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The map label for this gene is gloA [H]

Identifier: 83593801

GI number: 83593801

Start: 2866351

End: 2866791

Strand: Direct

Name: gloA [H]

Synonym: Rru_A2466

Alternate gene names: 83593801

Gene position: 2866351-2866791 (Clockwise)

Preceding gene: 83593800

Following gene: 83593803

Centisome position: 65.85

GC content: 63.49

Gene sequence:

>441_bases
ATGCGCTTCTTGCACACCATGGTCCGCGTCGCCGATCTCGACGCCTCCCTCGACTTCTATTGCGCCAAGCTCGGCCTGCG
CGAAATCAGCCGCCAGGATAACGAAAAGGGCCGGTTCACCCTGGTTTTCCTCGCCGCCCCCGCCGACGAGGAGACGGCCC
GCGAAACCAAGGCGCCCTTGGTCGAACTGACCTACAACTGGGATCCCGAGGTTTATACGGGCGGGCGCAACTTCGGGCAT
CTCGCCTATAAGGTCGATGACATCTATGCGACCTGCCAAAAGCTGATGGACGCCGGGGTGACGATCAACCGCCCGCCGCG
CGACGGCAATATGGCCTTCATCCGCTCGCCCGACGGCATTTCCATCGAACTGCTCCAAGAAGGCGCGCCGCTGGCGCCGG
CCGAGCCCTGGGCCAGCATGGCCAATACCGGAAGCTGGTAA

Upstream 100 bases:

>100_bases
CGCCTGTTTACCGACTCACTCCCGGGCGGGCGATGCCCTCGCCCGTCCGGGACCATGACCCGCGCGAGGCCCCCCTCGCG
CCCAGTCCGGGACTTTTCTC

Downstream 100 bases:

>100_bases
AACCTTTCCCTCCCCCCGGTTCGCCGGGGGGAAACGCCAAAAAGCCGCGTCCGGATCTTTCCTTGGGGGAAGATCCGGAC
GCGGCTTTTATAGCATAAGC

Product: glyoxalase/bleomycin resistance protein/dioxygenase

Products: NA

Alternate protein names: Aldoketomutase; Glyoxalase I; Glx I; Ketone-aldehyde mutase; Methylglyoxalase; S-D-lactoylglutathione methylglyoxal lyase [H]

Number of amino acids: Translated: 146; Mature: 146

Protein sequence:

>146_residues
MRFLHTMVRVADLDASLDFYCAKLGLREISRQDNEKGRFTLVFLAAPADEETARETKAPLVELTYNWDPEVYTGGRNFGH
LAYKVDDIYATCQKLMDAGVTINRPPRDGNMAFIRSPDGISIELLQEGAPLAPAEPWASMANTGSW

Sequences:

>Translated_146_residues
MRFLHTMVRVADLDASLDFYCAKLGLREISRQDNEKGRFTLVFLAAPADEETARETKAPLVELTYNWDPEVYTGGRNFGH
LAYKVDDIYATCQKLMDAGVTINRPPRDGNMAFIRSPDGISIELLQEGAPLAPAEPWASMANTGSW
>Mature_146_residues
MRFLHTMVRVADLDASLDFYCAKLGLREISRQDNEKGRFTLVFLAAPADEETARETKAPLVELTYNWDPEVYTGGRNFGH
LAYKVDDIYATCQKLMDAGVTINRPPRDGNMAFIRSPDGISIELLQEGAPLAPAEPWASMANTGSW

Specific function: Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione [H]

COG id: COG0346

COG function: function code E; Lactoylglutathione lyase and related lyases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glyoxalase I family [H]

Homologues:

Organism=Homo sapiens, GI118402586, Length=142, Percent_Identity=35.9154929577465, Blast_Score=81, Evalue=3e-16,
Organism=Escherichia coli, GI1787940, Length=132, Percent_Identity=37.1212121212121, Blast_Score=96, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6323639, Length=141, Percent_Identity=33.3333333333333, Blast_Score=81, Evalue=8e-17,
Organism=Drosophila melanogaster, GI20129743, Length=144, Percent_Identity=35.4166666666667, Blast_Score=83, Evalue=5e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004360
- InterPro:   IPR004361
- InterPro:   IPR018146 [H]

Pfam domain/function: PF00903 Glyoxalase [H]

EC number: =4.4.1.5 [H]

Molecular weight: Translated: 16284; Mature: 16284

Theoretical pI: Translated: 4.64; Mature: 4.64

Prosite motif: PS00934 GLYOXALASE_I_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLHTMVRVADLDASLDFYCAKLGLREISRQDNEKGRFTLVFLAAPADEETARETKAPL
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCE
VELTYNWDPEVYTGGRNFGHLAYKVDDIYATCQKLMDAGVTINRPPRDGNMAFIRSPDGI
EEEEECCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCC
SIELLQEGAPLAPAEPWASMANTGSW
EEEEECCCCCCCCCCCHHHHCCCCCC
>Mature Secondary Structure
MRFLHTMVRVADLDASLDFYCAKLGLREISRQDNEKGRFTLVFLAAPADEETARETKAPL
CHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCHHHHHHCCCE
VELTYNWDPEVYTGGRNFGHLAYKVDDIYATCQKLMDAGVTINRPPRDGNMAFIRSPDGI
EEEEECCCCCEEECCCCCCEEEEEHHHHHHHHHHHHHCCCEECCCCCCCCEEEEECCCCC
SIELLQEGAPLAPAEPWASMANTGSW
EEEEECCCCCCCCCCCHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]