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The map label for this gene is otsA [H]

Identifier: 83593820

GI number: 83593820

Start: 2888640

End: 2890022

Strand: Direct

Name: otsA [H]

Synonym: Rru_A2485

Alternate gene names: 83593820

Gene position: 2888640-2890022 (Clockwise)

Preceding gene: 83593819

Following gene: 83593821

Centisome position: 66.36

GC content: 62.47

Gene sequence:

>1383_bases
GTGGCTCGTCTTATCGTTGTCTCCAACCGCGTGGCGCTTCCCGAGGATCAAGCCGGCCGCGCGGGGGGATTGGCCGTGGC
TTTGCGCGAAGCGCTGGTCTCGTTTGGCGGGATCTGGTTTGGCTGGAGCGGCAAGGTCGCCGCCCAGACATCGGACCGGC
CGCGCTTCATCGAGGAAGGCCCGGTCACCTATGCGGTGCTCGATCTTGGCCGCAAGGATCACGCCCAGTATTACAACGGT
TTCGCCAATCGCACCCTGTGGCCGCTTTTTCATTATCGCATGGGGTTGGCCGAATTCACCCGCCAGGACTACGAAGGCTA
TATGCGGGTCAATACGGTTTTCGCCGAGCAGCTTCAACCGCTGCTGACCGATGATGATCTGATCTGGGTCCATGATTATC
ACCTGATCCCGCTGGGGGGAGAACTGCGCCGCCTGGGGGCGCGCCAGCGCATGGGCATCTTCCTGCACACGCCCTTTCCG
GCGATGGAGGTATTGCTGGCCCTGACCCACCATCGCCGACTGGTCGGTTCGCTGTGCGCCTATGATGTCGTCGGCTTCCA
GACCGAAAACGACCGGCGCTCGTTCTTCGATTACGTCCTGCATGAAGCCGGCGGCTGGATTGGCGAGAACGGCCATGTCA
CCGTCTGGGGGCGCTCGCTGGTCGCCCGGGTTTATCCCATCGGCATCGAGAAAGGCGCCTTCGCCGAGGCCGCCCAGCGC
GCCCAGCGCACGGTCGCCTATCGCCGGCTGCGCGCCAGCCTGAACGGCCGCAAGCTGATGATCGGGGTCGAGCGCCTGGA
TTATTCCAAGGGCCTGCCCGAACGCTTCGAGGCTTTTTCGCGCCTGCTCGAAAAGCATCCCGAGCATCGCGCCAAGGTCA
GTTTCATGCAGGTCGCGCCAATTTCCCGCTCCGAGGTTCCCGAATACCGGCGCCTGCGCGAGGAACTCGAAAGCCTGTCG
GGCCAGATCAATGGCCAGTTCGGCGATTACGATTGGGTGCCAATCCGCTACCTCACCCGCAACTTCGCCAGATCGGTCCT
GGCCGGTTTCTACCGACAAGGGGCCGTCGGCGTTGTCACGCCAATGCGCGATGGTATGAATCTTGTGGCAAAAGAATACG
TTGCCAGCCAGAATCCCCAGGACCCCGGGGTGCTGGTTCTTTCGCGCTTCGCCGGGGCGGCCCGCGAACTGACAACGGCG
CTTCAGGTCAACCCCTTCGACCGCGAGGATATGGCCGATGCCATGGACAGGGCCTTGGGCATGGCTTTGGACGAGCGCAA
GGAGCGTTGGGTTGAAATGATGCGTATGGTCGATGAAAACTCGCTGGAAGCTTGGCGCGAGAAGTATCTGGCCGATCTGA
ACGCGGCGCCGTTCAATGGCTGA

Upstream 100 bases:

>100_bases
ACCCTCCCCGCGCCTTGGCGCCCCGGGCCGCTTTGCGGGAACCCCCTCGCCGGTTGAGCCGTTTTCCGGCGACTCAATCT
TTTAAAGGGGGTGGAATCGC

Downstream 100 bases:

>100_bases
GCCGTTCATTCCCGGATTGATCGCCGATATCGGCGGCACCAACGCCCGCTTCGCCCTGACCACCCCCGAGGGCGGCTGGC
GCGACGAACGGGTTTACCGC

Product: alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 460; Mature: 459

Protein sequence:

>460_residues
MARLIVVSNRVALPEDQAGRAGGLAVALREALVSFGGIWFGWSGKVAAQTSDRPRFIEEGPVTYAVLDLGRKDHAQYYNG
FANRTLWPLFHYRMGLAEFTRQDYEGYMRVNTVFAEQLQPLLTDDDLIWVHDYHLIPLGGELRRLGARQRMGIFLHTPFP
AMEVLLALTHHRRLVGSLCAYDVVGFQTENDRRSFFDYVLHEAGGWIGENGHVTVWGRSLVARVYPIGIEKGAFAEAAQR
AQRTVAYRRLRASLNGRKLMIGVERLDYSKGLPERFEAFSRLLEKHPEHRAKVSFMQVAPISRSEVPEYRRLREELESLS
GQINGQFGDYDWVPIRYLTRNFARSVLAGFYRQGAVGVVTPMRDGMNLVAKEYVASQNPQDPGVLVLSRFAGAARELTTA
LQVNPFDREDMADAMDRALGMALDERKERWVEMMRMVDENSLEAWREKYLADLNAAPFNG

Sequences:

>Translated_460_residues
MARLIVVSNRVALPEDQAGRAGGLAVALREALVSFGGIWFGWSGKVAAQTSDRPRFIEEGPVTYAVLDLGRKDHAQYYNG
FANRTLWPLFHYRMGLAEFTRQDYEGYMRVNTVFAEQLQPLLTDDDLIWVHDYHLIPLGGELRRLGARQRMGIFLHTPFP
AMEVLLALTHHRRLVGSLCAYDVVGFQTENDRRSFFDYVLHEAGGWIGENGHVTVWGRSLVARVYPIGIEKGAFAEAAQR
AQRTVAYRRLRASLNGRKLMIGVERLDYSKGLPERFEAFSRLLEKHPEHRAKVSFMQVAPISRSEVPEYRRLREELESLS
GQINGQFGDYDWVPIRYLTRNFARSVLAGFYRQGAVGVVTPMRDGMNLVAKEYVASQNPQDPGVLVLSRFAGAARELTTA
LQVNPFDREDMADAMDRALGMALDERKERWVEMMRMVDENSLEAWREKYLADLNAAPFNG
>Mature_459_residues
ARLIVVSNRVALPEDQAGRAGGLAVALREALVSFGGIWFGWSGKVAAQTSDRPRFIEEGPVTYAVLDLGRKDHAQYYNGF
ANRTLWPLFHYRMGLAEFTRQDYEGYMRVNTVFAEQLQPLLTDDDLIWVHDYHLIPLGGELRRLGARQRMGIFLHTPFPA
MEVLLALTHHRRLVGSLCAYDVVGFQTENDRRSFFDYVLHEAGGWIGENGHVTVWGRSLVARVYPIGIEKGAFAEAAQRA
QRTVAYRRLRASLNGRKLMIGVERLDYSKGLPERFEAFSRLLEKHPEHRAKVSFMQVAPISRSEVPEYRRLREELESLSG
QINGQFGDYDWVPIRYLTRNFARSVLAGFYRQGAVGVVTPMRDGMNLVAKEYVASQNPQDPGVLVLSRFAGAARELTTAL
QVNPFDREDMADAMDRALGMALDERKERWVEMMRMVDENSLEAWREKYLADLNAAPFNG

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=454, Percent_Identity=48.4581497797357, Blast_Score=445, Evalue=1e-126,
Organism=Caenorhabditis elegans, GI115533560, Length=355, Percent_Identity=31.830985915493, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI115533558, Length=355, Percent_Identity=31.830985915493, Blast_Score=167, Evalue=1e-41,
Organism=Caenorhabditis elegans, GI71987012, Length=416, Percent_Identity=31.0096153846154, Blast_Score=166, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319602, Length=467, Percent_Identity=33.4047109207709, Blast_Score=271, Evalue=1e-73,
Organism=Saccharomyces cerevisiae, GI6320279, Length=422, Percent_Identity=26.0663507109005, Blast_Score=161, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6323537, Length=434, Percent_Identity=26.2672811059908, Blast_Score=155, Evalue=1e-38,
Organism=Saccharomyces cerevisiae, GI6323917, Length=386, Percent_Identity=26.4248704663212, Blast_Score=154, Evalue=4e-38,
Organism=Drosophila melanogaster, GI19920676, Length=488, Percent_Identity=34.6311475409836, Blast_Score=277, Evalue=1e-74,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 52293; Mature: 52162

Theoretical pI: Translated: 8.36; Mature: 8.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARLIVVSNRVALPEDQAGRAGGLAVALREALVSFGGIWFGWSGKVAAQTSDRPRFIEEG
CCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCC
PVTYAVLDLGRKDHAQYYNGFANRTLWPLFHYRMGLAEFTRQDYEGYMRVNTVFAEQLQP
CEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTDDDLIWVHDYHLIPLGGELRRLGARQRMGIFLHTPFPAMEVLLALTHHRRLVGSLCA
HCCCCCEEEEEEEEEEECCHHHHHHCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
YDVVGFQTENDRRSFFDYVLHEAGGWIGENGHVTVWGRSLVARVYPIGIEKGAFAEAAQR
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHH
AQRTVAYRRLRASLNGRKLMIGVERLDYSKGLPERFEAFSRLLEKHPEHRAKVSFMQVAP
HHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECC
ISRSEVPEYRRLREELESLSGQINGQFGDYDWVPIRYLTRNFARSVLAGFYRQGAVGVVT
CCHHCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PMRDGMNLVAKEYVASQNPQDPGVLVLSRFAGAARELTTALQVNPFDREDMADAMDRALG
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
MALDERKERWVEMMRMVDENSLEAWREKYLADLNAAPFNG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ARLIVVSNRVALPEDQAGRAGGLAVALREALVSFGGIWFGWSGKVAAQTSDRPRFIEEG
CEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCCEEECCCCCEEECCCCCCCCCCCC
PVTYAVLDLGRKDHAQYYNGFANRTLWPLFHYRMGLAEFTRQDYEGYMRVNTVFAEQLQP
CEEEEEEECCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LLTDDDLIWVHDYHLIPLGGELRRLGARQRMGIFLHTPFPAMEVLLALTHHRRLVGSLCA
HCCCCCEEEEEEEEEEECCHHHHHHCCHHHCCEEEECCCHHHHHHHHHHHHHHHHHHHHH
YDVVGFQTENDRRSFFDYVLHEAGGWIGENGHVTVWGRSLVARVYPIGIEKGAFAEAAQR
HHHHCCCCCCHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHCCCCCCCHHHHHHHH
AQRTVAYRRLRASLNGRKLMIGVERLDYSKGLPERFEAFSRLLEKHPEHRAKVSFMQVAP
HHHHHHHHHHHHCCCCCEEEEEEEHHCCCCCCHHHHHHHHHHHHHCCCHHHHEEEEEECC
ISRSEVPEYRRLREELESLSGQINGQFGDYDWVPIRYLTRNFARSVLAGFYRQGAVGVVT
CCHHCCHHHHHHHHHHHHHCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEC
PMRDGMNLVAKEYVASQNPQDPGVLVLSRFAGAARELTTALQVNPFDREDMADAMDRALG
CCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHH
MALDERKERWVEMMRMVDENSLEAWREKYLADLNAAPFNG
HHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA