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The map label for this gene is lpdG [H]

Identifier: 82703195

GI number: 82703195

Start: 2366439

End: 2367941

Strand: Direct

Name: lpdG [H]

Synonym: Nmul_A2075

Alternate gene names: 82703195

Gene position: 2366439-2367941 (Clockwise)

Preceding gene: 82703194

Following gene: 82703196

Centisome position: 74.32

GC content: 57.62

Gene sequence:

>1503_bases
ATGATTCTCGCAGTTCAATTGGCAGCTCAAGGCGGAAGCACTGCATTCCATACCAATTCGTGCAACATTTCTGATGAAGA
GGAAATCCTCGTGAGTGAAACTTTCGACGTTATCATCATCGGCGCCGGCACCGCAGGATTGGCCGCTCTCAGGGAAGTAA
AGAAGCGCACTGACAATTTCGCCATTATCAACGATGGTCCCTGGGGGACAGTCTGTGCCCGTGTCGGGTGCATGCCCTCC
AAGGCACTGATCGAGGCCGCAAAGGCATTCCATCGCCGCACCTCCTTTGAGGAATTCGGCATCCAGGGCGCGCATTGCCT
CACACCGGATATTGCAGCTGTCCTGCGACGTGTGCGCCGGCTGCGGGACGATTTTGTCGCCAGCACGCTGAAGGCGACGG
AAGTGCTGGGAGAACGAGCTATTTCCGGTCGTGCGCGCCTTCTTGAAGCAGGAAGGCTCGAGGTAAACGGGCGGGAATTG
CGCGCTCGAAACATTATCATTGCTACTGGCTCCCGACCGGTTGTACCTTCTCCCTGGCTTGCACTCGACACGCAGATCCT
GACGACGGATACATTATTCGAACAAAAAACCCTTCCCGACCGGGTAGCCATCATAGGCCTGGGAGCAGTGGGCGTGGAAA
TGGCTCAAGCACTCGGCCGGCTGGGAATCGAGGTAACGGCATTCAGTGAAAGCGATACTATCGCCGGTCTGAGCGATCCG
AAAGTCAATGCGGTTGCGGCAGAGCTTTTTGCAGACGAGTTCAGGGTACATCTGGGCAAGCGGGCAGATCTCCGGGTAGC
CGATGGAGGTATGCGGGTAACTACCGGGACGCAGGAAACCGCAGTCGATGCAGTTCTTGCCAGTCTGGGCCGCAGGCCCA
ATATCGAGAATCTGGGGCTGGAAACGCTAGGCATCCCGTTGAATGAAAGAGGGCTGCCTCCTGTCGATCCCCATACCATG
CAGATAGCAGACCTGCCGGTTTTCATGGCAGGCGATGCGAGCGGCATGAGACCTCTGTTGCACGAGGCAGCGGACGAGGG
GCACATTGCAGGAATCAATGCCACCCATCCCACTCCTATTCGCTTTGATCGTCGTACGCCGCTTGCGATCGTGTTTACCG
ATCCCGGCATAGCCATCGTCGGTAAAAAACATACCTCACTTCCCCGCGGAACATTGACCGGAGAGGTTCGTTTCGAGCCC
CAGAGCCGGGCCCGCATGGCGCAACGTAACGACGGCATTCTGCGCATTTACGCCGAACCGGGCAGCGGCAAGCTGCTTGG
AGCTGAAATGTGCAGTCCCGATGCGGAGCATATGGCACACCTGCTGGCCCTGGCGATCGACCGCTCACTCACCGTGCATG
ACATGCTGCGCATGCCCTTTTATCATCCCGTCCTGGAAGAAGGTCTGCGGACTGCATTACGTGAGCTCTCGAAACAACTT
CCCCACTGTCGCGAATCCGATCTTGCAGGCTGCGAGGCGTTCGGTGCGCAGGCACTGGATTGA

Upstream 100 bases:

>100_bases
CCCTCGACGTAAATAATAACTGCCAAACAACGCACTGCTGAATAACACGTCCTTCCCTTCATCAGAACAACTGGCTGCCA
GGGGAGCTATCCTGCGATAA

Downstream 100 bases:

>100_bases
TGCAACACAGACGTCGTGCCGCAGATCTCGATATTTCATCAGCACATTGGCACATTGCCGGAACTCGGGCATAAAGTTCG
AGGATACCAAAAGCGCATAA

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: E3 component of 2-oxoglutarate dehydrogenase complex; Glycine oxidation system L-factor; LPD-GLC [H]

Number of amino acids: Translated: 500; Mature: 500

Protein sequence:

>500_residues
MILAVQLAAQGGSTAFHTNSCNISDEEEILVSETFDVIIIGAGTAGLAALREVKKRTDNFAIINDGPWGTVCARVGCMPS
KALIEAAKAFHRRTSFEEFGIQGAHCLTPDIAAVLRRVRRLRDDFVASTLKATEVLGERAISGRARLLEAGRLEVNGREL
RARNIIIATGSRPVVPSPWLALDTQILTTDTLFEQKTLPDRVAIIGLGAVGVEMAQALGRLGIEVTAFSESDTIAGLSDP
KVNAVAAELFADEFRVHLGKRADLRVADGGMRVTTGTQETAVDAVLASLGRRPNIENLGLETLGIPLNERGLPPVDPHTM
QIADLPVFMAGDASGMRPLLHEAADEGHIAGINATHPTPIRFDRRTPLAIVFTDPGIAIVGKKHTSLPRGTLTGEVRFEP
QSRARMAQRNDGILRIYAEPGSGKLLGAEMCSPDAEHMAHLLALAIDRSLTVHDMLRMPFYHPVLEEGLRTALRELSKQL
PHCRESDLAGCEAFGAQALD

Sequences:

>Translated_500_residues
MILAVQLAAQGGSTAFHTNSCNISDEEEILVSETFDVIIIGAGTAGLAALREVKKRTDNFAIINDGPWGTVCARVGCMPS
KALIEAAKAFHRRTSFEEFGIQGAHCLTPDIAAVLRRVRRLRDDFVASTLKATEVLGERAISGRARLLEAGRLEVNGREL
RARNIIIATGSRPVVPSPWLALDTQILTTDTLFEQKTLPDRVAIIGLGAVGVEMAQALGRLGIEVTAFSESDTIAGLSDP
KVNAVAAELFADEFRVHLGKRADLRVADGGMRVTTGTQETAVDAVLASLGRRPNIENLGLETLGIPLNERGLPPVDPHTM
QIADLPVFMAGDASGMRPLLHEAADEGHIAGINATHPTPIRFDRRTPLAIVFTDPGIAIVGKKHTSLPRGTLTGEVRFEP
QSRARMAQRNDGILRIYAEPGSGKLLGAEMCSPDAEHMAHLLALAIDRSLTVHDMLRMPFYHPVLEEGLRTALRELSKQL
PHCRESDLAGCEAFGAQALD
>Mature_500_residues
MILAVQLAAQGGSTAFHTNSCNISDEEEILVSETFDVIIIGAGTAGLAALREVKKRTDNFAIINDGPWGTVCARVGCMPS
KALIEAAKAFHRRTSFEEFGIQGAHCLTPDIAAVLRRVRRLRDDFVASTLKATEVLGERAISGRARLLEAGRLEVNGREL
RARNIIIATGSRPVVPSPWLALDTQILTTDTLFEQKTLPDRVAIIGLGAVGVEMAQALGRLGIEVTAFSESDTIAGLSDP
KVNAVAAELFADEFRVHLGKRADLRVADGGMRVTTGTQETAVDAVLASLGRRPNIENLGLETLGIPLNERGLPPVDPHTM
QIADLPVFMAGDASGMRPLLHEAADEGHIAGINATHPTPIRFDRRTPLAIVFTDPGIAIVGKKHTSLPRGTLTGEVRFEP
QSRARMAQRNDGILRIYAEPGSGKLLGAEMCSPDAEHMAHLLALAIDRSLTVHDMLRMPFYHPVLEEGLRTALRELSKQL
PHCRESDLAGCEAFGAQALD

Specific function: Also acts in the glycine cleavage system [H]

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=460, Percent_Identity=28.0434782608696, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI50301238, Length=472, Percent_Identity=23.3050847457627, Blast_Score=92, Evalue=9e-19,
Organism=Escherichia coli, GI1786307, Length=451, Percent_Identity=25.9423503325942, Blast_Score=125, Evalue=7e-30,
Organism=Escherichia coli, GI1789915, Length=380, Percent_Identity=27.3684210526316, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI87082354, Length=464, Percent_Identity=24.5689655172414, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI87081717, Length=461, Percent_Identity=23.8611713665944, Blast_Score=87, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI32565766, Length=461, Percent_Identity=28.1995661605206, Blast_Score=151, Evalue=7e-37,
Organism=Caenorhabditis elegans, GI71983419, Length=477, Percent_Identity=24.5283018867925, Blast_Score=92, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI71983429, Length=462, Percent_Identity=24.6753246753247, Blast_Score=91, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557007, Length=490, Percent_Identity=23.8775510204082, Blast_Score=85, Evalue=9e-17,
Organism=Saccharomyces cerevisiae, GI6321091, Length=497, Percent_Identity=26.3581488933602, Blast_Score=137, Evalue=3e-33,
Organism=Saccharomyces cerevisiae, GI6325240, Length=486, Percent_Identity=24.0740740740741, Blast_Score=101, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325166, Length=468, Percent_Identity=22.008547008547, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21358499, Length=465, Percent_Identity=28.1720430107527, Blast_Score=171, Evalue=1e-42,
Organism=Drosophila melanogaster, GI24640553, Length=520, Percent_Identity=23.4615384615385, Blast_Score=90, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24640551, Length=489, Percent_Identity=24.1308793456033, Blast_Score=89, Evalue=8e-18,
Organism=Drosophila melanogaster, GI24640549, Length=489, Percent_Identity=24.1308793456033, Blast_Score=89, Evalue=9e-18,
Organism=Drosophila melanogaster, GI17737741, Length=498, Percent_Identity=24.8995983935743, Blast_Score=87, Evalue=3e-17,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 53794; Mature: 53794

Theoretical pI: Translated: 6.28; Mature: 6.28

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MILAVQLAAQGGSTAFHTNSCNISDEEEILVSETFDVIIIGAGTAGLAALREVKKRTDNF
CEEEEEEECCCCCCEEECCCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCE
AIINDGPWGTVCARVGCMPSKALIEAAKAFHRRTSFEEFGIQGAHCLTPDIAAVLRRVRR
EEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHH
LRDDFVASTLKATEVLGERAISGRARLLEAGRLEVNGRELRARNIIIATGSRPVVPSPWL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEECCCEEEEEEEEEECCCCCCCCCCCC
ALDTQILTTDTLFEQKTLPDRVAIIGLGAVGVEMAQALGRLGIEVTAFSESDTIAGLSDP
EECCEEEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
KVNAVAAELFADEFRVHLGKRADLRVADGGMRVTTGTQETAVDAVLASLGRRPNIENLGL
CHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCC
ETLGIPLNERGLPPVDPHTMQIADLPVFMAGDASGMRPLLHEAADEGHIAGINATHPTPI
CEECCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCE
RFDRRTPLAIVFTDPGIAIVGKKHTSLPRGTLTGEVRFEPQSRARMAQRNDGILRIYAEP
EECCCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEECC
GSGKLLGAEMCSPDAEHMAHLLALAIDRSLTVHDMLRMPFYHPVLEEGLRTALRELSKQL
CCCCEEEHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PHCRESDLAGCEAFGAQALD
CCCCCCCCCCHHHCCCCCCC
>Mature Secondary Structure
MILAVQLAAQGGSTAFHTNSCNISDEEEILVSETFDVIIIGAGTAGLAALREVKKRTDNF
CEEEEEEECCCCCCEEECCCCCCCCCCCEEEECCCCEEEEECCHHHHHHHHHHHHCCCCE
AIINDGPWGTVCARVGCMPSKALIEAAKAFHRRTSFEEFGIQGAHCLTPDIAAVLRRVRR
EEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHCCCCCCCCCHHHHHHHHHHHH
LRDDFVASTLKATEVLGERAISGRARLLEAGRLEVNGRELRARNIIIATGSRPVVPSPWL
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHCCCEEECCCEEEEEEEEEECCCCCCCCCCCC
ALDTQILTTDTLFEQKTLPDRVAIIGLGAVGVEMAQALGRLGIEVTAFSESDTIAGLSDP
EECCEEEEHHHHHHHHCCCCEEEEEECCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCC
KVNAVAAELFADEFRVHLGKRADLRVADGGMRVTTGTQETAVDAVLASLGRRPNIENLGL
CHHHHHHHHHHHHHHHHCCCCCCEEEECCCEEEECCCHHHHHHHHHHHHCCCCCCCCCCC
ETLGIPLNERGLPPVDPHTMQIADLPVFMAGDASGMRPLLHEAADEGHIAGINATHPTPI
CEECCCCCCCCCCCCCCCCEEEECCCEEEECCCCCCCHHHHHCCCCCCEEECCCCCCCCE
RFDRRTPLAIVFTDPGIAIVGKKHTSLPRGTLTGEVRFEPQSRARMAQRNDGILRIYAEP
EECCCCCEEEEEECCCEEEEECCCCCCCCCCEEEEEEECCHHHHHHHHCCCCEEEEEECC
GSGKLLGAEMCSPDAEHMAHLLALAIDRSLTVHDMLRMPFYHPVLEEGLRTALRELSKQL
CCCCEEEHHHCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHC
PHCRESDLAGCEAFGAQALD
CCCCCCCCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]