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The map label for this gene is aes [H]

Identifier: 82750357

GI number: 82750357

Start: 670003

End: 671046

Strand: Direct

Name: aes [H]

Synonym: SAB0604

Alternate gene names: 82750357

Gene position: 670003-671046 (Clockwise)

Preceding gene: 82750356

Following gene: 82750358

Centisome position: 24.43

GC content: 30.56

Gene sequence:

>1044_bases
ATGAATAAAGATAATAAATGGACGATGATAACTGCGCTTTTTATAACTGTAATCAGTGTATTGTTAGCATTTCATCTGAA
ACAACATTATGACCAAACTACAAATGAGAACCATGCTAATAAAGACAAAATTAATATTAAAAATAAAAATGTGCGCATTT
ATCAAAACCTTACATACAATAGAGTTTTCCCTAACAGTAAATTAGATATTATTACACCTGTTGATATGTCTTCTAATGCC
AAACTGCCAGTTATTTTTTGGATGCACGGTGGTGGTTATATTGCGGGTGATAAGCAGTATAAAAACCCATTATTAGCGAA
AATTGCTGAACAAGGGTACATTGTTGTGAATGTAAATTATGCATTGGCGCCACAATATAAATATCCCACTCCATTAATTC
AAATGAATCAAGCAACTCAATTCATTAAAGAAAATAAAATGAATTTACCTATTGATTTTAATCAAGTAATTATTGGCGGT
GATTCTGCAGGTGCTCAATTAGCTAGCCAATTTACTGCAATACAGACAAACAATCAATTAAGAGAAGCCATGAAATTTGA
TCAGTCATTCAAACCATCGCAAATTAAAGGTGCTATACTATTTGGTGGTTTTTATAATATGCAAACAGTTAGAGAAACTG
AGTTTCCAAGAATACAGTTATTTATGAAAAGTTATACTGGCGAAGAAGATTGGGAAAAGAGTTTTAAAAATATTTCACAA
ATGTCGACAGTAAAACAATCGACAAAAAATTATCCACCAACATTTTTATCTGTTGGAGATAGCGATCCATTCGAAAGTCA
AAATATAGAATTCAGTAAGAAATTACAAGAATTGAATGTACCAGTAGATACTTTGTTTTATGATGGTACGCATCATTTAC
ATCATCAGTATCAATTTCACCTTAATAAACCTGAATCGATAGATAATATCAAAAAAGTGTTACTTTTCTTAAGTCGTAAT
ACATCTTCTAGTGGTATTCAAACTGAAGAGAAACCACAAATAGAAAATCCGAGTAATGAATTACCGTTAAATCCTTTAAA
CTAA

Upstream 100 bases:

>100_bases
CCTTAAAAGCAATTCGTCTCTACTCTTATCGGGCGAATTGCTTTTTATATTTATTCAGTCTATTAATATGAGCGTCTAAC
AAATAGAGAGGTACGATGTA

Downstream 100 bases:

>100_bases
TGATAAACAGTAGTAATTTATTACTTAAGAAACATTTAAGATCTTCAAATTGAAAACGAAGAATTTAAAACATGTGGGGC
TAATGTGTAAGTGTCTGAGA

Product: lipase

Products: alcohol; a carboxylate [C]

Alternate protein names: NA

Number of amino acids: Translated: 347; Mature: 347

Protein sequence:

>347_residues
MNKDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKINIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNA
KLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
DSAGAQLASQFTAIQTNNQLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQ
MSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRN
TSSSGIQTEEKPQIENPSNELPLNPLN

Sequences:

>Translated_347_residues
MNKDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKINIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNA
KLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
DSAGAQLASQFTAIQTNNQLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQ
MSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRN
TSSSGIQTEEKPQIENPSNELPLNPLN
>Mature_347_residues
MNKDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKINIKNKNVRIYQNLTYNRVFPNSKLDIITPVDMSSNA
KLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNYALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGG
DSAGAQLASQFTAIQTNNQLREAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQ
MSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFHLNKPESIDNIKKVLLFLSRN
TSSSGIQTEEKPQIENPSNELPLNPLN

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.- [C]

Molecular weight: Translated: 39798; Mature: 39798

Theoretical pI: Translated: 9.19; Mature: 9.19

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNKDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKINIKNKNVRIYQNLTYN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEECCEEE
RVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNY
EECCCCCEEEEECCCCCCCCCCEEEEEECCCEEEECCHHHCCHHHHHHHHCCEEEEEEEE
ALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGGDSAGAQLASQFTAIQTNNQL
EECCCCCCCCCCEEHHHHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHCCHHH
REAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQ
HHHHHHCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHH
MSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFH
HHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCHHHEEECCCHHCCEEEEEE
LNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQIENPSNELPLNPLN
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNKDNKWTMITALFITVISVLLAFHLKQHYDQTTNENHANKDKINIKNKNVRIYQNLTYN
CCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCEEEEECCEEE
RVFPNSKLDIITPVDMSSNAKLPVIFWMHGGGYIAGDKQYKNPLLAKIAEQGYIVVNVNY
EECCCCCEEEEECCCCCCCCCCEEEEEECCCEEEECCHHHCCHHHHHHHHCCEEEEEEEE
ALAPQYKYPTPLIQMNQATQFIKENKMNLPIDFNQVIIGGDSAGAQLASQFTAIQTNNQL
EECCCCCCCCCCEEHHHHHHHHHHCCCCCCEECCEEEEECCCHHHHHHHHHHHHHCCHHH
REAMKFDQSFKPSQIKGAILFGGFYNMQTVRETEFPRIQLFMKSYTGEEDWEKSFKNISQ
HHHHHHCCCCCCCCCCEEEEECCCCCCCHHHCCCCCCEEHHHHHCCCCHHHHHHHHHHHH
MSTVKQSTKNYPPTFLSVGDSDPFESQNIEFSKKLQELNVPVDTLFYDGTHHLHHQYQFH
HHHHHHHHCCCCCCEEECCCCCCCCCCCCHHHHHHHHHCCCHHHEEECCCHHCCEEEEEE
LNKPESIDNIKKVLLFLSRNTSSSGIQTEEKPQIENPSNELPLNPLN
CCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Carboxylic ester; H2O [C]

Specific reaction: Carboxylic ester + H2O = an alcohol + carboxylate [C]

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA