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The map label for this gene is pilT [H]

Identifier: 83590396

GI number: 83590396

Start: 1595727

End: 1596746

Strand: Reverse

Name: pilT [H]

Synonym: Moth_1553

Alternate gene names: 83590396

Gene position: 1596746-1595727 (Counterclockwise)

Preceding gene: 83590397

Following gene: 83590395

Centisome position: 60.74

GC content: 60.88

Gene sequence:

>1020_bases
ATGCTCGATATCGAAACAATCCTGGTAGCGGCTGCCGCCGCCGGTGCCTCCGACGTCCATATCTCTGTTGGGCTACCGCC
GGTTTTCCGGGTGCACGGCGAACTCCAGGTCCAGCGCCAGTGGGACCCCCTGGATTTGGAGATGACAGCCCGCCTGGTAC
GGCCTATAGTGGGCGATAAGTGGGAGGTATTTCAGGAGCAAGGTGAAATCGATCTGGCTTACTCCCTGCCCGGTGTAAGC
CGTTTCCGGGTCAATGTCTTTCACCAGCGGGGGAGCGTCGGGGCGGCCATCCGCCTTATCCCCAGGGAGATACCCAGCCT
GGAGTCCCTGGGTTTACCGCCGGTGGTGGCCGAACTGGCGGGCAGACAGCACGGCCTGATCCTGGTGACGGGGCCGACGG
GCAGCGGTAAATCCACCACCCTGGCGGCCATGGTGGATAAAATCAACCGGGAGCGGAGCTGTCACATCATCACCCTGGAA
GACCCCATTGAGTACTTGCACCAGCACCGCCGCAGCATAGTCAACCAGCGGGAAGTGGGTTCTGATACCAGGTCCTTTGC
CAGCGCCCTGCGGGCCGCCCTGCGTCAGGACCCCGACGTTATCCTGGTCGGGGAGATGCGCGACCTGGAAACCATCGCCA
CGGCCATTACGGCTGCCGAAACAGGTCACCTGGTCCTGGCGACTTTGCACACCAGCAGTGCCGTCCAGAGTGTGGATCGG
ATCATTGACGTTTTCCCGCCCCACCAGCAGGGGCAGGTACGGATCCAGCTTGCCGACACCCTGGAAGGGGTGATCACCCA
GCAGCTTTTACCGCGGGCCGACAGGAAGGGCCGGGTGGCGGCTGTAGAAGTGTTGATAGCCACCCCGGCAGTGAAGAATC
TCATCCGCGAGGGTAAAACCCATCAAATCGTTTCCACTATGCAGACCGGAGCCCGCTACGGGATGCAGACCATGGAGATG
GCGCTGCGGCAACTGATCACCCGGGGAGTAATTTTTGAGGAAGGTTTAAATATTGGTTAA

Upstream 100 bases:

>100_bases
CCTTTGATTGACGACGGTTTGGAAAAAGCCCGCCAGGGGATCACTACGGTGAGCGAGGTCCTACGCGTTTCCCTGGGAGG
TTTGTAATGGGAGGGTCAAG

Downstream 100 bases:

>100_bases
GGACACCGGCTAAAGGAGGATTCTGGCGGTTTGCGGCGAATAGAAAAATATCCTTGTTAAGGGCAAACCTGGTGAAAGCC
AGGGGCGCAAAGCCACGGGC

Product: twitching motility protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 339

Protein sequence:

>339_residues
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDKWEVFQEQGEIDLAYSLPGVS
RFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELAGRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSTMQTGARYGMQTMEM
ALRQLITRGVIFEEGLNIG

Sequences:

>Translated_339_residues
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDKWEVFQEQGEIDLAYSLPGVS
RFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELAGRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSTMQTGARYGMQTMEM
ALRQLITRGVIFEEGLNIG
>Mature_339_residues
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDKWEVFQEQGEIDLAYSLPGVS
RFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELAGRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLE
DPIEYLHQHRRSIVNQREVGSDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKTHQIVSTMQTGARYGMQTMEM
ALRQLITRGVIFEEGLNIG

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=330, Percent_Identity=47.2727272727273, Blast_Score=290, Evalue=9e-80,
Organism=Escherichia coli, GI1789723, Length=283, Percent_Identity=37.1024734982332, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI1786296, Length=302, Percent_Identity=33.4437086092715, Blast_Score=117, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 37048; Mature: 37048

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDK
CCCHHHHHHHHHCCCCCCEEEEECCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCH
WEVFQEQGEIDLAYSLPGVSRFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELA
HHHHHHCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEECHHHCCCHHHCCCCHHHHHHC
GRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLEDPIEYLHQHRRSIVNQREVG
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCC
SDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
CHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKT
HHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHCHHHHHHHHCCCH
HQIVSTMQTGARYGMQTMEMALRQLITRGVIFEEGLNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC
>Mature Secondary Structure
MLDIETILVAAAAAGASDVHISVGLPPVFRVHGELQVQRQWDPLDLEMTARLVRPIVGDK
CCCHHHHHHHHHCCCCCCEEEEECCCCEEEECCEEEEEECCCCCCHHHHHHHHHHHHCCH
WEVFQEQGEIDLAYSLPGVSRFRVNVFHQRGSVGAAIRLIPREIPSLESLGLPPVVAELA
HHHHHHCCCEEEEEECCCCCEEEEEEEECCCCCCCEEEECHHHCCCHHHCCCCHHHHHHC
GRQHGLILVTGPTGSGKSTTLAAMVDKINRERSCHIITLEDPIEYLHQHRRSIVNQREVG
CCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCC
SDTRSFASALRAALRQDPDVILVGEMRDLETIATAITAAETGHLVLATLHTSSAVQSVDR
CHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCCEEEEEECCHHHHHHHHH
IIDVFPPHQQGQVRIQLADTLEGVITQQLLPRADRKGRVAAVEVLIATPAVKNLIREGKT
HHHCCCCCCCCEEEEEEHHHHHHHHHHHHHCCCCCCCCEEEEEEEHHCHHHHHHHHCCCH
HQIVSTMQTGARYGMQTMEMALRQLITRGVIFEEGLNIG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]