Click here to switch to the map view.

The map label for this gene is epsE [H]

Identifier: 83590397

GI number: 83590397

Start: 1596760

End: 1598421

Strand: Reverse

Name: epsE [H]

Synonym: Moth_1554

Alternate gene names: 83590397

Gene position: 1598421-1596760 (Counterclockwise)

Preceding gene: 83590398

Following gene: 83590396

Centisome position: 60.8

GC content: 62.27

Gene sequence:

>1662_bases
ATGGATAGTCGACGACGACTGGGGGACCTGTTGATCGAAGCCGGGATGCTTACCCCGGCCCAGCTGGAACAGGCCCTGCA
GGAACAGAAACGCAGCGGGGAGCGCCTGGGTAAGGTTTTAATCCGCCTGGGATTTATCACCGAGGCCAGCATGCTGGAGG
TCCTGGAGTTCCAGCTGGGGATCCCCAAGGTGGTCCTGGCTGACTACCACCTGGATCCGGAGGTGGTCCGCCTGGTGCCG
GAAGGCCTGGCCCGGCGCTACCAGGCCATCCCCATCCGCCTGGACGGCAACCGCCTCCTGGTGGCCATGGCCGATCCCCT
GAACCTCGTGGCCCTGGACGACCTGCGCCTGGTCACCGGCAAGGAGATTATGCCGGCTATAGCCGCCGAGAAGGAAATCG
AGGCAGCTTTAAGCCGGTTCTGGCAACGGGAACCCGTTACGAGCATGAGCGAAGTAGCGGCAGCCGTCGCCGCCGCGGAA
TCTGGCGGGCGCGCCGGCGGCACGGAAGGCGCGCCGGCTGTGCGCCTGGTCAACAGTTTTATCCAGCAGGCCATCCAGAC
CCGGGCCAGCGACATCCATATAGAGCCCCAGGAGGGGGAGGTCCGGGTGCGCCTGCGGGTAGACGGCCTGCTGCGGGAGT
TGACCCGCCTGCCCCTGGGGGTTTTAAGTAGCCTGATCTCCAGGATCAAGATCATGGCCGGCATGGACATCGCCGAAAAA
CGCTTGCCCCAGGACGGCCGTTTTCAGTTTACCCTGGGTAAACGCAGTGTCGACCTCAGGGTTTCCAGCCTGCCTACTGT
TTACGGCGAAAAGATCGTCCTGCGCCTCCTGGACCAGGAGGCCATGCTCCTGCCCCTGGACGACCTGGGATTTTTGCCGG
CCATAAAAGAACGCTTTGAGAGTCTCATCCACAGTTCCTACGGCATGCTCCTCATTACCGGTCCCACGGGCAGCGGTAAG
ACGACGACCCTTTATGCTACTCTTAACATTTTAAGCTCGCCGGAAAAAAATATCATTACCATTGAGGATCCGGTAGAATA
CCTGCTGCCCGGCATCAATCAGGTGCGGGTTAACCCCAAGGCCGGCCTGACCTTTGCTTCAGGGCTGCGTTCCATCCTGC
GTCAGGACCCGGATATCATTATGGTCGGGGAGATTCGCGACCGGGAGACGGCCGATATCGCCGTCCGGGCGGCGACTACC
GGTCACCTGGTCTTAACGACCCTGCACACCAATGACGCCGCCGGCGCCGTAACCCGCCTCCTGGATATGGGAGTGGAAGG
CTACCTGGTCAATTCCTCCCTTATTGGCGTGGTGGCCCAGCGCCTGGTGCGCCGCATCTGTCCCCATTGCCGGGAGATGT
ACGAGCCGGAGCCGGGCTCTCCGGAAAGGGCCTGGTTGCCGGGCGCGGAACGGCTCTGGCGCGGCCGGGGTTGCGAAAAC
TGCCATTATACCGGTTACACCAACCGGACGGCCATCCAGGAGGTCCTGGTCATGAATGAAGAACTCCGGCGCCTGGTAGC
CGCCAAGGCGCCGGCTACGGCCCTGAAGGAGGCAGCGGTGGCCGGCGGTATGGTTCCTTTGATTGACGACGGTTTGGAAA
AAGCCCGCCAGGGGATCACTACGGTGAGCGAGGTCCTACGCGTTTCCCTGGGAGGTTTGTAA

Upstream 100 bases:

>100_bases
TCCTGCCGGAAAGCCTGGGCCGGGTGCGTCTTGTCCCCGTCAGTATCGAGGAAATCGCGGCGGTGGTTGAAAACGGTGCG
GCAGCGGAGGGGTGAGTCAT

Downstream 100 bases:

>100_bases
TGGGAGGGTCAAGATGCTCGATATCGAAACAATCCTGGTAGCGGCTGCCGCCGCCGGTGCCTCCGACGTCCATATCTCTG
TTGGGCTACCGCCGGTTTTC

Product: type II secretion system protein E

Products: NA

Alternate protein names: Cholera toxin secretion protein epsE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 553; Mature: 553

Protein sequence:

>553_residues
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLGIPKVVLADYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTGKEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAE
SGGRAGGTEGAPAVRLVNSFIQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFESLIHSSYGMLLITGPTGSGK
TTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPKAGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATT
GHLVLTTLHTNDAAGAVTRLLDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGAERLWRGRGCEN
CHYTGYTNRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMVPLIDDGLEKARQGITTVSEVLRVSLGGL

Sequences:

>Translated_553_residues
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLGIPKVVLADYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTGKEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAE
SGGRAGGTEGAPAVRLVNSFIQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFESLIHSSYGMLLITGPTGSGK
TTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPKAGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATT
GHLVLTTLHTNDAAGAVTRLLDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGAERLWRGRGCEN
CHYTGYTNRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMVPLIDDGLEKARQGITTVSEVLRVSLGGL
>Mature_553_residues
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLGIPKVVLADYHLDPEVVRLVP
EGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTGKEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAE
SGGRAGGTEGAPAVRLVNSFIQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFESLIHSSYGMLLITGPTGSGK
TTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPKAGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATT
GHLVLTTLHTNDAAGAVTRLLDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGAERLWRGRGCEN
CHYTGYTNRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMVPLIDDGLEKARQGITTVSEVLRVSLGGL

Specific function: Required for secretion of cholera toxin through the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=389, Percent_Identity=51.1568123393316, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1786296, Length=467, Percent_Identity=40.2569593147752, Blast_Score=343, Evalue=2e-95,
Organism=Escherichia coli, GI87082188, Length=186, Percent_Identity=43.5483870967742, Blast_Score=124, Evalue=1e-29,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 60459; Mature: 60459

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPKVVLADYHLDPEVVRLVPEGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTG
CCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCEEEECCEEEECC
KEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAESGGRAGGTEGAPAVRLVNSF
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
IQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
HHHHHHCCCCCEEECCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFE
HCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECHHCCCHHHHHHHHH
SLIHSSYGMLLITGPTGSGKTTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPK
HHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCEEEECCCHHHHCCCCCEEEECCC
AGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATTGHLVLTTLHTNDAAGAVTRL
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHH
LDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGAERLWRGRGCEN
HHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCC
CHYTGYTNRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMVPLIDDGLEKARQGIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TVSEVLRVSLGGL
HHHHHHHHHHCCC
>Mature Secondary Structure
MDSRRRLGDLLIEAGMLTPAQLEQALQEQKRSGERLGKVLIRLGFITEASMLEVLEFQLG
CCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
IPKVVLADYHLDPEVVRLVPEGLARRYQAIPIRLDGNRLLVAMADPLNLVALDDLRLVTG
CCEEEEECCCCCHHHHHHHHHHHHHHHEEEEEEECCCEEEEEECCCCCEEEECCEEEECC
KEIMPAIAAEKEIEAALSRFWQREPVTSMSEVAAAVAAAESGGRAGGTEGAPAVRLVNSF
CHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
IQQAIQTRASDIHIEPQEGEVRVRLRVDGLLRELTRLPLGVLSSLISRIKIMAGMDIAEK
HHHHHHCCCCCEEECCCCCCEEEEEEHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHHHH
RLPQDGRFQFTLGKRSVDLRVSSLPTVYGEKIVLRLLDQEAMLLPLDDLGFLPAIKERFE
HCCCCCCEEEEECCCEEEEEECCCCCHHHHHHHHHHHCCCCEEEECHHCCCHHHHHHHHH
SLIHSSYGMLLITGPTGSGKTTTLYATLNILSSPEKNIITIEDPVEYLLPGINQVRVNPK
HHHHCCCCEEEEECCCCCCCEEEEEEEEEECCCCCCCEEEECCCHHHHCCCCCEEEECCC
AGLTFASGLRSILRQDPDIIMVGEIRDRETADIAVRAATTGHLVLTTLHTNDAAGAVTRL
CCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEEECCCEEEEEEEECCCHHHHHHHH
LDMGVEGYLVNSSLIGVVAQRLVRRICPHCREMYEPEPGSPERAWLPGAERLWRGRGCEN
HHCCCCCEEECHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHCCCCCCC
CHYTGYTNRTAIQEVLVMNEELRRLVAAKAPATALKEAAVAGGMVPLIDDGLEKARQGIT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TVSEVLRVSLGGL
HHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8423007; 10952301 [H]