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The map label for this gene is mnmC

Identifier: 82703048

GI number: 82703048

Start: 2216478

End: 2218331

Strand: Direct

Name: mnmC

Synonym: Nmul_A1927

Alternate gene names: 82703048

Gene position: 2216478-2218331 (Clockwise)

Preceding gene: 82703047

Following gene: 82703049

Centisome position: 69.61

GC content: 55.77

Gene sequence:

>1854_bases
ATGCTTGACTGGCAAAACGGACAACTGTATTCCACTCGCTTCGGTGACGTCTACTTCTCAAGAGACTCGGGACTGGAAGA
AAAACAGTACGTCTTCCTGCAGGGCAACCGGCTTGCGGACCGTTTTGAGTCCTTGCAGCCTGATACCGCGTTTTCCATTG
GAGAAACGGGATTCGGCACAGGATTGAGCTTTTTATGCACTTGGCGGCTATTCATCCAGATTGCACCCCTTCGGACCAGT
CTTGATTTTTTCAGTGTTGAAAAATATCCGCTTGATGAAAAAGAACTGAGCGCAGCGCTCGCGCTTTGGCCCGAACTGGG
CCCATACGCCGATGAACTTATGCTGCGCTGGCAGCGGCGTGTACCCGGATGGAATCGGTGGAGCTTCGCCGGAGGAAGAG
TGCGTCTCACGCTGGCAATAGAGGACGTGACCCGGGCGCTGCCTGAAACGCACGGTATCGATGCATGGTTTCTTGACGGC
TTTTCACCGGCGCGAAACCCGGAAATGTGGACACTCCAGATTTTTCACTGGATTGCGCGGGCGTCGCGAGCAGGCGCAAC
CTTTGCGACCTATACCAGTGCCGGCGTTGTTCGTCGCGGTTTGGAACAAGCAGGGTTTCAGGTCAAAAAAATATCCGGCT
TTGGCCATAAGCGTGAAATGCTGCAAGGTGACCTTCCTGGCCCCCCTCCCGTTCGACTGGCTCCCACCACCGCGATCGTT
ATCGGAGGGGGAATAGCAGGGTGCGCCGCTGCTTCGGCGCTGGCCAGTCGTGGACTTATAGTTGAACTTCTGGAATCACA
CACCCTTGGCGCGGGTGCGTCAGGCAACCCGATTGGTATACTGCACGCCCGCCTGAGTGCAGGAATGAACGCCCTGCACC
GCTTTGTGCTGGCATCCTACGGACATGCGCTCGCCTTGCTTGACGAAAAAATACCCGTCGATGGCGTCATGCGGAGTGAA
TGCGGAGAACTGCAGCTGTCATTCTCCGCCGAAGAAGCAAGACGAATCGGGAAGCTTGCGACCCTCGACTGGCCCGCGCA
TGTTTTCCGACCAGTAGATGCGGCTGAAGCATCGGCCCTTGCGGGAATTGAGCTTTCATATGGTGGCCTTTGGTTTCCCG
GTAGCGGTTGGCTTGCTCCGCCTCAACTTTGTGTAGCCTTGCTTGGCAGTCAGGCTATCACCCTGTATACCGGTCGCACG
GTAAAATCACTTACCCCAACGAGTCACGGGTGGCGTGTGCAAGCGGAAGATCAGAGGAAGCAAGCGTGGTCTCTGGAGGC
CGAGATAGTTGTGGTTTGCACCGGATATCAGGTGAAATCGCTTCCAGCATTGGCAAATCTGCCGCTAACCCCGGTACGGG
GACAGCTTACCTTGATCCCTGCAACAACCGCAAGCCAGAATCTCCGCACCATCGTATGCGGGAGTGGCTATTTCTCCCCT
GCTGTTGCAGGACGACATATGGTGGGAGCAACCCATCGTTTTAACGATACATCGATTAACCTGAATGTATCGGAGCATGC
GGAAAACTTATCCAGACTGCGAGAAATTTCTCCTGTCCTCCGCAGGTTGAGTGACGAGGTAAGTCAAGATATCAGGCAGC
TTGAGCAATTGGATGGACGCACATCTATCAGGGGGTCTGTTCCAGGCGCCATGCCGCTCGTCGGCGAACTTTTGCCCGGA
CTGTATACCAGCCTCGGCCATGGAACGCGTGGACTGATTACCGCGGGAATTTCAGCCGAATTGGTCGCGGCAACCGCCTG
CGGGCAACTGCTGCCGTTGCCATTGTCCGTTGTCAATGCGCTCTCGCCTGTCCGAAGAGCTTCTCCCGCTATTCCGGTTT
CAATCAAGGGATAG

Upstream 100 bases:

>100_bases
CAAGAAAACAGTGGAACCAGTCAGGTTGCACCGGCACATAGACAACGTCGATTGATGATGAATCCGGCTTTTCGCCAGCA
TACCGGCTGAATCTTCCCGC

Downstream 100 bases:

>100_bases
GGTGTACTAGAGTCCAATGGGCAACGTGGTTGAAAACCAGTAGAATACGCACCCATCTCTCTCGGATAATTCACCCTTTA
CATCTTGGATGCCAGGCCGC

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA mnm(5)s(2)U biosynthesis bifunctional protein; tRNA (mnm(5)s(2)U34)-methyltransferase; FAD-dependent cmnm(5)s(2)U34 oxidoreductase

Number of amino acids: Translated: 617; Mature: 617

Protein sequence:

>617_residues
MLDWQNGQLYSTRFGDVYFSRDSGLEEKQYVFLQGNRLADRFESLQPDTAFSIGETGFGTGLSFLCTWRLFIQIAPLRTS
LDFFSVEKYPLDEKELSAALALWPELGPYADELMLRWQRRVPGWNRWSFAGGRVRLTLAIEDVTRALPETHGIDAWFLDG
FSPARNPEMWTLQIFHWIARASRAGATFATYTSAGVVRRGLEQAGFQVKKISGFGHKREMLQGDLPGPPPVRLAPTTAIV
IGGGIAGCAAASALASRGLIVELLESHTLGAGASGNPIGILHARLSAGMNALHRFVLASYGHALALLDEKIPVDGVMRSE
CGELQLSFSAEEARRIGKLATLDWPAHVFRPVDAAEASALAGIELSYGGLWFPGSGWLAPPQLCVALLGSQAITLYTGRT
VKSLTPTSHGWRVQAEDQRKQAWSLEAEIVVVCTGYQVKSLPALANLPLTPVRGQLTLIPATTASQNLRTIVCGSGYFSP
AVAGRHMVGATHRFNDTSINLNVSEHAENLSRLREISPVLRRLSDEVSQDIRQLEQLDGRTSIRGSVPGAMPLVGELLPG
LYTSLGHGTRGLITAGISAELVAATACGQLLPLPLSVVNALSPVRRASPAIPVSIKG

Sequences:

>Translated_617_residues
MLDWQNGQLYSTRFGDVYFSRDSGLEEKQYVFLQGNRLADRFESLQPDTAFSIGETGFGTGLSFLCTWRLFIQIAPLRTS
LDFFSVEKYPLDEKELSAALALWPELGPYADELMLRWQRRVPGWNRWSFAGGRVRLTLAIEDVTRALPETHGIDAWFLDG
FSPARNPEMWTLQIFHWIARASRAGATFATYTSAGVVRRGLEQAGFQVKKISGFGHKREMLQGDLPGPPPVRLAPTTAIV
IGGGIAGCAAASALASRGLIVELLESHTLGAGASGNPIGILHARLSAGMNALHRFVLASYGHALALLDEKIPVDGVMRSE
CGELQLSFSAEEARRIGKLATLDWPAHVFRPVDAAEASALAGIELSYGGLWFPGSGWLAPPQLCVALLGSQAITLYTGRT
VKSLTPTSHGWRVQAEDQRKQAWSLEAEIVVVCTGYQVKSLPALANLPLTPVRGQLTLIPATTASQNLRTIVCGSGYFSP
AVAGRHMVGATHRFNDTSINLNVSEHAENLSRLREISPVLRRLSDEVSQDIRQLEQLDGRTSIRGSVPGAMPLVGELLPG
LYTSLGHGTRGLITAGISAELVAATACGQLLPLPLSVVNALSPVRRASPAIPVSIKG
>Mature_617_residues
MLDWQNGQLYSTRFGDVYFSRDSGLEEKQYVFLQGNRLADRFESLQPDTAFSIGETGFGTGLSFLCTWRLFIQIAPLRTS
LDFFSVEKYPLDEKELSAALALWPELGPYADELMLRWQRRVPGWNRWSFAGGRVRLTLAIEDVTRALPETHGIDAWFLDG
FSPARNPEMWTLQIFHWIARASRAGATFATYTSAGVVRRGLEQAGFQVKKISGFGHKREMLQGDLPGPPPVRLAPTTAIV
IGGGIAGCAAASALASRGLIVELLESHTLGAGASGNPIGILHARLSAGMNALHRFVLASYGHALALLDEKIPVDGVMRSE
CGELQLSFSAEEARRIGKLATLDWPAHVFRPVDAAEASALAGIELSYGGLWFPGSGWLAPPQLCVALLGSQAITLYTGRT
VKSLTPTSHGWRVQAEDQRKQAWSLEAEIVVVCTGYQVKSLPALANLPLTPVRGQLTLIPATTASQNLRTIVCGSGYFSP
AVAGRHMVGATHRFNDTSINLNVSEHAENLSRLREISPVLRRLSDEVSQDIRQLEQLDGRTSIRGSVPGAMPLVGELLPG
LYTSLGHGTRGLITAGISAELVAATACGQLLPLPLSVVNALSPVRRASPAIPVSIKG

Specific function: Catalyzes the last two steps in the biosynthesis of 5- methylaminomethyl-2-thiouridine (mnm(5)s(2)U) at the wobble position (U34) in tRNA. Catalyzes the FAD-dependent demodification of cmnm(5)s(2)U34 to nm(5)s(2)U34, followed by the transfer of a methyl g

COG id: COG4121

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the DAO family

Homologues:

Organism=Escherichia coli, GI87082084, Length=647, Percent_Identity=33.2302936630603, Blast_Score=313, Evalue=2e-86,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNMC_NITMU (Q2Y7P9)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412614.1
- ProteinModelPortal:   Q2Y7P9
- SMR:   Q2Y7P9
- STRING:   Q2Y7P9
- GeneID:   3784223
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1927
- eggNOG:   COG0665
- HOGENOM:   HBG677640
- OMA:   VIGETGF
- PhylomeDB:   Q2Y7P9
- ProtClustDB:   CLSK2772839
- BioCyc:   NMUL323848:NMUL_A1927-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01102
- InterPro:   IPR008471
- InterPro:   IPR006076
- InterPro:   IPR023032
- InterPro:   IPR017610
- TIGRFAMs:   TIGR03197

Pfam domain/function: PF01266 DAO; PF05430 DUF752

EC number: =2.1.1.61

Molecular weight: Translated: 66615; Mature: 66615

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDWQNGQLYSTRFGDVYFSRDSGLEEKQYVFLQGNRLADRFESLQPDTAFSIGETGFGT
CCCCCCCCEEEEECCCEEEECCCCCCCCEEEEEECCHHHHHHHHCCCCCCEECCCCCCCC
GLSFLCTWRLFIQIAPLRTSLDFFSVEKYPLDEKELSAALALWPELGPYADELMLRWQRR
CHHHHHHHHHHHEEECHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHC
VPGWNRWSFAGGRVRLTLAIEDVTRALPETHGIDAWFLDGFSPARNPEMWTLQIFHWIAR
CCCCCCEEECCCEEEEEEEEHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHH
ASRAGATFATYTSAGVVRRGLEQAGFQVKKISGFGHKREMLQGDLPGPPPVRLAPTTAIV
HHCCCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHCCCCCCCCCEEECCCEEEE
IGGGIAGCAAASALASRGLIVELLESHTLGAGASGNPIGILHARLSAGMNALHRFVLASY
ECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHC
GHALALLDEKIPVDGVMRSECGELQLSFSAEEARRIGKLATLDWPAHVFRPVDAAEASAL
CCEEEEECCCCCCCCHHHCCCCCEEEEECHHHHHHHCCEEEECCCHHHHCCCCHHHHHHH
AGIELSYGGLWFPGSGWLAPPQLCVALLGSQAITLYTGRTVKSLTPTSHGWRVQAEDQRK
CCEEEECCCEEECCCCCCCCHHHHHHHHCCCEEEEEECCEEECCCCCCCCCEEECHHHHH
QAWSLEAEIVVVCTGYQVKSLPALANLPLTPVRGQLTLIPATTASQNLRTIVCGSGYFSP
HHHCCCEEEEEEECCCEEECCCHHHCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCH
AVAGRHMVGATHRFNDTSINLNVSEHAENLSRLREISPVLRRLSDEVSQDIRQLEQLDGR
HHCCCHHCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSIRGSVPGAMPLVGELLPGLYTSLGHGTRGLITAGISAELVAATACGQLLPLPLSVVNA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHH
LSPVRRASPAIPVSIKG
HHHHHHCCCCCEEEECC
>Mature Secondary Structure
MLDWQNGQLYSTRFGDVYFSRDSGLEEKQYVFLQGNRLADRFESLQPDTAFSIGETGFGT
CCCCCCCCEEEEECCCEEEECCCCCCCCEEEEEECCHHHHHHHHCCCCCCEECCCCCCCC
GLSFLCTWRLFIQIAPLRTSLDFFSVEKYPLDEKELSAALALWPELGPYADELMLRWQRR
CHHHHHHHHHHHEEECHHHCCCCEECCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHC
VPGWNRWSFAGGRVRLTLAIEDVTRALPETHGIDAWFLDGFSPARNPEMWTLQIFHWIAR
CCCCCCEEECCCEEEEEEEEHHHHHHCCCCCCCCEEEECCCCCCCCCCEEEHHHHHHHHH
ASRAGATFATYTSAGVVRRGLEQAGFQVKKISGFGHKREMLQGDLPGPPPVRLAPTTAIV
HHCCCCEEEEHHHHHHHHHHHHHHCCEEEEECCCCHHHHHHCCCCCCCCCEEECCCEEEE
IGGGIAGCAAASALASRGLIVELLESHTLGAGASGNPIGILHARLSAGMNALHRFVLASY
ECCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHHC
GHALALLDEKIPVDGVMRSECGELQLSFSAEEARRIGKLATLDWPAHVFRPVDAAEASAL
CCEEEEECCCCCCCCHHHCCCCCEEEEECHHHHHHHCCEEEECCCHHHHCCCCHHHHHHH
AGIELSYGGLWFPGSGWLAPPQLCVALLGSQAITLYTGRTVKSLTPTSHGWRVQAEDQRK
CCEEEECCCEEECCCCCCCCHHHHHHHHCCCEEEEEECCEEECCCCCCCCCEEECHHHHH
QAWSLEAEIVVVCTGYQVKSLPALANLPLTPVRGQLTLIPATTASQNLRTIVCGSGYFSP
HHHCCCEEEEEEECCCEEECCCHHHCCCCCCCCCEEEEEECCCCCCCCEEEEECCCCCCH
AVAGRHMVGATHRFNDTSINLNVSEHAENLSRLREISPVLRRLSDEVSQDIRQLEQLDGR
HHCCCHHCCCEEECCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
TSIRGSVPGAMPLVGELLPGLYTSLGHGTRGLITAGISAELVAATACGQLLPLPLSVVNA
CCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCCCCHHHHHHH
LSPVRRASPAIPVSIKG
HHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA