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The map label for this gene is dacF [H]

Identifier: 83590342

GI number: 83590342

Start: 1544289

End: 1545527

Strand: Reverse

Name: dacF [H]

Synonym: Moth_1499

Alternate gene names: 83590342

Gene position: 1545527-1544289 (Counterclockwise)

Preceding gene: 83590343

Following gene: 83590341

Centisome position: 58.79

GC content: 59.56

Gene sequence:

>1239_bases
GTGCAGAAACTTACAGCGATACTGCTGGCCTTACTCCTGGCCCTCGGAATGGGGCCGGGATTCTTGCTGGCGGCTGATCC
GGCGGATGGGGCCGGAAACGAAGCCGCGCCGGTCACCGGGCAGCCAGGTGAAGTCCAGGCAACGGCAGGCGCGGCAGCCG
GGCCGGGAGATATCAATGCCAAGGGCGCCATCCTCATGGACCCCCAGACAGGGGAGATCCTCTGGGAGAAGAACTCCCAT
GCCCATTATTATCCGGCCAGCATGACCAAACTCATGACCATGGTCGTGGCTATGGACATGGTCGCCAACGGTCAGGCCAC
CCTGGATGAACCGGTCCAGGTGAGCGAACGGGCCGAAAGCTTTGGTGGGTCGGAGGTCTTCCTGGCTGTAGGCGAAACCT
TTCCCCTGGAGCAGATGCTCATCGCTATTGCCGTGGCCTCGGCCAACGACGCGGCCGTAGCGGTGGCCGAGCATCTGGCG
GGTTCGGAGGAAGCCTTCGTGGCCATGATGAACGCCAAGGCCAAGGAACTGGGCCTCAAGGATACCCACTTCGCCAACTG
TCACGGCCTCCATGACGAACAGAATTATACCTCAGCCTATGATATGGCCGTAATTGCCCGTTACGCCCTGAAGTACCCCA
AAATCCGCGAGTGGACCTCCATTAAGCGCTATACCCTGCGTAAAGATCCCCTGACCATCCTGGATACCACCAACAAGATG
CTCTATTGGTACCCGGGAACGGACGGGTTTAAGACCGGCTTCACCGATGCTGCCGGCTTGAACCTTGTTTCAACGGTGGA
GAGGGACGGTTTGCGGTTGGTCGCCGTTGTCATGGGCGTCGAGACGCCCCAGGGGCATTTTACCGAATCTATGAAGCTGT
ACAACTGGGCCTTTAAACAGTGGGCCTTCAAGGAGTTTTACGGCCCGGGCCAGGTAGTGGCCAGCATCCCGGTGGGCAAG
GGCCAGGTGGAGCAGGTAAAGATTGTTACGACCGGGAAGGTCGGTGCCCGGATAAGCCGCCTCCGGGGTAAGGCTGAGGG
CGTGACAACCAAAGTCGAACTGCCGGGTATTGTCAACGCCCCGGTGAAGGAGGGGCAGGTTGTCGGCCAGGCGCTGGTCC
TAAGGGACGGGCAGGTGATCGATAGAGTACAGCTGGTGACGCAACAAAAGGTGGCAAAAGCCTCCCTGGGCCAGGAGATT
GTCCGGGTCATCCGAGCCGTTTTCACTATTCGACAATAA

Upstream 100 bases:

>100_bases
TTATGCAGGTTATCATCAACTGCCGGGATTCACCCCTCATATTTTTGCCCCCTGGATAATATATGTTTAAAGAGAATAAG
CCAGGCAGGGGGGCGGTGGG

Downstream 100 bases:

>100_bases
ATTTGCCATTATTTAGCAGGAAGAAGACGAGGCCATAGCGAATTAACCCCTTCGGAAACCTTGACGAGGGGGTTTTTAGT
TTGCAGGTATTCTTCAGCAT

Product: Serine-type D-Ala-D-Ala carboxypeptidase

Products: NA

Alternate protein names: DD-carboxypeptidase; DD-peptidase; Penicillin-binding protein dacF; PBP [H]

Number of amino acids: Translated: 412; Mature: 412

Protein sequence:

>412_residues
MQKLTAILLALLLALGMGPGFLLAADPADGAGNEAAPVTGQPGEVQATAGAAAGPGDINAKGAILMDPQTGEILWEKNSH
AHYYPASMTKLMTMVVAMDMVANGQATLDEPVQVSERAESFGGSEVFLAVGETFPLEQMLIAIAVASANDAAVAVAEHLA
GSEEAFVAMMNAKAKELGLKDTHFANCHGLHDEQNYTSAYDMAVIARYALKYPKIREWTSIKRYTLRKDPLTILDTTNKM
LYWYPGTDGFKTGFTDAAGLNLVSTVERDGLRLVAVVMGVETPQGHFTESMKLYNWAFKQWAFKEFYGPGQVVASIPVGK
GQVEQVKIVTTGKVGARISRLRGKAEGVTTKVELPGIVNAPVKEGQVVGQALVLRDGQVIDRVQLVTQQKVAKASLGQEI
VRVIRAVFTIRQ

Sequences:

>Translated_412_residues
MQKLTAILLALLLALGMGPGFLLAADPADGAGNEAAPVTGQPGEVQATAGAAAGPGDINAKGAILMDPQTGEILWEKNSH
AHYYPASMTKLMTMVVAMDMVANGQATLDEPVQVSERAESFGGSEVFLAVGETFPLEQMLIAIAVASANDAAVAVAEHLA
GSEEAFVAMMNAKAKELGLKDTHFANCHGLHDEQNYTSAYDMAVIARYALKYPKIREWTSIKRYTLRKDPLTILDTTNKM
LYWYPGTDGFKTGFTDAAGLNLVSTVERDGLRLVAVVMGVETPQGHFTESMKLYNWAFKQWAFKEFYGPGQVVASIPVGK
GQVEQVKIVTTGKVGARISRLRGKAEGVTTKVELPGIVNAPVKEGQVVGQALVLRDGQVIDRVQLVTQQKVAKASLGQEI
VRVIRAVFTIRQ
>Mature_412_residues
MQKLTAILLALLLALGMGPGFLLAADPADGAGNEAAPVTGQPGEVQATAGAAAGPGDINAKGAILMDPQTGEILWEKNSH
AHYYPASMTKLMTMVVAMDMVANGQATLDEPVQVSERAESFGGSEVFLAVGETFPLEQMLIAIAVASANDAAVAVAEHLA
GSEEAFVAMMNAKAKELGLKDTHFANCHGLHDEQNYTSAYDMAVIARYALKYPKIREWTSIKRYTLRKDPLTILDTTNKM
LYWYPGTDGFKTGFTDAAGLNLVSTVERDGLRLVAVVMGVETPQGHFTESMKLYNWAFKQWAFKEFYGPGQVVASIPVGK
GQVEQVKIVTTGKVGARISRLRGKAEGVTTKVELPGIVNAPVKEGQVVGQALVLRDGQVIDRVQLVTQQKVAKASLGQEI
VRVIRAVFTIRQ

Specific function: Removes C-terminal D-alanyl residues from sugar-peptide cell wall precursors [H]

COG id: COG1686

COG function: function code M; D-alanyl-D-alanine carboxypeptidase

Gene ontology:

Cell location: Secreted (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S11 family [H]

Homologues:

Organism=Escherichia coli, GI1786851, Length=363, Percent_Identity=34.1597796143251, Blast_Score=167, Evalue=9e-43,
Organism=Escherichia coli, GI87082022, Length=353, Percent_Identity=32.8611898016997, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1787062, Length=346, Percent_Identity=31.7919075144509, Blast_Score=145, Evalue=5e-36,
Organism=Escherichia coli, GI87082054, Length=247, Percent_Identity=27.9352226720648, Blast_Score=83, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR015956
- InterPro:   IPR018044
- InterPro:   IPR012907
- InterPro:   IPR001967 [H]

Pfam domain/function: PF07943 PBP5_C; PF00768 Peptidase_S11 [H]

EC number: =3.4.16.4 [H]

Molecular weight: Translated: 44190; Mature: 44190

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKLTAILLALLLALGMGPGFLLAADPADGAGNEAAPVTGQPGEVQATAGAAAGPGDINA
CHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
KGAILMDPQTGEILWEKNSHAHYYPASMTKLMTMVVAMDMVANGQATLDEPVQVSERAES
CCEEEECCCCCCEEEECCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
FGGSEVFLAVGETFPLEQMLIAIAVASANDAAVAVAEHLAGSEEAFVAMMNAKAKELGLK
CCCCEEEEEECCCCCHHHHHHHHHEECCCCHHHHHHHHHCCCHHHEEEECCCHHHHCCCC
DTHFANCHGLHDEQNYTSAYDMAVIARYALKYPKIREWTSIKRYTLRKDPLTILDTTNKM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCEEEEECCCCE
LYWYPGTDGFKTGFTDAAGLNLVSTVERDGLRLVAVVMGVETPQGHFTESMKLYNWAFKQ
EEEECCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
WAFKEFYGPGQVVASIPVGKGQVEQVKIVTTGKVGARISRLRGKAEGVTTKVELPGIVNA
HHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCC
PVKEGQVVGQALVLRDGQVIDRVQLVTQQKVAKASLGQEIVRVIRAVFTIRQ
CCCCCHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MQKLTAILLALLLALGMGPGFLLAADPADGAGNEAAPVTGQPGEVQATAGAAAGPGDINA
CHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCC
KGAILMDPQTGEILWEKNSHAHYYPASMTKLMTMVVAMDMVANGQATLDEPVQVSERAES
CCEEEECCCCCCEEEECCCCCEECCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHH
FGGSEVFLAVGETFPLEQMLIAIAVASANDAAVAVAEHLAGSEEAFVAMMNAKAKELGLK
CCCCEEEEEECCCCCHHHHHHHHHEECCCCHHHHHHHHHCCCHHHEEEECCCHHHHCCCC
DTHFANCHGLHDEQNYTSAYDMAVIARYALKYPKIREWTSIKRYTLRKDPLTILDTTNKM
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHEEECCCCEEEEECCCCE
LYWYPGTDGFKTGFTDAAGLNLVSTVERDGLRLVAVVMGVETPQGHFTESMKLYNWAFKQ
EEEECCCCCCCCCCCCCCCCHHHHHHCCCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHH
WAFKEFYGPGQVVASIPVGKGQVEQVKIVTTGKVGARISRLRGKAEGVTTKVELPGIVNA
HHHHHHCCCCCEEEECCCCCCCCEEEEEEECCCHHHHHHHHCCCCCCCEEEEECCCCCCC
PVKEGQVVGQALVLRDGQVIDRVQLVTQQKVAKASLGQEIVRVIRAVFTIRQ
CCCCCHHHCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1629150; 8969508; 9384377 [H]