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The map label for this gene is pcm [H]

Identifier: 82703402

GI number: 82703402

Start: 2598765

End: 2599442

Strand: Reverse

Name: pcm [H]

Synonym: Nmul_A2284

Alternate gene names: 82703402

Gene position: 2599442-2598765 (Counterclockwise)

Preceding gene: 82703405

Following gene: 82703401

Centisome position: 81.63

GC content: 55.01

Gene sequence:

>678_bases
TTGGATATGGATCTCGAACAAAGCCGGTATAACATGGTGGAGCAGCAAATTCGCACGTGGGAAGTGCTGGATCAGGAGGT
TCTCCAACTATTGTTCGAGCTGCGGCGCGAGGAATTTGTCCCGGCAGCTTACCGTTCACTTGCATTCGTCGATATGGAAA
TTCCGCTGGGATACGGCGAGGTAATGCTTGCCCCCAAGGTTGAGGCGCGGATTCTGCAGGAGTTGAGAATCAATAATACT
GACAGAATTCTGGAGGTAGGGAGCGGCAGTGGCTACCTGACTGCGCTGCTTGCTAAAAAGGGGAAGTTTGTCCATAGCGT
GGAGATTGTGCCGGAACTGGCAGCCATGGCCGAAAAAAACCTGCGGAACCACCAGATTGCCAACGTTCTGATAGAAAATG
GCGATGCTGCCCGCGGCTGGGGCCAGCACGGTCCCTATGACGTTATCGTCCTGACCGGATCCACTCCGGTGCTGCCGGAG
GGATTTCAGAAAAGCCTTAAAACCGGCGGGCGTCTCTTTGCTGTAGTGGGCGATCCACCGGTAATGCAGGCTCTTCTGGT
TACTTGCGTGGCCCAGGAGGAAAACGGGCGCGGGGGCGCGTATAGCACGGTCGGCCTGTTCGAAACCTGTATTGCGCCAC
TCAGAAATGCGAAGCAGCCGGCAAGATTTACATTCTGA

Upstream 100 bases:

>100_bases
CGAGTGCTCGCGGAAACGGACCCCTAGCTGCTGCTGTGAAGCTAACGGAAATGTGGAATAATTGCAAGTTTGTTATGACA
TTTCATATAGGAGCAGATGG

Downstream 100 bases:

>100_bases
TCTTTCGGCCCGCACTTCAGGATTGCCGTCCACGCGACGTATCCAGGGGTACTCTCCTATAGCTATCCGTCTATCTTTAA
CTGTAGTCAACGAAGATGTT

Product: protein-L-isoaspartate(D-aspartate) O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 225; Mature: 225

Protein sequence:

>225_residues
MDMDLEQSRYNMVEQQIRTWEVLDQEVLQLLFELRREEFVPAAYRSLAFVDMEIPLGYGEVMLAPKVEARILQELRINNT
DRILEVGSGSGYLTALLAKKGKFVHSVEIVPELAAMAEKNLRNHQIANVLIENGDAARGWGQHGPYDVIVLTGSTPVLPE
GFQKSLKTGGRLFAVVGDPPVMQALLVTCVAQEENGRGGAYSTVGLFETCIAPLRNAKQPARFTF

Sequences:

>Translated_225_residues
MDMDLEQSRYNMVEQQIRTWEVLDQEVLQLLFELRREEFVPAAYRSLAFVDMEIPLGYGEVMLAPKVEARILQELRINNT
DRILEVGSGSGYLTALLAKKGKFVHSVEIVPELAAMAEKNLRNHQIANVLIENGDAARGWGQHGPYDVIVLTGSTPVLPE
GFQKSLKTGGRLFAVVGDPPVMQALLVTCVAQEENGRGGAYSTVGLFETCIAPLRNAKQPARFTF
>Mature_225_residues
MDMDLEQSRYNMVEQQIRTWEVLDQEVLQLLFELRREEFVPAAYRSLAFVDMEIPLGYGEVMLAPKVEARILQELRINNT
DRILEVGSGSGYLTALLAKKGKFVHSVEIVPELAAMAEKNLRNHQIANVLIENGDAARGWGQHGPYDVIVLTGSTPVLPE
GFQKSLKTGGRLFAVVGDPPVMQALLVTCVAQEENGRGGAYSTVGLFETCIAPLRNAKQPARFTF

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1789100, Length=170, Percent_Identity=34.1176470588235, Blast_Score=108, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI71983477, Length=159, Percent_Identity=29.559748427673, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193207222, Length=100, Percent_Identity=34, Blast_Score=65, Evalue=4e-11,
Organism=Drosophila melanogaster, GI17981723, Length=179, Percent_Identity=30.7262569832402, Blast_Score=67, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 24947; Mature: 24947

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDMDLEQSRYNMVEQQIRTWEVLDQEVLQLLFELRREEFVPAAYRSLAFVDMEIPLGYGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEEECCCCCCC
VMLAPKVEARILQELRINNTDRILEVGSGSGYLTALLAKKGKFVHSVEIVPELAAMAEKN
EEECCHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
LRNHQIANVLIENGDAARGWGQHGPYDVIVLTGSTPVLPEGFQKSLKTGGRLFAVVGDPP
CCHHHHHHHEEECCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCH
VMQALLVTCVAQEENGRGGAYSTVGLFETCIAPLRNAKQPARFTF
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MDMDLEQSRYNMVEQQIRTWEVLDQEVLQLLFELRREEFVPAAYRSLAFVDMEIPLGYGE
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEEEEEECCCCCCC
VMLAPKVEARILQELRINNTDRILEVGSGSGYLTALLAKKGKFVHSVEIVPELAAMAEKN
EEECCHHHHHHHHHHHCCCCHHEEEECCCCCHHHHHHHHCCCCEEHHHHHHHHHHHHHHH
LRNHQIANVLIENGDAARGWGQHGPYDVIVLTGSTPVLPEGFQKSLKTGGRLFAVVGDPP
CCHHHHHHHEEECCCCCCCCCCCCCEEEEEECCCCCCCCHHHHHHHCCCCEEEEEECCCH
VMQALLVTCVAQEENGRGGAYSTVGLFETCIAPLRNAKQPARFTF
HHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14621292 [H]