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The map label for this gene is gph2 [H]

Identifier: 82703303

GI number: 82703303

Start: 2481462

End: 2482109

Strand: Reverse

Name: gph2 [H]

Synonym: Nmul_A2185

Alternate gene names: 82703303

Gene position: 2482109-2481462 (Counterclockwise)

Preceding gene: 82703304

Following gene: 82703302

Centisome position: 77.95

GC content: 62.35

Gene sequence:

>648_bases
ATGATTAACGCCGTTCTCTTCGATCTCGACGGAACGCTTGCCGATACCGCCCCCGATCTCGGGTATGCCCTGAATCGGCA
GCGCATCGCCCGCGGGCTGCTGCCCCTGCCGTTGGAGCTCATCCGTACCGAGGCTTCCGCGGGGGCGCGCGGCCTGTTGG
GCCTGGGATTCAACATCAAGCCCGGGGACGCCGGATATGACGCCATGCGCACCGAATTCCTCGACTTCTACGCGGAACAC
CTCTGCCGCGAGACATTCTTGTTCGCAGGGGTCGCGGATCTCCTCGATCAGCTCGATGACCGGGGCCTGATCTGGGGCAT
CGTCACCAACAAACCCGCTCGTTTTTCCGTACCGCTCCTCGAGGCGCTGGGTTTGGGCAATCGTGCTTCCTGCCTTATCA
GCGGGGGCGATACCACGCACTCCAAACCCCATCCCGAGCCTCTGCTTACAGCGAGCGGAGCAATAGCCGTCCCGCCGGAA
GAATGCATCTATCTGGGCGACGACCTGCGCGACGTCCAGGCCAGCCTCGCCGCCGGCATGGAACCCATCATCGCCAGATA
CGGCTATCTCGGCAATGTAGGTGCCCCCGAAACCTGGGGTGCAAGATACCTCATAGACCGGCCCGAAGAACTGCTCGGCT
ATTTGTAA

Upstream 100 bases:

>100_bases
ACTCCAAGCGATCATTTCAGTTGAGCCCGGTCATCGGTGACATCATCAATCTGGATTGGCGCCCATCTTTATTCCTCCGG
TTCGTATCCATGCTTCTTTC

Downstream 100 bases:

>100_bases
AAACCGTTCGCACTCTCTTTTCTTATTTGCTCGTCTGGAAAAGCCCCTTCCCTGGCAGGGAGAGGGGGGGGAAGGCAGCG
ACGGATTTTCGCCTCGCCCG

Product: phosphoglycolate phosphatase

Products: NA

Alternate protein names: PGP 2; PGPase2 [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MINAVLFDLDGTLADTAPDLGYALNRQRIARGLLPLPLELIRTEASAGARGLLGLGFNIKPGDAGYDAMRTEFLDFYAEH
LCRETFLFAGVADLLDQLDDRGLIWGIVTNKPARFSVPLLEALGLGNRASCLISGGDTTHSKPHPEPLLTASGAIAVPPE
ECIYLGDDLRDVQASLAAGMEPIIARYGYLGNVGAPETWGARYLIDRPEELLGYL

Sequences:

>Translated_215_residues
MINAVLFDLDGTLADTAPDLGYALNRQRIARGLLPLPLELIRTEASAGARGLLGLGFNIKPGDAGYDAMRTEFLDFYAEH
LCRETFLFAGVADLLDQLDDRGLIWGIVTNKPARFSVPLLEALGLGNRASCLISGGDTTHSKPHPEPLLTASGAIAVPPE
ECIYLGDDLRDVQASLAAGMEPIIARYGYLGNVGAPETWGARYLIDRPEELLGYL
>Mature_215_residues
MINAVLFDLDGTLADTAPDLGYALNRQRIARGLLPLPLELIRTEASAGARGLLGLGFNIKPGDAGYDAMRTEFLDFYAEH
LCRETFLFAGVADLLDQLDDRGLIWGIVTNKPARFSVPLLEALGLGNRASCLISGGDTTHSKPHPEPLLTASGAIAVPPE
ECIYLGDDLRDVQASLAAGMEPIIARYGYLGNVGAPETWGARYLIDRPEELLGYL

Specific function: Specifically catalyzes the dephosphorylation of 2- phosphoglycolate. Is involved in the dissimilation of the intracellular 2-phosphoglycolate formed during the DNA repair of 3'-phosphoglycolate ends, a major class of DNA lesions induced by oxidative stres

COG id: COG0546

COG function: function code R; Predicted phosphatases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. CbbY/CbbZ/Gph/YieH family [H]

Homologues:

Organism=Escherichia coli, GI1789787, Length=211, Percent_Identity=35.5450236966825, Blast_Score=108, Evalue=3e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005834
- InterPro:   IPR023214
- InterPro:   IPR006439
- InterPro:   IPR006402
- InterPro:   IPR006346
- InterPro:   IPR023198 [H]

Pfam domain/function: PF00702 Hydrolase [H]

EC number: =3.1.3.18 [H]

Molecular weight: Translated: 23140; Mature: 23140

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINAVLFDLDGTLADTAPDLGYALNRQRIARGLLPLPLELIRTEASAGARGLLGLGFNIK
CCCEEEEECCCCCCCCCCCHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCEEECCCEEC
PGDAGYDAMRTEFLDFYAEHLCRETFLFAGVADLLDQLDDRGLIWGIVTNKPARFSVPLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCHHH
EALGLGNRASCLISGGDTTHSKPHPEPLLTASGAIAVPPEECIYLGDDLRDVQASLAAGM
HHHCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEECCHHHEEEECCCHHHHHHHHHHCH
EPIIARYGYLGNVGAPETWGARYLIDRPEELLGYL
HHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHCCC
>Mature Secondary Structure
MINAVLFDLDGTLADTAPDLGYALNRQRIARGLLPLPLELIRTEASAGARGLLGLGFNIK
CCCEEEEECCCCCCCCCCCHHHHHCHHHHHHCCCCCCHHHHHHHHCCCCCCEEECCCEEC
PGDAGYDAMRTEFLDFYAEHLCRETFLFAGVADLLDQLDDRGLIWGIVTNKPARFSVPLL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCEECCHHH
EALGLGNRASCLISGGDTTHSKPHPEPLLTASGAIAVPPEECIYLGDDLRDVQASLAAGM
HHHCCCCCCEEEEECCCCCCCCCCCCCEEECCCEEECCHHHEEEECCCHHHHHHHHHHCH
EPIIARYGYLGNVGAPETWGARYLIDRPEELLGYL
HHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]