Click here to switch to the map view.

The map label for this gene is ydaP [H]

Identifier: 82702596

GI number: 82702596

Start: 1676221

End: 1677987

Strand: Reverse

Name: ydaP [H]

Synonym: Nmul_A1467

Alternate gene names: 82702596

Gene position: 1677987-1676221 (Counterclockwise)

Preceding gene: 82702597

Following gene: 82702594

Centisome position: 52.7

GC content: 53.99

Gene sequence:

>1767_bases
ATGAAAGAAACGGTCAGCGATTTCCTGGTTCGGCGTTTGTCCGAATGGGGGGTAAAACGTATCTTCGGCCTTCCTGGCGA
CGGCATCAACGGCATTATGGGGGCGATCAATCGAGTTTCAGATAAGCTCGAATTTGTCCAGATCAGGCATGAAGAGATGG
CGGCGTTCATGGCGTGCGCCCACGCCAAGTTCACGGGGGAAGTTGGTATTTGCCTGGCCACCTCAGGCCCGGGAGCTATC
CACCTGCTCAATGGTTTATATGATGCCAAGCTCGATCATCAGCCGGTGGTGGCGATAGTAGGTCAGCAGAAACGTACCGC
TCTGGGGGGCAGCTACCAGCAGGAAGTCGATCTCGTTTCGCTCTTCAAGGATGTGGCGCATGAGTATGTTCATATTGTGA
CAACGCCAGGGCAAGTGCGCCACGTGCTCGATCGCGCTATGCGTATTGCAAAGGCCGAGCATAACGTATGCTGCGTTATT
GTTCCCAATGACATCCAGGACATGGAGTTTGTGAAACCCCCTCACGAACATGGCACGATTTATTCGGGAGCAGGATATCG
ATCTCCCCGTGTGGTTCCAGAGATTGAGGACCTGCAACGGGCCGCGGATGTGCTGAACGATGGGTCGAAGGTGGCGATTC
TGGTTGGCGCGGGGGCACTGAACGCAACAAGTGAAATTCTCCAGGTGGCTGATCTGCTCGGCGCGGGAATTGCCAAGGCG
CTACTGGGAAAAACTGTGGTCCCTGACGATCTGCCTTATGTGACAGGGGCAATCGGCATGCTGGGCACAAAGCCGAGCTA
CAGCATGATGACCGAGTGCGACACACTCCTGATGATCGGCTCCAGCTTTCCCTATTCCGAATTTTTGCCTGAGGAGGGGC
AGGCACGCGGCGTCCAGATCGATATCGATGGACGAATGATGAGCATGCGGTACCCGATGGAGGTAAACCTCGTCGGGAAT
AGTGAGGATACGCTAAAGCTGTTGATACCGTTACTCAAAAGGAAGGAAGACCGTACGTGGCGAAACCGTATCGAGAGCAG
TGTCGACGAGTGGTGGAAGAAAATCGAGGCAAGGGCGATGGAGCCGGCAAATCCCATCAATCCCCAGCGCGTATTCTACG
AATTATCGCCACGGCTTCCGGATAATTGCATTCTCGCAGGCGATTCTGGTTCTTCAACATTCTGGTATGCGCGGGATATT
CGAATCCGTAAAGGCATGATGGCTTCGCTTTCCGGCGGTCTTGCCACGATGGGATCAGCCGTGCCCTATGCAATCGCCGC
TAAATTTGCGCATCCTGACAGGGTGGTAATAGCCGTGACAGGAGATGGCGCGATGCAAATGAACGGCATGAATGAGCTCA
TTACCATCGTCAAATACTGGCGACATTGGAGCGATCCCCGGCTCGTGGTACTGGTTTTGAATAATCGCGATCTGAACCTG
GTAACCTGGGAGCAGAGGGCCACTGAGGGTAATCCGAAATTCGATGCCGCTCAGGATCTTCCCGATGTCCCATACGCAGA
TTATGCAAAATTGATTGGTCTGCACGGTATACGCGTCGACCGTCCAGAAAATATCGCCAGCGCATGGGATTGTGCCTTGA
CCGCAGATCGACCGGTGGTGCTCGAGGCATGTACCGACCCGAACGTACCACCGTTGCCACCCCATATCACTTTCAAACAG
GCGAGAGCCTATGCCTCAGCAATCGTGCAAGGTGATTCAGACTCGAGAGAAATATTCAGGGAGACAGTAAAGCAGATTTT
CGCCTGA

Upstream 100 bases:

>100_bases
AAAAGGCCGGGTTTATGTTGCTACCGTGAGCTACCGCACAATGTTTCGTGCACGTTTATGGGATGTTTGGGAGCAATAAT
ATTCCTAACAGAGTTCGATC

Downstream 100 bases:

>100_bases
TCAATTTCTTATGGTGGGAGCACGGATAATTTGGCTCACAATCCAGTTTTGTAGTGAATGCGTTGTTGGAAACCTGACAC
TCTGCTTCGCTTCCGAGTAG

Product: thiamine pyrophosphate protein

Products: Acetate; CO2; Ubiquinol-8 [C]

Alternate protein names: NA

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MKETVSDFLVRRLSEWGVKRIFGLPGDGINGIMGAINRVSDKLEFVQIRHEEMAAFMACAHAKFTGEVGICLATSGPGAI
HLLNGLYDAKLDHQPVVAIVGQQKRTALGGSYQQEVDLVSLFKDVAHEYVHIVTTPGQVRHVLDRAMRIAKAEHNVCCVI
VPNDIQDMEFVKPPHEHGTIYSGAGYRSPRVVPEIEDLQRAADVLNDGSKVAILVGAGALNATSEILQVADLLGAGIAKA
LLGKTVVPDDLPYVTGAIGMLGTKPSYSMMTECDTLLMIGSSFPYSEFLPEEGQARGVQIDIDGRMMSMRYPMEVNLVGN
SEDTLKLLIPLLKRKEDRTWRNRIESSVDEWWKKIEARAMEPANPINPQRVFYELSPRLPDNCILAGDSGSSTFWYARDI
RIRKGMMASLSGGLATMGSAVPYAIAAKFAHPDRVVIAVTGDGAMQMNGMNELITIVKYWRHWSDPRLVVLVLNNRDLNL
VTWEQRATEGNPKFDAAQDLPDVPYADYAKLIGLHGIRVDRPENIASAWDCALTADRPVVLEACTDPNVPPLPPHITFKQ
ARAYASAIVQGDSDSREIFRETVKQIFA

Sequences:

>Translated_588_residues
MKETVSDFLVRRLSEWGVKRIFGLPGDGINGIMGAINRVSDKLEFVQIRHEEMAAFMACAHAKFTGEVGICLATSGPGAI
HLLNGLYDAKLDHQPVVAIVGQQKRTALGGSYQQEVDLVSLFKDVAHEYVHIVTTPGQVRHVLDRAMRIAKAEHNVCCVI
VPNDIQDMEFVKPPHEHGTIYSGAGYRSPRVVPEIEDLQRAADVLNDGSKVAILVGAGALNATSEILQVADLLGAGIAKA
LLGKTVVPDDLPYVTGAIGMLGTKPSYSMMTECDTLLMIGSSFPYSEFLPEEGQARGVQIDIDGRMMSMRYPMEVNLVGN
SEDTLKLLIPLLKRKEDRTWRNRIESSVDEWWKKIEARAMEPANPINPQRVFYELSPRLPDNCILAGDSGSSTFWYARDI
RIRKGMMASLSGGLATMGSAVPYAIAAKFAHPDRVVIAVTGDGAMQMNGMNELITIVKYWRHWSDPRLVVLVLNNRDLNL
VTWEQRATEGNPKFDAAQDLPDVPYADYAKLIGLHGIRVDRPENIASAWDCALTADRPVVLEACTDPNVPPLPPHITFKQ
ARAYASAIVQGDSDSREIFRETVKQIFA
>Mature_588_residues
MKETVSDFLVRRLSEWGVKRIFGLPGDGINGIMGAINRVSDKLEFVQIRHEEMAAFMACAHAKFTGEVGICLATSGPGAI
HLLNGLYDAKLDHQPVVAIVGQQKRTALGGSYQQEVDLVSLFKDVAHEYVHIVTTPGQVRHVLDRAMRIAKAEHNVCCVI
VPNDIQDMEFVKPPHEHGTIYSGAGYRSPRVVPEIEDLQRAADVLNDGSKVAILVGAGALNATSEILQVADLLGAGIAKA
LLGKTVVPDDLPYVTGAIGMLGTKPSYSMMTECDTLLMIGSSFPYSEFLPEEGQARGVQIDIDGRMMSMRYPMEVNLVGN
SEDTLKLLIPLLKRKEDRTWRNRIESSVDEWWKKIEARAMEPANPINPQRVFYELSPRLPDNCILAGDSGSSTFWYARDI
RIRKGMMASLSGGLATMGSAVPYAIAAKFAHPDRVVIAVTGDGAMQMNGMNELITIVKYWRHWSDPRLVVLVLNNRDLNL
VTWEQRATEGNPKFDAAQDLPDVPYADYAKLIGLHGIRVDRPENIASAWDCALTADRPVVLEACTDPNVPPLPPHITFKQ
ARAYASAIVQGDSDSREIFRETVKQIFA

Specific function: Unknown

COG id: COG0028

COG function: function code EH; Thiamine pyrophosphate-requiring enzymes [acetolactate synthase, pyruvate dehydrogenase (cytochrome), glyoxylate carboligase, phosphonopyruvate decarboxylase]

Gene ontology:

Cell location: Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=580, Percent_Identity=25.1724137931034, Blast_Score=129, Evalue=6e-30,
Organism=Escherichia coli, GI1787096, Length=582, Percent_Identity=32.3024054982818, Blast_Score=291, Evalue=6e-80,
Organism=Escherichia coli, GI87081685, Length=553, Percent_Identity=28.2097649186257, Blast_Score=181, Evalue=1e-46,
Organism=Escherichia coli, GI1790104, Length=492, Percent_Identity=27.0325203252033, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1786717, Length=556, Percent_Identity=26.2589928057554, Blast_Score=136, Evalue=5e-33,
Organism=Escherichia coli, GI1788716, Length=568, Percent_Identity=21.6549295774648, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17531301, Length=584, Percent_Identity=25.1712328767123, Blast_Score=93, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI17531299, Length=584, Percent_Identity=25.1712328767123, Blast_Score=93, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6323755, Length=568, Percent_Identity=26.9366197183099, Blast_Score=159, Evalue=9e-40,
Organism=Saccharomyces cerevisiae, GI6320816, Length=502, Percent_Identity=22.1115537848606, Blast_Score=88, Evalue=4e-18,
Organism=Drosophila melanogaster, GI19922626, Length=577, Percent_Identity=23.7435008665511, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR000399
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: 1.2.2.2 [C]

Molecular weight: Translated: 64743; Mature: 64743

Theoretical pI: Translated: 6.04; Mature: 6.04

Prosite motif: PS00187 TPP_ENZYMES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKETVSDFLVRRLSEWGVKRIFGLPGDGINGIMGAINRVSDKLEFVQIRHEEMAAFMACA
CCCHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAKFTGEVGICLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQKRTALGGSYQQEVDLVS
HCCCCCCEEEEEEECCCCCEEHHHCHHHCCCCCCCEEEEECCCHHHHCCCCHHHHHHHHH
LFKDVAHEYVHIVTTPGQVRHVLDRAMRIAKAEHNVCCVIVPNDIQDMEFVKPPHEHGTI
HHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCE
YSGAGYRSPRVVPEIEDLQRAADVLNDGSKVAILVGAGALNATSEILQVADLLGAGIAKA
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LLGKTVVPDDLPYVTGAIGMLGTKPSYSMMTECDTLLMIGSSFPYSEFLPEEGQARGVQI
HHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCEEEEECCCCCHHHCCCCCCCCCCEEE
DIDGRMMSMRYPMEVNLVGNSEDTLKLLIPLLKRKEDRTWRNRIESSVDEWWKKIEARAM
EECCEEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EPANPINPQRVFYELSPRLPDNCILAGDSGSSTFWYARDIRIRKGMMASLSGGLATMGSA
CCCCCCCHHHEEEEECCCCCCCEEEECCCCCCEEEEEEEEEHHCCHHHHHCCCHHHHCCC
VPYAIAAKFAHPDRVVIAVTGDGAMQMNGMNELITIVKYWRHWSDPRLVVLVLNNRDLNL
CCHHHHHHCCCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEE
VTWEQRATEGNPKFDAAQDLPDVPYADYAKLIGLHGIRVDRPENIASAWDCALTADRPVV
EEEHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCEECCCCCCHHHHHCEEEECCCCEE
LEACTDPNVPPLPPHITFKQARAYASAIVQGDSDSREIFRETVKQIFA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKETVSDFLVRRLSEWGVKRIFGLPGDGINGIMGAINRVSDKLEFVQIRHEEMAAFMACA
CCCHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAKFTGEVGICLATSGPGAIHLLNGLYDAKLDHQPVVAIVGQQKRTALGGSYQQEVDLVS
HCCCCCCEEEEEEECCCCCEEHHHCHHHCCCCCCCEEEEECCCHHHHCCCCHHHHHHHHH
LFKDVAHEYVHIVTTPGQVRHVLDRAMRIAKAEHNVCCVIVPNDIQDMEFVKPPHEHGTI
HHHHHHHHEEEEEECCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHCCCCCCCCCE
YSGAGYRSPRVVPEIEDLQRAADVLNDGSKVAILVGAGALNATSEILQVADLLGAGIAKA
ECCCCCCCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHH
LLGKTVVPDDLPYVTGAIGMLGTKPSYSMMTECDTLLMIGSSFPYSEFLPEEGQARGVQI
HHCCCCCCCCCCHHHHHHHHCCCCCCCHHHHHCCEEEEECCCCCHHHCCCCCCCCCCEEE
DIDGRMMSMRYPMEVNLVGNSEDTLKLLIPLLKRKEDRTWRNRIESSVDEWWKKIEARAM
EECCEEEEEECCEEEEEECCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
EPANPINPQRVFYELSPRLPDNCILAGDSGSSTFWYARDIRIRKGMMASLSGGLATMGSA
CCCCCCCHHHEEEEECCCCCCCEEEECCCCCCEEEEEEEEEHHCCHHHHHCCCHHHHCCC
VPYAIAAKFAHPDRVVIAVTGDGAMQMNGMNELITIVKYWRHWSDPRLVVLVLNNRDLNL
CCHHHHHHCCCCCEEEEEEECCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCCCEE
VTWEQRATEGNPKFDAAQDLPDVPYADYAKLIGLHGIRVDRPENIASAWDCALTADRPVV
EEEHHHCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCEECCCCCCHHHHHCEEEECCCCEE
LEACTDPNVPPLPPHITFKQARAYASAIVQGDSDSREIFRETVKQIFA
EEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: FAD oxidase,; Lipids site,; Phospholipids; Thiamine diphosphate [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): 87000 [C]

Specific activity: 200

Km value (mM): 0.0022 {lysolecithin}} 0.0017 {lecithin}} 0.0012 {thiamine} 0.03 {sodium} 2 {ferricyanide}} 11 {pyruvate}} 10 {pyruvate}} [C]

Substrates: H2O; Pyruvate; Ubiquinone-8 [C]

Specific reaction: H2O + Pyruvate + Ubiquinone-8 --> Acetate + CO2 + Ubiquinol-8 [C]

General reaction: Oxidative decarboxylation; Redox reaction [C]

Inhibitor: 2, 3-Butanedione; Cu2+; Methyl methanethio sulfonate; N-Ethyl maleimide diphosphate; p-Chloromercuribenzoate; Phenyl glyoxal [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]