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The map label for this gene is pleD [H]

Identifier: 83593846

GI number: 83593846

Start: 2918246

End: 2920306

Strand: Direct

Name: pleD [H]

Synonym: Rru_A2511

Alternate gene names: 83593846

Gene position: 2918246-2920306 (Clockwise)

Preceding gene: 83593845

Following gene: 83593850

Centisome position: 67.04

GC content: 65.16

Gene sequence:

>2061_bases
ATGCAAGCCTTTCCGGCATATCCTTCCCGTCGAAAATGGCGAAGGCGGCTTTTCCTTCTCGGCGTCATCGGCCTGCTTGC
CTGCGTGAACGGGGCACTCGCCCTGGCCCAAGGCGCTCCTCCTCCCCAGGATGAGCCCCTCCACCTGCGACTGAAATGGA
AGCACCAGTTCCAATTCGCCGGCTATTACGCCGCCCTTGAAAAAGGTTTCTATGCCGGCGAAGGGCTGAGCGTCGTTCTT
GAAGAAGCCTCCGACAGCGAGGACGCGGTAACGGCGGTGCTCGCCGGCAAGGCCCAGTATGGCATCGATTCGACCGAGCT
TCTGGTCGCCTATGGCCGGGGCGAGCCGGTTGTCGCCCTGGCCGCCCTCTTCCAACATTCGCCCGAGGTGCTGCTGGCGC
GAACCGACTCGGGAATAACCTTGCTTCACGATCTGATCGGCCGGCGGATCTCCCTGCCGCCCGCGTCGCTGTCCCTGCTG
GCCTATCTTGCCGCCGAGGGTATCGGCGCCAACCAGATGACGATCGTCAGGAACCCGTTTTCGCCCGATCCCCTGCTCCA
GGGCGAGGTCGACGCCATCGGCGCCTATTCCAGCGACGAACCGTATTCGCTGCGCCGCCAGGGCATCGATTTCCTGATGT
TCTCGCCGCGTTCGGCCGGCATCGATCTCTACGGGGATATCTTGTTCACCACGGCCGCCGAGCTGCGCAGCCATCCCGAC
CGGGTGCGGGCCTTCCGCCGGGCGAGCCTTCGGGGCTGGGAATACGCCCTGGCCCACCCCCAGGAAATCGCCCGGCTTAT
CGCCGATCGCTACCCCGGCCAGCGGACCATCGATCATCTTCTGTTCGAGGCGGCGGCCATGCGCCAGCTTATCGCCGCCG
ATCTGATCGAGCCGGGTTATATGACCCGGGGACGCTGGCAGGCGATCGCCGATCTTTACGCCGGCTTTGGCATGATCGGC
CGCGATCTCGACCTGGGTCCCTTCCTCTATGGCCTTGCCGAGACCGACAGAACGCCAAGGATCCCCCCGGGCCTGCTTCC
GGCCTCGGGCCTTATCGGCGCCTTCGGTCTGGTCGCGGTGTTCGGCCTGTTGCGCTATCGCAAGATCAAACGGGCCCTGA
CCCGGGAGATCGGCGAGCGGCGCATCGTGGAAAGCCACCTGCGCCAAAGCGAAACCCGCTATCGGATGATGATCGAGGCC
GCGCCCTTTGGCGTGCTCATCAGTTCGCTGAGCACCGATCACATCGTGCAGATCAACGCCCGGGCCGAGGATCAGCTACA
GGCCATGCGCCACTGGGTGATCGACAAACCCTTTGCCGATCTTTTCGTCGATCCCGCCGATCACCGACGCCTGCTTGACA
CCTTGAACAAGACCGGAAGCATCGCCAATTTCGTCGCCCTGTTGCGCACCGCCACCGACAATGACTTCTGGGCGGAGCTC
TCCGCCGCGGTGATCGATCTCGACCGCTCGCCCGCCGTCTTCGCCAGCTTCAACGATATTTCCCAGACCAAGGCGCTTGA
ACGGGAATTACGCGCCCTCGCCGAGCGCGACTCGCTGACCGGTCTGGCCAACCGCCTGCGGGTGATGGACGCGCTGCGCT
CTGAATTCGACCGCGTCACCCGCTATGGCGGGGCCTTATCGGTGATGATGCTGGACCTTGATCGCTTCAAGGTGCTCAAC
GACCGCCATGGTCACGTCTGCGGCGACGAGGCGTTGCGCCATTTCGCCGCCTCCGCCCTGGCGACCCTGCGCACCAACGA
CCTGCTTGGCCGCTCGGGTGGCGAAGAGTTCATCGTGCTGTTGCCCGGCGCGGCCTTGGGCGCAGCCAAGCTGATCGGCG
AGCGCCTGCGCGCCGCCGTCCAGGATAAGCCGCTGTCTTGGCAAGGCCGATCGGTGGCCCTTTCGGTCAGCATCGGCCTT
GCCAGCCGCCAGATCGGCGATACCCCCGACAGCCTGCTCGGCCGGGCCGATCAGGCCCTCCACCTAGCCAAGGCCAACGG
CCGCAACCGCCTGGAAACCGCCGATGGCCCACCGACGACGGCGACGGTCATTCCGCTGTGA

Upstream 100 bases:

>100_bases
CCCGCCGCCGACCAGAGAGTCCGGCCCTTCGCCGCCCTTGTCTTGGTGATAGCGGGGATGTCATGCTCTCTACGCCTTTT
TGAGTGTAGAGGTTGTCTTC

Downstream 100 bases:

>100_bases
AGGCGATGCCGGCCGACAGGCTTACAGCAGGTGCTGGCCGCCGGTCACATAGATCTCCGTGCCGGTGACATAGCCAAATT
CCTCGGTGCACAGGCGATAG

Product: diguanylate cyclase

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 686; Mature: 686

Protein sequence:

>686_residues
MQAFPAYPSRRKWRRRLFLLGVIGLLACVNGALALAQGAPPPQDEPLHLRLKWKHQFQFAGYYAALEKGFYAGEGLSVVL
EEASDSEDAVTAVLAGKAQYGIDSTELLVAYGRGEPVVALAALFQHSPEVLLARTDSGITLLHDLIGRRISLPPASLSLL
AYLAAEGIGANQMTIVRNPFSPDPLLQGEVDAIGAYSSDEPYSLRRQGIDFLMFSPRSAGIDLYGDILFTTAAELRSHPD
RVRAFRRASLRGWEYALAHPQEIARLIADRYPGQRTIDHLLFEAAAMRQLIAADLIEPGYMTRGRWQAIADLYAGFGMIG
RDLDLGPFLYGLAETDRTPRIPPGLLPASGLIGAFGLVAVFGLLRYRKIKRALTREIGERRIVESHLRQSETRYRMMIEA
APFGVLISSLSTDHIVQINARAEDQLQAMRHWVIDKPFADLFVDPADHRRLLDTLNKTGSIANFVALLRTATDNDFWAEL
SAAVIDLDRSPAVFASFNDISQTKALERELRALAERDSLTGLANRLRVMDALRSEFDRVTRYGGALSVMMLDLDRFKVLN
DRHGHVCGDEALRHFAASALATLRTNDLLGRSGGEEFIVLLPGAALGAAKLIGERLRAAVQDKPLSWQGRSVALSVSIGL
ASRQIGDTPDSLLGRADQALHLAKANGRNRLETADGPPTTATVIPL

Sequences:

>Translated_686_residues
MQAFPAYPSRRKWRRRLFLLGVIGLLACVNGALALAQGAPPPQDEPLHLRLKWKHQFQFAGYYAALEKGFYAGEGLSVVL
EEASDSEDAVTAVLAGKAQYGIDSTELLVAYGRGEPVVALAALFQHSPEVLLARTDSGITLLHDLIGRRISLPPASLSLL
AYLAAEGIGANQMTIVRNPFSPDPLLQGEVDAIGAYSSDEPYSLRRQGIDFLMFSPRSAGIDLYGDILFTTAAELRSHPD
RVRAFRRASLRGWEYALAHPQEIARLIADRYPGQRTIDHLLFEAAAMRQLIAADLIEPGYMTRGRWQAIADLYAGFGMIG
RDLDLGPFLYGLAETDRTPRIPPGLLPASGLIGAFGLVAVFGLLRYRKIKRALTREIGERRIVESHLRQSETRYRMMIEA
APFGVLISSLSTDHIVQINARAEDQLQAMRHWVIDKPFADLFVDPADHRRLLDTLNKTGSIANFVALLRTATDNDFWAEL
SAAVIDLDRSPAVFASFNDISQTKALERELRALAERDSLTGLANRLRVMDALRSEFDRVTRYGGALSVMMLDLDRFKVLN
DRHGHVCGDEALRHFAASALATLRTNDLLGRSGGEEFIVLLPGAALGAAKLIGERLRAAVQDKPLSWQGRSVALSVSIGL
ASRQIGDTPDSLLGRADQALHLAKANGRNRLETADGPPTTATVIPL
>Mature_686_residues
MQAFPAYPSRRKWRRRLFLLGVIGLLACVNGALALAQGAPPPQDEPLHLRLKWKHQFQFAGYYAALEKGFYAGEGLSVVL
EEASDSEDAVTAVLAGKAQYGIDSTELLVAYGRGEPVVALAALFQHSPEVLLARTDSGITLLHDLIGRRISLPPASLSLL
AYLAAEGIGANQMTIVRNPFSPDPLLQGEVDAIGAYSSDEPYSLRRQGIDFLMFSPRSAGIDLYGDILFTTAAELRSHPD
RVRAFRRASLRGWEYALAHPQEIARLIADRYPGQRTIDHLLFEAAAMRQLIAADLIEPGYMTRGRWQAIADLYAGFGMIG
RDLDLGPFLYGLAETDRTPRIPPGLLPASGLIGAFGLVAVFGLLRYRKIKRALTREIGERRIVESHLRQSETRYRMMIEA
APFGVLISSLSTDHIVQINARAEDQLQAMRHWVIDKPFADLFVDPADHRRLLDTLNKTGSIANFVALLRTATDNDFWAEL
SAAVIDLDRSPAVFASFNDISQTKALERELRALAERDSLTGLANRLRVMDALRSEFDRVTRYGGALSVMMLDLDRFKVLN
DRHGHVCGDEALRHFAASALATLRTNDLLGRSGGEEFIVLLPGAALGAAKLIGERLRAAVQDKPLSWQGRSVALSVSIGL
ASRQIGDTPDSLLGRADQALHLAKANGRNRLETADGPPTTATVIPL

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1787802, Length=186, Percent_Identity=33.8709677419355, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI87082007, Length=180, Percent_Identity=37.7777777777778, Blast_Score=108, Evalue=1e-24,
Organism=Escherichia coli, GI145693134, Length=181, Percent_Identity=37.5690607734807, Blast_Score=107, Evalue=3e-24,
Organism=Escherichia coli, GI87081881, Length=182, Percent_Identity=36.8131868131868, Blast_Score=105, Evalue=9e-24,
Organism=Escherichia coli, GI1788381, Length=178, Percent_Identity=34.8314606741573, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI1786584, Length=227, Percent_Identity=33.0396475770925, Blast_Score=100, Evalue=5e-22,
Organism=Escherichia coli, GI1787816, Length=206, Percent_Identity=34.4660194174757, Blast_Score=89, Evalue=8e-19,
Organism=Escherichia coli, GI1787541, Length=182, Percent_Identity=31.8681318681319, Blast_Score=87, Evalue=3e-18,
Organism=Escherichia coli, GI1787262, Length=172, Percent_Identity=31.9767441860465, Blast_Score=85, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=154, Percent_Identity=26.6233766233766, Blast_Score=63, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 75220; Mature: 75220

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAFPAYPSRRKWRRRLFLLGVIGLLACVNGALALAQGAPPPQDEPLHLRLKWKHQFQFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEEECEEHH
GYYAALEKGFYAGEGLSVVLEEASDSEDAVTAVLAGKAQYGIDSTELLVAYGRGEPVVAL
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCHHCCCCCCEEEEEECCCCHHHHH
AALFQHSPEVLLARTDSGITLLHDLIGRRISLPPASLSLLAYLAAEGIGANQMTIVRNPF
HHHHHCCCCEEEEECCCCHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCCCEEEEECCC
SPDPLLQGEVDAIGAYSSDEPYSLRRQGIDFLMFSPRSAGIDLYGDILFTTAAELRSHPD
CCCCCCCCCCHHCCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCHH
RVRAFRRASLRGWEYALAHPQEIARLIADRYPGQRTIDHLLFEAAAMRQLIAADLIEPGY
HHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MTRGRWQAIADLYAGFGMIGRDLDLGPFLYGLAETDRTPRIPPGLLPASGLIGAFGLVAV
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
FGLLRYRKIKRALTREIGERRIVESHLRQSETRYRMMIEAAPFGVLISSLSTDHIVQINA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCEEEEEC
RAEDQLQAMRHWVIDKPFADLFVDPADHRRLLDTLNKTGSIANFVALLRTATDNDFWAEL
CCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
SAAVIDLDRSPAVFASFNDISQTKALERELRALAERDSLTGLANRLRVMDALRSEFDRVT
HHHHEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGGALSVMMLDLDRFKVLNDRHGHVCGDEALRHFAASALATLRTNDLLGRSGGEEFIVL
HHCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LPGAALGAAKLIGERLRAAVQDKPLSWQGRSVALSVSIGLASRQIGDTPDSLLGRADQAL
ECCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHCCCCHHHHHCCHHHHH
HLAKANGRNRLETADGPPTTATVIPL
HHHHCCCCCCCCCCCCCCCCEEEECC
>Mature Secondary Structure
MQAFPAYPSRRKWRRRLFLLGVIGLLACVNGALALAQGAPPPQDEPLHLRLKWKHQFQFA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCCEEEEEEEEECEEHH
GYYAALEKGFYAGEGLSVVLEEASDSEDAVTAVLAGKAQYGIDSTELLVAYGRGEPVVAL
HHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHCCCHHCCCCCCEEEEEECCCCHHHHH
AALFQHSPEVLLARTDSGITLLHDLIGRRISLPPASLSLLAYLAAEGIGANQMTIVRNPF
HHHHHCCCCEEEEECCCCHHHHHHHHCCEECCCCHHHHHHHHHHHCCCCCCCEEEEECCC
SPDPLLQGEVDAIGAYSSDEPYSLRRQGIDFLMFSPRSAGIDLYGDILFTTAAELRSHPD
CCCCCCCCCCHHCCCCCCCCCHHHHHCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCCHH
RVRAFRRASLRGWEYALAHPQEIARLIADRYPGQRTIDHLLFEAAAMRQLIAADLIEPGY
HHHHHHHHHCCCCHHHHCCHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
MTRGRWQAIADLYAGFGMIGRDLDLGPFLYGLAETDRTPRIPPGLLPASGLIGAFGLVAV
CCCCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
FGLLRYRKIKRALTREIGERRIVESHLRQSETRYRMMIEAAPFGVLISSLSTDHIVQINA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCHHHHHHHCCCCCEEEEEC
RAEDQLQAMRHWVIDKPFADLFVDPADHRRLLDTLNKTGSIANFVALLRTATDNDFWAEL
CCHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCHHHHHH
SAAVIDLDRSPAVFASFNDISQTKALERELRALAERDSLTGLANRLRVMDALRSEFDRVT
HHHHEECCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RYGGALSVMMLDLDRFKVLNDRHGHVCGDEALRHFAASALATLRTNDLLGRSGGEEFIVL
HHCCCEEEEEEHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEE
LPGAALGAAKLIGERLRAAVQDKPLSWQGRSVALSVSIGLASRQIGDTPDSLLGRADQAL
ECCCHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEECHHHHHCCCCHHHHHCCHHHHH
HLAKANGRNRLETADGPPTTATVIPL
HHHHCCCCCCCCCCCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA