Click here to switch to the map view.

The map label for this gene is insB [H]

Identifier: 82544830

GI number: 82544830

Start: 2376974

End: 2377345

Strand: Reverse

Name: insB [H]

Synonym: SBO_2387

Alternate gene names: 82544830

Gene position: 2377345-2376974 (Counterclockwise)

Preceding gene: 82544831

Following gene: 82544829

Centisome position: 52.6

GC content: 55.65

Gene sequence:

>372_bases
ATGTCGCGCCAGTGCACGCATTATGGGCGTTGGCCTCAACACGGTTTTACGTCACTTAAAAAACTCAGGCCGCAGTCGGT
AACCTCGCGCATACAACCGGGCAGTGATGTGATTGTCTGCGCTGAAATGGACGAACAGTGGGGCTACGTCGGTGCTAAAT
CACGTCAGCGCTGGCTGTTTTACGCGTATGACAGGATACGGAGGACGGTTGTGGCGCACGTCTTCGGTGAACGCACTCTG
GCCACACTGGAGCGTCTTCTGAGCCTGCTGTCGGCCTTTGAGGTCGTGGTATGGATGACGGATGGCTGGCCGCTGTATGA
ATCACGCCTGAAGGGAAAGCTGCACGTTATCAGCAAGCGTTACACTCAGTAG

Upstream 100 bases:

>100_bases
GCTCTCATTGCCGTAAAACATGGCAACTACAGTTCACTTACACCGCCTCTCAGCCCGGTACGCACCAGAAAATCATTGAT
ATGGCCATGAATGGCGTCGG

Downstream 100 bases:

>100_bases
ACTGGCCCCCTGAATCTCCAGACAACCAGTATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTG
GAGTACAGATGATTGATGTC

Product: IS1 ORF2

Products: NA

Alternate protein names: IS1a [H]

Number of amino acids: Translated: 123; Mature: 122

Protein sequence:

>123_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQ

Sequences:

>Translated_123_residues
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTL
ATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQ
>Mature_122_residues
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLFYAYDRIRRTVVAHVFGERTLA
TLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKRYTQ

Specific function: Absolutely required for transposition of IS1 [H]

COG id: COG1662

COG function: function code L; Transposase and inactivated derivatives, IS1 family

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transposase 27 family [H]

Homologues:

Organism=Escherichia coli, GI1789853, Length=123, Percent_Identity=87.8048780487805, Blast_Score=231, Evalue=1e-62,
Organism=Escherichia coli, GI1788203, Length=123, Percent_Identity=87.8048780487805, Blast_Score=231, Evalue=1e-62,
Organism=Escherichia coli, GI1786203, Length=123, Percent_Identity=87.8048780487805, Blast_Score=231, Evalue=1e-62,
Organism=Escherichia coli, GI1787223, Length=123, Percent_Identity=86.1788617886179, Blast_Score=228, Evalue=1e-61,
Organism=Escherichia coli, GI1786470, Length=123, Percent_Identity=86.1788617886179, Blast_Score=226, Evalue=3e-61,
Organism=Escherichia coli, GI1786459, Length=123, Percent_Identity=86.1788617886179, Blast_Score=226, Evalue=3e-61,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005063 [H]

Pfam domain/function: PF03400 Transposase_27 [H]

EC number: NA

Molecular weight: Translated: 14449; Mature: 14318

Theoretical pI: Translated: 10.44; Mature: 10.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHCCHHHHHHHH
YTQ
CCC
>Mature Secondary Structure 
SRQCTHYGRWPQHGFTSLKKLRPQSVTSRIQPGSDVIVCAEMDEQWGYVGAKSRQRWLF
CCCCCCCCCCCCHHHHHHHHCCCHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCHHH
YAYDRIRRTVVAHVFGERTLATLERLLSLLSAFEVVVWMTDGWPLYESRLKGKLHVISKR
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHCCHHHHHHHH
YTQ
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 375010; 1849492; 1630901; 9278503 [H]