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The map label for this gene is cysG [H]

Identifier: 82703430

GI number: 82703430

Start: 2631218

End: 2632726

Strand: Reverse

Name: cysG [H]

Synonym: Nmul_A2313

Alternate gene names: 82703430

Gene position: 2632726-2631218 (Counterclockwise)

Preceding gene: 82703437

Following gene: 82703427

Centisome position: 82.68

GC content: 57.59

Gene sequence:

>1509_bases
ATGCTGCAGCCAGGTTCACGGCGCCCTAACCCGGAAGAGTATTTTCGGGTAAAGTATGGAAAAGACTTCTTCCTCATTCA
AGCCAAAAAAGTGAATTTCCTGCCGATTTTTCTCGATATCCATGATCGCAACTGTGTGGTGGCAGGCGGAGGAGAAGTCG
CCGCGCGCAAGGTCGCAATGCTGCTACGCGCAGGAGCGCACGTTACCGTAGTCGCTCCGCGGCTTTGTTCCGAACTGCTG
GAACAACTGAATGAACAATCACGGGAAGGAAAGCTGGCTTACCGTGCCGAAACTTTCCAGGACGAGCATCTGGCAGACGC
TGTTCTCGTCATCGCGGCCACGGATGATTTCGCCGTCAATCAAAGAATCTCGGAAGCAGCCAAGCGGCTGCGCATTCCTG
TCAATGTCGTCGATAATCCTGGCCTATGCTCCTTCATCATGCCGTCGATCGTGGATCGGACGCCGGTTGTTGTCGCGGTT
TCGAGCGGGGGTGCATCCCCTGTTCTGACAAGGCTGCTGCGAGCGCGTCTGGAAACCATGATCCCTATGGCTTACGGACG
CCTGGCTGCATACGCTGGCGAGTTCCGCGACAGGGTAAAACATCGTTTTTCGCAGCCGGCCCAGCGCCGCCGGTTCTGGG
AGCGGGTATTGCAGGGACCGTTTGCGGAAATGGTGTTCGCAGGCAAGGATCAGGCAGCAAAGGCCTGGCTCGAGCGTGAG
CTCGAAAATGAAACCGAAGAACCGGTCAAGGGAGAGGTGTATCTGGTAGGGGCCGGGCCCGGCGACCCTGAACTGCTGAC
TTTTAGGGCCATGCGACTCATGCAGCAGGCAGACGTGGTCGTGTACGATAGGCTCGTGTCACCGGAAATCCTGGATATGC
TGCGGCGCGATACTCCCCGGATTTATGCCGGCAAGGAACGTAACCGCCACACGATGCCGCAGGAGTCGATCAATGATTTG
CTGGTGCGCCTGGCTAAGGAAGGCAAGCGCGTGTTGCGCCTGAAAGGTGGAGACCCGTTTATTTTTGGTCGCGGTGGCGA
GGAAATCGAAACGCTTGCGGGACACGGAATTCCCTTCGAAGTGGTGCCCGGCATTACTGCTGCTAATGGGGCTGCATCCT
ATGCAGGCATTCCGCTCACACATCGGGATTACGCCCAGTCCTGTATATTCGTTACCGGACACCTGAAGGATGGCAGTGTG
GATCTGGACTGGCCAATGCTGGCGCGACCGAAGCAGACCATCGTGGTTTACATGGGATTGCTTGGATTGACCGTGCTATG
CAAACAACTGATCGCACACGGTCTGCCCAATAACACTCCCGCTGCAATCGTGCAGCAGGGCACCACCCGCAAGCAGCGGG
TGCTTGCCGCTACCCTCGAAACGCTTCCCGAGTTGACCGCTGCCGCCCATCTCGTTCCCCCCACTCTCGTTATCGTGGGG
GAGGTTGTGAATCTGCACCGAAAACTCGCCTGGTTCCAGCCGGAGGGGAATCTACCCGGCGCAGAGTGA

Upstream 100 bases:

>100_bases
CGCACGAAGCGAGAGAATGCATCATAGCAAATAAGGCGCGGTATGAAAAATTTACCTGGAAAAGAAGCCAAATTCATAAC
CTCGACTGATACAACCGGCC

Downstream 100 bases:

>100_bases
AGTTATTACGGGCGGGTATTCTGCAAGAAGCATTCAATATCAGACTGGACTTTACCCAGGGTCGACCCCGGGCAGAGGCG
GACTCCTGAGCTGCATACCG

Product: uroporphyrin-III C-methyltransferase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase [H]

Number of amino acids: Translated: 502; Mature: 502

Protein sequence:

>502_residues
MLQPGSRRPNPEEYFRVKYGKDFFLIQAKKVNFLPIFLDIHDRNCVVAGGGEVAARKVAMLLRAGAHVTVVAPRLCSELL
EQLNEQSREGKLAYRAETFQDEHLADAVLVIAATDDFAVNQRISEAAKRLRIPVNVVDNPGLCSFIMPSIVDRTPVVVAV
SSGGASPVLTRLLRARLETMIPMAYGRLAAYAGEFRDRVKHRFSQPAQRRRFWERVLQGPFAEMVFAGKDQAAKAWLERE
LENETEEPVKGEVYLVGAGPGDPELLTFRAMRLMQQADVVVYDRLVSPEILDMLRRDTPRIYAGKERNRHTMPQESINDL
LVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAGHGIPFEVVPGITAANGAASYAGIPLTHRDYAQSCIFVTGHLKDGSV
DLDWPMLARPKQTIVVYMGLLGLTVLCKQLIAHGLPNNTPAAIVQQGTTRKQRVLAATLETLPELTAAAHLVPPTLVIVG
EVVNLHRKLAWFQPEGNLPGAE

Sequences:

>Translated_502_residues
MLQPGSRRPNPEEYFRVKYGKDFFLIQAKKVNFLPIFLDIHDRNCVVAGGGEVAARKVAMLLRAGAHVTVVAPRLCSELL
EQLNEQSREGKLAYRAETFQDEHLADAVLVIAATDDFAVNQRISEAAKRLRIPVNVVDNPGLCSFIMPSIVDRTPVVVAV
SSGGASPVLTRLLRARLETMIPMAYGRLAAYAGEFRDRVKHRFSQPAQRRRFWERVLQGPFAEMVFAGKDQAAKAWLERE
LENETEEPVKGEVYLVGAGPGDPELLTFRAMRLMQQADVVVYDRLVSPEILDMLRRDTPRIYAGKERNRHTMPQESINDL
LVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAGHGIPFEVVPGITAANGAASYAGIPLTHRDYAQSCIFVTGHLKDGSV
DLDWPMLARPKQTIVVYMGLLGLTVLCKQLIAHGLPNNTPAAIVQQGTTRKQRVLAATLETLPELTAAAHLVPPTLVIVG
EVVNLHRKLAWFQPEGNLPGAE
>Mature_502_residues
MLQPGSRRPNPEEYFRVKYGKDFFLIQAKKVNFLPIFLDIHDRNCVVAGGGEVAARKVAMLLRAGAHVTVVAPRLCSELL
EQLNEQSREGKLAYRAETFQDEHLADAVLVIAATDDFAVNQRISEAAKRLRIPVNVVDNPGLCSFIMPSIVDRTPVVVAV
SSGGASPVLTRLLRARLETMIPMAYGRLAAYAGEFRDRVKHRFSQPAQRRRFWERVLQGPFAEMVFAGKDQAAKAWLERE
LENETEEPVKGEVYLVGAGPGDPELLTFRAMRLMQQADVVVYDRLVSPEILDMLRRDTPRIYAGKERNRHTMPQESINDL
LVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAGHGIPFEVVPGITAANGAASYAGIPLTHRDYAQSCIFVTGHLKDGSV
DLDWPMLARPKQTIVVYMGLLGLTVLCKQLIAHGLPNNTPAAIVQQGTTRKQRVLAATLETLPELTAAAHLVPPTLVIVG
EVVNLHRKLAWFQPEGNLPGAE

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789768, Length=462, Percent_Identity=52.3809523809524, Blast_Score=464, Evalue=1e-132,
Organism=Saccharomyces cerevisiae, GI6322922, Length=287, Percent_Identity=33.1010452961672, Blast_Score=135, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043 [H]

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase [H]

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4 [H]

Molecular weight: Translated: 55506; Mature: 55506

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQPGSRRPNPEEYFRVKYGKDFFLIQAKKVNFLPIFLDIHDRNCVVAGGGEVAARKVAM
CCCCCCCCCCHHHHEEEEECCEEEEEEECCCCEEEEEEEEECCCEEEECCCHHHHHHHHH
LLRAGAHVTVVAPRLCSELLEQLNEQSREGKLAYRAETFQDEHLADAVLVIAATDDFAVN
HHHCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHCCEEEEEEECCCHHHH
QRISEAAKRLRIPVNVVDNPGLCSFIMPSIVDRTPVVVAVSSGGASPVLTRLLRARLETM
HHHHHHHHHEECCEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
IPMAYGRLAAYAGEFRDRVKHRFSQPAQRRRFWERVLQGPFAEMVFAGKDQAAKAWLERE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHH
LENETEEPVKGEVYLVGAGPGDPELLTFRAMRLMQQADVVVYDRLVSPEILDMLRRDTPR
HHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCC
IYAGKERNRHTMPQESINDLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAGHGIPFE
EEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHCCCCCEE
VVPGITAANGAASYAGIPLTHRDYAQSCIFVTGHLKDGSVDLDWPMLARPKQTIVVYMGL
ECCCCEECCCCCCCCCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
LGLTVLCKQLIAHGLPNNTPAAIVQQGTTRKQRVLAATLETLPELTAAAHLVPPTLVIVG
HHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EVVNLHRKLAWFQPEGNLPGAE
HHHHHHHHHEEECCCCCCCCCC
>Mature Secondary Structure
MLQPGSRRPNPEEYFRVKYGKDFFLIQAKKVNFLPIFLDIHDRNCVVAGGGEVAARKVAM
CCCCCCCCCCHHHHEEEEECCEEEEEEECCCCEEEEEEEEECCCEEEECCCHHHHHHHHH
LLRAGAHVTVVAPRLCSELLEQLNEQSREGKLAYRAETFQDEHLADAVLVIAATDDFAVN
HHHCCCCEEEECHHHHHHHHHHHHHHHHCCCEEEEEHHCCHHHHCCEEEEEEECCCHHHH
QRISEAAKRLRIPVNVVDNPGLCSFIMPSIVDRTPVVVAVSSGGASPVLTRLLRARLETM
HHHHHHHHHEECCEEECCCCCHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHH
IPMAYGRLAAYAGEFRDRVKHRFSQPAQRRRFWERVLQGPFAEMVFAGKDQAAKAWLERE
HHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHH
LENETEEPVKGEVYLVGAGPGDPELLTFRAMRLMQQADVVVYDRLVSPEILDMLRRDTPR
HHCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCC
IYAGKERNRHTMPQESINDLLVRLAKEGKRVLRLKGGDPFIFGRGGEEIETLAGHGIPFE
EEECCCCCCCCCCHHHHHHHHHHHHHCCCEEEEEECCCEEEECCCCCHHHHHHCCCCCEE
VVPGITAANGAASYAGIPLTHRDYAQSCIFVTGHLKDGSVDLDWPMLARPKQTIVVYMGL
ECCCCEECCCCCCCCCCCCCCHHHHCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHH
LGLTVLCKQLIAHGLPNNTPAAIVQQGTTRKQRVLAATLETLPELTAAAHLVPPTLVIVG
HHHHHHHHHHHHCCCCCCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
EVVNLHRKLAWFQPEGNLPGAE
HHHHHHHHHEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA