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The map label for this gene is iaaA [H]

Identifier: 82703285

GI number: 82703285

Start: 2458554

End: 2459555

Strand: Reverse

Name: iaaA [H]

Synonym: Nmul_A2166

Alternate gene names: 82703285

Gene position: 2459555-2458554 (Counterclockwise)

Preceding gene: 82703286

Following gene: 82703284

Centisome position: 77.24

GC content: 59.08

Gene sequence:

>1002_bases
ATGACGATACACATGCGCGGAAGCGCGGGGAATGATTCGCCGATCCGGCTCGTGATTCACGGAGGGGCCGGGAGCGTGAA
ATCCGGGCAGGGGCCAGAACGCGAGCAGGCTTACAGGGAGGCACTCGCGCAATCGCTGATCGCTGGCCATGCGGTGCTTG
CAGCAGGCGGCAACAGCATCGATGCAGTGATTGCGGCCATTACAGTGATGGAGGATTGCCCCTTGTTCAATGCCGGCAAG
GGCGCCGTGCTGACCCATGATGGCCGCAACGAACTGGAAGCTTCAATCATGGAAGGGGCCACTCGCGCGGCAGGCGCCGT
TGCGGGAGTGACGACGATTCGCAATCCGATTCGCGCCGCACATGCGGTAATGACGAAAAGCGCCCACGTGATGCTCATCG
GGCAAGGTGCGGAAATTTTCGCTGCAAAGCAGGATCTGGAAATCGTGGATTCGTCCTATTTCTATACCCGGCACCGTTGG
AATCAGCTGCAGAAAGCCATCGCTAAAGAAAGTATCCTGCTCGACCATGATGCGGGTCTGGATACATTGCCGGGTGAGGA
TGAAAAACGTGGAACCGTTGGCGCAGTGGCGCTCGACTGCCAGGGCAATCTGGCGGCCGGCACGTCGACCGGTGGGCTCA
CGAACAAGCACCCTGGCCGGGTAGGAGACTCGTCCATTATCGGGGCGGGAACCTACGCGGACAATCGCTCGGTTGCGGTA
TCCACGACTGGTACAGGCGAAATGTTCATTCGTACCGCTGCCGCTTTCAACACAGCAGCGCAGGTGCGATTTCTGCATGC
TCCGATTACTGCGGCAGCCGATAACACGCTGGAGGAAATCGCGGCGATAGGTGGAGATGGGGGCCTGATCGTCCTGGATG
CGGACGGCAACTATGCGATACGGTTCAACACCGGCGCCATGTTTCGCGGCACAATTGGAGAGGATGGCATAGCACGGACA
GGTATTTTTCCCGGGCCTGAAACACCAGCTCTGTCATGTTGA

Upstream 100 bases:

>100_bases
GATTGGCGGATGGCAGTTGCGTCCGATCCCGTTCTCTACACTCTTCATGCAGGCACTGGCCGCATGACCGGTAATGTCGA
TGCTCTGTAGATCTGTTTAG

Downstream 100 bases:

>100_bases
CAAGCTGCCGCCTTCTGCGAGCCAAATATCGCTGCCGCGTATGAGGATGAACCGGTTACAGCAAAGAGGAGAAAGATAGC
CTATACTTCGAGAGACAGAC

Product: peptidase T2, asparaginase 2

Products: NA

Alternate protein names: Beta-aspartyl-peptidase; EcAIII; Isoaspartyl dipeptidase [H]

Number of amino acids: Translated: 333; Mature: 332

Protein sequence:

>333_residues
MTIHMRGSAGNDSPIRLVIHGGAGSVKSGQGPEREQAYREALAQSLIAGHAVLAAGGNSIDAVIAAITVMEDCPLFNAGK
GAVLTHDGRNELEASIMEGATRAAGAVAGVTTIRNPIRAAHAVMTKSAHVMLIGQGAEIFAAKQDLEIVDSSYFYTRHRW
NQLQKAIAKESILLDHDAGLDTLPGEDEKRGTVGAVALDCQGNLAAGTSTGGLTNKHPGRVGDSSIIGAGTYADNRSVAV
STTGTGEMFIRTAAAFNTAAQVRFLHAPITAAADNTLEEIAAIGGDGGLIVLDADGNYAIRFNTGAMFRGTIGEDGIART
GIFPGPETPALSC

Sequences:

>Translated_333_residues
MTIHMRGSAGNDSPIRLVIHGGAGSVKSGQGPEREQAYREALAQSLIAGHAVLAAGGNSIDAVIAAITVMEDCPLFNAGK
GAVLTHDGRNELEASIMEGATRAAGAVAGVTTIRNPIRAAHAVMTKSAHVMLIGQGAEIFAAKQDLEIVDSSYFYTRHRW
NQLQKAIAKESILLDHDAGLDTLPGEDEKRGTVGAVALDCQGNLAAGTSTGGLTNKHPGRVGDSSIIGAGTYADNRSVAV
STTGTGEMFIRTAAAFNTAAQVRFLHAPITAAADNTLEEIAAIGGDGGLIVLDADGNYAIRFNTGAMFRGTIGEDGIART
GIFPGPETPALSC
>Mature_332_residues
TIHMRGSAGNDSPIRLVIHGGAGSVKSGQGPEREQAYREALAQSLIAGHAVLAAGGNSIDAVIAAITVMEDCPLFNAGKG
AVLTHDGRNELEASIMEGATRAAGAVAGVTTIRNPIRAAHAVMTKSAHVMLIGQGAEIFAAKQDLEIVDSSYFYTRHRWN
QLQKAIAKESILLDHDAGLDTLPGEDEKRGTVGAVALDCQGNLAAGTSTGGLTNKHPGRVGDSSIIGAGTYADNRSVAVS
TTGTGEMFIRTAAAFNTAAQVRFLHAPITAAADNTLEEIAAIGGDGGLIVLDADGNYAIRFNTGAMFRGTIGEDGIARTG
IFPGPETPALSC

Specific function: May be involved in glutathione, and possibly other peptide, transport, although these results could also be due to polar effects of disruption [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI145275202, Length=317, Percent_Identity=37.5394321766561, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI145275200, Length=317, Percent_Identity=37.5394321766561, Blast_Score=178, Evalue=7e-45,
Organism=Homo sapiens, GI98991769, Length=316, Percent_Identity=30.0632911392405, Blast_Score=119, Evalue=4e-27,
Organism=Homo sapiens, GI285002251, Length=279, Percent_Identity=29.7491039426523, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI285002253, Length=279, Percent_Identity=27.9569892473118, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1787050, Length=296, Percent_Identity=50.3378378378378, Blast_Score=267, Evalue=7e-73,
Organism=Caenorhabditis elegans, GI32564649, Length=207, Percent_Identity=29.4685990338164, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17569239, Length=300, Percent_Identity=26, Blast_Score=75, Evalue=3e-14,
Organism=Drosophila melanogaster, GI18921183, Length=318, Percent_Identity=35.5345911949685, Blast_Score=149, Evalue=4e-36,
Organism=Drosophila melanogaster, GI21357893, Length=281, Percent_Identity=30.2491103202847, Blast_Score=113, Evalue=2e-25,
Organism=Drosophila melanogaster, GI221330102, Length=236, Percent_Identity=32.6271186440678, Blast_Score=98, Evalue=7e-21,
Organism=Drosophila melanogaster, GI24652135, Length=236, Percent_Identity=32.6271186440678, Blast_Score=98, Evalue=9e-21,
Organism=Drosophila melanogaster, GI24655553, Length=276, Percent_Identity=28.2608695652174, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.4.19.5 [H]

Molecular weight: Translated: 34231; Mature: 34100

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTIHMRGSAGNDSPIRLVIHGGAGSVKSGQGPEREQAYREALAQSLIAGHAVLAAGGNSI
CEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCH
DAVIAAITVMEDCPLFNAGKGAVLTHDGRNELEASIMEGATRAAGAVAGVTTIRNPIRAA
HHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
HAVMTKSAHVMLIGQGAEIFAAKQDLEIVDSSYFYTRHRWNQLQKAIAKESILLDHDAGL
HHHHHCCCEEEEEECCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCC
DTLPGEDEKRGTVGAVALDCQGNLAAGTSTGGLTNKHPGRVGDSSIIGAGTYADNRSVAV
CCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEE
STTGTGEMFIRTAAAFNTAAQVRFLHAPITAAADNTLEEIAAIGGDGGLIVLDADGNYAI
EECCCCCEEEEEHHHHCCHHEEEEEECCCHHHCCHHHHHHHHHCCCCCEEEEECCCCEEE
RFNTGAMFRGTIGEDGIARTGIFPGPETPALSC
EEECCEEEECCCCCCCCEECCCCCCCCCCCCCC
>Mature Secondary Structure 
TIHMRGSAGNDSPIRLVIHGGAGSVKSGQGPEREQAYREALAQSLIAGHAVLAAGGNSI
EEEEECCCCCCCCEEEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHCCEEEEECCCCH
DAVIAAITVMEDCPLFNAGKGAVLTHDGRNELEASIMEGATRAAGAVAGVTTIRNPIRAA
HHHHHHHHHHHCCCCCCCCCCCEEEECCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
HAVMTKSAHVMLIGQGAEIFAAKQDLEIVDSSYFYTRHRWNQLQKAIAKESILLDHDAGL
HHHHHCCCEEEEEECCCEEEEECCCCHHHCCCHHHHHHHHHHHHHHHHHHHEEEECCCCC
DTLPGEDEKRGTVGAVALDCQGNLAAGTSTGGLTNKHPGRVGDSSIIGAGTYADNRSVAV
CCCCCCCCCCCCEEEEEEECCCCEECCCCCCCCCCCCCCCCCCCCEEECCCCCCCCEEEE
STTGTGEMFIRTAAAFNTAAQVRFLHAPITAAADNTLEEIAAIGGDGGLIVLDADGNYAI
EECCCCCEEEEEHHHHCCHHEEEEEECCCHHHCCHHHHHHHHHCCCCCEEEEECCCCEEE
RFNTGAMFRGTIGEDGIARTGIFPGPETPALSC
EEECCEEEECCCCCCCCEECCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503; 3045084; 7984428 [H]