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The map label for this gene is gltX

Identifier: 82702731

GI number: 82702731

Start: 1838484

End: 1839881

Strand: Reverse

Name: gltX

Synonym: Nmul_A1604

Alternate gene names: 82702731

Gene position: 1839881-1838484 (Counterclockwise)

Preceding gene: 82702732

Following gene: 82702730

Centisome position: 57.78

GC content: 55.72

Gene sequence:

>1398_bases
ATGATTCGCACTCGTTTCGCTCCCAGTCCTACGGGCTATCTTCATATTGGCGGCGCTCGCACGGCGCTGTTTTCCTGGGC
TTATGCCCGCAAGCATGGCGGTAAATTCGTCCTTCGGATTGAAGATACGGATCTGGAACGTTCCACTGCTCAATCAACCC
AAGCGATTCTGGACGGTATGGCGTGGCTGGGGCTGGACTATGACGAGGGGCCGTTTTACCAGATGCAGCGGCTGGCCCGC
TACCATGAAGTAGCGGAGCAGTTGTTGCGCAGGGGGCAGGCCTATTACTGCTATTGCAGCAGGGAAGAACTGGATGCAAT
GCGGGAGCAGCAGCGCGCGGCGGGATTGAAGCCGCGTTATGATGGTCGCTGGCGTGATTCCAGGGAGGAACCTCCTGCCG
GTGTGAAGCCGGTCGTGCGATTGAAAAATCCGCTGGATGGGGAGGTAACATTCAAGGACCTGATTAAGGGTGAAATCACC
GTTGCCAACAGTGAGCTGGATGATCTCGTGCTGTTGCGCGGGGATGGAGTGCCGACTTATAACTTTGGCGTGGTGATTGA
CGATCTCGATATGAACATCACGCATGTGATTCGGGGTGATGATCACGTGAACAACACACCGCGCCAGATCAATATATTGA
AGGCCTTGGGCGCGCCTCTGCCGCAATATGCGCATGTGCCGATGATTCTCGGTGCGGATGGCGAGCGTTTATCGAAGCGG
CATGGCGCGGTCTCGGTGATGCAATATCGTGAAGATGGTTACCTGCCAGAGGCGTTGGTGAATTATCTGGCCCGGCTGGG
CTGGTCGCATGGTGATGAGGAAATATTCAGCCGGGAGCAGTTGGTGGAGTGGTTCGATTTATCCAATATCAATCGCTCAC
CGGCGAAGTTCAATCCGGAAAAATTGCAATGGCTCAACCAGCAGTACCTGAAAACGGCGGACAACGAGCGGCTGGCGGAA
TTGGTAAAACCTTTTCTCGCGGCGGACGGCTGTGACGTTACGGGCGGAGGGACCCCCGATTTGCGCAAGGTTATGAATCT
GCTCAAGGAGCGCGTCAATACCATTGCGGAACTGGCAGATGCGGCCGTTTATTTCTTCCGGCCGCTGGAACCTGCCGAGG
AACTGAGAGCACAGTATTTCTCCGCTGAGGCTAAAGGCCCAATTCTCGATTTGCGGACAAAACTTGCCACCCTCGAATGG
GAAGGCCATGCCATCAACGACGCAATCAAGGCCAGCGCTACGGCACATGGCGTGAAGATGCCGAAGGTGGCAATGCCATT
ACGCGTTATGGTTACAGGAGAAGCCCAGACTCCGGCGATCAATGCGGTGCTGGAACTGCTGGGGAGGGAGGAAACGCTGC
GGAGGATGGACAGGCAGCTTGAGTACTTTTTGAGCTGA

Upstream 100 bases:

>100_bases
TGCGGGCCGCAGGAATGTTAAGTCACGCCTGACTTACATTCTTGTAAAATGGCGGGTTTCCACAACTGTCGAGCTGATTC
CTGTAATCGGCGCCCCACCC

Downstream 100 bases:

>100_bases
TAATCAGGAATAAATGCCGGGAATATTCCACGCCCCATCCTGCCGCCGTTCCCCTTTTCGCTCTCACCTTCGACGCGCAT
GGGCCCTAAGCCTGTGGAAA

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MIRTRFAPSPTGYLHIGGARTALFSWAYARKHGGKFVLRIEDTDLERSTAQSTQAILDGMAWLGLDYDEGPFYQMQRLAR
YHEVAEQLLRRGQAYYCYCSREELDAMREQQRAAGLKPRYDGRWRDSREEPPAGVKPVVRLKNPLDGEVTFKDLIKGEIT
VANSELDDLVLLRGDGVPTYNFGVVIDDLDMNITHVIRGDDHVNNTPRQINILKALGAPLPQYAHVPMILGADGERLSKR
HGAVSVMQYREDGYLPEALVNYLARLGWSHGDEEIFSREQLVEWFDLSNINRSPAKFNPEKLQWLNQQYLKTADNERLAE
LVKPFLAADGCDVTGGGTPDLRKVMNLLKERVNTIAELADAAVYFFRPLEPAEELRAQYFSAEAKGPILDLRTKLATLEW
EGHAINDAIKASATAHGVKMPKVAMPLRVMVTGEAQTPAINAVLELLGREETLRRMDRQLEYFLS

Sequences:

>Translated_465_residues
MIRTRFAPSPTGYLHIGGARTALFSWAYARKHGGKFVLRIEDTDLERSTAQSTQAILDGMAWLGLDYDEGPFYQMQRLAR
YHEVAEQLLRRGQAYYCYCSREELDAMREQQRAAGLKPRYDGRWRDSREEPPAGVKPVVRLKNPLDGEVTFKDLIKGEIT
VANSELDDLVLLRGDGVPTYNFGVVIDDLDMNITHVIRGDDHVNNTPRQINILKALGAPLPQYAHVPMILGADGERLSKR
HGAVSVMQYREDGYLPEALVNYLARLGWSHGDEEIFSREQLVEWFDLSNINRSPAKFNPEKLQWLNQQYLKTADNERLAE
LVKPFLAADGCDVTGGGTPDLRKVMNLLKERVNTIAELADAAVYFFRPLEPAEELRAQYFSAEAKGPILDLRTKLATLEW
EGHAINDAIKASATAHGVKMPKVAMPLRVMVTGEAQTPAINAVLELLGREETLRRMDRQLEYFLS
>Mature_465_residues
MIRTRFAPSPTGYLHIGGARTALFSWAYARKHGGKFVLRIEDTDLERSTAQSTQAILDGMAWLGLDYDEGPFYQMQRLAR
YHEVAEQLLRRGQAYYCYCSREELDAMREQQRAAGLKPRYDGRWRDSREEPPAGVKPVVRLKNPLDGEVTFKDLIKGEIT
VANSELDDLVLLRGDGVPTYNFGVVIDDLDMNITHVIRGDDHVNNTPRQINILKALGAPLPQYAHVPMILGADGERLSKR
HGAVSVMQYREDGYLPEALVNYLARLGWSHGDEEIFSREQLVEWFDLSNINRSPAKFNPEKLQWLNQQYLKTADNERLAE
LVKPFLAADGCDVTGGGTPDLRKVMNLLKERVNTIAELADAAVYFFRPLEPAEELRAQYFSAEAKGPILDLRTKLATLEW
EGHAINDAIKASATAHGVKMPKVAMPLRVMVTGEAQTPAINAVLELLGREETLRRMDRQLEYFLS

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu)

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI134288884, Length=488, Percent_Identity=31.9672131147541, Blast_Score=229, Evalue=4e-60,
Organism=Homo sapiens, GI4826960, Length=299, Percent_Identity=26.4214046822742, Blast_Score=80, Evalue=3e-15,
Organism=Escherichia coli, GI1788743, Length=464, Percent_Identity=53.0172413793103, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI226510925, Length=282, Percent_Identity=33.3333333333333, Blast_Score=133, Evalue=2e-32,
Organism=Escherichia coli, GI1786895, Length=339, Percent_Identity=22.7138643067847, Blast_Score=65, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI71993394, Length=477, Percent_Identity=32.2851153039832, Blast_Score=226, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI17511007, Length=234, Percent_Identity=24.3589743589744, Blast_Score=66, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324540, Length=491, Percent_Identity=31.1608961303462, Blast_Score=242, Evalue=1e-64,
Organism=Saccharomyces cerevisiae, GI6324742, Length=223, Percent_Identity=28.2511210762332, Blast_Score=86, Evalue=1e-17,
Organism=Saccharomyces cerevisiae, GI6321192, Length=231, Percent_Identity=27.2727272727273, Blast_Score=77, Evalue=8e-15,
Organism=Drosophila melanogaster, GI24665321, Length=486, Percent_Identity=32.5102880658436, Blast_Score=256, Evalue=2e-68,
Organism=Drosophila melanogaster, GI17864488, Length=310, Percent_Identity=25.4838709677419, Blast_Score=67, Evalue=4e-11,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): SYE_NITMU (Q2Y8L6)

Other databases:

- EMBL:   CP000103
- RefSeq:   YP_412297.1
- ProteinModelPortal:   Q2Y8L6
- SMR:   Q2Y8L6
- STRING:   Q2Y8L6
- GeneID:   3784835
- GenomeReviews:   CP000103_GR
- KEGG:   nmu:Nmul_A1604
- eggNOG:   COG0008
- HOGENOM:   HBG628189
- OMA:   MAHIPLI
- PhylomeDB:   Q2Y8L6
- ProtClustDB:   PRK01406
- BioCyc:   NMUL323848:NMUL_A1604-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00022_B
- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729
- Gene3D:   G3DSA:1.10.1160.10
- Gene3D:   G3DSA:3.40.50.620
- Gene3D:   G3DSA:1.10.10.350
- PANTHER:   PTHR10119
- PRINTS:   PR00987
- TIGRFAMs:   TIGR00464

Pfam domain/function: PF00749 tRNA-synt_1c; SSF48163 tRNA-synt_bind

EC number: =6.1.1.17

Molecular weight: Translated: 52513; Mature: 52513

Theoretical pI: Translated: 6.05; Mature: 6.05

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: BINDING 239-239

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRTRFAPSPTGYLHIGGARTALFSWAYARKHGGKFVLRIEDTDLERSTAQSTQAILDGM
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
AWLGLDYDEGPFYQMQRLARYHEVAEQLLRRGQAYYCYCSREELDAMREQQRAAGLKPRY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCC
DGRWRDSREEPPAGVKPVVRLKNPLDGEVTFKDLIKGEITVANSELDDLVLLRGDGVPTY
CCCCCCCCCCCCCCCCCHHEECCCCCCCEEHHHHHCCCEEEECCCCCCEEEEECCCCCEE
NFGVVIDDLDMNITHVIRGDDHVNNTPRQINILKALGAPLPQYAHVPMILGADGERLSKR
ECCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
HGAVSVMQYREDGYLPEALVNYLARLGWSHGDEEIFSREQLVEWFDLSNINRSPAKFNPE
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
KLQWLNQQYLKTADNERLAELVKPFLAADGCDVTGGGTPDLRKVMNLLKERVNTIAELAD
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAVYFFRPLEPAEELRAQYFSAEAKGPILDLRTKLATLEWEGHAINDAIKASATAHGVKM
HHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCC
PKVAMPLRVMVTGEAQTPAINAVLELLGREETLRRMDRQLEYFLS
CCHHCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIRTRFAPSPTGYLHIGGARTALFSWAYARKHGGKFVLRIEDTDLERSTAQSTQAILDGM
CCCCCCCCCCCCEEEECCHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHH
AWLGLDYDEGPFYQMQRLARYHEVAEQLLRRGQAYYCYCSREELDAMREQQRAAGLKPRY
HHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCCC
DGRWRDSREEPPAGVKPVVRLKNPLDGEVTFKDLIKGEITVANSELDDLVLLRGDGVPTY
CCCCCCCCCCCCCCCCCHHEECCCCCCCEEHHHHHCCCEEEECCCCCCEEEEECCCCCEE
NFGVVIDDLDMNITHVIRGDDHVNNTPRQINILKALGAPLPQYAHVPMILGADGERLSKR
ECCEEEECCCCCEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCCCEEECCCCHHHHHH
HGAVSVMQYREDGYLPEALVNYLARLGWSHGDEEIFSREQLVEWFDLSNINRSPAKFNPE
CCCCEEEEECCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
KLQWLNQQYLKTADNERLAELVKPFLAADGCDVTGGGTPDLRKVMNLLKERVNTIAELAD
HHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AAVYFFRPLEPAEELRAQYFSAEAKGPILDLRTKLATLEWEGHAINDAIKASATAHGVKM
HHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHEEEECCCCHHHHHHHHHHHCCCCC
PKVAMPLRVMVTGEAQTPAINAVLELLGREETLRRMDRQLEYFLS
CCHHCCEEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA