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The map label for this gene is proB

Identifier: 82702964

GI number: 82702964

Start: 2127049

End: 2128167

Strand: Direct

Name: proB

Synonym: Nmul_A1843

Alternate gene names: 82702964

Gene position: 2127049-2128167 (Clockwise)

Preceding gene: 82702963

Following gene: 82702971

Centisome position: 66.8

GC content: 58.18

Gene sequence:

>1119_bases
ATGGAATCCGTTTTAAAGCAGTCGCGGCGCATTGTCGTCAAGGTCGGCAGCAGCCTGGTAACGAACCAGGGACAAGGCCT
TGACCATTCGGCGCTCGCGCACTGGGCTGGACAAATCGTTAAACTCAAGAAGACCGGCAAGGAAGTGGTACTCGTTTCTT
CCGGGGCCATAGCGGAGGGAATGCAACGCCTGAAATGGAAAAAGCGCCCCCACGCGGTCCATGAATTGCAGGCGGCAGCG
GCGGTGGGACAAATGGGCCTGGTGCAGGCCTATGAAACCTGCTTCCGCAAGCATGAATTGCACTCGGCCCAGATTTTGCT
CACCCACGAAGACTTGTCCGACCGCAAGCGCTATCTGAACGCACGCTCCACGCTGGCCACGCTGTTGGATCTGAATGTCA
TTCCAATCATCAATGAAAATGATACGGTAGCAACCGATGAAATCCGCTTCGGCGATAACGATACGCTAGCCGCACTGGTA
ACCAACCTGATCGAGGCGGATGTTCTGGTAATTCTAACCGATCAGCGAGGGTTATTCACAGCCGATCCGCGAAAAGATAC
CCAGGCGAAACTGGTGGAGCAAGCAAAAGCCGGAGACGCGGACATCGAACGGATGGCGGGGGGGGCAGGCAGCGAAATAG
GACGGGGCGGCATGTTGACCAAGGTGCTGGCGGCCAAACGCGCAGCGAGAAGCGGCGCGCACACGGTGATTGCGTCGGGA
CGCGAAGCGGATGTCTTGATTCGCCTCGCCCGAGGGGAAGCAATCGGCACCCAGTTGCTGGCGGAAACCATGACGCTTGC
CGCTCGCAAGCAGTGGCTGGCGGACCATCTGCAAATCCGGGGAAATGTCACGCTGGACGAAGGAGCGGTGGAAGCACTGG
CCGTTGGCGGCAAAAGTCTTCTGCCGGTCGGCGTGACCGATGTGCGCGGCAACTTCGAACGGGGTGAAGTCGTAGGCTGC
CTCGATCCGCACGGAAGGGAAATCGCTCGCGGACTGGTCAATTACAATGCTGCGGAAACCCGGCGTATTCTCCGCCGGGC
AAGCGACGAAATCGAAAGCCTTCTCGGCTATATTGGTGAACCGGAGCTCATCCACAGGGATAACCTGGTTCTGCTTTAA

Upstream 100 bases:

>100_bases
TCAAACTACCCTTGATTTGATTTTTTCGATATCTGGAATAGATATCCGGTATTGAAAACCTGGATACGGGCAACCGCACA
TTCATTGTAAAGCGTCAAAT

Downstream 100 bases:

>100_bases
CACCTCTATGGGAAAGCGGAACCATGAGTCCAGATAACTTCTTGAAATCTGGATACCCCCTTTTCGCGGGTATGGCACAT
AATGGAGCGATGCGGAAATC

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MESVLKQSRRIVVKVGSSLVTNQGQGLDHSALAHWAGQIVKLKKTGKEVVLVSSGAIAEGMQRLKWKKRPHAVHELQAAA
AVGQMGLVQAYETCFRKHELHSAQILLTHEDLSDRKRYLNARSTLATLLDLNVIPIINENDTVATDEIRFGDNDTLAALV
TNLIEADVLVILTDQRGLFTADPRKDTQAKLVEQAKAGDADIERMAGGAGSEIGRGGMLTKVLAAKRAARSGAHTVIASG
READVLIRLARGEAIGTQLLAETMTLAARKQWLADHLQIRGNVTLDEGAVEALAVGGKSLLPVGVTDVRGNFERGEVVGC
LDPHGREIARGLVNYNAAETRRILRRASDEIESLLGYIGEPELIHRDNLVLL

Sequences:

>Translated_372_residues
MESVLKQSRRIVVKVGSSLVTNQGQGLDHSALAHWAGQIVKLKKTGKEVVLVSSGAIAEGMQRLKWKKRPHAVHELQAAA
AVGQMGLVQAYETCFRKHELHSAQILLTHEDLSDRKRYLNARSTLATLLDLNVIPIINENDTVATDEIRFGDNDTLAALV
TNLIEADVLVILTDQRGLFTADPRKDTQAKLVEQAKAGDADIERMAGGAGSEIGRGGMLTKVLAAKRAARSGAHTVIASG
READVLIRLARGEAIGTQLLAETMTLAARKQWLADHLQIRGNVTLDEGAVEALAVGGKSLLPVGVTDVRGNFERGEVVGC
LDPHGREIARGLVNYNAAETRRILRRASDEIESLLGYIGEPELIHRDNLVLL
>Mature_372_residues
MESVLKQSRRIVVKVGSSLVTNQGQGLDHSALAHWAGQIVKLKKTGKEVVLVSSGAIAEGMQRLKWKKRPHAVHELQAAA
AVGQMGLVQAYETCFRKHELHSAQILLTHEDLSDRKRYLNARSTLATLLDLNVIPIINENDTVATDEIRFGDNDTLAALV
TNLIEADVLVILTDQRGLFTADPRKDTQAKLVEQAKAGDADIERMAGGAGSEIGRGGMLTKVLAAKRAARSGAHTVIASG
READVLIRLARGEAIGTQLLAETMTLAARKQWLADHLQIRGNVTLDEGAVEALAVGGKSLLPVGVTDVRGNFERGEVVGC
LDPHGREIARGLVNYNAAETRRILRRASDEIESLLGYIGEPELIHRDNLVLL

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI62912457, Length=288, Percent_Identity=35.7638888888889, Blast_Score=143, Evalue=3e-34,
Organism=Homo sapiens, GI21361368, Length=290, Percent_Identity=35.5172413793103, Blast_Score=142, Evalue=4e-34,
Organism=Escherichia coli, GI1786437, Length=366, Percent_Identity=42.3497267759563, Blast_Score=283, Evalue=9e-78,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=35.0553505535055, Blast_Score=153, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=35.0553505535055, Blast_Score=153, Evalue=2e-37,
Organism=Saccharomyces cerevisiae, GI6320506, Length=419, Percent_Identity=35.0835322195704, Blast_Score=213, Evalue=6e-56,
Organism=Saccharomyces cerevisiae, GI6321822, Length=423, Percent_Identity=32.1513002364066, Blast_Score=184, Evalue=3e-47,
Organism=Drosophila melanogaster, GI21357643, Length=287, Percent_Identity=33.4494773519164, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_NITMU (Q2Y7Y3)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412530.1
- ProteinModelPortal:   Q2Y7Y3
- SMR:   Q2Y7Y3
- STRING:   Q2Y7Y3
- GeneID:   3785952
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1843
- eggNOG:   COG0263
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- PhylomeDB:   Q2Y7Y3
- ProtClustDB:   PRK05429
- BioCyc:   NMUL323848:NMUL_A1843-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40228; Mature: 40228

Theoretical pI: Translated: 7.81; Mature: 7.81

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESVLKQSRRIVVKVGSSLVTNQGQGLDHSALAHWAGQIVKLKKTGKEVVLVSSGAIAEG
CCHHHHHCCEEEEEECCHHHCCCCCCCCHHHHHHHHHHEEEEECCCCEEEEEECCHHHHH
MQRLKWKKRPHAVHELQAAAAVGQMGLVQAYETCFRKHELHSAQILLTHEDLSDRKRYLN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
ARSTLATLLDLNVIPIINENDTVATDEIRFGDNDTLAALVTNLIEADVLVILTDQRGLFT
HHHHHHHHHCCCEEEEECCCCCEEECEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDTQAKLVEQAKAGDADIERMAGGAGSEIGRGGMLTKVLAAKRAARSGAHTVIASG
CCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
READVLIRLARGEAIGTQLLAETMTLAARKQWLADHLQIRGNVTLDEGAVEALAVGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEECCCHHHHHHCCCCCC
LPVGVTDVRGNFERGEVVGCLDPHGREIARGLVNYNAAETRRILRRASDEIESLLGYIGE
CCCCCHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
PELIHRDNLVLL
CCEEECCCEEEC
>Mature Secondary Structure
MESVLKQSRRIVVKVGSSLVTNQGQGLDHSALAHWAGQIVKLKKTGKEVVLVSSGAIAEG
CCHHHHHCCEEEEEECCHHHCCCCCCCCHHHHHHHHHHEEEEECCCCEEEEEECCHHHHH
MQRLKWKKRPHAVHELQAAAAVGQMGLVQAYETCFRKHELHSAQILLTHEDLSDRKRYLN
HHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEECCHHHHHHHHH
ARSTLATLLDLNVIPIINENDTVATDEIRFGDNDTLAALVTNLIEADVLVILTDQRGLFT
HHHHHHHHHCCCEEEEECCCCCEEECEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCEE
ADPRKDTQAKLVEQAKAGDADIERMAGGAGSEIGRGGMLTKVLAAKRAARSGAHTVIASG
CCCCCCHHHHHHHHHCCCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCCEEEECC
READVLIRLARGEAIGTQLLAETMTLAARKQWLADHLQIRGNVTLDEGAVEALAVGGKSL
CCCEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHEECCCEEECCCHHHHHHCCCCCC
LPVGVTDVRGNFERGEVVGCLDPHGREIARGLVNYNAAETRRILRRASDEIESLLGYIGE
CCCCCHHCCCCCCCCCEEEECCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCC
PELIHRDNLVLL
CCEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA