYISNNAFPQPLSEEEEQKYLELWLQGDQKARNILIEHN
CCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHCC
LRLVAHITKKFENTGEDTEDLISIGTVGLIKAINTYNKAKGTKLATYAARCIENEILMHL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
RTLKKTRGEISLYDPIGVDREGNEIALIDILGSEQDVAEMVESSYEQQRVRQKISTLTPR
HHHHHHCCCEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCH
ERKVLEMRYGLWHGLRRTQRDIARKMGISRSYVSRIEKPAHIHTTS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]