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The map label for this gene is papS

Identifier: 82751056

GI number: 82751056

Start: 1449798

End: 1451000

Strand: Reverse

Name: papS

Synonym: SAB1321c

Alternate gene names: 82751056

Gene position: 1451000-1449798 (Counterclockwise)

Preceding gene: 82751057

Following gene: 82751055

Centisome position: 52.91

GC content: 31.75

Gene sequence:

>1203_bases
ATGGATAAATCATTATTTGAACAGGCAAGGCCTATATTAGAACAAATTCAAGACAATGGTTTTGAAGCATATTATGTAGG
TGGCTCTGTAAGAGATTATGTCATGGGAAGAAATATTCATGATATAGATATCACAACAAGTGCAACGCCAGATGAAATAG
AATCTATCTTTAGTCATACGATACCTGTAGGTAAAGAACATGGCACCATAAATGTAGTTTTTAATAATGAAAATTATGAA
GTGACAACATTCCGGGCTGAAGAAGATTATGTCGATCACCGTAGACCAAGTGGTGTTACATTTGTTCGTGATTTATACGA
AGATTTGCAACGAAGAGATTTCACGATGAATGCGATAGCAATGGATACAGCATACAAATTGTATGATTATTTTGATGGTC
AACAAGATATTAATAATCGAATAATAAGAACTGTAGGTATAGCTGCGGAACGATTCCAAGAAGATGCTTTACGCATGATT
CGATGTTTAAGATTCCAGTCACAATTATCATTTGAGATTGCAACGGAAACATTTGAAGCGATGCGTACACAAATGGCAGA
TATTAAATTTTTATCAATTGAGCGTATAGTGATTGAACTAACTAAATTAATGCGAGGTATTAATGTTGAAAAGAGTTTTA
ATCATTTAAAATCGCTGAAAGCATTTAATTATATGCCATATTTCGAACATCTTGATATGAATCAAATTAATGTGACTGAA
CCAATTGATTTAGAATTGTTGATTGCTATAGTGTCAGTTAAATTTGATATTAATTACTCATTAAAGCCTTTAAAGCTAAG
TAACCGACAAGTAAAAGATATCAATCAATATATTCAAATTATGAATGCATTACCAAGTATTTTTACAAAAGAACAATTAA
AAATCTTTGTTTATGATTATGATACGAATCTCATTAAAAATATAATGGTTGCAGCAGACGTGTTAAAGGCAAATGATATT
CAAGGACATGAACCATTAATCGTTAACCTTCAAACGATTGATGAAACATTACATCACTTACCTATGCATAATAGAAAAGA
CATGATGGTTAATGGCGGTGTACTAATGGCACATTTAAATGCCAAAAGTGGTCCGTGGTTAAAAGATGTGCTAAGACAAA
TTGAGATAGCGATTGTAACAGGTAAAGTAAGCAACGAAGAAACTGAAATTTTGAAATGGGTGGATAATCATGTCAAAATA
TAG

Upstream 100 bases:

>100_bases
ATTGCTGAACGTTTTGGATCAGAACTCATTACAGATCAATATGAGTATTATTATCAAAAAATGCTAAATGAGCATAACAA
AAGTAAAGGCGAGTAATAAT

Downstream 100 bases:

>100_bases
TCAAGATGTACTTCAATTACTCTATAAAAATAAACCGAATTATATATCTGGACAAAGCATTGCGGAATCACTTAATATAT
CGCGCACTGCAGTAAAAAAA

Product: tRNA CCA-pyrophosphorylase

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 400; Mature: 400

Protein sequence:

>400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNNENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAAERFQEDALRMI
RCLRFQSQLSFEIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIFTKEQLKIFVYDYDTNLIKNIMVAADVLKANDI
QGHEPLIVNLQTIDETLHHLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Sequences:

>Translated_400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNNENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAAERFQEDALRMI
RCLRFQSQLSFEIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIFTKEQLKIFVYDYDTNLIKNIMVAADVLKANDI
QGHEPLIVNLQTIDETLHHLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
>Mature_400_residues
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHTIPVGKEHGTINVVFNNENYE
VTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIAMDTAYKLYDYFDGQQDINNRIIRTVGIAAERFQEDALRMI
RCLRFQSQLSFEIATETFEAMRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIFTKEQLKIFVYDYDTNLIKNIMVAADVLKANDI
QGHEPLIVNLQTIDETLHHLPMHNRKDMMVNGGVLMAHLNAKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=276, Percent_Identity=33.3333333333333, Blast_Score=122, Evalue=4e-28,
Organism=Escherichia coli, GI87081691, Length=190, Percent_Identity=34.2105263157895, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1789436, Length=218, Percent_Identity=30.2752293577982, Blast_Score=82, Evalue=9e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=208, Percent_Identity=34.6153846153846, Blast_Score=103, Evalue=1e-22,
Organism=Saccharomyces cerevisiae, GI6321016, Length=206, Percent_Identity=27.6699029126214, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI281360112, Length=226, Percent_Identity=33.1858407079646, Blast_Score=104, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21357337, Length=226, Percent_Identity=33.1858407079646, Blast_Score=104, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 46394; Mature: 46394

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
IPVGKEHGTINVVFNNENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIA
CCCCCCCCEEEEEEECCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHHEE
MDTAYKLYDYFDGQQDINNRIIRTVGIAAERFQEDALRMIRCLRFQSQLSFEIATETFEA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIFTKEQLKIFVYDY
CCCHHHHEEEHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DTNLIKNIMVAADVLKANDIQGHEPLIVNLQTIDETLHHLPMHNRKDMMVNGGVLMAHLN
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEECCEEEEEEC
AKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
CCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MDKSLFEQARPILEQIQDNGFEAYYVGGSVRDYVMGRNIHDIDITTSATPDEIESIFSHT
CCHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHHHCCCCEEEEEECCCCHHHHHHHHHHC
IPVGKEHGTINVVFNNENYEVTTFRAEEDYVDHRRPSGVTFVRDLYEDLQRRDFTMNAIA
CCCCCCCCEEEEEEECCCEEEEEEECCHHHHHCCCCCCHHHHHHHHHHHHHCCCEEHHEE
MDTAYKLYDYFDGQQDINNRIIRTVGIAAERFQEDALRMIRCLRFQSQLSFEIATETFEA
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MRTQMADIKFLSIERIVIELTKLMRGINVEKSFNHLKSLKAFNYMPYFEHLDMNQINVTE
HHHHHHCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHCCCCCCCCCC
PIDLELLIAIVSVKFDINYSLKPLKLSNRQVKDINQYIQIMNALPSIFTKEQLKIFVYDY
CCCHHHHEEEHHEEEECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
DTNLIKNIMVAADVLKANDIQGHEPLIVNLQTIDETLHHLPMHNRKDMMVNGGVLMAHLN
CHHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHHHHHHHHCCCCCCCCEEEECCEEEEEEC
AKSGPWLKDVLRQIEIAIVTGKVSNEETEILKWVDNHVKI
CCCCCHHHHHHHHHEEEEEECCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA