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The map label for this gene is gyrA [H]

Identifier: 82703307

GI number: 82703307

Start: 2485260

End: 2487941

Strand: Direct

Name: gyrA [H]

Synonym: Nmul_A2189

Alternate gene names: 82703307

Gene position: 2485260-2487941 (Clockwise)

Preceding gene: 82703293

Following gene: 82703308

Centisome position: 78.05

GC content: 56.75

Gene sequence:

>2682_bases
ATGCTAAAATGGGGGGCTTTATATGCCGATATCAGGAAAGAAATATATCCTCGATATGTTCCCGTTCCCGCAAGTTCTTG
TCCCCCTTCAAAAGTAATGGATCAATTCGCGAAAGAAACCCTGCAGATCAGTCTCGAAGAGGAAATGCGCCGCTCCTATC
TCGACTACGCCATGAGCGTCATCGTGGGACGCGCATTACCCGACGTGCGCGACGGCCTGAAACCGGTGCATCGCCGGGTT
CTATTCGCTATGCATGAACTCTCCAACGACTGGAACCGTCCCTACAAAAAATCGGCGCGCGTCGTCGGTGATGTCATCGG
TAAATATCACCCGCACGGCGATACTGCCGTATATGACACTATAGTACGGATGGCGCAGAATTTCTCGCTGCGCTACATGC
TCGTGGATGGACAGGGCAACTTCGGCTCCGTGGATGGCGACAACCCGGCGGCAATGCGTTATACCGAAATCCGCATGTCT
CGCATCGCGCATGAAATGCTGGCCGATCTGGATAAGGAAACGGTTGATTTCGGCCCTAACTATGACGGCACCGAGAAAGA
ACCGCTGATCCTGCCGACCAAAATCCCGAACCTGCTTATCAATGGTTCGTCCGGGATTGCGGTGGGCATGGCAACCAATA
TTCCGCCACATAACCTGAATGAAGTGACGGATGCCTGCCTCGCCCTGCTGAAGAACCCCGAAATCGCCATCGAGGATCTG
ATCGAACTGATACCGGGCCCGGATTTTCCCACCGCCGCGCTGATCTATGGCGTGACCGGCGTAAAAGAAGGCTACCGCAC
GGGCCGCGGGCGCGTAATCATGCGCGCGCGCACTCACTTCGAGGATATGGAAAAAGGGGGCGGACGCCAGAGCATCGTTA
TCGACGAGCTGCCCTACCAGGTAAACAAGGCCAATCTGCTTATCCGTATCGGCGAGTTGGTGCGGGATAAGAAAATCGAA
GGCATTTCCGATTTGCGCGATGAATCGGATAAATCGGGGATGCGCGTGGTTATCGAACTGCGCCGCAACGAAGTACCCGA
GGTGGTGCTGAACAATCTCTATAAAGAAACGCAAATGCAGGACACCTTCGGCATGAACATGGTGGCCCTGCTTGACGGGC
AGCCGCGGCTACTGAATTTGAAGCAGATGCTGGAGGCGTTCTTGCGCCACCGGCGCGAGGTCGTTACGCGGCGCAGCGTA
TTCGAGTTGAAAAAGGCCCGTGAACGCGGCCATGTGCTGGAGGGACTCGCTGTCGCCTTATCCAACGTGGATGAAGTGAT
TGCCCTGATCAAGGCAGCGCAGACGCCTGCAGCAGCCAAGGAAGCATTGATGTCGCGGGTGTGGCGCTCGCGACTGGTGG
AGCAGATGCTGGCACGCGCTGCAGTGGATGCGAAGCAATTCCGACCCGAGGGCCTGGCGCCCGAATACGGGTTATTTGAT
GACGGCTATCATCTTTCCGATGGACAGGCACAAGCTATACTGGAATTGCGCCTGCAACGCCTGACGGGGCTCGAGCAGGA
CAAGATTGTCAGCGAGTATAGGGATGTGATGGAGAGAATAGCCGACCTTCTCGACATTCTTGCCCGCCCGGAGCGTATCA
CGGCCATCATCACCGAGGAGCTTGTTGCGATGAAGCAACAGTTTGGTGATAAACGCCGCAGCGAGATCGTCATCAATACT
CAGGATTTGAGCATGGAGGACCTGATCGCATCCGAGGATGTGGTCGTGACGCTATCGCACAATGGATACATCAAATCCCA
GCCGCTCGACGACTATCGCGCGCAGAAACGGGGCGGCCGGGGGAAGCAGGCAACAGGCACCAAAGATGATGATTTCATCG
ATAACCTGTTTATCGCCAATACGCACGACTATATTCTCTGCTTTTCCAGCCGGGGGAGGGTCTACTGGATCAAGGTGTAT
TCCGTGCCGCAGGGAGGACGCGCTTCCCGCGGCAGGCCTATCGTCAACCTGATGCAACTGGAATACGGCGAGAAGATCAA
TGCCGTTCTTCCGATAAAGACATTCGATGACAACCGTTATGTATTCATGGCAACTTCTCTGGGCACGGTGAAAAAAACAC
CCCTGTCGGAGTTTTCGCGGCCGCGCGCGAGTGGCATCATAGCGGTAGGTCTCGACGAGGATGATTTCCTGATCGGCGTA
GCGCTGACCGAGGGCAAGCATGATGTGATGCTCTTCTCCGATGGAGGGAAAGCCGTGCGTTTCGATGAGAACGACGTACG
CCCGATGGGACGGGGCGCGCGGGGTGTGCGCGGGATGACGCTGGGCAAGGGGCAAAAGGTGATTTCGCTGCTGGTGGCCG
AGAGCGAGGAGCAGGCGGTGCTGACCGCAACCGAGAATGGCTATGGCAAACGTACTCCCATCAGCGAATACACTCGTCAC
GGCCGGGGTTCGCAGGGCATGATTGCGATCAAGACCACCCAGCGCAACGGCAAGGTCGTGGGGGCGCGCCTGGTGCGCGA
GGACGACGAAATCATGCTCATCACGACCGGTGGCGTGCTGATCCGCACACGCGTGAAAGAGGTGCGCGAGATGAGCCGGG
CAACACAGGGCGTGATACTGATCAACCTGGATGAGGGTGAGAAGCTTGCCGGGCTGGAAAGGGTCGTGGAAACTGACGAA
GCGCCAGGATTAGGGGAAGGCTTAGCCACGGATTTGCCGTGA

Upstream 100 bases:

>100_bases
TCGCGCTTTGATTATGTATTCATATATTTGTCCTTGTCAAATAGGGGTGTTGCAATCGGCATGAAAACCGGGCTTTCCGG
GTGACTCCCCTACGGGCGGC

Downstream 100 bases:

>100_bases
ACAGCCAGAGTAAGAACTCATGAGGAATTCATAAGGAACTCCGATGGAACCCATCTATAACTTCAGCGCAGGTCCGGCGG
TATTACCAAAGGAAGTTTTG

Product: DNA gyrase subunit A

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 893; Mature: 893

Protein sequence:

>893_residues
MLKWGALYADIRKEIYPRYVPVPASSCPPSKVMDQFAKETLQISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRRV
LFAMHELSNDWNRPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYMLVDGQGNFGSVDGDNPAAMRYTEIRMS
RIAHEMLADLDKETVDFGPNYDGTEKEPLILPTKIPNLLINGSSGIAVGMATNIPPHNLNEVTDACLALLKNPEIAIEDL
IELIPGPDFPTAALIYGVTGVKEGYRTGRGRVIMRARTHFEDMEKGGGRQSIVIDELPYQVNKANLLIRIGELVRDKKIE
GISDLRDESDKSGMRVVIELRRNEVPEVVLNNLYKETQMQDTFGMNMVALLDGQPRLLNLKQMLEAFLRHRREVVTRRSV
FELKKARERGHVLEGLAVALSNVDEVIALIKAAQTPAAAKEALMSRVWRSRLVEQMLARAAVDAKQFRPEGLAPEYGLFD
DGYHLSDGQAQAILELRLQRLTGLEQDKIVSEYRDVMERIADLLDILARPERITAIITEELVAMKQQFGDKRRSEIVINT
QDLSMEDLIASEDVVVTLSHNGYIKSQPLDDYRAQKRGGRGKQATGTKDDDFIDNLFIANTHDYILCFSSRGRVYWIKVY
SVPQGGRASRGRPIVNLMQLEYGEKINAVLPIKTFDDNRYVFMATSLGTVKKTPLSEFSRPRASGIIAVGLDEDDFLIGV
ALTEGKHDVMLFSDGGKAVRFDENDVRPMGRGARGVRGMTLGKGQKVISLLVAESEEQAVLTATENGYGKRTPISEYTRH
GRGSQGMIAIKTTQRNGKVVGARLVREDDEIMLITTGGVLIRTRVKEVREMSRATQGVILINLDEGEKLAGLERVVETDE
APGLGEGLATDLP

Sequences:

>Translated_893_residues
MLKWGALYADIRKEIYPRYVPVPASSCPPSKVMDQFAKETLQISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRRV
LFAMHELSNDWNRPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYMLVDGQGNFGSVDGDNPAAMRYTEIRMS
RIAHEMLADLDKETVDFGPNYDGTEKEPLILPTKIPNLLINGSSGIAVGMATNIPPHNLNEVTDACLALLKNPEIAIEDL
IELIPGPDFPTAALIYGVTGVKEGYRTGRGRVIMRARTHFEDMEKGGGRQSIVIDELPYQVNKANLLIRIGELVRDKKIE
GISDLRDESDKSGMRVVIELRRNEVPEVVLNNLYKETQMQDTFGMNMVALLDGQPRLLNLKQMLEAFLRHRREVVTRRSV
FELKKARERGHVLEGLAVALSNVDEVIALIKAAQTPAAAKEALMSRVWRSRLVEQMLARAAVDAKQFRPEGLAPEYGLFD
DGYHLSDGQAQAILELRLQRLTGLEQDKIVSEYRDVMERIADLLDILARPERITAIITEELVAMKQQFGDKRRSEIVINT
QDLSMEDLIASEDVVVTLSHNGYIKSQPLDDYRAQKRGGRGKQATGTKDDDFIDNLFIANTHDYILCFSSRGRVYWIKVY
SVPQGGRASRGRPIVNLMQLEYGEKINAVLPIKTFDDNRYVFMATSLGTVKKTPLSEFSRPRASGIIAVGLDEDDFLIGV
ALTEGKHDVMLFSDGGKAVRFDENDVRPMGRGARGVRGMTLGKGQKVISLLVAESEEQAVLTATENGYGKRTPISEYTRH
GRGSQGMIAIKTTQRNGKVVGARLVREDDEIMLITTGGVLIRTRVKEVREMSRATQGVILINLDEGEKLAGLERVVETDE
APGLGEGLATDLP
>Mature_893_residues
MLKWGALYADIRKEIYPRYVPVPASSCPPSKVMDQFAKETLQISLEEEMRRSYLDYAMSVIVGRALPDVRDGLKPVHRRV
LFAMHELSNDWNRPYKKSARVVGDVIGKYHPHGDTAVYDTIVRMAQNFSLRYMLVDGQGNFGSVDGDNPAAMRYTEIRMS
RIAHEMLADLDKETVDFGPNYDGTEKEPLILPTKIPNLLINGSSGIAVGMATNIPPHNLNEVTDACLALLKNPEIAIEDL
IELIPGPDFPTAALIYGVTGVKEGYRTGRGRVIMRARTHFEDMEKGGGRQSIVIDELPYQVNKANLLIRIGELVRDKKIE
GISDLRDESDKSGMRVVIELRRNEVPEVVLNNLYKETQMQDTFGMNMVALLDGQPRLLNLKQMLEAFLRHRREVVTRRSV
FELKKARERGHVLEGLAVALSNVDEVIALIKAAQTPAAAKEALMSRVWRSRLVEQMLARAAVDAKQFRPEGLAPEYGLFD
DGYHLSDGQAQAILELRLQRLTGLEQDKIVSEYRDVMERIADLLDILARPERITAIITEELVAMKQQFGDKRRSEIVINT
QDLSMEDLIASEDVVVTLSHNGYIKSQPLDDYRAQKRGGRGKQATGTKDDDFIDNLFIANTHDYILCFSSRGRVYWIKVY
SVPQGGRASRGRPIVNLMQLEYGEKINAVLPIKTFDDNRYVFMATSLGTVKKTPLSEFSRPRASGIIAVGLDEDDFLIGV
ALTEGKHDVMLFSDGGKAVRFDENDVRPMGRGARGVRGMTLGKGQKVISLLVAESEEQAVLTATENGYGKRTPISEYTRH
GRGSQGMIAIKTTQRNGKVVGARLVREDDEIMLITTGGVLIRTRVKEVREMSRATQGVILINLDEGEKLAGLERVVETDE
APGLGEGLATDLP

Specific function: DNA gyrase negatively supercoils closed circular double- stranded DNA in an ATP-dependent manner and also catalyzes the interconversion of other topological isomers of double-stranded DNA rings, including catenanes and knotted rings [H]

COG id: COG0188

COG function: function code L; Type IIA topoisomerase (DNA gyrase/topo II, topoisomerase IV), A subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the topoisomerase gyrA/parC subunit family [H]

Homologues:

Organism=Homo sapiens, GI19913408, Length=320, Percent_Identity=27.5, Blast_Score=81, Evalue=6e-15,
Organism=Homo sapiens, GI19913406, Length=320, Percent_Identity=26.25, Blast_Score=77, Evalue=9e-14,
Organism=Escherichia coli, GI1788562, Length=845, Percent_Identity=61.8934911242604, Blast_Score=1073, Evalue=0.0,
Organism=Escherichia coli, GI1789396, Length=742, Percent_Identity=35.44474393531, Blast_Score=411, Evalue=1e-116,
Organism=Caenorhabditis elegans, GI17535065, Length=356, Percent_Identity=25, Blast_Score=73, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI212645657, Length=385, Percent_Identity=24.1558441558442, Blast_Score=72, Evalue=1e-12,
Organism=Caenorhabditis elegans, GI212645845, Length=189, Percent_Identity=29.1005291005291, Blast_Score=67, Evalue=6e-11,
Organism=Saccharomyces cerevisiae, GI6324241, Length=399, Percent_Identity=24.812030075188, Blast_Score=76, Evalue=2e-14,
Organism=Drosophila melanogaster, GI17136538, Length=410, Percent_Identity=25.609756097561, Blast_Score=81, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020899
- InterPro:   IPR005743
- InterPro:   IPR006691
- InterPro:   IPR002205
- InterPro:   IPR013758
- InterPro:   IPR013757
- InterPro:   IPR013760 [H]

Pfam domain/function: PF03989 DNA_gyraseA_C; PF00521 DNA_topoisoIV [H]

EC number: =5.99.1.3 [H]

Molecular weight: Translated: 99665; Mature: 99665

Theoretical pI: Translated: 6.54; Mature: 6.54

Prosite motif: PS00018 EF_HAND_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKWGALYADIRKEIYPRYVPVPASSCPPSKVMDQFAKETLQISLEEEMRRSYLDYAMSV
CCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHH
IVGRALPDVRDGLKPVHRRVLFAMHELSNDWNRPYKKSARVVGDVIGKYHPHGDTAVYDT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
IVRMAQNFSLRYMLVDGQGNFGSVDGDNPAAMRYTEIRMSRIAHEMLADLDKETVDFGPN
HHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
YDGTEKEPLILPTKIPNLLINGSSGIAVGMATNIPPHNLNEVTDACLALLKNPEIAIEDL
CCCCCCCCEEECCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHH
IELIPGPDFPTAALIYGVTGVKEGYRTGRGRVIMRARTHFEDMEKGGGRQSIVIDELPYQ
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCE
VNKANLLIRIGELVRDKKIEGISDLRDESDKSGMRVVIELRRNEVPEVVLNNLYKETQMQ
ECCCCEEEEEHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHH
DTFGMNMVALLDGQPRLLNLKQMLEAFLRHRREVVTRRSVFELKKARERGHVLEGLAVAL
HHHCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
SNVDEVIALIKAAQTPAAAKEALMSRVWRSRLVEQMLARAAVDAKQFRPEGLAPEYGLFD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DGYHLSDGQAQAILELRLQRLTGLEQDKIVSEYRDVMERIADLLDILARPERITAIITEE
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LVAMKQQFGDKRRSEIVINTQDLSMEDLIASEDVVVTLSHNGYIKSQPLDDYRAQKRGGR
HHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCEEEEEECCCEEECCCCHHHHHHHCCCC
GKQATGTKDDDFIDNLFIANTHDYILCFSSRGRVYWIKVYSVPQGGRASRGRPIVNLMQL
CCCCCCCCCCHHHHCEEEECCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
EYGEKINAVLPIKTFDDNRYVFMATSLGTVKKTPLSEFSRPRASGIIAVGLDEDDFLIGV
HCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCCCEEEEE
ALTEGKHDVMLFSDGGKAVRFDENDVRPMGRGARGVRGMTLGKGQKVISLLVAESEEQAV
EEECCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEE
LTATENGYGKRTPISEYTRHGRGSQGMIAIKTTQRNGKVVGARLVREDDEIMLITTGGVL
EEEECCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEEECCEE
IRTRVKEVREMSRATQGVILINLDEGEKLAGLERVVETDEAPGLGEGLATDLP
EHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MLKWGALYADIRKEIYPRYVPVPASSCPPSKVMDQFAKETLQISLEEEMRRSYLDYAMSV
CCCCHHHHHHHHHHHCCCEECCCCCCCCHHHHHHHHHHHHHEEEHHHHHHHHHHHHHHHH
IVGRALPDVRDGLKPVHRRVLFAMHELSNDWNRPYKKSARVVGDVIGKYHPHGDTAVYDT
HHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCHHHHHH
IVRMAQNFSLRYMLVDGQGNFGSVDGDNPAAMRYTEIRMSRIAHEMLADLDKETVDFGPN
HHHHHHCCCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCHHHHCCCCC
YDGTEKEPLILPTKIPNLLINGSSGIAVGMATNIPPHNLNEVTDACLALLKNPEIAIEDL
CCCCCCCCEEECCCCCCEEEECCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHH
IELIPGPDFPTAALIYGVTGVKEGYRTGRGRVIMRARTHFEDMEKGGGRQSIVIDELPYQ
HHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCEEEEECCCCE
VNKANLLIRIGELVRDKKIEGISDLRDESDKSGMRVVIELRRNEVPEVVLNNLYKETQMQ
ECCCCEEEEEHHHHHHHCCCCHHHHHCCCCCCCCEEEEEEHHCCCHHHHHHHHHHHHHHH
DTFGMNMVALLDGQPRLLNLKQMLEAFLRHRREVVTRRSVFELKKARERGHVLEGLAVAL
HHHCCCEEEEECCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHH
SNVDEVIALIKAAQTPAAAKEALMSRVWRSRLVEQMLARAAVDAKQFRPEGLAPEYGLFD
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
DGYHLSDGQAQAILELRLQRLTGLEQDKIVSEYRDVMERIADLLDILARPERITAIITEE
CCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
LVAMKQQFGDKRRSEIVINTQDLSMEDLIASEDVVVTLSHNGYIKSQPLDDYRAQKRGGR
HHHHHHHHCCCCCCEEEEEECCCCHHHHHCCCCEEEEEECCCEEECCCCHHHHHHHCCCC
GKQATGTKDDDFIDNLFIANTHDYILCFSSRGRVYWIKVYSVPQGGRASRGRPIVNLMQL
CCCCCCCCCCHHHHCEEEECCCCEEEEECCCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
EYGEKINAVLPIKTFDDNRYVFMATSLGTVKKTPLSEFSRPRASGIIAVGLDEDDFLIGV
HCCCCCCEEEEEEEECCCEEEEEEECCCCCCCCCHHHHCCCCCCCEEEEECCCCCEEEEE
ALTEGKHDVMLFSDGGKAVRFDENDVRPMGRGARGVRGMTLGKGQKVISLLVAESEEQAV
EEECCCCEEEEECCCCCEEEECCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEE
LTATENGYGKRTPISEYTRHGRGSQGMIAIKTTQRNGKVVGARLVREDDEIMLITTGGVL
EEEECCCCCCCCCHHHHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEEEEECCEE
IRTRVKEVREMSRATQGVILINLDEGEKLAGLERVVETDEAPGLGEGLATDLP
EHHHHHHHHHHHHHHCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7811002; 10984043 [H]