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The map label for this gene is rimO

Identifier: 82703100

GI number: 82703100

Start: 2275847

End: 2277193

Strand: Direct

Name: rimO

Synonym: Nmul_A1979

Alternate gene names: 82703100

Gene position: 2275847-2277193 (Clockwise)

Preceding gene: 82703099

Following gene: 82703101

Centisome position: 71.47

GC content: 55.01

Gene sequence:

>1347_bases
ATGGCATCTCTGAAACAGCAGACTCCCCGTATCGGCTTTGTCTCCCTCGGTTGCCCGAAGGCCTTGGTAGATTCCGAGCA
GATCCTTACCCAGCTTCGTGCCGAAGGTTATGAAACTTCCTCTACCTATGAGGATGCGGACCTTGTAGTCGTCAATACCT
GCGGTTTTATCGATAGCGCAGTGGAAGAGTCACTCGACGCTATCGGCGAGGCGCTGGCGGAAAACGGCAAGGTGATCGTC
ACCGGTTGCCTTGGAGCAAAAGAAGGCGGGGACGTGGTCAAGCAAGCCCATCCACAAGTACTGGCGGTGACAGGACCCCA
CGCCCTGCCCGAAGTAATGGCTGCGGTCCACATGCATCTGCCCCAGCCGCACGATCCCTATACCAGTCTCATTCCTCCAC
AAGGCATAAAGCTGACGCCCCGGCATTACGCTTATCTCAAGATCTCCGAAGGTTGCAATCACCGCTGTACCTTCTGCATC
ATCCCTTCGATGCGCGGCGACCTCGTCAGTCGCCCCATCCATCAGGTGATGGAGGAAGCGGAAAACCTGGTGAACGCAGG
GGTCAGGGAATTGCTCGTCATTTCGCAGGATACGAGCGCTTATGGGGTAGACGTCAAATACCGCACCGGTTTCTGGCAAG
GCAGGCCGCTGAAAACGCGGATGACCGACTTGGCGCGCTCGCTCGGCGAATTGGGAGTATGGGTTCGCCTCCATTATGTT
TACCCTTACCCGCATGTCGATGAAGTGATTCCGCTCATGGCCGAGGGGAAAATTCTTCCATATCTCGATGTACCGTTTCA
GCACGCTAGTCCCCGTATCTTGAAGGCAATGAAACGTCCGGCCAACTCCGAAAACAATCTTTCCCGCATTCGGCGATGGC
GTGAAGTCTGTCCGGATATCACCCTGCGCAGTACCTTCATTGTCGGCTTTCCCGGAGAAACAGAGGCGGAATTCGAACAA
CTCCTGGAGTTTCTCGAGGAAGCGCAACTCGATCGTGTTGGCTGCTTTGCCTATTCACCTGTCGAGGGTGCGGCGGCGAA
CGCTCTTCCCGATCCCGTGCCGGAAGAGGTGAAAGAAGAGCGACGGGCGTGTTTCATGGCAATACAGGAAAAAATCAGTG
CTGAACGCCTGGCCCGCAAAATCGGCAAACGCATGATTGTCCTGATAGACGACGTAAGCAAAAACAAGGCTGTCGCCCGT
AGTACTGCCGACGCTCCGGAAATCGATGGCCTGGTTTATATCGGCAAGGCAAAAAACGTAAAACCGGGTGAATTTATTGA
AGTTGAAATTATCCGCTCCGACCCCCACGATCTGCACGCTCGACAGGTCAGCGACAACCGAACGTAA

Upstream 100 bases:

>100_bases
CGCCTTCCGGGGAATCCTCAGCCGGATATTTTGTCTCTGCAAAAACACATCATTCTTCTCACTCTGACTTTCCTTCGAGG
AAAGCGATATTACAAATCAA

Downstream 100 bases:

>100_bases
GGGATCCTGAGTAGCTATCGGAGCTATCGGTTGACATGCTCAGTATTAGGGCGATAATCCCGGTAAAAGCTGATTGCAGG
AGTCATGACGCGCTAATAAG

Product: hypothetical protein

Products: NA

Alternate protein names: S12 MTTase; S12 methylthiotransferase; Ribosome maturation factor RimO

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MASLKQQTPRIGFVSLGCPKALVDSEQILTQLRAEGYETSSTYEDADLVVVNTCGFIDSAVEESLDAIGEALAENGKVIV
TGCLGAKEGGDVVKQAHPQVLAVTGPHALPEVMAAVHMHLPQPHDPYTSLIPPQGIKLTPRHYAYLKISEGCNHRCTFCI
IPSMRGDLVSRPIHQVMEEAENLVNAGVRELLVISQDTSAYGVDVKYRTGFWQGRPLKTRMTDLARSLGELGVWVRLHYV
YPYPHVDEVIPLMAEGKILPYLDVPFQHASPRILKAMKRPANSENNLSRIRRWREVCPDITLRSTFIVGFPGETEAEFEQ
LLEFLEEAQLDRVGCFAYSPVEGAAANALPDPVPEEVKEERRACFMAIQEKISAERLARKIGKRMIVLIDDVSKNKAVAR
STADAPEIDGLVYIGKAKNVKPGEFIEVEIIRSDPHDLHARQVSDNRT

Sequences:

>Translated_448_residues
MASLKQQTPRIGFVSLGCPKALVDSEQILTQLRAEGYETSSTYEDADLVVVNTCGFIDSAVEESLDAIGEALAENGKVIV
TGCLGAKEGGDVVKQAHPQVLAVTGPHALPEVMAAVHMHLPQPHDPYTSLIPPQGIKLTPRHYAYLKISEGCNHRCTFCI
IPSMRGDLVSRPIHQVMEEAENLVNAGVRELLVISQDTSAYGVDVKYRTGFWQGRPLKTRMTDLARSLGELGVWVRLHYV
YPYPHVDEVIPLMAEGKILPYLDVPFQHASPRILKAMKRPANSENNLSRIRRWREVCPDITLRSTFIVGFPGETEAEFEQ
LLEFLEEAQLDRVGCFAYSPVEGAAANALPDPVPEEVKEERRACFMAIQEKISAERLARKIGKRMIVLIDDVSKNKAVAR
STADAPEIDGLVYIGKAKNVKPGEFIEVEIIRSDPHDLHARQVSDNRT
>Mature_447_residues
ASLKQQTPRIGFVSLGCPKALVDSEQILTQLRAEGYETSSTYEDADLVVVNTCGFIDSAVEESLDAIGEALAENGKVIVT
GCLGAKEGGDVVKQAHPQVLAVTGPHALPEVMAAVHMHLPQPHDPYTSLIPPQGIKLTPRHYAYLKISEGCNHRCTFCII
PSMRGDLVSRPIHQVMEEAENLVNAGVRELLVISQDTSAYGVDVKYRTGFWQGRPLKTRMTDLARSLGELGVWVRLHYVY
PYPHVDEVIPLMAEGKILPYLDVPFQHASPRILKAMKRPANSENNLSRIRRWREVCPDITLRSTFIVGFPGETEAEFEQL
LEFLEEAQLDRVGCFAYSPVEGAAANALPDPVPEEVKEERRACFMAIQEKISAERLARKIGKRMIVLIDDVSKNKAVARS
TADAPEIDGLVYIGKAKNVKPGEFIEVEIIRSDPHDLHARQVSDNRT

Specific function: Catalyzes the methylthiolation of an aspartic acid residue of ribosomal protein S12

COG id: COG0621

COG function: function code J; 2-methylthioadenine synthetase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRAM domain

Homologues:

Organism=Homo sapiens, GI28872784, Length=484, Percent_Identity=24.5867768595041, Blast_Score=139, Evalue=4e-33,
Organism=Homo sapiens, GI93277076, Length=453, Percent_Identity=26.4900662251656, Blast_Score=139, Evalue=5e-33,
Organism=Homo sapiens, GI28872782, Length=484, Percent_Identity=24.5867768595041, Blast_Score=139, Evalue=7e-33,
Organism=Escherichia coli, GI1787057, Length=436, Percent_Identity=68.348623853211, Blast_Score=640, Evalue=0.0,
Organism=Escherichia coli, GI1786882, Length=464, Percent_Identity=28.8793103448276, Blast_Score=168, Evalue=8e-43,
Organism=Caenorhabditis elegans, GI17553146, Length=469, Percent_Identity=24.0938166311301, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71996771, Length=292, Percent_Identity=27.3972602739726, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI21356207, Length=497, Percent_Identity=24.9496981891348, Blast_Score=136, Evalue=2e-32,
Organism=Drosophila melanogaster, GI19922432, Length=411, Percent_Identity=26.5206812652068, Blast_Score=112, Evalue=4e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RIMO_NITMU (Q2Y7J7)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412666.1
- ProteinModelPortal:   Q2Y7J7
- SMR:   Q2Y7J7
- STRING:   Q2Y7J7
- GeneID:   3785003
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1979
- eggNOG:   COG0621
- HOGENOM:   HBG457663
- OMA:   KADAPEI
- PhylomeDB:   Q2Y7J7
- BioCyc:   NMUL323848:NMUL_A1979-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01865
- InterPro:   IPR006638
- InterPro:   IPR005839
- InterPro:   IPR020612
- InterPro:   IPR013848
- InterPro:   IPR012340
- InterPro:   IPR007197
- InterPro:   IPR005840
- InterPro:   IPR002792
- Gene3D:   G3DSA:2.40.50.140
- PANTHER:   PTHR11918
- SMART:   SM00729
- TIGRFAMs:   TIGR01125
- TIGRFAMs:   TIGR00089

Pfam domain/function: PF04055 Radical_SAM; PF00919 UPF0004

EC number: NA

Molecular weight: Translated: 49651; Mature: 49519

Theoretical pI: Translated: 5.93; Mature: 5.93

Prosite motif: PS51449 MTTASE_N; PS01278 MTTASE_RADICAL; PS50926 TRAM

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASLKQQTPRIGFVSLGCPKALVDSEQILTQLRAEGYETSSTYEDADLVVVNTCGFIDSA
CCCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHH
VEESLDAIGEALAENGKVIVTGCLGAKEGGDVVKQAHPQVLAVTGPHALPEVMAAVHMHL
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHCC
PQPHDPYTSLIPPQGIKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIHQVMEEA
CCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ENLVNAGVRELLVISQDTSAYGVDVKYRTGFWQGRPLKTRMTDLARSLGELGVWVRLHYV
HHHHHHCHHHEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEE
YPYPHVDEVIPLMAEGKILPYLDVPFQHASPRILKAMKRPANSENNLSRIRRWREVCPDI
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
TLRSTFIVGFPGETEAEFEQLLEFLEEAQLDRVGCFAYSPVEGAAANALPDPVPEEVKEE
EEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RRACFMAIQEKISAERLARKIGKRMIVLIDDVSKNKAVARSTADAPEIDGLVYIGKAKNV
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCC
KPGEFIEVEIIRSDPHDLHARQVSDNRT
CCCCEEEEEEEECCCCHHHHHHCCCCCC
>Mature Secondary Structure 
ASLKQQTPRIGFVSLGCPKALVDSEQILTQLRAEGYETSSTYEDADLVVVNTCGFIDSA
CCCCCCCCCCEEEECCCCHHHCCHHHHHHHHHHCCCCCCCCCCCCCEEEEECCCHHHHH
VEESLDAIGEALAENGKVIVTGCLGAKEGGDVVKQAHPQVLAVTGPHALPEVMAAVHMHL
HHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHCCCCEEEEECCHHHHHHHHHHHHCC
PQPHDPYTSLIPPQGIKLTPRHYAYLKISEGCNHRCTFCIIPSMRGDLVSRPIHQVMEEA
CCCCCCCCCCCCCCCCEECCCEEEEEEECCCCCCCEEEEEECCCCCHHHHHHHHHHHHHH
ENLVNAGVRELLVISQDTSAYGVDVKYRTGFWQGRPLKTRMTDLARSLGELGVWVRLHYV
HHHHHHCHHHEEEEECCCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEEE
YPYPHVDEVIPLMAEGKILPYLDVPFQHASPRILKAMKRPANSENNLSRIRRWREVCPDI
CCCCCHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHCCCC
TLRSTFIVGFPGETEAEFEQLLEFLEEAQLDRVGCFAYSPVEGAAANALPDPVPEEVKEE
EEEEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCCCCCHHHHHH
RRACFMAIQEKISAERLARKIGKRMIVLIDDVSKNKAVARSTADAPEIDGLVYIGKAKNV
HHHHHHHHHHHHHHHHHHHHHCCEEEEEEECCCCCCHHHHCCCCCCCCCCEEEECCCCCC
KPGEFIEVEIIRSDPHDLHARQVSDNRT
CCCCEEEEEEEECCCCHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA