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The map label for this gene is mdeA [H]

Identifier: 82702626

GI number: 82702626

Start: 1712233

End: 1713522

Strand: Reverse

Name: mdeA [H]

Synonym: Nmul_A1497

Alternate gene names: 82702626

Gene position: 1713522-1712233 (Counterclockwise)

Preceding gene: 82702629

Following gene: 82702624

Centisome position: 53.81

GC content: 52.33

Gene sequence:

>1290_bases
ATGGAAGAAAATGGCATGAAACTGGAATCTCTGGCATTGCATTACGGATATAAATCAGAAGCTTCGACGAAGGCCGCGGC
AGTACCGATTTATCAGACGACTTCCTATACATTTGACAATACGCAGCATGGGGCCGACCTGTTTGACCTGAAGGTGGCGG
GAAATATTTATACCCGGATAATGAATCCAACCAATGCAGTGCTGGAGCAGCGTCTGGCAGAGATGGAGGGCGGAGTGGGT
GGGCTGGCAGTTGCTTCAGGTATGGCGGCAATCACTTACGCCATCCAGTGCATCGCCAATGCCGGCGACAACATTGTGAG
CACCAGCCAACTGTATGGCGGCACGTACAATCTGTTTGCGCATACTTTCCCCAGACAGGGGATCGAGGTGCGCATGGCCT
CACATGAGGATTTCGATGGGATTGAGCAACGTATAGATGCCAAGACCCGTGCAATATTTTGTGAATCGATTGGAAATCCC
TCGGGAAATGTTATCGATATCGAGAAGATCGCTGACATCGCTCATCGGCACGGGGTGCCCCTCATCGTCGACAGTACGGT
TTCCACCCCCTATCTATGTCAACCTTTCAAGCTCGGTGCGGATATTGTCGTGCATTCCCTGACGAAATATATCGGTGGAC
ATGGTACAACAGTCGGAGGAATGATTATCGATTCCGGACAATTCGACTGGGCAAAGCATAAGGACCGCTTTCCCTTGTTG
AACGAGCCTGATCCTTCATATCATGACGTTGTTTATACAGAAGCATTTGAAGCCGCGGCCTTTATCGGACGCTGCCGGGT
TGTGCCATTGCGCAACACGGGGGCAGCGCTCGCGCCCCACAGCGCATTTTTGATACTGCAGGGATTGGAAACGCTGGGCT
TGAGAATGGAGCGGCACTGCGAGAATGCATTGAAGGTGGCGCAGTATCTGGAAGCGCATCCCGTCGTCGAGCGCGTCAAT
TACGCGGGTTTGCCCAGCAGTAAATATCATGAACTGTGCAACAGGATCAGCAAAGGGAAAGCATCCGGCCTGTTGAGCTT
TGAGATCAAGGGTGGAGCGGTGGCCGGCAGCGAGTTTATCGATGCCCTGAAGATGATCCTGCGGCTGGTCAATATCGGCG
ATGCCAAATCGCTAGCCTGTCATCCTGCATCAACCACTCACAGACAGTTGAGCCCTGATGAATTAAAGTCTGCGGGAGTT
TCACCGGGTCTCGTGAGGATATCGGTCGGAATTGAGCATATTGACGACATCATCGGCGATATCGCCCAGGCGCTTGATGC
AATCACCTGA

Upstream 100 bases:

>100_bases
TGCCTGTGCAGCTTCATGCTGCTGCTGATCATGGCGTTTTATGAGATCATCGAGGTCATGGGAAGCTTGAAGATATTTAT
TGTAGTCGGGATAAATCTTT

Downstream 100 bases:

>100_bases
TTTTACGGTGAGTTCAACCAGCCTTCCAGGAGGCCTCCTGAAGCATCGAGATGGAGTGGTCCAGGAAAAGTTCGATTTTT
CGCTTGAGATCGGTTGACAT

Product: O-acetylhomoserine/O-acetylserine sulfhydrylase

Products: NA

Alternate protein names: L-methioninase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MEENGMKLESLALHYGYKSEASTKAAAVPIYQTTSYTFDNTQHGADLFDLKVAGNIYTRIMNPTNAVLEQRLAEMEGGVG
GLAVASGMAAITYAIQCIANAGDNIVSTSQLYGGTYNLFAHTFPRQGIEVRMASHEDFDGIEQRIDAKTRAIFCESIGNP
SGNVIDIEKIADIAHRHGVPLIVDSTVSTPYLCQPFKLGADIVVHSLTKYIGGHGTTVGGMIIDSGQFDWAKHKDRFPLL
NEPDPSYHDVVYTEAFEAAAFIGRCRVVPLRNTGAALAPHSAFLILQGLETLGLRMERHCENALKVAQYLEAHPVVERVN
YAGLPSSKYHELCNRISKGKASGLLSFEIKGGAVAGSEFIDALKMILRLVNIGDAKSLACHPASTTHRQLSPDELKSAGV
SPGLVRISVGIEHIDDIIGDIAQALDAIT

Sequences:

>Translated_429_residues
MEENGMKLESLALHYGYKSEASTKAAAVPIYQTTSYTFDNTQHGADLFDLKVAGNIYTRIMNPTNAVLEQRLAEMEGGVG
GLAVASGMAAITYAIQCIANAGDNIVSTSQLYGGTYNLFAHTFPRQGIEVRMASHEDFDGIEQRIDAKTRAIFCESIGNP
SGNVIDIEKIADIAHRHGVPLIVDSTVSTPYLCQPFKLGADIVVHSLTKYIGGHGTTVGGMIIDSGQFDWAKHKDRFPLL
NEPDPSYHDVVYTEAFEAAAFIGRCRVVPLRNTGAALAPHSAFLILQGLETLGLRMERHCENALKVAQYLEAHPVVERVN
YAGLPSSKYHELCNRISKGKASGLLSFEIKGGAVAGSEFIDALKMILRLVNIGDAKSLACHPASTTHRQLSPDELKSAGV
SPGLVRISVGIEHIDDIIGDIAQALDAIT
>Mature_429_residues
MEENGMKLESLALHYGYKSEASTKAAAVPIYQTTSYTFDNTQHGADLFDLKVAGNIYTRIMNPTNAVLEQRLAEMEGGVG
GLAVASGMAAITYAIQCIANAGDNIVSTSQLYGGTYNLFAHTFPRQGIEVRMASHEDFDGIEQRIDAKTRAIFCESIGNP
SGNVIDIEKIADIAHRHGVPLIVDSTVSTPYLCQPFKLGADIVVHSLTKYIGGHGTTVGGMIIDSGQFDWAKHKDRFPLL
NEPDPSYHDVVYTEAFEAAAFIGRCRVVPLRNTGAALAPHSAFLILQGLETLGLRMERHCENALKVAQYLEAHPVVERVN
YAGLPSSKYHELCNRISKGKASGLLSFEIKGGAVAGSEFIDALKMILRLVNIGDAKSLACHPASTTHRQLSPDELKSAGV
SPGLVRISVGIEHIDDIIGDIAQALDAIT

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG2873

COG function: function code E; O-acetylhomoserine sulfhydrylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=418, Percent_Identity=34.4497607655502, Blast_Score=199, Evalue=5e-51,
Organism=Homo sapiens, GI299473759, Length=418, Percent_Identity=31.1004784688995, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI25453487, Length=417, Percent_Identity=30.4556354916067, Blast_Score=144, Evalue=2e-34,
Organism=Escherichia coli, GI1790375, Length=421, Percent_Identity=31.5914489311164, Blast_Score=196, Evalue=3e-51,
Organism=Escherichia coli, GI1789383, Length=422, Percent_Identity=24.6445497630332, Blast_Score=120, Evalue=2e-28,
Organism=Caenorhabditis elegans, GI71980708, Length=442, Percent_Identity=34.3891402714932, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17538055, Length=418, Percent_Identity=33.9712918660287, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI17560056, Length=419, Percent_Identity=34.1288782816229, Blast_Score=191, Evalue=6e-49,
Organism=Saccharomyces cerevisiae, GI6323333, Length=434, Percent_Identity=47.2350230414747, Blast_Score=373, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319307, Length=379, Percent_Identity=33.245382585752, Blast_Score=181, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6321254, Length=446, Percent_Identity=26.457399103139, Blast_Score=130, Evalue=4e-31,
Organism=Saccharomyces cerevisiae, GI6321170, Length=315, Percent_Identity=28.5714285714286, Blast_Score=104, Evalue=3e-23,
Organism=Saccharomyces cerevisiae, GI6321904, Length=431, Percent_Identity=25.5220417633411, Blast_Score=94, Evalue=5e-20,
Organism=Drosophila melanogaster, GI19922554, Length=418, Percent_Identity=33.7320574162679, Blast_Score=208, Evalue=5e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR006237
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =4.4.1.11 [H]

Molecular weight: Translated: 46236; Mature: 46236

Theoretical pI: Translated: 6.16; Mature: 6.16

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENGMKLESLALHYGYKSEASTKAAAVPIYQTTSYTFDNTQHGADLFDLKVAGNIYTRI
CCCCCCCHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHEEE
MNPTNAVLEQRLAEMEGGVGGLAVASGMAAITYAIQCIANAGDNIVSTSQLYGGTYNLFA
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCHHHHE
HTFPRQGIEVRMASHEDFDGIEQRIDAKTRAIFCESIGNPSGNVIDIEKIADIAHRHGVP
ECCCCCCCEEEECCCCCHHHHHHHHHHHHHEEHHHHCCCCCCCEEEHHHHHHHHHHCCCC
LIVDSTVSTPYLCQPFKLGADIVVHSLTKYIGGHGTTVGGMIIDSGQFDWAKHKDRFPLL
EEEECCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCHHHCCCCCCCC
NEPDPSYHDVVYTEAFEAAAFIGRCRVVPLRNTGAALAPHSAFLILQGLETLGLRMERHC
CCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ENALKVAQYLEAHPVVERVNYAGLPSSKYHELCNRISKGKASGLLSFEIKGGAVAGSEFI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
DALKMILRLVNIGDAKSLACHPASTTHRQLSPDELKSAGVSPGLVRISVGIEHIDDIIGD
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHH
IAQALDAIT
HHHHHHHCC
>Mature Secondary Structure
MEENGMKLESLALHYGYKSEASTKAAAVPIYQTTSYTFDNTQHGADLFDLKVAGNIYTRI
CCCCCCCHHHHHEECCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCHHEEE
MNPTNAVLEQRLAEMEGGVGGLAVASGMAAITYAIQCIANAGDNIVSTSQLYGGTYNLFA
CCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHCCCCHHHHE
HTFPRQGIEVRMASHEDFDGIEQRIDAKTRAIFCESIGNPSGNVIDIEKIADIAHRHGVP
ECCCCCCCEEEECCCCCHHHHHHHHHHHHHEEHHHHCCCCCCCEEEHHHHHHHHHHCCCC
LIVDSTVSTPYLCQPFKLGADIVVHSLTKYIGGHGTTVGGMIIDSGQFDWAKHKDRFPLL
EEEECCCCCCCCCCCHHCCHHHHHHHHHHHHCCCCCEECCEEEECCCCCHHHCCCCCCCC
NEPDPSYHDVVYTEAFEAAAFIGRCRVVPLRNTGAALAPHSAFLILQGLETLGLRMERHC
CCCCCCCCHHHHHHHHHHHHHHHCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
ENALKVAQYLEAHPVVERVNYAGLPSSKYHELCNRISKGKASGLLSFEIKGGAVAGSEFI
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHH
DALKMILRLVNIGDAKSLACHPASTTHRQLSPDELKSAGVSPGLVRISVGIEHIDDIIGD
HHHHHHHHHHCCCCCCCEEECCCCCCCCCCCHHHHHHCCCCCCEEEEEECHHHHHHHHHH
IAQALDAIT
HHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8586629; 9190812; 3365412 [H]