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The map label for this gene is yfkN [H]

Identifier: 83593818

GI number: 83593818

Start: 2885144

End: 2886682

Strand: Direct

Name: yfkN [H]

Synonym: Rru_A2483

Alternate gene names: 83593818

Gene position: 2885144-2886682 (Clockwise)

Preceding gene: 83593817

Following gene: 83593819

Centisome position: 66.28

GC content: 69.07

Gene sequence:

>1539_bases
ATGGCTTTTTCCCGCTGGCTTGTTGGTTTTCTGCTTGCCCTGTCGCTGGTCGCACCGGCGATGGCCGAGGACGGTCGGCC
CGCCGTCACCTTGCGCCTTCTTCATCTCAACGACATCGCCGAAGTCGGCAATGGCGGCGGGTCGGGCGGATTGGCCGAGA
TGAAGACGCTGATCGAGGCCGAGAAGGCCCGCCCCGGCGCCACGGCGGCGCTGGTCACCTTCGGCGGCGATCTGCTCTCG
CCCTCGCTGCTGTCGAGCATCGACAAGGGCGCCCATATGGTCGAGGCGATGGGGCTGCTTGGCGTCGATCTGGCGGTGCC
GGGCAATCATGAATTCGACTTCGGCCCCAAGGTCTTCGCCGAGCGGCTGTCGACCTCGCCCTTCCCTTGGCTGGCCAGCA
ATCTGGACGGCCCGGCGCTGTCGACCGCCGAGGGCGCCCCGATCACGGGGCTGACGTCCACGGCGATCCGCGCCGTCGGT
CCCTATAAGATCGGCTTCCTGGGGCTGCTCACCCCGCAGACCACGGTCTTGTCCTCGCCCGGTCCCGAGGTGCGGTTCGC
CGAGCCGCTGGCGGCGGCGCGCGCCGCCGTGGCCACGCTCAAGGCCGAAGGCGCCGATGTCATCATCGCCCTCACCCACC
AGGGGCAGGCCGAAGACCGCGCCCTGGCCCAGGCCCTGCCCGAGGTGGCGGTGGTGGTGGGGGGACATGACCACGACTCG
CTGTCCTGGCGCGAAAGTACGGGACTGGTGCTCAAGGCCGGGGCCAATGCCCGCTATCTGGGGGTTATCGATCTGCGGAT
CGACGAGCAGATCACCAGCGCCGGCAGCGCGGTGGTGGTTCTGCCCGAATGGTCGTTCCGCGCCAATGTCGGCCTCGCCC
CCTCCCCCGCGGTCGCCGCCCGTCTCGCCGCCTGGGAGGCCCAATTGGCCAGCGATCTTGATCAGCCGCTGGGGGTGACG
ACCACGGAACTCGACAGCCGGCGCTCGGTCGTGCGCGGGGCCGAAAGCACGATGGGCAATCTGGTCACCGACGCCATGCG
CCTGGCCACCGGCGCCCAGGCGGCCCTGACCAATGGCGGGGGCTTGCGCGGCGATCGCCTGTATAACGCCGGAACCGCGT
TGACCCGCCGCGACATCCTGGCCGAACTGCCCTTTGGCAATGTCGCCGTGGTTCTGGATCTGACCGGCGCCCAGGTGAAG
GCCGCCCTGGAGAACGGCGTCTCGCAAATCGCCCAAGGGGCCGGGCGCTTCCCGCAGGTTTCGGGAATGGAGGTCCTGTA
CGATCCCGCCTTGCCGGTCGGCGAGCGCGTCGTCGCCCTGCAAATCGACGGACGCACTGTCGAGCCCGAGGGCCATTACA
CGGTGGCGGTCAATGATTACATCGCGCGCGGCGGTGACGGCTACGACGCCCTTGGCGGCGGGCGGCCGATCATCGATGCC
GCCGCCGGTCGGCTTCTCGTCACCCTTGTCGCCGATCATATCGCCCAAGCCGGCCGGATCGGCGCCCAGATCGAGGGGCG
GCTGGTTTTGCGAAAGTAA

Upstream 100 bases:

>100_bases
GAGGCCGGGCACATCGGCTTCCCTCATGGCGTTTGACACGGTTTCCTTCGCGCGCCTTTGCCCCCACAGTGGGGCTCTCG
TCTAGTCTCGGAGGCGCGTC

Downstream 100 bases:

>100_bases
GCCGCCTTTGGCCTTTGGTTAGATATATCCTAAAGGGGATTGCACGCCGGTATGTGAAGGGCAAAATAGCCCGGAAACCC
ATCCGAAAAGGTCCATTCTG

Product: 5'-nucleotidase

Products: NA

Alternate protein names: 2',3'-cyclic-nucleotide 2'-phosphodiesterase/3'-nucleotidase; 5'-nucleotidase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MAFSRWLVGFLLALSLVAPAMAEDGRPAVTLRLLHLNDIAEVGNGGGSGGLAEMKTLIEAEKARPGATAALVTFGGDLLS
PSLLSSIDKGAHMVEAMGLLGVDLAVPGNHEFDFGPKVFAERLSTSPFPWLASNLDGPALSTAEGAPITGLTSTAIRAVG
PYKIGFLGLLTPQTTVLSSPGPEVRFAEPLAAARAAVATLKAEGADVIIALTHQGQAEDRALAQALPEVAVVVGGHDHDS
LSWRESTGLVLKAGANARYLGVIDLRIDEQITSAGSAVVVLPEWSFRANVGLAPSPAVAARLAAWEAQLASDLDQPLGVT
TTELDSRRSVVRGAESTMGNLVTDAMRLATGAQAALTNGGGLRGDRLYNAGTALTRRDILAELPFGNVAVVLDLTGAQVK
AALENGVSQIAQGAGRFPQVSGMEVLYDPALPVGERVVALQIDGRTVEPEGHYTVAVNDYIARGGDGYDALGGGRPIIDA
AAGRLLVTLVADHIAQAGRIGAQIEGRLVLRK

Sequences:

>Translated_512_residues
MAFSRWLVGFLLALSLVAPAMAEDGRPAVTLRLLHLNDIAEVGNGGGSGGLAEMKTLIEAEKARPGATAALVTFGGDLLS
PSLLSSIDKGAHMVEAMGLLGVDLAVPGNHEFDFGPKVFAERLSTSPFPWLASNLDGPALSTAEGAPITGLTSTAIRAVG
PYKIGFLGLLTPQTTVLSSPGPEVRFAEPLAAARAAVATLKAEGADVIIALTHQGQAEDRALAQALPEVAVVVGGHDHDS
LSWRESTGLVLKAGANARYLGVIDLRIDEQITSAGSAVVVLPEWSFRANVGLAPSPAVAARLAAWEAQLASDLDQPLGVT
TTELDSRRSVVRGAESTMGNLVTDAMRLATGAQAALTNGGGLRGDRLYNAGTALTRRDILAELPFGNVAVVLDLTGAQVK
AALENGVSQIAQGAGRFPQVSGMEVLYDPALPVGERVVALQIDGRTVEPEGHYTVAVNDYIARGGDGYDALGGGRPIIDA
AAGRLLVTLVADHIAQAGRIGAQIEGRLVLRK
>Mature_511_residues
AFSRWLVGFLLALSLVAPAMAEDGRPAVTLRLLHLNDIAEVGNGGGSGGLAEMKTLIEAEKARPGATAALVTFGGDLLSP
SLLSSIDKGAHMVEAMGLLGVDLAVPGNHEFDFGPKVFAERLSTSPFPWLASNLDGPALSTAEGAPITGLTSTAIRAVGP
YKIGFLGLLTPQTTVLSSPGPEVRFAEPLAAARAAVATLKAEGADVIIALTHQGQAEDRALAQALPEVAVVVGGHDHDSL
SWRESTGLVLKAGANARYLGVIDLRIDEQITSAGSAVVVLPEWSFRANVGLAPSPAVAARLAAWEAQLASDLDQPLGVTT
TELDSRRSVVRGAESTMGNLVTDAMRLATGAQAALTNGGGLRGDRLYNAGTALTRRDILAELPFGNVAVVLDLTGAQVKA
ALENGVSQIAQGAGRFPQVSGMEVLYDPALPVGERVVALQIDGRTVEPEGHYTVAVNDYIARGGDGYDALGGGRPIIDAA
AGRLLVTLVADHIAQAGRIGAQIEGRLVLRK

Specific function: Catalyzes the release of inorganic phosphate from 2',3'- cyclic nucleotides through consecutive 2',3'-phosphodiesterase and 3'- (or 2') nucleotidase activities. Also possesses a 5'- nucleotidase activity. Does not catalyze the release of inorganic phospha

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Secreted, cell wall; Peptidoglycan-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=540, Percent_Identity=27.962962962963, Blast_Score=160, Evalue=2e-39,
Organism=Escherichia coli, GI1786687, Length=524, Percent_Identity=27.0992366412214, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1790658, Length=504, Percent_Identity=21.031746031746, Blast_Score=62, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24652512, Length=489, Percent_Identity=29.4478527607362, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI19921980, Length=489, Percent_Identity=29.4478527607362, Blast_Score=210, Evalue=2e-54,
Organism=Drosophila melanogaster, GI161076508, Length=489, Percent_Identity=29.4478527607362, Blast_Score=209, Evalue=2e-54,
Organism=Drosophila melanogaster, GI19922446, Length=572, Percent_Identity=26.5734265734266, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI24654424, Length=572, Percent_Identity=26.5734265734266, Blast_Score=157, Evalue=1e-38,
Organism=Drosophila melanogaster, GI19922444, Length=504, Percent_Identity=26.7857142857143, Blast_Score=145, Evalue=7e-35,
Organism=Drosophila melanogaster, GI28573524, Length=511, Percent_Identity=26.4187866927593, Blast_Score=139, Evalue=5e-33,
Organism=Drosophila melanogaster, GI24641187, Length=448, Percent_Identity=25.8928571428571, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI221329836, Length=483, Percent_Identity=25.2587991718427, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR001899 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16; =3.1.3.5 [H]

Molecular weight: Translated: 52811; Mature: 52680

Theoretical pI: Translated: 4.90; Mature: 4.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAFSRWLVGFLLALSLVAPAMAEDGRPAVTLRLLHLNDIAEVGNGGGSGGLAEMKTLIEA
CCHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHH
EKARPGATAALVTFGGDLLSPSLLSSIDKGAHMVEAMGLLGVDLAVPGNHEFDFGPKVFA
HHCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHH
ERLSTSPFPWLASNLDGPALSTAEGAPITGLTSTAIRAVGPYKIGFLGLLTPQTTVLSSP
HHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCEEECCC
GPEVRFAEPLAAARAAVATLKAEGADVIIALTHQGQAEDRALAQALPEVAVVVGGHDHDS
CCCCEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCC
LSWRESTGLVLKAGANARYLGVIDLRIDEQITSAGSAVVVLPEWSFRANVGLAPSPAVAA
CCEECCCCEEEEECCCEEEEEEEEEEECHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHH
RLAAWEAQLASDLDQPLGVTTTELDSRRSVVRGAESTMGNLVTDAMRLATGAQAALTNGG
HHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCC
GLRGDRLYNAGTALTRRDILAELPFGNVAVVLDLTGAQVKAALENGVSQIAQGAGRFPQV
CCCCCCEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
SGMEVLYDPALPVGERVVALQIDGRTVEPEGHYTVAVNDYIARGGDGYDALGGGRPIIDA
CCCEEEECCCCCCCCEEEEEEECCEEECCCCCEEEEEECHHHCCCCCCCCCCCCCCHHHH
AAGRLLVTLVADHIAQAGRIGAQIEGRLVLRK
HHHHHHHHHHHHHHHHHCCCCCEEECEEEEEC
>Mature Secondary Structure 
AFSRWLVGFLLALSLVAPAMAEDGRPAVTLRLLHLNDIAEVGNGGGSGGLAEMKTLIEA
CHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEHHHHHHHCCCCCCCCHHHHHHHHHH
EKARPGATAALVTFGGDLLSPSLLSSIDKGAHMVEAMGLLGVDLAVPGNHEFDFGPKVFA
HHCCCCCCEEEEEECCHHCCHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCHHHHH
ERLSTSPFPWLASNLDGPALSTAEGAPITGLTSTAIRAVGPYKIGFLGLLTPQTTVLSSP
HHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHCCCEEEEEEEECCCCCEEECCC
GPEVRFAEPLAAARAAVATLKAEGADVIIALTHQGQAEDRALAQALPEVAVVVGGHDHDS
CCCCEECCHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCC
LSWRESTGLVLKAGANARYLGVIDLRIDEQITSAGSAVVVLPEWSFRANVGLAPSPAVAA
CCEECCCCEEEEECCCEEEEEEEEEEECHHHHCCCCEEEEEECCCCCCCCCCCCCHHHHH
RLAAWEAQLASDLDQPLGVTTTELDSRRSVVRGAESTMGNLVTDAMRLATGAQAALTNGG
HHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEECCCC
GLRGDRLYNAGTALTRRDILAELPFGNVAVVLDLTGAQVKAALENGVSQIAQGAGRFPQV
CCCCCCEECCCCHHHHHHHHHHCCCCCEEEEEEECCHHHHHHHHHHHHHHHHCCCCCCCC
SGMEVLYDPALPVGERVVALQIDGRTVEPEGHYTVAVNDYIARGGDGYDALGGGRPIIDA
CCCEEEECCCCCCCCEEEEEEECCEEECCCCCEEEEEECHHHCCCCCCCCCCCCCCHHHH
AAGRLLVTLVADHIAQAGRIGAQIEGRLVLRK
HHHHHHHHHHHHHHHHHCCCCCEEECEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969503; 9384377 [H]