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The map label for this gene is ubiE

Identifier: 82703387

GI number: 82703387

Start: 2581010

End: 2581744

Strand: Reverse

Name: ubiE

Synonym: Nmul_A2269

Alternate gene names: 82703387

Gene position: 2581744-2581010 (Counterclockwise)

Preceding gene: 82703388

Following gene: 82703386

Centisome position: 81.08

GC content: 54.42

Gene sequence:

>735_bases
ATGACGAGAACTACCCATTTCGGCTTTAAGACCGTGTCGGAAACGGAGAAAGCACGCGAGGTAGCAGGTGTGTTTGATTC
TGTCGCGGAACGTTACAACCTGATGAATGACCTCATGTCGGGGGGCATGCATCGTCTGTGGAAACGATTTGCGATTGAGG
TGAGCGGAGTTCGGACAGGGGAGCGTGTACTGGATATTGCCGGGGGAACGGCGGATCTGAGCTCGCTCTTCCTTGAGCAG
GTGGGGAGCAGGGGAGAGGTGTGGCTGACCGACATCAACAATTCCATGCTGACCATTGGTCGCGATCGCCTGCTGGATGA
AGGTACTGCGGTGCCGGTTGCCCAATGCGACGCGGAAAAACTGCCATTCCCTACCAATTACTTCGACTGCGTAAGCGTTG
CTTTTGGTCTGAGGAACATGACGCACAAGGATGTCGCGCTGAAAGAGATGCTGCGCGTATTACGTCCCGGGGGCTGCGTT
ATCGTGCTCGAATTCTCCAGGATATGGAAACCCCTGCAACGGCTCTACGACCTTTACTCGTTCAAGGTGCTTCCCGCGAT
GGGGGGTCTCGTTGCAAAGGACCGGGATAGCTACCGGTATCTTGCCGAATCCATTCGCGTACATCCAAGCCAGGAGGAGT
TGAAGCAAATGATGCAGAAAGCCGGATTCGAGCGCGTGCAATATTTCAATCTTGCTGCAAACGCCGTAGCATTGCACCGC
GGATACAAGTTTTAG

Upstream 100 bases:

>100_bases
GGGTATTTATCGGACTGGAACGGGAAACCAGCGGTAAAAAGCTGAGCTATCCGGAACAAGGCTTCTTCTCCCTTTACCCT
TTATTTAAGTTTGAATATCG

Downstream 100 bases:

>100_bases
GACCCGTTAGCATTGGTTGCGCGCTCGCGACGTATGTCTGCTATTGCACAGAAATCTTTCCGGAGCTGATAATATTGTTG
GTCACTCCGTTTCACAGGGG

Product: ubiquinone/menaquinone biosynthesis methyltransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 244; Mature: 243

Protein sequence:

>244_residues
MTRTTHFGFKTVSETEKAREVAGVFDSVAERYNLMNDLMSGGMHRLWKRFAIEVSGVRTGERVLDIAGGTADLSSLFLEQ
VGSRGEVWLTDINNSMLTIGRDRLLDEGTAVPVAQCDAEKLPFPTNYFDCVSVAFGLRNMTHKDVALKEMLRVLRPGGCV
IVLEFSRIWKPLQRLYDLYSFKVLPAMGGLVAKDRDSYRYLAESIRVHPSQEELKQMMQKAGFERVQYFNLAANAVALHR
GYKF

Sequences:

>Translated_244_residues
MTRTTHFGFKTVSETEKAREVAGVFDSVAERYNLMNDLMSGGMHRLWKRFAIEVSGVRTGERVLDIAGGTADLSSLFLEQ
VGSRGEVWLTDINNSMLTIGRDRLLDEGTAVPVAQCDAEKLPFPTNYFDCVSVAFGLRNMTHKDVALKEMLRVLRPGGCV
IVLEFSRIWKPLQRLYDLYSFKVLPAMGGLVAKDRDSYRYLAESIRVHPSQEELKQMMQKAGFERVQYFNLAANAVALHR
GYKF
>Mature_243_residues
TRTTHFGFKTVSETEKAREVAGVFDSVAERYNLMNDLMSGGMHRLWKRFAIEVSGVRTGERVLDIAGGTADLSSLFLEQV
GSRGEVWLTDINNSMLTIGRDRLLDEGTAVPVAQCDAEKLPFPTNYFDCVSVAFGLRNMTHKDVALKEMLRVLRPGGCVI
VLEFSRIWKPLQRLYDLYSFKVLPAMGGLVAKDRDSYRYLAESIRVHPSQEELKQMMQKAGFERVQYFNLAANAVALHRG
YKF

Specific function: Methyltransferase required for the conversion of dimethylmenaquinone (DMKH2) to menaquinone (MKH2) and the conversion of 2-polyprenyl-6-methoxy-1,4-benzoquinol (DDMQH2) to 2-polyprenyl-3-methyl-6-methoxy-1,4-benzoquinol (DMQH2)

COG id: COG2226

COG function: function code H; Methylase involved in ubiquinone/menaquinone biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. UbiE family

Homologues:

Organism=Homo sapiens, GI116063536, Length=276, Percent_Identity=43.4782608695652, Blast_Score=221, Evalue=6e-58,
Organism=Escherichia coli, GI48994983, Length=244, Percent_Identity=61.4754098360656, Blast_Score=307, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17557039, Length=245, Percent_Identity=41.2244897959184, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6323526, Length=258, Percent_Identity=39.922480620155, Blast_Score=165, Evalue=7e-42,
Organism=Drosophila melanogaster, GI24641702, Length=258, Percent_Identity=45.7364341085271, Blast_Score=222, Evalue=1e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): UBIE_NITMU (Q2Y6R0)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412953.1
- ProteinModelPortal:   Q2Y6R0
- SMR:   Q2Y6R0
- STRING:   Q2Y6R0
- GeneID:   3785431
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2269
- eggNOG:   COG2226
- HOGENOM:   HBG559580
- OMA:   FDVYTIA
- PhylomeDB:   Q2Y6R0
- ProtClustDB:   CLSK585637
- BioCyc:   NMUL323848:NMUL_A2269-MONOMER
- HAMAP:   MF_01813
- InterPro:   IPR004033
- PANTHER:   PTHR10108:SF24
- TIGRFAMs:   TIGR01934

Pfam domain/function: PF01209 Ubie_methyltran

EC number: 2.1.1.-

Molecular weight: Translated: 27598; Mature: 27467

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS01183 UBIE_1; PS01184 UBIE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRTTHFGFKTVSETEKAREVAGVFDSVAERYNLMNDLMSGGMHRLWKRFAIEVSGVRTG
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
ERVLDIAGGTADLSSLFLEQVGSRGEVWLTDINNSMLTIGRDRLLDEGTAVPVAQCDAEK
HHHHEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHCCCCCCCHHHCCCCC
LPFPTNYFDCVSVAFGLRNMTHKDVALKEMLRVLRPGGCVIVLEFSRIWKPLQRLYDLYS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHH
FKVLPAMGGLVAKDRDSYRYLAESIRVHPSQEELKQMMQKAGFERVQYFNLAANAVALHR
HHHHHHHCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GYKF
CCCC
>Mature Secondary Structure 
TRTTHFGFKTVSETEKAREVAGVFDSVAERYNLMNDLMSGGMHRLWKRFAIEVSGVRTG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCC
ERVLDIAGGTADLSSLFLEQVGSRGEVWLTDINNSMLTIGRDRLLDEGTAVPVAQCDAEK
HHHHEECCCHHHHHHHHHHHCCCCCCEEEEECCCCEEEHHHHHHHCCCCCCCHHHCCCCC
LPFPTNYFDCVSVAFGLRNMTHKDVALKEMLRVLRPGGCVIVLEFSRIWKPLQRLYDLYS
CCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHHHH
FKVLPAMGGLVAKDRDSYRYLAESIRVHPSQEELKQMMQKAGFERVQYFNLAANAVALHR
HHHHHHHCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHC
GYKF
CCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring one-carbon groups; Methyltransferases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA