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The map label for this gene is pmbA [H]

Identifier: 83590384

GI number: 83590384

Start: 1584022

End: 1585374

Strand: Reverse

Name: pmbA [H]

Synonym: Moth_1541

Alternate gene names: 83590384

Gene position: 1585374-1584022 (Counterclockwise)

Preceding gene: 83590385

Following gene: 83590383

Centisome position: 60.31

GC content: 61.64

Gene sequence:

>1353_bases
ATGGATTACCAGGAACTAGAGAAAAAATACCTGGACCTGGCCGGTCAGGTGGTCGAGAAAGCGGCCAAACGAGGAGTCCT
GGCAGAAGCCTACCTTACTGCCGGGGAAGAACTGAGCATTGAGGTCCGGGACCAGCAGGTCGAGGCCCTGACCACAGCCC
GGGATCAGGGCCTGGGCTTAAGGGTTATCCGGGACCACCGGGTAGGTTTCGCCTTTACCACCGACTTCAGCCCGGCGGCC
CTCGACGCCTGCATCGAACAGGCCCTGGCCAACGCCCGGATGGCCACTCCTGATGAGCACAACTGCCTGCCGGCCCGCTA
TCCCGGTTATCCGGCCCTGGACCTCTGGGATCCCGAGATTACCGCTACGCCCCTGGAGAAAAAGATTGAGTTAGCCAAAG
AGATTGAGCGCCAGGCCAGGGCCTATGACCCGCGGGTCAAGATAACGGAAAGTTGTTCCTATAATGACTCCCGCTACCTG
GTGGCCCTGGCGAACTCCCAGGGAATAACGGCAGCCTATCACGCTGCCAACTGTGGCGCCAGCACCTTTGTGGTGGCAGT
AGAAAATGGAGAAAGCCAGACCGGCTTCGGCCTGGCCTACGGGTTGAAGTTCAAAAACATCGACCCTGCCAAGGTGGGCC
GGGAGGGGGCCAGCAAGGCCGTACGCATGCTGGGGGCCAAAAGGGTCAATACCCAGCGGGCAGCGGTTGTCTTTGACCCT
TACGTGGCCACCAACTTCCTGGGCGTCATCGCCCCGGCCCTGGCCGCCGATGCCGTCCAGAAGGGCAAATCCCTCTTCCG
CGGCCGGGTCGGCCAGCAGGTAGCCGCGCCGGTGATCAACCTCATCGACGACGGTTGCCGGCCGGACGGCATTGCCTCCA
GCCCCTTTGACGGGGAAGGGGTGCCCACGGAACATACCGTCCTGATTGAAAAGGGCGTTTTGCGGTGCTTCCTCCATAAT
ACCTACACCGCCGCCCGGGACGGGGTGAGATCCACCGGTAACGGTGCCCGGGGTTCCTTCAAGACCACGCCTGAAGTCGG
CACCACCAATTTCTATATCGAGGCCGGATCGCGCTCGCCGGAAGAAATCATCAAGGAGATTCCAAAGGGTCTCTACGTCA
CTGAGGTCATGGGCATGCACACGGCCAACCCCATTTCCGGGGATTTCTCCGTCGGCGCCACCGGTATCTGGATCGAAAAG
GGCGAGTTGACCACGCCGGTGCGGGGGGTGGCCATTGCCGGAAACATCATTGGCCTCCTGGAGGCCATTGACGCCGTGGC
CAACGACCTGACCTTTTTCGGTGCCACCGGCGCCCCCACCATCCGGATCGCCAGCATGACCATCAGCGGCTAA

Upstream 100 bases:

>100_bases
GCAAAGACGGCCAGGGTGTTCCGGTCGGGGACGCGCAGCCAACCATCAGGATCCCCGAGCTGGTAGTTGGCGGTATCCTG
GATGAGGGGGATAAAGAATA

Downstream 100 bases:

>100_bases
AGGAAAAAGTAATTTATAAAGCAGGACCTACCTAAACGAGCAATATTATGGTAAAATAGCAGCCATGAAGATTCTAGTTA
TAAACGGGCCCAACCTGAAC

Product: peptidase U62, modulator of DNA gyrase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MDYQELEKKYLDLAGQVVEKAAKRGVLAEAYLTAGEELSIEVRDQQVEALTTARDQGLGLRVIRDHRVGFAFTTDFSPAA
LDACIEQALANARMATPDEHNCLPARYPGYPALDLWDPEITATPLEKKIELAKEIERQARAYDPRVKITESCSYNDSRYL
VALANSQGITAAYHAANCGASTFVVAVENGESQTGFGLAYGLKFKNIDPAKVGREGASKAVRMLGAKRVNTQRAAVVFDP
YVATNFLGVIAPALAADAVQKGKSLFRGRVGQQVAAPVINLIDDGCRPDGIASSPFDGEGVPTEHTVLIEKGVLRCFLHN
TYTAARDGVRSTGNGARGSFKTTPEVGTTNFYIEAGSRSPEEIIKEIPKGLYVTEVMGMHTANPISGDFSVGATGIWIEK
GELTTPVRGVAIAGNIIGLLEAIDAVANDLTFFGATGAPTIRIASMTISG

Sequences:

>Translated_450_residues
MDYQELEKKYLDLAGQVVEKAAKRGVLAEAYLTAGEELSIEVRDQQVEALTTARDQGLGLRVIRDHRVGFAFTTDFSPAA
LDACIEQALANARMATPDEHNCLPARYPGYPALDLWDPEITATPLEKKIELAKEIERQARAYDPRVKITESCSYNDSRYL
VALANSQGITAAYHAANCGASTFVVAVENGESQTGFGLAYGLKFKNIDPAKVGREGASKAVRMLGAKRVNTQRAAVVFDP
YVATNFLGVIAPALAADAVQKGKSLFRGRVGQQVAAPVINLIDDGCRPDGIASSPFDGEGVPTEHTVLIEKGVLRCFLHN
TYTAARDGVRSTGNGARGSFKTTPEVGTTNFYIEAGSRSPEEIIKEIPKGLYVTEVMGMHTANPISGDFSVGATGIWIEK
GELTTPVRGVAIAGNIIGLLEAIDAVANDLTFFGATGAPTIRIASMTISG
>Mature_450_residues
MDYQELEKKYLDLAGQVVEKAAKRGVLAEAYLTAGEELSIEVRDQQVEALTTARDQGLGLRVIRDHRVGFAFTTDFSPAA
LDACIEQALANARMATPDEHNCLPARYPGYPALDLWDPEITATPLEKKIELAKEIERQARAYDPRVKITESCSYNDSRYL
VALANSQGITAAYHAANCGASTFVVAVENGESQTGFGLAYGLKFKNIDPAKVGREGASKAVRMLGAKRVNTQRAAVVFDP
YVATNFLGVIAPALAADAVQKGKSLFRGRVGQQVAAPVINLIDDGCRPDGIASSPFDGEGVPTEHTVLIEKGVLRCFLHN
TYTAARDGVRSTGNGARGSFKTTPEVGTTNFYIEAGSRSPEEIIKEIPKGLYVTEVMGMHTANPISGDFSVGATGIWIEK
GELTTPVRGVAIAGNIIGLLEAIDAVANDLTFFGATGAPTIRIASMTISG

Specific function: May facilitate the secretion of the peptide antibiotic microcin B17 (MccB17) by completing its maturation. Suppresses the inhibitory activity of the carbon storage regulator (CsrA) [H]

COG id: COG0312

COG function: function code R; Predicted Zn-dependent proteases and their inactivated homologs

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase U62 family [H]

Homologues:

Organism=Escherichia coli, GI1790682, Length=451, Percent_Identity=30.1552106430155, Blast_Score=191, Evalue=7e-50,
Organism=Escherichia coli, GI1789640, Length=469, Percent_Identity=26.0127931769723, Blast_Score=138, Evalue=8e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002510 [H]

Pfam domain/function: PF01523 PmbA_TldD [H]

EC number: NA

Molecular weight: Translated: 48032; Mature: 48032

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: PS00136 SUBTILASE_ASP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYQELEKKYLDLAGQVVEKAAKRGVLAEAYLTAGEELSIEVRDQQVEALTTARDQGLGL
CCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCE
RVIRDHRVGFAFTTDFSPAALDACIEQALANARMATPDEHNCLPARYPGYPALDLWDPEI
EEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TATPLEKKIELAKEIERQARAYDPRVKITESCSYNDSRYLVALANSQGITAAYHAANCGA
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCC
STFVVAVENGESQTGFGLAYGLKFKNIDPAKVGREGASKAVRMLGAKRVNTQRAAVVFDP
CEEEEEEECCCCCCCCEEEEEEEECCCCHHHHCHHHHHHHHHHHHHHHCCCCCEEEEECC
YVATNFLGVIAPALAADAVQKGKSLFRGRVGQQVAAPVINLIDDGCRPDGIASSPFDGEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VPTEHTVLIEKGVLRCFLHNTYTAARDGVRSTGNGARGSFKTTPEVGTTNFYIEAGSRSP
CCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
EEIIKEIPKGLYVTEVMGMHTANPISGDFSVGATGIWIEKGELTTPVRGVAIAGNIIGLL
HHHHHHCCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHH
EAIDAVANDLTFFGATGAPTIRIASMTISG
HHHHHHHHCCEEEECCCCCEEEEEEEEECC
>Mature Secondary Structure
MDYQELEKKYLDLAGQVVEKAAKRGVLAEAYLTAGEELSIEVRDQQVEALTTARDQGLGL
CCHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCCEEEEEECHHHHHHHHHHHHCCCCE
RVIRDHRVGFAFTTDFSPAALDACIEQALANARMATPDEHNCLPARYPGYPALDLWDPEI
EEEEECCCEEEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
TATPLEKKIELAKEIERQARAYDPRVKITESCSYNDSRYLVALANSQGITAAYHAANCGA
CCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEEEEECCCCCEEEEECCCCCC
STFVVAVENGESQTGFGLAYGLKFKNIDPAKVGREGASKAVRMLGAKRVNTQRAAVVFDP
CEEEEEEECCCCCCCCEEEEEEEECCCCHHHHCHHHHHHHHHHHHHHHCCCCCEEEEECC
YVATNFLGVIAPALAADAVQKGKSLFRGRVGQQVAAPVINLIDDGCRPDGIASSPFDGEG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
VPTEHTVLIEKGVLRCFLHNTYTAARDGVRSTGNGARGSFKTTPEVGTTNFYIEAGSRSP
CCCCEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCCH
EEIIKEIPKGLYVTEVMGMHTANPISGDFSVGATGIWIEKGELTTPVRGVAIAGNIIGLL
HHHHHHCCCCEEEHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCCCCCCEEEHHHHHHHH
EAIDAVANDLTFFGATGAPTIRIASMTISG
HHHHHHHHCCEEEECCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]