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The map label for this gene is phbC [H]

Identifier: 83593748

GI number: 83593748

Start: 2814198

End: 2815994

Strand: Direct

Name: phbC [H]

Synonym: Rru_A2413

Alternate gene names: 83593748

Gene position: 2814198-2815994 (Clockwise)

Preceding gene: 83593742

Following gene: 83593749

Centisome position: 64.65

GC content: 61.6

Gene sequence:

>1797_bases
ATGGCCAATCAGGGCAGCGAGAAGACCCCTCCCGATCCGGCGCGGATCGGCAAGACCATGGCCGATATCGCAGAACGCAG
CCAGAAACTCGTCACCGATTTCATGACCCGCCAGGCGACCAGTCCGACCGGGGGAATGGATGATCCGATGAATATCGGGC
AGGCCTTCCTTGATATGACCCGGCAGATGATGTCCCACCCCGAACGCCTGGTCGAAGCCCAGATGAGCCTGTGGCAAGAC
TACCTCGCGTTGTGGCAGACCACGGCTTTGCGGATGATGGGCGCCGAGGCCCATCCGGTGGCCACCCCGGCCCAGGGCGA
TCGGCGGTTCAAGGACGAGGCCTGGGACAACAACGAGGTCTTCGATTTCATCAAGCAAACCTACCTGCTGACGGCGCGCT
GGATGCAGGGCGTCGTCCGTGATGTCGACGGCATGGATCGCCATACCGCCGAGAAGGTCGATTTCTATACGCGGCAGTTC
GTCGATGCCATGGCGCCGTCGAATTTCGCCCTAACCAACCCGGAAGTGATCCGCGAAACCCTTGATAGCGGCGGCGAAAA
TCTGGTGAAGGGCCTAGAGCACCTGCTCCACGACCTGGAGCGCGGCAAGGGCCAGTTGCGCATTTCGATGACCGATGAAA
GCGCCTTCGAGGTTGGGGTGAACGTGGCGGCGACCCCGGGCAAGGTCATCGCCCGCAACAGCCTGATGGAGCTTCTTCAA
TACACCCCGACCACCGAACAGGTCCACAAGCGGCCCCTGCTGATCATTCCGCCCTGGATCAACAAGTACTACATCCTGGA
TCTACGCCCCAAGAACAGCTTCATCAAATGGGCGATCGATCAGGGTCATAGCGTCTTCGTCATTTCGTGGGTCAACCCCG
AGGCCGATCTCGCCGCCAAGTCCTTCGAAGACTATATGGTGGAAGGCCCCTTGGCCGCGCTTGATATCATCAAGACGATC
ACCGGCGAGGACTCGGTCAACGCCATCGGCTATTGCCTGGGCGGCACACTGCTGGGATGCGTTCTGTCCTATCTGGAAGC
CAAGGGCCAGTCCGACCGCATCGCCTCGGCCACCCATTTCACCGCCATGCTCGATTTCAGCGAGCCCGGCGAACTGGGGA
TCTTCATCGACGACCAGAGCCTGACCTTCCTTGAGGGCAAGATGAACAAGGTCGGCTATCTCGATGGCAGCGAGATGGCC
ACCACCTTCAACATGCTGCGCGCCAATGATCTGATCTGGTCCTTCGTCGTCAATAACTACCTGATGGGCAAGGATCCCTT
CCCCTTCGATCTTTTGTACTGGAACGCCGACAGCACCCGCATGCCCGCGGCCATGCACAGCTTCTATCTGCGCAAGATGT
ACCAGGAAAACGTGCTGAAGGACCCCGGCGGCGTGACGCTGCTTGGCGTGCCCATCGACCTGCGGCGCAACCGCACGCCC
AGCTATTTCGTCAGCGCCCGCGAAGACCATATCGCCCCCTGGCGCTCGACCTTCGCCGGAGCCCAGCTTTACAGCGGCCC
GGTGCGCTTCGTTCTCGCCGCCTCGGGCCATATCGCCGGGGTGGTCAATCCGCCCTCGGCCAATAAATACTGCTTCTGGA
CCAATACCAAGAAGGCCAAGGATGCCGAATCCTGGCTGGAAAAGGCCAGCCAGACCGACGGCTCGTGGTGGACCGATTGG
GAGGCCTGGGTCGAGAAGTTCACCGAGGGCAAGGTTCCCGCCCGGGTTCCCGGCGACGCCGGCGTGCCGGTGCTGGGCGA
CGCCCCGGGCGATTACGTGAAGGTGCGCGCCGGCTAG

Upstream 100 bases:

>100_bases
CGACCAAGCTCACCCGTCTTGCGGGAACGTCAGGACAAACCACATAGCATTTCAGTCCAGGAACCCTTCTACCCTCTCCC
CTTCGCGCAAGGACATCAAC

Downstream 100 bases:

>100_bases
AATCGCTTCGGGTTTCCTCGCGCTTCGAGATGAGTCACACTCGGCGCCCGGATGGCTGACAAGGCCGTCCGGGCGTTTTT
CTTAAACGGGTGTTTCAAGG

Product: Poly(R)-hydroxyalkanoic acid synthase, class I

Products: NA

Alternate protein names: PHA synthase; PHB synthase; Poly(3-hydroxyalkanoate) polymerase; PHA polymerase; Poly(3-hydroxybutyrate) polymerase; PHB polymerase; Polyhydroxyalkanoic acid synthase [H]

Number of amino acids: Translated: 598; Mature: 597

Protein sequence:

>598_residues
MANQGSEKTPPDPARIGKTMADIAERSQKLVTDFMTRQATSPTGGMDDPMNIGQAFLDMTRQMMSHPERLVEAQMSLWQD
YLALWQTTALRMMGAEAHPVATPAQGDRRFKDEAWDNNEVFDFIKQTYLLTARWMQGVVRDVDGMDRHTAEKVDFYTRQF
VDAMAPSNFALTNPEVIRETLDSGGENLVKGLEHLLHDLERGKGQLRISMTDESAFEVGVNVAATPGKVIARNSLMELLQ
YTPTTEQVHKRPLLIIPPWINKYYILDLRPKNSFIKWAIDQGHSVFVISWVNPEADLAAKSFEDYMVEGPLAALDIIKTI
TGEDSVNAIGYCLGGTLLGCVLSYLEAKGQSDRIASATHFTAMLDFSEPGELGIFIDDQSLTFLEGKMNKVGYLDGSEMA
TTFNMLRANDLIWSFVVNNYLMGKDPFPFDLLYWNADSTRMPAAMHSFYLRKMYQENVLKDPGGVTLLGVPIDLRRNRTP
SYFVSAREDHIAPWRSTFAGAQLYSGPVRFVLAASGHIAGVVNPPSANKYCFWTNTKKAKDAESWLEKASQTDGSWWTDW
EAWVEKFTEGKVPARVPGDAGVPVLGDAPGDYVKVRAG

Sequences:

>Translated_598_residues
MANQGSEKTPPDPARIGKTMADIAERSQKLVTDFMTRQATSPTGGMDDPMNIGQAFLDMTRQMMSHPERLVEAQMSLWQD
YLALWQTTALRMMGAEAHPVATPAQGDRRFKDEAWDNNEVFDFIKQTYLLTARWMQGVVRDVDGMDRHTAEKVDFYTRQF
VDAMAPSNFALTNPEVIRETLDSGGENLVKGLEHLLHDLERGKGQLRISMTDESAFEVGVNVAATPGKVIARNSLMELLQ
YTPTTEQVHKRPLLIIPPWINKYYILDLRPKNSFIKWAIDQGHSVFVISWVNPEADLAAKSFEDYMVEGPLAALDIIKTI
TGEDSVNAIGYCLGGTLLGCVLSYLEAKGQSDRIASATHFTAMLDFSEPGELGIFIDDQSLTFLEGKMNKVGYLDGSEMA
TTFNMLRANDLIWSFVVNNYLMGKDPFPFDLLYWNADSTRMPAAMHSFYLRKMYQENVLKDPGGVTLLGVPIDLRRNRTP
SYFVSAREDHIAPWRSTFAGAQLYSGPVRFVLAASGHIAGVVNPPSANKYCFWTNTKKAKDAESWLEKASQTDGSWWTDW
EAWVEKFTEGKVPARVPGDAGVPVLGDAPGDYVKVRAG
>Mature_597_residues
ANQGSEKTPPDPARIGKTMADIAERSQKLVTDFMTRQATSPTGGMDDPMNIGQAFLDMTRQMMSHPERLVEAQMSLWQDY
LALWQTTALRMMGAEAHPVATPAQGDRRFKDEAWDNNEVFDFIKQTYLLTARWMQGVVRDVDGMDRHTAEKVDFYTRQFV
DAMAPSNFALTNPEVIRETLDSGGENLVKGLEHLLHDLERGKGQLRISMTDESAFEVGVNVAATPGKVIARNSLMELLQY
TPTTEQVHKRPLLIIPPWINKYYILDLRPKNSFIKWAIDQGHSVFVISWVNPEADLAAKSFEDYMVEGPLAALDIIKTIT
GEDSVNAIGYCLGGTLLGCVLSYLEAKGQSDRIASATHFTAMLDFSEPGELGIFIDDQSLTFLEGKMNKVGYLDGSEMAT
TFNMLRANDLIWSFVVNNYLMGKDPFPFDLLYWNADSTRMPAAMHSFYLRKMYQENVLKDPGGVTLLGVPIDLRRNRTPS
YFVSAREDHIAPWRSTFAGAQLYSGPVRFVLAASGHIAGVVNPPSANKYCFWTNTKKAKDAESWLEKASQTDGSWWTDWE
AWVEKFTEGKVPARVPGDAGVPVLGDAPGDYVKVRAG

Specific function: Polymerizes d(-)-3-hydroxybutyryl-CoA to create PHB which consists of thousands of hydroxybutyrate molecules linked end to end. PHB serves as an intracellular energy reserve material when cells grow under conditions of nutrient limitation [H]

COG id: COG3243

COG function: function code I; Poly(3-hydroxyalkanoate) synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PHA/PHB synthase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000073
- InterPro:   IPR010963
- InterPro:   IPR010941 [H]

Pfam domain/function: PF00561 Abhydrolase_1; PF07167 PhaC_N [H]

EC number: NA

Molecular weight: Translated: 66807; Mature: 66675

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANQGSEKTPPDPARIGKTMADIAERSQKLVTDFMTRQATSPTGGMDDPMNIGQAFLDMT
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
RQMMSHPERLVEAQMSLWQDYLALWQTTALRMMGAEAHPVATPAQGDRRFKDEAWDNNEV
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
FDFIKQTYLLTARWMQGVVRDVDGMDRHTAEKVDFYTRQFVDAMAPSNFALTNPEVIRET
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LDSGGENLVKGLEHLLHDLERGKGQLRISMTDESAFEVGVNVAATPGKVIARNSLMELLQ
HHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCEEECCCCHHHHHHHHHHHHH
YTPTTEQVHKRPLLIIPPWINKYYILDLRPKNSFIKWAIDQGHSVFVISWVNPEADLAAK
CCCCHHHHHCCCEEEECCCCCCEEEEEECCCCCEEEEEECCCCEEEEEEECCCCHHHHHH
SFEDYMVEGPLAALDIIKTITGEDSVNAIGYCLGGTLLGCVLSYLEAKGQSDRIASATHF
HHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEE
TAMLDFSEPGELGIFIDDQSLTFLEGKMNKVGYLDGSEMATTFNMLRANDLIWSFVVNNY
EEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
LMGKDPFPFDLLYWNADSTRMPAAMHSFYLRKMYQENVLKDPGGVTLLGVPIDLRRNRTP
ECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCC
SYFVSAREDHIAPWRSTFAGAQLYSGPVRFVLAASGHIAGVVNPPSANKYCFWTNTKKAK
CEEEECCCCCCCCHHHHHCCCHHHCCCEEEEEEECCCEEEEECCCCCCEEEEECCCCCCC
DAESWLEKASQTDGSWWTDWEAWVEKFTEGKVPARVPGDAGVPVLGDAPGDYVKVRAG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEEEECC
>Mature Secondary Structure 
ANQGSEKTPPDPARIGKTMADIAERSQKLVTDFMTRQATSPTGGMDDPMNIGQAFLDMT
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHH
RQMMSHPERLVEAQMSLWQDYLALWQTTALRMMGAEAHPVATPAQGDRRFKDEAWDNNEV
HHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCHH
FDFIKQTYLLTARWMQGVVRDVDGMDRHTAEKVDFYTRQFVDAMAPSNFALTNPEVIRET
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LDSGGENLVKGLEHLLHDLERGKGQLRISMTDESAFEVGVNVAATPGKVIARNSLMELLQ
HHCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHCCEEECCCCHHHHHHHHHHHHH
YTPTTEQVHKRPLLIIPPWINKYYILDLRPKNSFIKWAIDQGHSVFVISWVNPEADLAAK
CCCCHHHHHCCCEEEECCCCCCEEEEEECCCCCEEEEEECCCCEEEEEEECCCCHHHHHH
SFEDYMVEGPLAALDIIKTITGEDSVNAIGYCLGGTLLGCVLSYLEAKGQSDRIASATHF
HHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHEE
TAMLDFSEPGELGIFIDDQSLTFLEGKMNKVGYLDGSEMATTFNMLRANDLIWSFVVNNY
EEEEECCCCCCEEEEECCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCE
LMGKDPFPFDLLYWNADSTRMPAAMHSFYLRKMYQENVLKDPGGVTLLGVPIDLRRNRTP
ECCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCCHHCCCCCC
SYFVSAREDHIAPWRSTFAGAQLYSGPVRFVLAASGHIAGVVNPPSANKYCFWTNTKKAK
CEEEECCCCCCCCHHHHHCCCHHHCCCEEEEEEECCCEEEEECCCCCCEEEEECCCCCCC
DAESWLEKASQTDGSWWTDWEAWVEKFTEGKVPARVPGDAGVPVLGDAPGDYVKVRAG
HHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9748438 [H]