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The map label for this gene is nirB [C]

Identifier: 83590277

GI number: 83590277

Start: 1474982

End: 1476325

Strand: Reverse

Name: nirB [C]

Synonym: Moth_1431

Alternate gene names: 83590277

Gene position: 1476325-1474982 (Counterclockwise)

Preceding gene: 83590278

Following gene: 83590276

Centisome position: 56.16

GC content: 53.12

Gene sequence:

>1344_bases
TTGAAGGGTACGGATGTACTAGTTATCGGCGGGAGTGCGGCGGGCCTTACGGCAGCTATTACTGCCAAAAGGTATTTTAA
GGACAAAAAGGTAACCGTGGTACGTAAAGAAGAAAAGGTTTTGGTACCCTGCGGTATCCCTTACATTTTCGGTACTGTGG
GGAGCCCCGACAAGAACCTCATCCCGGACGCCACGCTGGTTAATAACGGCATAGAACTGGTGATCGATGAAGTTATAGGA
ATTGACCGCCAAAGCAAGGCTGTTTCTACGGTGAAATCTGGTAGCATCACCTATGAGAAACTCGTCCTGGCTACCGGATC
GTCTCCTGTTGTTCCACCGGTAAAAGGGGCTGACCTGGAAGGCGTTTTCGTCGTCAAAAAAGATGTGGCCTATCTTGAAC
AACTAAAAAAGGCCCTGGACAAAGCAAAAAATTTGGTTATCGTCGGCGGCGGCTTTATTGGCGTTGAGTTTGCCGATGAG
TGCAGGAAGGGGCGAGAATTGAATATTACCATTGTCGAGCTTTTGCCCCATTGCCTGGCCCTGGCATTTGATGATGAGGT
CTGCACCATGGTAGAGGATAAACTCCGCTCTCAAGGGATAAACATTATCACCGGGACAAAAGTAGAAGAATTTCTGGGTA
ACGGTAAAGTAGAACAGGTGAGACTTTCCAATGGCGAAACCCTGCCTGCCGACATAGTGATCTTCGGCATCGGCGTGGCG
CCCAATATTGAACTGGCCCAAATGGCGGGACTGAAAGTTGACCATCGTTTCGGTATCTGGGTTGATGATTATATGCGTAC
CAGTGACCCCAGTATCTTCGCCATTGGTGATTGCGCCAAAAAAAGCTCGTTTTTCGGGCACGGACCGTCCCTCATCAAAC
TGGCTTCCGTAGCCACCCATGAAGCCCGGGTGGCGGGGGCCAACCTGTTCGGCCTCAGGCGGTCCCGGGTGGCGGCAGTA
GGAGTGTTTTCGACAATTGTCGGCGACCTGGCCCTGGGGTCGGTAGGCCTGACGGAAAGGGCGGCCCGGGAAGCGGGTAT
TGAGGTCGTAACTGGTACCGCCAAGGCGCCTGACAGGCATCCAGGGAGTATGCCGGGGACCAGAGAACTGGGCGTAAAGT
TGATCTTTACTAAAGATACGGGTGTAATTTTAGGCGGGCAGGTTTATGGCGGTACTTCGGTGGGCGAGCTGACCAACCTG
CTGGCGGCTGCCGTGCAGCATAAGATGCGGGCCGACGAGCTGGAAACCATGCAGGTAGGGACCCATCCGGCCCTTACCGC
TTCGCCTGTTGCTTATCAAGTAACAAATGCGGCCGAGGACGCCGTGCTTAAGCTAAGAAGCTAA

Upstream 100 bases:

>100_bases
TTTTTCTCTAACTATTATTGACATATGACCTAATAAATACTATACTATTTACAAAACTAAGTTCTAGTATTAACAAAAAA
CATGGAAAGGGGCGGCTGGT

Downstream 100 bases:

>100_bases
TTACCAGCCTATCAGGCTTTCTTACAGCGAGGGTAATTTTTACGCCAGGGGCCCCTCTTGTAAAGGAGGGGCCTTTTTTG
CAAGGCGATATTGACGTATG

Product: FAD-dependent pyridine nucleotide-disulphide oxidoreductase

Products: NA

Alternate protein names: NOXase [H]

Number of amino acids: Translated: 447; Mature: 447

Protein sequence:

>447_residues
MKGTDVLVIGGSAAGLTAAITAKRYFKDKKVTVVRKEEKVLVPCGIPYIFGTVGSPDKNLIPDATLVNNGIELVIDEVIG
IDRQSKAVSTVKSGSITYEKLVLATGSSPVVPPVKGADLEGVFVVKKDVAYLEQLKKALDKAKNLVIVGGGFIGVEFADE
CRKGRELNITIVELLPHCLALAFDDEVCTMVEDKLRSQGINIITGTKVEEFLGNGKVEQVRLSNGETLPADIVIFGIGVA
PNIELAQMAGLKVDHRFGIWVDDYMRTSDPSIFAIGDCAKKSSFFGHGPSLIKLASVATHEARVAGANLFGLRRSRVAAV
GVFSTIVGDLALGSVGLTERAAREAGIEVVTGTAKAPDRHPGSMPGTRELGVKLIFTKDTGVILGGQVYGGTSVGELTNL
LAAAVQHKMRADELETMQVGTHPALTASPVAYQVTNAAEDAVLKLRS

Sequences:

>Translated_447_residues
MKGTDVLVIGGSAAGLTAAITAKRYFKDKKVTVVRKEEKVLVPCGIPYIFGTVGSPDKNLIPDATLVNNGIELVIDEVIG
IDRQSKAVSTVKSGSITYEKLVLATGSSPVVPPVKGADLEGVFVVKKDVAYLEQLKKALDKAKNLVIVGGGFIGVEFADE
CRKGRELNITIVELLPHCLALAFDDEVCTMVEDKLRSQGINIITGTKVEEFLGNGKVEQVRLSNGETLPADIVIFGIGVA
PNIELAQMAGLKVDHRFGIWVDDYMRTSDPSIFAIGDCAKKSSFFGHGPSLIKLASVATHEARVAGANLFGLRRSRVAAV
GVFSTIVGDLALGSVGLTERAAREAGIEVVTGTAKAPDRHPGSMPGTRELGVKLIFTKDTGVILGGQVYGGTSVGELTNL
LAAAVQHKMRADELETMQVGTHPALTASPVAYQVTNAAEDAVLKLRS
>Mature_447_residues
MKGTDVLVIGGSAAGLTAAITAKRYFKDKKVTVVRKEEKVLVPCGIPYIFGTVGSPDKNLIPDATLVNNGIELVIDEVIG
IDRQSKAVSTVKSGSITYEKLVLATGSSPVVPPVKGADLEGVFVVKKDVAYLEQLKKALDKAKNLVIVGGGFIGVEFADE
CRKGRELNITIVELLPHCLALAFDDEVCTMVEDKLRSQGINIITGTKVEEFLGNGKVEQVRLSNGETLPADIVIFGIGVA
PNIELAQMAGLKVDHRFGIWVDDYMRTSDPSIFAIGDCAKKSSFFGHGPSLIKLASVATHEARVAGANLFGLRRSRVAAV
GVFSTIVGDLALGSVGLTERAAREAGIEVVTGTAKAPDRHPGSMPGTRELGVKLIFTKDTGVILGGQVYGGTSVGELTNL
LAAAVQHKMRADELETMQVGTHPALTASPVAYQVTNAAEDAVLKLRS

Specific function: Catalyzes the four-electron reduction of molecular oxygen to water [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-III pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI226437568, Length=315, Percent_Identity=26.984126984127, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI65787454, Length=315, Percent_Identity=26.984126984127, Blast_Score=99, Evalue=6e-21,
Organism=Homo sapiens, GI21389617, Length=315, Percent_Identity=26.984126984127, Blast_Score=99, Evalue=7e-21,
Organism=Homo sapiens, GI22202629, Length=292, Percent_Identity=24.3150684931507, Blast_Score=88, Evalue=1e-17,
Organism=Homo sapiens, GI4757732, Length=292, Percent_Identity=24.3150684931507, Blast_Score=88, Evalue=2e-17,
Organism=Escherichia coli, GI1789765, Length=369, Percent_Identity=28.1842818428184, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1789065, Length=204, Percent_Identity=32.3529411764706, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1788892, Length=199, Percent_Identity=32.1608040201005, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI1786307, Length=346, Percent_Identity=27.4566473988439, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787352, Length=254, Percent_Identity=26.7716535433071, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI87082354, Length=298, Percent_Identity=24.1610738255034, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17559934, Length=212, Percent_Identity=31.1320754716981, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17557007, Length=348, Percent_Identity=25, Blast_Score=70, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24585130, Length=338, Percent_Identity=21.8934911242604, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI281359715, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI281359713, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639250, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI18543267, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639252, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24639257, Length=251, Percent_Identity=26.6932270916335, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI28573993, Length=291, Percent_Identity=24.0549828178694, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24581020, Length=291, Percent_Identity=24.0549828178694, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR004099
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.6.99.3 [H]

Molecular weight: Translated: 47188; Mature: 47188

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGTDVLVIGGSAAGLTAAITAKRYFKDKKVTVVRKEEKVLVPCGIPYIFGTVGSPDKNL
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHEECCCCCCCCCC
IPDATLVNNGIELVIDEVIGIDRQSKAVSTVKSGSITYEKLVLATGSSPVVPPVKGADLE
CCCHHHHCCCHHEEEHHHHCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCC
GVFVVKKDVAYLEQLKKALDKAKNLVIVGGGFIGVEFADECRKGRELNITIVELLPHCLA
EEEEEEHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEHHHHHCCCEEEEEHHHHHHHHHH
LAFDDEVCTMVEDKLRSQGINIITGTKVEEFLGNGKVEQVRLSNGETLPADIVIFGIGVA
HHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCC
PNIELAQMAGLKVDHRFGIWVDDYMRTSDPSIFAIGDCAKKSSFFGHGPSLIKLASVATH
CCCHHHHHCCCEEECEECCEEHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
EARVAGANLFGLRRSRVAAVGVFSTIVGDLALGSVGLTERAAREAGIEVVTGTAKAPDRH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCCCCCCCC
PGSMPGTRELGVKLIFTKDTGVILGGQVYGGTSVGELTNLLAAAVQHKMRADELETMQVG
CCCCCCCHHCCEEEEEEECCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
THPALTASPVAYQVTNAAEDAVLKLRS
CCCCCCCCCCEEEECCCCCHHHEEECC
>Mature Secondary Structure
MKGTDVLVIGGSAAGLTAAITAKRYFKDKKVTVVRKEEKVLVPCGIPYIFGTVGSPDKNL
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCEEEEEECCCEEEECCCHHEECCCCCCCCCC
IPDATLVNNGIELVIDEVIGIDRQSKAVSTVKSGSITYEKLVLATGSSPVVPPVKGADLE
CCCHHHHCCCHHEEEHHHHCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCCCCCCCCCCC
GVFVVKKDVAYLEQLKKALDKAKNLVIVGGGFIGVEFADECRKGRELNITIVELLPHCLA
EEEEEEHHHHHHHHHHHHHHHHCCEEEECCCEEEEEEHHHHHCCCEEEEEHHHHHHHHHH
LAFDDEVCTMVEDKLRSQGINIITGTKVEEFLGNGKVEQVRLSNGETLPADIVIFGIGVA
HHCCCHHHHHHHHHHHHCCCEEEECCCHHHHHCCCCEEEEEECCCCCCCCEEEEEEECCC
PNIELAQMAGLKVDHRFGIWVDDYMRTSDPSIFAIGDCAKKSSFFGHGPSLIKLASVATH
CCCHHHHHCCCEEECEECCEEHHHHCCCCCCEEEEECCCCCCCCCCCCCHHHHHHHHHHH
EARVAGANLFGLRRSRVAAVGVFSTIVGDLALGSVGLTERAAREAGIEVVTGTAKAPDRH
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEECCCCCCCCC
PGSMPGTRELGVKLIFTKDTGVILGGQVYGGTSVGELTNLLAAAVQHKMRADELETMQVG
CCCCCCCHHCCEEEEEEECCCEEECCEEECCCCHHHHHHHHHHHHHHHHHHHHHHEEECC
THPALTASPVAYQVTNAAEDAVLKLRS
CCCCCCCCCCEEEECCCCCHHHEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]