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The map label for this gene is dut

Identifier: 82703255

GI number: 82703255

Start: 2429947

End: 2430396

Strand: Reverse

Name: dut

Synonym: Nmul_A2136

Alternate gene names: 82703255

Gene position: 2430396-2429947 (Counterclockwise)

Preceding gene: 82703256

Following gene: 82703254

Centisome position: 76.33

GC content: 57.33

Gene sequence:

>450_bases
ATGAAAAAAATAGATATAAAAATTCTCGATTCGCGCCTGAAAGATCAGTTGCCCGCCTATGCGACCTCCGGCTCCGCCGG
CCTCGACTTGCGCGCGTGCATCGAGCATGTGATGACGATTCAGCCTGGAGAAGCCCATCTTATCCCCACCGGAATTGCAA
TCCATCTGTCCGACCCCGGGCTCGCCGCACTGGTGCTCCCCCGCTCCGGCCTCGGCCACAAGCACGGGATCGTGATGGGA
AATCTGGTGGGGTTGATCGACTCGGATTACCAGGGGCAGATATTCGTATCGTGCTGGAATCGGGGGCAAGCACCTTTCCT
GTTGAACCCGCTGGAGCGCATTGCGCAACTCGTAGTGGTACCCGTCGTACAGGTTGGTTTTAAAGTGGTGGATGACTTCG
AGCAAAGCGAGCGGGGCGCGAATGGGTTTGGCAGTACCGGGAAGCACTGA

Upstream 100 bases:

>100_bases
CCGGCAGGCATTCCTTGCCAAAATCGTCGAAAATCGAACAGGCGCGGCACCTCATCGCTCATGCAGCATCGCTTTATGAA
AAATGGCGGAATTTTAATTC

Downstream 100 bases:

>100_bases
TGCGCATCTTCGCCTTTATTCTCGTTTTTCTTTTATCGGCGCCAGGCAGGGCAGAAATGCCGGTACGTCAGCTCAGCATA
TCCCGTCACACTTTTTCGGT

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKKIDIKILDSRLKDQLPAYATSGSAGLDLRACIEHVMTIQPGEAHLIPTGIAIHLSDPGLAALVLPRSGLGHKHGIVMG
NLVGLIDSDYQGQIFVSCWNRGQAPFLLNPLERIAQLVVVPVVQVGFKVVDDFEQSERGANGFGSTGKH

Sequences:

>Translated_149_residues
MKKIDIKILDSRLKDQLPAYATSGSAGLDLRACIEHVMTIQPGEAHLIPTGIAIHLSDPGLAALVLPRSGLGHKHGIVMG
NLVGLIDSDYQGQIFVSCWNRGQAPFLLNPLERIAQLVVVPVVQVGFKVVDDFEQSERGANGFGSTGKH
>Mature_149_residues
MKKIDIKILDSRLKDQLPAYATSGSAGLDLRACIEHVMTIQPGEAHLIPTGIAIHLSDPGLAALVLPRSGLGHKHGIVMG
NLVGLIDSDYQGQIFVSCWNRGQAPFLLNPLERIAQLVVVPVVQVGFKVVDDFEQSERGANGFGSTGKH

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=132, Percent_Identity=37.8787878787879, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI4503423, Length=132, Percent_Identity=37.8787878787879, Blast_Score=79, Evalue=1e-15,
Organism=Homo sapiens, GI70906441, Length=132, Percent_Identity=37.8787878787879, Blast_Score=77, Evalue=6e-15,
Organism=Escherichia coli, GI1790071, Length=151, Percent_Identity=62.9139072847682, Blast_Score=206, Evalue=6e-55,
Organism=Caenorhabditis elegans, GI71988561, Length=147, Percent_Identity=39.4557823129252, Blast_Score=86, Evalue=5e-18,
Organism=Saccharomyces cerevisiae, GI6319729, Length=152, Percent_Identity=33.5526315789474, Blast_Score=73, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921126, Length=130, Percent_Identity=33.8461538461538, Blast_Score=67, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24583610, Length=130, Percent_Identity=33.8461538461538, Blast_Score=67, Evalue=4e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_NITMU (Q2Y742)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412821.1
- ProteinModelPortal:   Q2Y742
- SMR:   Q2Y742
- STRING:   Q2Y742
- GeneID:   3784762
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2136
- eggNOG:   COG0756
- HOGENOM:   HBG436079
- OMA:   LDLRACI
- PhylomeDB:   Q2Y742
- ProtClustDB:   PRK00601
- BioCyc:   NMUL323848:NMUL_A2136-MONOMER
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15974; Mature: 15974

Theoretical pI: Translated: 7.14; Mature: 7.14

Prosite motif: NA

Important sites: BINDING 81-81

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIDIKILDSRLKDQLPAYATSGSAGLDLRACIEHVMTIQPGEAHLIPTGIAIHLSDPG
CCEEEHEEHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCCCCEEEECCEEEEECCCC
LAALVLPRSGLGHKHGIVMGNLVGLIDSDYQGQIFVSCWNRGQAPFLLNPLERIAQLVVV
EEEEEECCCCCCCCCCEEEHHEEEEECCCCCCEEEEEEECCCCCCEEECHHHHHHHHHHH
PVVQVGFKVVDDFEQSERGANGFGSTGKH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MKKIDIKILDSRLKDQLPAYATSGSAGLDLRACIEHVMTIQPGEAHLIPTGIAIHLSDPG
CCEEEHEEHHHHHHHHCCCEECCCCCCCCHHHHHHHHHCCCCCCCEEEECCEEEEECCCC
LAALVLPRSGLGHKHGIVMGNLVGLIDSDYQGQIFVSCWNRGQAPFLLNPLERIAQLVVV
EEEEEECCCCCCCCCCEEEHHEEEEECCCCCCEEEEEEECCCCCCEEECHHHHHHHHHHH
PVVQVGFKVVDDFEQSERGANGFGSTGKH
HHHHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA