Click here to switch to the map view.

The map label for this gene is rumA

Identifier: 82703334

GI number: 82703334

Start: 2515783

End: 2517102

Strand: Reverse

Name: rumA

Synonym: Nmul_A2216

Alternate gene names: 82703334

Gene position: 2517102-2515783 (Counterclockwise)

Preceding gene: 82703335

Following gene: 82703332

Centisome position: 79.05

GC content: 58.03

Gene sequence:

>1320_bases
ATGACTGCTCCCGTTCTTATCGAATCCCTGGATCAGGAAGGCCGGGGCGTTGCGCATGCCGAAGGCAAGGTCATTTTCAT
CGAGGGCGCCCTGCCGGGAGAAGTCGTCACCTATAACGCCTATCGCAGAAAACCGAGCTTCGAACTTGCCCAGGTCGGTC
AGATTCTCAAGCCTGCGTTTTCGCGGGTAACGCCGGAATGCCACCATTTCGGCATCTGCGGCGGTTGCAGCATGCAGCAT
ATGGACGTTCGCACCCAGGTGGCGGCAAAACAGCGCGTGCTCGAAGATAACCTCAAGCACATCGGCAAGGTTGCACCCGA
ACTGGTATTGCCGGCGGTTTATGGATCACCCTGGGGCTACCGCTACCGGGCACGGTTATCAGTCCGGTATGTGGCAAAAA
AAGGCGGCGTACTGGTCGGTTTTCATGAAAAGCGCAGCAGCTTCATTGCCGACATGCAGGCCTGCAGGATCATGCCGCCG
CGTATTTCCGCCCTCATCATGCCATTACGAAAGCTGGTGGAAAGCCTGTCCATCCGCGAACGTCTGCCGCAGATTGAAGT
TTCACTGGGCGAGGACGTGGATGTACTGGTCCTGCGCATTCTCGAACCCCTGACCGCGCAGGATGAAACCTTGCTCAAGG
CTTTTGCCGACCAGCATCACGTTCAGCTTTTCCTGCAAACCGGCGGTCCTCAAACAGCTTATCCTTTCTATCCCGAAGAC
GCCCCGGAACTGAGTTATACGCTGCCGGAATTCGATGTAACCATTCCGTTTCATCCGACTGAGTTCACCCAGGTGAATCC
GATGGTCAATCGCATACTGGTGCGGCGAGCGCTCAACCTGCTCGATCCCCGGCGGGGAGAACGCATCGCGGACCTGTTTT
GCGGGCTGGGTAATTTCACTCTTCCCGTTGCGCGGCGCGGCGCGCAGGTAGTGGGCTATGAAGGCAGCGCGGCGCTTGTC
TCCCGCGCCCGTCAAAATTCACAACGCAACGGCCTCGCGGGGAGTACGCGATTCATGGAAGCCAACCTGTTTGAAATCGA
TGAAAGTTGGATGCGTGAGCAGGGGGATTTCGACAAGATGCTGATCGATCCGCCGCGGGAGGGTGCTGTTGCCGTGGTGA
CCGCCCTCGAAGAGAAACAGCTCAAGCCCTGGCGCATCGTTTACGTCTCCTGCAATCCCGCCACACTGGCTCGCGACGCG
AGCGTTCTGGTGCACAGGAATGGGTATGCGTTGAAAGCGGCGGGTGTCATCAACATGTTTCCGCATACCGCGCATGTGGA
GTCCATCGCGCTGTTCGAGAAAGCCCGCAGGCAAGCTTGA

Upstream 100 bases:

>100_bases
TTCTCCTGCATGGAGGTTCGGCAGATTCCGGAAAGCATCCGAGGGTAGAATATCCAATCCTTCTGAACCCGTTCCCGATT
TCGAACCTGCCAGTGTGAAT

Downstream 100 bases:

>100_bases
GAGGCATGAAAAAAGGCGGCTGTTTCCAGCCGCCCCGGAGGTATTTCCGGATTCCCCTGGCTTGCTCCTGGGGCTTATCC
TATAGTTTTTCCTATGGCGC

Product: 23S rRNA 5-methyluridine methyltransferase

Products: NA

Alternate protein names: 23S rRNA(M-5-U1939)-methyltransferase

Number of amino acids: Translated: 439; Mature: 438

Protein sequence:

>439_residues
MTAPVLIESLDQEGRGVAHAEGKVIFIEGALPGEVVTYNAYRRKPSFELAQVGQILKPAFSRVTPECHHFGICGGCSMQH
MDVRTQVAAKQRVLEDNLKHIGKVAPELVLPAVYGSPWGYRYRARLSVRYVAKKGGVLVGFHEKRSSFIADMQACRIMPP
RISALIMPLRKLVESLSIRERLPQIEVSLGEDVDVLVLRILEPLTAQDETLLKAFADQHHVQLFLQTGGPQTAYPFYPED
APELSYTLPEFDVTIPFHPTEFTQVNPMVNRILVRRALNLLDPRRGERIADLFCGLGNFTLPVARRGAQVVGYEGSAALV
SRARQNSQRNGLAGSTRFMEANLFEIDESWMREQGDFDKMLIDPPREGAVAVVTALEEKQLKPWRIVYVSCNPATLARDA
SVLVHRNGYALKAAGVINMFPHTAHVESIALFEKARRQA

Sequences:

>Translated_439_residues
MTAPVLIESLDQEGRGVAHAEGKVIFIEGALPGEVVTYNAYRRKPSFELAQVGQILKPAFSRVTPECHHFGICGGCSMQH
MDVRTQVAAKQRVLEDNLKHIGKVAPELVLPAVYGSPWGYRYRARLSVRYVAKKGGVLVGFHEKRSSFIADMQACRIMPP
RISALIMPLRKLVESLSIRERLPQIEVSLGEDVDVLVLRILEPLTAQDETLLKAFADQHHVQLFLQTGGPQTAYPFYPED
APELSYTLPEFDVTIPFHPTEFTQVNPMVNRILVRRALNLLDPRRGERIADLFCGLGNFTLPVARRGAQVVGYEGSAALV
SRARQNSQRNGLAGSTRFMEANLFEIDESWMREQGDFDKMLIDPPREGAVAVVTALEEKQLKPWRIVYVSCNPATLARDA
SVLVHRNGYALKAAGVINMFPHTAHVESIALFEKARRQA
>Mature_438_residues
TAPVLIESLDQEGRGVAHAEGKVIFIEGALPGEVVTYNAYRRKPSFELAQVGQILKPAFSRVTPECHHFGICGGCSMQHM
DVRTQVAAKQRVLEDNLKHIGKVAPELVLPAVYGSPWGYRYRARLSVRYVAKKGGVLVGFHEKRSSFIADMQACRIMPPR
ISALIMPLRKLVESLSIRERLPQIEVSLGEDVDVLVLRILEPLTAQDETLLKAFADQHHVQLFLQTGGPQTAYPFYPEDA
PELSYTLPEFDVTIPFHPTEFTQVNPMVNRILVRRALNLLDPRRGERIADLFCGLGNFTLPVARRGAQVVGYEGSAALVS
RARQNSQRNGLAGSTRFMEANLFEIDESWMREQGDFDKMLIDPPREGAVAVVTALEEKQLKPWRIVYVSCNPATLARDAS
VLVHRNGYALKAAGVINMFPHTAHVESIALFEKARRQA

Specific function: Catalyzes the formation of 5-methyl-uridine at position 1939 (M-5-U1939) in 23S rRNA

COG id: COG2265

COG function: function code J; SAM-dependent methyltransferases related to tRNA (uracil-5-)-methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Escherichia coli, GI1789148, Length=437, Percent_Identity=38.4439359267735, Blast_Score=282, Evalue=4e-77,
Organism=Escherichia coli, GI1787083, Length=191, Percent_Identity=30.8900523560209, Blast_Score=80, Evalue=3e-16,
Organism=Escherichia coli, GI1790403, Length=178, Percent_Identity=29.2134831460674, Blast_Score=69, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24666579, Length=196, Percent_Identity=29.5918367346939, Blast_Score=86, Evalue=7e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUMA_NITMU (Q2Y6W3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412900.1
- ProteinModelPortal:   Q2Y6W3
- SMR:   Q2Y6W3
- STRING:   Q2Y6W3
- GeneID:   3786300
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2216
- eggNOG:   COG2265
- HOGENOM:   HBG690163
- OMA:   VVFVEGA
- PhylomeDB:   Q2Y6W3
- ProtClustDB:   PRK13168
- BioCyc:   NMUL323848:NMUL_A2216-MONOMER
- HAMAP:   MF_01010
- InterPro:   IPR016027
- InterPro:   IPR001566
- InterPro:   IPR002792
- InterPro:   IPR010280
- TIGRFAMs:   TIGR00479

Pfam domain/function: PF01938 TRAM; PF05958 tRNA_U5-meth_tr; SSF50249 Nucleic_acid_OB

EC number: =2.1.1.190

Molecular weight: Translated: 48959; Mature: 48828

Theoretical pI: Translated: 8.56; Mature: 8.56

Prosite motif: PS50926 TRAM; PS01230 TRMA_1; PS01231 TRMA_2

Important sites: ACT_SITE 391-391 BINDING 264-264 BINDING 293-293 BINDING 298-298 BINDING 314-314 BINDING 342-342 BINDING 363-363

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAPVLIESLDQEGRGVAHAEGKVIFIEGALPGEVVTYNAYRRKPSFELAQVGQILKPAF
CCCCHHHHHHCCCCCCEEECCCCEEEEECCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHH
SRVTPECHHFGICGGCSMQHMDVRTQVAAKQRVLEDNLKHIGKVAPELVLPAVYGSPWGY
HHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RYRARLSVRYVAKKGGVLVGFHEKRSSFIADMQACRIMPPRISALIMPLRKLVESLSIRE
EEEEEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLPQIEVSLGEDVDVLVLRILEPLTAQDETLLKAFADQHHVQLFLQTGGPQTAYPFYPED
HCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
APELSYTLPEFDVTIPFHPTEFTQVNPMVNRILVRRALNLLDPRRGERIADLFCGLGNFT
CCCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCE
LPVARRGAQVVGYEGSAALVSRARQNSQRNGLAGSTRFMEANLFEIDESWMREQGDFDKM
EHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHCCHHHHHCCCCCCCE
LIDPPREGAVAVVTALEEKQLKPWRIVYVSCNPATLARDASVLVHRNGYALKAAGVINMF
EECCCCCCCEEEEEEHHHCCCCCEEEEEEECCCHHHCCCCEEEEECCCEEEEECCEEEEC
PHTAHVESIALFEKARRQA
CCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TAPVLIESLDQEGRGVAHAEGKVIFIEGALPGEVVTYNAYRRKPSFELAQVGQILKPAF
CCCHHHHHHCCCCCCEEECCCCEEEEECCCCCCEEEEHHHHCCCCCCHHHHHHHHHHHH
SRVTPECHHFGICGGCSMQHMDVRTQVAAKQRVLEDNLKHIGKVAPELVLPAVYGSPWGY
HHCCCCHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCE
RYRARLSVRYVAKKGGVLVGFHEKRSSFIADMQACRIMPPRISALIMPLRKLVESLSIRE
EEEEEEEEEEEECCCCEEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
RLPQIEVSLGEDVDVLVLRILEPLTAQDETLLKAFADQHHVQLFLQTGGPQTAYPFYPED
HCCCEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCC
APELSYTLPEFDVTIPFHPTEFTQVNPMVNRILVRRALNLLDPRRGERIADLFCGLGNFT
CCCCEEECCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCE
LPVARRGAQVVGYEGSAALVSRARQNSQRNGLAGSTRFMEANLFEIDESWMREQGDFDKM
EHHHHCCCEEEECCCCHHHHHHHHHHHHCCCCCCCCEEEECHHHHCCHHHHHCCCCCCCE
LIDPPREGAVAVVTALEEKQLKPWRIVYVSCNPATLARDASVLVHRNGYALKAAGVINMF
EECCCCCCCEEEEEEHHHCCCCCEEEEEEECCCHHHCCCCEEEEECCCEEEEECCEEEEC
PHTAHVESIALFEKARRQA
CCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA