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The map label for this gene is pyk1 [H]

Identifier: 82702636

GI number: 82702636

Start: 1723300

End: 1724406

Strand: Reverse

Name: pyk1 [H]

Synonym: Nmul_A1507

Alternate gene names: 82702636

Gene position: 1724406-1723300 (Counterclockwise)

Preceding gene: 82702637

Following gene: 82702635

Centisome position: 54.15

GC content: 57.45

Gene sequence:

>1107_bases
ATGACCCCCTGGCATCGAGCCAAGATTGTTTGTACGCTGGGTCCCGCAACGGATGCACCCGGAGTTATCGAAGGTCTGAT
CAGCGGAGGAATGGATGTCGCTCGCGTCAATGCTTCGCATAGCGATTCCATCACGCATTCCCGCCGCATCGAACAAGTTC
GCCGGGCGGCAAGTGAGGCTGGGCAACCGGTAGCTATTCTTGTCGATCTACCCGGTCCAAAGTTTCGTCTTGGCAATCTG
GCCGATGACTGTTGCAGACTGGCTGAAGGCGCTGTCGTTACGCTTGTGGAAGAGGGCAGGGCGCTAGAGGCGGTTACTGA
TGATATGGACGGCATCAGGTTGCCGGTGCGCAACCCCGAATTACTCCAGGCGCTGCGTGCAGGGGAACGGGTATTTCTGG
CTGACGGCTCGGTCGAGCTTTGTGTAGAGAGCAGTGCAACAGCTCAGTCTGGATTCAACGCCCCCCGAGTCCGGTGTGAA
GTTCTTATTGGCGGTGTCGTGCGCTCCGGCTCCGGTATCAATGTTCCTGAATCCGCCCTGCTTGAGCTCGTTCCGACTGA
CGATGACCGTCAGCATCTTGCCTTTGCGCTTGCCCGCCAGGTGGAATGGATAGGAATTTCCTTTGTTCAGTCCGTTGACG
AGATCCACCGTGTGCGTGCGTTGCTTCCTTCCACTGGTGCACCGCTCGTGATGGCAAAGATAGAAAAGCGCAAGGCACTC
GTCAATCTGGAGGAGATTGTGGAGGCAGCCGATGGGGTAATGGTGGCGCGGGGAGATTTGGGTGTGGAAACCGATCTTGC
CGAAATTGCGCTGATACAGAAGCGGATTATATCGGTAGCGAACGCTCGGGCACGTCCCGTGATCACCGCTACCCAGATGC
TCGAATCCATGGTGGCGTACGAGCATCCGACACGTGCCGAGGTAACCGATGTGGCCAATGCGGTGCTGGATGGCACGGAC
GCGGTAATGCTTTCCGCTGAAACGGCAATCGGACAGTTTCCCGTCACCGCTGTGAAGATTCTTCAGCGGGTGATTGCCGC
TACTGAAGCAAGGCATAACGGATCCCCCAACTCGAAGATGCGGGCTGGTATGACAGGCGTGCGCTGA

Upstream 100 bases:

>100_bases
TTTCTTGCATTTGTCATTTTACATCTCGTGCCCAAAATCCTGCCAAAAGGCACACCGCATTAGGTGACCGAGTGGAACCC
GATCGATTGGAGTACCTTCT

Downstream 100 bases:

>100_bases
ATGATGTGCATTCTGTGAGCATATCAGCCTCGAAACAGGATAATCTTGGCTTTTCCGCATGTGAACTTGCAGTTCGTATG
GGCGCGCGGGCTGTGGTTGT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK 1 [H]

Number of amino acids: Translated: 368; Mature: 367

Protein sequence:

>368_residues
MTPWHRAKIVCTLGPATDAPGVIEGLISGGMDVARVNASHSDSITHSRRIEQVRRAASEAGQPVAILVDLPGPKFRLGNL
ADDCCRLAEGAVVTLVEEGRALEAVTDDMDGIRLPVRNPELLQALRAGERVFLADGSVELCVESSATAQSGFNAPRVRCE
VLIGGVVRSGSGINVPESALLELVPTDDDRQHLAFALARQVEWIGISFVQSVDEIHRVRALLPSTGAPLVMAKIEKRKAL
VNLEEIVEAADGVMVARGDLGVETDLAEIALIQKRIISVANARARPVITATQMLESMVAYEHPTRAEVTDVANAVLDGTD
AVMLSAETAIGQFPVTAVKILQRVIAATEARHNGSPNSKMRAGMTGVR

Sequences:

>Translated_368_residues
MTPWHRAKIVCTLGPATDAPGVIEGLISGGMDVARVNASHSDSITHSRRIEQVRRAASEAGQPVAILVDLPGPKFRLGNL
ADDCCRLAEGAVVTLVEEGRALEAVTDDMDGIRLPVRNPELLQALRAGERVFLADGSVELCVESSATAQSGFNAPRVRCE
VLIGGVVRSGSGINVPESALLELVPTDDDRQHLAFALARQVEWIGISFVQSVDEIHRVRALLPSTGAPLVMAKIEKRKAL
VNLEEIVEAADGVMVARGDLGVETDLAEIALIQKRIISVANARARPVITATQMLESMVAYEHPTRAEVTDVANAVLDGTD
AVMLSAETAIGQFPVTAVKILQRVIAATEARHNGSPNSKMRAGMTGVR
>Mature_367_residues
TPWHRAKIVCTLGPATDAPGVIEGLISGGMDVARVNASHSDSITHSRRIEQVRRAASEAGQPVAILVDLPGPKFRLGNLA
DDCCRLAEGAVVTLVEEGRALEAVTDDMDGIRLPVRNPELLQALRAGERVFLADGSVELCVESSATAQSGFNAPRVRCEV
LIGGVVRSGSGINVPESALLELVPTDDDRQHLAFALARQVEWIGISFVQSVDEIHRVRALLPSTGAPLVMAKIEKRKALV
NLEEIVEAADGVMVARGDLGVETDLAEIALIQKRIISVANARARPVITATQMLESMVAYEHPTRAEVTDVANAVLDGTDA
VMLSAETAIGQFPVTAVKILQRVIAATEARHNGSPNSKMRAGMTGVR

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pyruvate kinase family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=348, Percent_Identity=35.9195402298851, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI33286420, Length=348, Percent_Identity=35.9195402298851, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI33286418, Length=348, Percent_Identity=35.9195402298851, Blast_Score=203, Evalue=2e-52,
Organism=Homo sapiens, GI10835121, Length=352, Percent_Identity=35.5113636363636, Blast_Score=184, Evalue=9e-47,
Organism=Homo sapiens, GI32967597, Length=352, Percent_Identity=35.5113636363636, Blast_Score=184, Evalue=1e-46,
Organism=Homo sapiens, GI310128732, Length=294, Percent_Identity=30.952380952381, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI310128730, Length=294, Percent_Identity=30.952380952381, Blast_Score=136, Evalue=3e-32,
Organism=Homo sapiens, GI310128736, Length=242, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI310128734, Length=242, Percent_Identity=27.2727272727273, Blast_Score=95, Evalue=1e-19,
Organism=Homo sapiens, GI310128738, Length=225, Percent_Identity=26.6666666666667, Blast_Score=81, Evalue=2e-15,
Organism=Escherichia coli, GI1787965, Length=373, Percent_Identity=34.3163538873995, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1788160, Length=352, Percent_Identity=38.3522727272727, Blast_Score=190, Evalue=1e-49,
Organism=Caenorhabditis elegans, GI17544584, Length=353, Percent_Identity=37.1104815864023, Blast_Score=221, Evalue=4e-58,
Organism=Caenorhabditis elegans, GI71984406, Length=348, Percent_Identity=38.5057471264368, Blast_Score=200, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17506829, Length=348, Percent_Identity=38.5057471264368, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI17506831, Length=348, Percent_Identity=38.5057471264368, Blast_Score=199, Evalue=1e-51,
Organism=Caenorhabditis elegans, GI71984413, Length=348, Percent_Identity=38.5057471264368, Blast_Score=199, Evalue=1e-51,
Organism=Saccharomyces cerevisiae, GI6319279, Length=339, Percent_Identity=31.858407079646, Blast_Score=188, Evalue=1e-48,
Organism=Saccharomyces cerevisiae, GI6324923, Length=340, Percent_Identity=33.5294117647059, Blast_Score=182, Evalue=1e-46,
Organism=Drosophila melanogaster, GI28571814, Length=352, Percent_Identity=35.5113636363636, Blast_Score=208, Evalue=6e-54,
Organism=Drosophila melanogaster, GI24648964, Length=352, Percent_Identity=35.5113636363636, Blast_Score=207, Evalue=7e-54,
Organism=Drosophila melanogaster, GI24648966, Length=334, Percent_Identity=37.7245508982036, Blast_Score=196, Evalue=2e-50,
Organism=Drosophila melanogaster, GI24581235, Length=344, Percent_Identity=31.1046511627907, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24646914, Length=168, Percent_Identity=41.0714285714286, Blast_Score=137, Evalue=8e-33,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 39142; Mature: 39011

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPWHRAKIVCTLGPATDAPGVIEGLISGGMDVARVNASHSDSITHSRRIEQVRRAASEA
CCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHC
GQPVAILVDLPGPKFRLGNLADDCCRLAEGAVVTLVEEGRALEAVTDDMDGIRLPVRNPE
CCCEEEEEECCCCCEEECCCHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHH
LLQALRAGERVFLADGSVELCVESSATAQSGFNAPRVRCEVLIGGVVRSGSGINVPESAL
HHHHHHCCCEEEEECCCEEEEEECCCCHHCCCCCCCEEEEEEEEHHEECCCCCCCCHHHH
LELVPTDDDRQHLAFALARQVEWIGISFVQSVDEIHRVRALLPSTGAPLVMAKIEKRKAL
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
VNLEEIVEAADGVMVARGDLGVETDLAEIALIQKRIISVANARARPVITATQMLESMVAY
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EHPTRAEVTDVANAVLDGTDAVMLSAETAIGQFPVTAVKILQRVIAATEARHNGSPNSKM
CCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
RAGMTGVR
HCCCCCCC
>Mature Secondary Structure 
TPWHRAKIVCTLGPATDAPGVIEGLISGGMDVARVNASHSDSITHSRRIEQVRRAASEA
CCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHC
GQPVAILVDLPGPKFRLGNLADDCCRLAEGAVVTLVEEGRALEAVTDDMDGIRLPVRNPE
CCCEEEEEECCCCCEEECCCHHHHHHHHCCCEEEEECCCCCHHHHHCCCCCCCCCCCCHH
LLQALRAGERVFLADGSVELCVESSATAQSGFNAPRVRCEVLIGGVVRSGSGINVPESAL
HHHHHHCCCEEEEECCCEEEEEECCCCHHCCCCCCCEEEEEEEEHHEECCCCCCCCHHHH
LELVPTDDDRQHLAFALARQVEWIGISFVQSVDEIHRVRALLPSTGAPLVMAKIEKRKAL
EEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
VNLEEIVEAADGVMVARGDLGVETDLAEIALIQKRIISVANARARPVITATQMLESMVAY
CCHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
EHPTRAEVTDVANAVLDGTDAVMLSAETAIGQFPVTAVKILQRVIAATEARHNGSPNSKM
CCCCCHHHHHHHHHHHCCCCEEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCHH
RAGMTGVR
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]