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The map label for this gene is pyrF

Identifier: 82703186

GI number: 82703186

Start: 2357547

End: 2358266

Strand: Reverse

Name: pyrF

Synonym: Nmul_A2066

Alternate gene names: 82703186

Gene position: 2358266-2357547 (Counterclockwise)

Preceding gene: 82703187

Following gene: 82703184

Centisome position: 74.06

GC content: 55.56

Gene sequence:

>720_bases
ATGAATGATCCTCGTATTATCGTCGCCCTGGATTTTCCGGATTCCACATCCGCACTCGATCTTGTAGCCCGTCTGGATCC
CTCGCTATGCCGGCTTAAAGTCGGCAAAGAACTTTTTACCGCTGCCGGACCGCAGTTGGTGGAAAAGATGATGGGTAAAG
GTTTCGAAATCTTCCTCGATCTGAAATTCCACGATATTCCCACTACAGTGGCAAATGCATGCAGGACAGCCGCAGCTTTG
GGAGTGTGGATGATGAACGTGCATGCGCTGGGCGGAAGACGAATGCTGGCAGCAGCTCGTGAAGCTGTACCGCCGGGCTC
GGTCAGGCTCATCGCAGTGACGCTGCTGACCAGCATGGACCAGAGCGATCTCGATGAAGTGGGTCTGAAGGGTGAACCAC
AGGACGTGGTGCAGCGCCTTGCAGCGCTCACCCGGGATTGCGGGCTGGATGGGGTCGTATGTTCCCCGCTCGAAACTTCC
AGGCTGAGGAATGCGATAGGCGCTGACTTCTGTCTGGTCACGCCCGGAATACGGCCAGCCGATAGTTCCCCGGATGAACA
GCGGAGGATATCGACCCCTCGCCAGGCGATTGAGAACGGAGCGGATTATCTTGTTATTGGCAGACCTATTACCCAGGCGA
CTGAACCGGCAGTGATGCTGAGCCGGCTGAACAAGGATATCGAAGATATCAGGGATGTCAGTCGCTTCGACAATTTCTGA

Upstream 100 bases:

>100_bases
GACGGCAACTCGCCTCATACCGGAAAAAATATGACGTCCCGGACGTTCATTTGTAAATCAACCGTACCTTCCTGTGATTT
TCGTTCCCGATAAATTACAT

Downstream 100 bases:

>100_bases
GGGAGATGAAGCTGCACCCAGAAATCTGTCGAAGGGCTTGTTGATTTAAGAGAAAAATCGCTCTCTATTTCGCCCACAAG
TCGGATCGGGCATCGCGCTT

Product: orotidine 5'-phosphate decarboxylase

Products: NA

Alternate protein names: OMP decarboxylase; OMPDCase; OMPdecase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MNDPRIIVALDFPDSTSALDLVARLDPSLCRLKVGKELFTAAGPQLVEKMMGKGFEIFLDLKFHDIPTTVANACRTAAAL
GVWMMNVHALGGRRMLAAAREAVPPGSVRLIAVTLLTSMDQSDLDEVGLKGEPQDVVQRLAALTRDCGLDGVVCSPLETS
RLRNAIGADFCLVTPGIRPADSSPDEQRRISTPRQAIENGADYLVIGRPITQATEPAVMLSRLNKDIEDIRDVSRFDNF

Sequences:

>Translated_239_residues
MNDPRIIVALDFPDSTSALDLVARLDPSLCRLKVGKELFTAAGPQLVEKMMGKGFEIFLDLKFHDIPTTVANACRTAAAL
GVWMMNVHALGGRRMLAAAREAVPPGSVRLIAVTLLTSMDQSDLDEVGLKGEPQDVVQRLAALTRDCGLDGVVCSPLETS
RLRNAIGADFCLVTPGIRPADSSPDEQRRISTPRQAIENGADYLVIGRPITQATEPAVMLSRLNKDIEDIRDVSRFDNF
>Mature_239_residues
MNDPRIIVALDFPDSTSALDLVARLDPSLCRLKVGKELFTAAGPQLVEKMMGKGFEIFLDLKFHDIPTTVANACRTAAAL
GVWMMNVHALGGRRMLAAAREAVPPGSVRLIAVTLLTSMDQSDLDEVGLKGEPQDVVQRLAALTRDCGLDGVVCSPLETS
RLRNAIGADFCLVTPGIRPADSSPDEQRRISTPRQAIENGADYLVIGRPITQATEPAVMLSRLNKDIEDIRDVSRFDNF

Specific function: Catalyzes the decarboxylation of orotidine 5'- monophosphate (OMP) to uridine 5'-monophosphate (UMP)

COG id: COG0284

COG function: function code F; Orotidine-5'-phosphate decarboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the OMP decarboxylase family. Type 1 subfamily

Homologues:

Organism=Escherichia coli, GI1787537, Length=230, Percent_Identity=55.2173913043478, Blast_Score=245, Evalue=2e-66,

Paralogues:

None

Copy number: 6,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): PYRF_NITMU (Q2Y7B1)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412752.1
- ProteinModelPortal:   Q2Y7B1
- SMR:   Q2Y7B1
- STRING:   Q2Y7B1
- GeneID:   3784384
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A2066
- eggNOG:   COG0284
- HOGENOM:   HBG625253
- OMA:   TVHAYPQ
- PhylomeDB:   Q2Y7B1
- ProtClustDB:   PRK00230
- BioCyc:   NMUL323848:NMUL_A2066-MONOMER
- HAMAP:   MF_01200_B
- InterPro:   IPR013785
- InterPro:   IPR014732
- InterPro:   IPR018089
- InterPro:   IPR001754
- InterPro:   IPR011060
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00934
- TIGRFAMs:   TIGR01740

Pfam domain/function: PF00215 OMPdecase; SSF51366 RibP_bind_barrel

EC number: =4.1.1.23

Molecular weight: Translated: 25941; Mature: 25941

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00156 OMPDECASE

Important sites: ACT_SITE 62-62 BINDING 11-11 BINDING 33-33 BINDING 117-117 BINDING 178-178 BINDING 187-187 BINDING 207-207 BINDING 208-208

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDPRIIVALDFPDSTSALDLVARLDPSLCRLKVGKELFTAAGPQLVEKMMGKGFEIFLD
CCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEE
LKFHDIPTTVANACRTAAALGVWMMNVHALGGRRMLAAAREAVPPGSVRLIAVTLLTSMD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCC
QSDLDEVGLKGEPQDVVQRLAALTRDCGLDGVVCSPLETSRLRNAIGADFCLVTPGIRPA
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCEEEECCCCCCC
DSSPDEQRRISTPRQAIENGADYLVIGRPITQATEPAVMLSRLNKDIEDIRDVSRFDNF
CCCCHHHHHHCCHHHHHHCCCCEEEECCCCCHHCCHHHHHHHHCCHHHHHHHHHHHCCC
>Mature Secondary Structure
MNDPRIIVALDFPDSTSALDLVARLDPSLCRLKVGKELFTAAGPQLVEKMMGKGFEIFLD
CCCCEEEEEEECCCCCHHHHHHHHCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCEEEEEE
LKFHDIPTTVANACRTAAALGVWMMNVHALGGRRMLAAAREAVPPGSVRLIAVTLLTSMD
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHCC
QSDLDEVGLKGEPQDVVQRLAALTRDCGLDGVVCSPLETSRLRNAIGADFCLVTPGIRPA
HHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCEEEECCCCCCC
DSSPDEQRRISTPRQAIENGADYLVIGRPITQATEPAVMLSRLNKDIEDIRDVSRFDNF
CCCCHHHHHHCCHHHHHHCCCCEEEECCCCCHHCCHHHHHHHHCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA