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The map label for this gene is otsB [H]

Identifier: 83593884

GI number: 83593884

Start: 2964879

End: 2965772

Strand: Direct

Name: otsB [H]

Synonym: Rru_A2549

Alternate gene names: 83593884

Gene position: 2964879-2965772 (Clockwise)

Preceding gene: 83593883

Following gene: 83593887

Centisome position: 68.11

GC content: 70.13

Gene sequence:

>894_bases
ATGACCGCCTCGTCGCTTCGCTCGCCCCAGGCGCCGAAAAATCAGGCCAAGCCGATCGAGCAGCTTATCCCCATCCTCGA
CGAGACCGCCTCGTTCCTGGCGGCGCTCAAGGGGCGCACGCCGGTGGTCTTCCTTGATTTCGACGGCACCCTGGCGCCGA
TCGCCGAACGCCCCGAGGCGGTGGTCTTCTCGGCCGCGCGCCGCGAGGCGGTGCGCAAGCTGGCGGGCAAACTGCCCGTC
GCCATCGTCAGCGGCCGCGACCGCGCCGATGTCGAAAAGCAGGTCGGCCTGCCCGGTCTGACCTATGCCGGCGGTCACGG
CTTCGATATTCGCCTCGCCCCGTCGGGCAGTGCCAGCGAGGTTGCGCCCGACGATGCCGATCCGCTGGCGGCCGAGTTGG
ATGGTGGGTTGGCCGGGCTGGTCGAACGCCTTGACGCCATGGAGGCCGCCCTTCACGCCGGGCTCGACGGCATCGCCGGG
GCCTTGATCGAACGCAAGCGCTTCTCGGTCGCCGCCCATGACCGCATGGTCGCCCCGGCCGACCGCCCGGCCTTCGCCGC
CGCCCTGGAGGCGGCGCGCCGCGATCTGCGCGGACTGCGCGAGAAGGCCGGCAAGCGGCTGGTCGAATTCCTGCCCGATA
TCGATTGGGACAAGGGCAAGGCGGTGCTTCACCTGCGCCGCGCCCTGGGGGTGGATGGGGAGGGTTATGTCGCGGTGTTC
TTCGGCGACGACCTGACCGACGAGGACGCCTTCCGCGTTCTGCCCGAGATCGATGGCATCGGCGTTCTGGTCGCCGGCGC
CGACGAGGACGGCCGGGGCAGAACCAGCCACGCCTTCTTCCGCGTCGCCGATCCCGATGGGGTTTGCCGCCTGCTCAACG
ACATGGTGGCCTGA

Upstream 100 bases:

>100_bases
CCGGTTGTCTCCCCCCTGGCGCAGCGAACGCTGAGTCCGCGCGTTGCCTTGCCGCAGCCCGGGCCTTCGGATGCTTCTTC
CGCGCAGAAAGGGATTGTCC

Downstream 100 bases:

>100_bases
CGCCGGCCTTCCCCCCCTCACACCACCCCTTGGGCGCGCAGATCGGCGATTTCGGCCTCGCTCAGCCCCAGCTCACCCAG
AATCCGCCCGCTATCGGCGC

Product: HAD family hydrolase

Products: NA

Alternate protein names: TPP; Trehalose-6-phosphate phosphatase [H]

Number of amino acids: Translated: 297; Mature: 296

Protein sequence:

>297_residues
MTASSLRSPQAPKNQAKPIEQLIPILDETASFLAALKGRTPVVFLDFDGTLAPIAERPEAVVFSAARREAVRKLAGKLPV
AIVSGRDRADVEKQVGLPGLTYAGGHGFDIRLAPSGSASEVAPDDADPLAAELDGGLAGLVERLDAMEAALHAGLDGIAG
ALIERKRFSVAAHDRMVAPADRPAFAAALEAARRDLRGLREKAGKRLVEFLPDIDWDKGKAVLHLRRALGVDGEGYVAVF
FGDDLTDEDAFRVLPEIDGIGVLVAGADEDGRGRTSHAFFRVADPDGVCRLLNDMVA

Sequences:

>Translated_297_residues
MTASSLRSPQAPKNQAKPIEQLIPILDETASFLAALKGRTPVVFLDFDGTLAPIAERPEAVVFSAARREAVRKLAGKLPV
AIVSGRDRADVEKQVGLPGLTYAGGHGFDIRLAPSGSASEVAPDDADPLAAELDGGLAGLVERLDAMEAALHAGLDGIAG
ALIERKRFSVAAHDRMVAPADRPAFAAALEAARRDLRGLREKAGKRLVEFLPDIDWDKGKAVLHLRRALGVDGEGYVAVF
FGDDLTDEDAFRVLPEIDGIGVLVAGADEDGRGRTSHAFFRVADPDGVCRLLNDMVA
>Mature_296_residues
TASSLRSPQAPKNQAKPIEQLIPILDETASFLAALKGRTPVVFLDFDGTLAPIAERPEAVVFSAARREAVRKLAGKLPVA
IVSGRDRADVEKQVGLPGLTYAGGHGFDIRLAPSGSASEVAPDDADPLAAELDGGLAGLVERLDAMEAALHAGLDGIAGA
LIERKRFSVAAHDRMVAPADRPAFAAALEAARRDLRGLREKAGKRLVEFLPDIDWDKGKAVLHLRRALGVDGEGYVAVFF
GDDLTDEDAFRVLPEIDGIGVLVAGADEDGRGRTSHAFFRVADPDGVCRLLNDMVA

Specific function: Removes the phosphate from trehalose 6-phosphate to produce free trehalose [H]

COG id: COG1877

COG function: function code G; Trehalose-6-phosphatase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trehalose phosphatase family [H]

Homologues:

Organism=Drosophila melanogaster, GI221473368, Length=254, Percent_Identity=33.4645669291339, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI20129307, Length=254, Percent_Identity=33.4645669291339, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24582490, Length=254, Percent_Identity=33.4645669291339, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI19920676, Length=280, Percent_Identity=30.3571428571429, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI20129309, Length=255, Percent_Identity=28.6274509803922, Blast_Score=88, Evalue=6e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR006379
- InterPro:   IPR003337 [H]

Pfam domain/function: PF02358 Trehalose_PPase [H]

EC number: =3.1.3.12 [H]

Molecular weight: Translated: 31443; Mature: 31312

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTASSLRSPQAPKNQAKPIEQLIPILDETASFLAALKGRTPVVFLDFDGTLAPIAERPEA
CCCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHCCCHH
VVFSAARREAVRKLAGKLPVAIVSGRDRADVEKQVGLPGLTYAGGHGFDIRLAPSGSASE
HHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCC
VAPDDADPLAAELDGGLAGLVERLDAMEAALHAGLDGIAGALIERKRFSVAAHDRMVAPA
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEECCC
DRPAFAAALEAARRDLRGLREKAGKRLVEFLPDIDWDKGKAVLHLRRALGVDGEGYVAVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
FGDDLTDEDAFRVLPEIDGIGVLVAGADEDGRGRTSHAFFRVADPDGVCRLLNDMVA
ECCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
TASSLRSPQAPKNQAKPIEQLIPILDETASFLAALKGRTPVVFLDFDGTLAPIAERPEA
CCCCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHCCCHH
VVFSAARREAVRKLAGKLPVAIVSGRDRADVEKQVGLPGLTYAGGHGFDIRLAPSGSASE
HHHHHHHHHHHHHHHCCCCEEEECCCCHHHHHHHCCCCCCEECCCCCEEEEECCCCCCCC
VAPDDADPLAAELDGGLAGLVERLDAMEAALHAGLDGIAGALIERKRFSVAAHDRMVAPA
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCEECCC
DRPAFAAALEAARRDLRGLREKAGKRLVEFLPDIDWDKGKAVLHLRRALGVDGEGYVAVF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCCEEEEE
FGDDLTDEDAFRVLPEIDGIGVLVAGADEDGRGRTSHAFFRVADPDGVCRLLNDMVA
ECCCCCCCHHHHHCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]