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The map label for this gene is entE [H]

Identifier: 82543040

GI number: 82543040

Start: 474547

End: 476157

Strand: Direct

Name: entE [H]

Synonym: SBO_0455

Alternate gene names: 82543040

Gene position: 474547-476157 (Clockwise)

Preceding gene: 82543039

Following gene: 82543041

Centisome position: 10.5

GC content: 56.36

Gene sequence:

>1611_bases
ATGAGCATTCCATTCACCCGCTGGCCGGAAGAGTTTGCCCGTCGCTATCGGGAAAAAGGCTACTGGCAGGATTTGCCGCT
GACCGACATTCTGACTCGCCACGCTGCGAGTGACAGCATCGCGGTTATCGACGGCGAGCTACAGTTGAGTTACCGGGAGC
TGAATCAGGCGGCGGATAACCTCGCGTGTACTTTACGCCGTCAGGGCATTAAACCTGGTGAAACCGCGCTGGTACAACTG
GGTAACGTCGCTGAATTGTATATTACCTTTTTCGCGCTGCTGAAACTGGGCGTTGCGCCGGTGCTGGCGTTGTTCAGCCA
TCAGCGTAGTGAACTGAACGCCTATGCCAGCCAGATTGAACCCGCATTGCTGATTGCCGATCGCCAACATGCGCTGTTTA
GCGGGGATGATTTCCTCAATACTTTCGTCACAGAACATTCCTCCATTCGCGTGGTGCAACTGCACAACGACAGCGGTGAG
CATAACTTGCAGGATGCGATTAACCATCCGGCAGACGGTTTTACTGCCACGCCATCACCTGCTGATGAAGTGGCCTATTT
CCAGCTTTCCGGCGGCACCACCGGTACACCGAAGCTGATCCCGCGCACTCATAACGACTACTACTACAGCGTGCGTCGTA
GCGTCGAGATTTGTCAGTTCACACAACAGACGCGCTACCTGTGCGCGATCCCGGCGGCTCATAACTACGCCATGAGTTCG
CCAGGATCGCTGGGCGTCTTTCTTGCCGGAGGAACGGTTGTTCTGGCGGCCGATCCCAGCGCTACGCTTTGCTTCCCATT
GATTGAAAAACATCAGGTGAACGTCACCGCGCTGGTGCCGCCGGCAGTCAGCCTGTGGTTGCAGGCGCTGGCTGAAGGCG
AAAGCCGGGCGCAGCTTGCCTCGCTGAAACTGTTACAGGTCGGCGGCGCACGTCTTTCTGCCACCCTTGCGGCGCGTATT
CCCGCTGAGATTGGCTGCCAGTTGCAGCAGGTGTTTGGCATGGCGGAAGGGCTGGTGAACTACACCCGTCTTGATGATAG
CGCGGAGAAAATTATCCATACCCAGGGTTACCCAATGTGTCCGGACGATGAAGTGTGGGTTGCCGATGCCGAAGGAAATC
CACTGCCGCAAGGGGAAGTCGGACGCCTGATGACGCGCGGGCCGTACACCTTCCGCGGTTATTACAAAAGTCCGCAGCAC
AATGCCAGCGCCTTTGATGCCAACGGTTTTTACTGTTCCGGCGATCTGATCTCTATTGATCCAGAGGGTTACATCACCGT
GCAGGGGCGCGAGAAAGATCAGATCAACCGTGGCGGCGAGAAGATCGCTGCCGAAGAGATCGAAAACCTGCTGCTGCGCC
ATCCGGCGGTGATCTATGCCGCACTGGTGAGCATGGAAGATGAGCTGATGGGCGAAAAAAGCTGTGCTTATCTGGTGGTA
AAAGAGCCGCTGCGCGCGGTGCAGGTGCGTCGTTTCCTGCGTGAACAGGGTATTGCCGAATTTAAATTACCGGATCGCGT
GGAGTGTGTAGATTCACTTCCGCTGACGGCGGTCGGGAAAGTCGATAAAAAACAATTAAGTCAGTGGCTGGCGTCACGCG
CATCAGCCTGA

Upstream 100 bases:

>100_bases
GGGGATTGTGCTTGCGTCGTCACCGTTGGGTGAGTGGCGCGAAACAGGCGTCAAACTTTCTACCATGTTGAATGTTTTTG
GATTGCATTAAGGAGCGAGG

Downstream 100 bases:

>100_bases
AGGAGAGAACACGATGGCTATTCCAAAATTACAGGCTTACGCACTGCCGGAGTCTCACGATATTCCGCAGAATAAAGTTG
ACTGGGCCTTTGAACTGCAA

Product: enterobactin synthase subunit E

Products: NA

Alternate protein names: Enterochelin synthase E; 2,3-dihydroxybenzoate-AMP ligase; Dihydroxybenzoic acid-activating enzyme; S-dihydroxybenzoyltransferase [H]

Number of amino acids: Translated: 536; Mature: 535

Protein sequence:

>536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGELQLSYRELNQAADNLACTLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLSQWLASRASA

Sequences:

>Translated_536_residues
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGELQLSYRELNQAADNLACTLRRQGIKPGETALVQL
GNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGE
HNLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARI
PAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQH
NASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLSQWLASRASA
>Mature_535_residues
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGELQLSYRELNQAADNLACTLRRQGIKPGETALVQLG
NVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIEPALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGEH
NLQDAINHPADGFTATPSPADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSSP
GSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLASLKLLQVGGARLSATLAARIP
AEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMCPDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHN
ASAFDANGFYCSGDLISIDPEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVVK
EPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLSQWLASRASA

Specific function: Activates the carboxylate group of 2,3-dihydroxy- benzoate (2,3-DHB), via ATP-dependent PPi exchange reactions, to the acyladenylate. Then, catalyzes the acylation of holo-EntB with 2,3-DHB adenylate, preparing that molecule for amide bond formation with

COG id: COG1021

COG function: function code Q; Peptide arylation enzymes

Gene ontology:

Cell location: Integral Membrane Protein. Inner Membrane [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. EntE subfamily [H]

Homologues:

Organism=Homo sapiens, GI38505220, Length=504, Percent_Identity=23.8095238095238, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI122937307, Length=509, Percent_Identity=23.5756385068762, Blast_Score=107, Evalue=3e-23,
Organism=Homo sapiens, GI42544132, Length=510, Percent_Identity=22.3529411764706, Blast_Score=104, Evalue=2e-22,
Organism=Homo sapiens, GI157311624, Length=517, Percent_Identity=23.5976789168279, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI157311622, Length=517, Percent_Identity=23.5976789168279, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI156151445, Length=189, Percent_Identity=31.2169312169312, Blast_Score=100, Evalue=4e-21,
Organism=Homo sapiens, GI58082049, Length=517, Percent_Identity=23.9845261121857, Blast_Score=99, Evalue=1e-20,
Organism=Homo sapiens, GI187761345, Length=402, Percent_Identity=24.3781094527363, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI187761343, Length=402, Percent_Identity=24.3781094527363, Blast_Score=93, Evalue=5e-19,
Organism=Homo sapiens, GI115511026, Length=508, Percent_Identity=22.244094488189, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI28416953, Length=531, Percent_Identity=21.6572504708098, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI57165412, Length=382, Percent_Identity=24.3455497382199, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI57165410, Length=382, Percent_Identity=23.5602094240838, Blast_Score=75, Evalue=2e-13,
Organism=Homo sapiens, GI8923896, Length=547, Percent_Identity=20.109689213894, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1786810, Length=536, Percent_Identity=98.6940298507463, Blast_Score=1092, Evalue=0.0,
Organism=Escherichia coli, GI145693145, Length=532, Percent_Identity=31.015037593985, Blast_Score=221, Evalue=1e-58,
Organism=Escherichia coli, GI221142682, Length=491, Percent_Identity=26.4765784114053, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI1788107, Length=536, Percent_Identity=25.1865671641791, Blast_Score=112, Evalue=7e-26,
Organism=Escherichia coli, GI1790505, Length=540, Percent_Identity=24.2592592592593, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1786801, Length=562, Percent_Identity=24.9110320284698, Blast_Score=92, Evalue=9e-20,
Organism=Escherichia coli, GI1786529, Length=526, Percent_Identity=23.1939163498099, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789201, Length=360, Percent_Identity=22.5, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17557194, Length=539, Percent_Identity=26.7161410018553, Blast_Score=134, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17538037, Length=547, Percent_Identity=26.508226691042, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71994703, Length=524, Percent_Identity=23.4732824427481, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994694, Length=524, Percent_Identity=23.4732824427481, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI32563687, Length=500, Percent_Identity=23, Blast_Score=101, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI71994690, Length=524, Percent_Identity=23.4732824427481, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17560308, Length=363, Percent_Identity=26.4462809917355, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI71996755, Length=525, Percent_Identity=23.6190476190476, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17558820, Length=504, Percent_Identity=23.4126984126984, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI71985884, Length=358, Percent_Identity=27.0949720670391, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17531443, Length=369, Percent_Identity=23.0352303523035, Blast_Score=89, Evalue=7e-18,
Organism=Caenorhabditis elegans, GI17559526, Length=531, Percent_Identity=22.0338983050847, Blast_Score=76, Evalue=5e-14,
Organism=Caenorhabditis elegans, GI17560140, Length=515, Percent_Identity=23.3009708737864, Blast_Score=75, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6319699, Length=539, Percent_Identity=23.0055658627087, Blast_Score=126, Evalue=9e-30,
Organism=Saccharomyces cerevisiae, GI6319264, Length=540, Percent_Identity=22.4074074074074, Blast_Score=84, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24581924, Length=521, Percent_Identity=23.9923224568138, Blast_Score=125, Evalue=9e-29,
Organism=Drosophila melanogaster, GI19922652, Length=494, Percent_Identity=24.0890688259109, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24656500, Length=530, Percent_Identity=24.7169811320755, Blast_Score=91, Evalue=2e-18,
Organism=Drosophila melanogaster, GI21355181, Length=500, Percent_Identity=22.8, Blast_Score=83, Evalue=5e-16,
Organism=Drosophila melanogaster, GI21356947, Length=417, Percent_Identity=23.5011990407674, Blast_Score=80, Evalue=4e-15,
Organism=Drosophila melanogaster, GI18859661, Length=370, Percent_Identity=24.8648648648649, Blast_Score=79, Evalue=9e-15,
Organism=Drosophila melanogaster, GI24648260, Length=270, Percent_Identity=26.6666666666667, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24648253, Length=512, Percent_Identity=22.0703125, Blast_Score=74, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24667955, Length=564, Percent_Identity=21.6312056737589, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI24648255, Length=499, Percent_Identity=22.4448897795591, Blast_Score=73, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21356441, Length=443, Percent_Identity=21.2189616252822, Blast_Score=72, Evalue=6e-13,
Organism=Drosophila melanogaster, GI62472339, Length=535, Percent_Identity=21.4953271028037, Blast_Score=72, Evalue=7e-13,
Organism=Drosophila melanogaster, GI24667959, Length=374, Percent_Identity=23.2620320855615, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI21358303, Length=499, Percent_Identity=22.4448897795591, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI281365686, Length=329, Percent_Identity=25.531914893617, Blast_Score=70, Evalue=3e-12,
Organism=Drosophila melanogaster, GI20130357, Length=441, Percent_Identity=22.4489795918367, Blast_Score=67, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873
- InterPro:   IPR011963 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =2.7.7.58 [H]

Molecular weight: Translated: 58924; Mature: 58793

Theoretical pI: Translated: 5.34; Mature: 5.34

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGELQLSYRELNQAADN
CCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCEEEHHHHHHHHHHH
LACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGEHNLQDAINHPADGFTATPSP
CEEEEECCCCCEECCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLSQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
SIPFTRWPEEFARRYREKGYWQDLPLTDILTRHAASDSIAVIDGELQLSYRELNQAADN
CCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCEEEEECCEEEHHHHHHHHHHH
LACTLRRQGIKPGETALVQLGNVAELYITFFALLKLGVAPVLALFSHQRSELNAYASQIE
HHHHHHHCCCCCCCHHHEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
PALLIADRQHALFSGDDFLNTFVTEHSSIRVVQLHNDSGEHNLQDAINHPADGFTATPSP
CEEEEECCCCCEECCHHHHHHHHCCCCCEEEEEEECCCCCCHHHHHHCCCCCCCCCCCCC
ADEVAYFQLSGGTTGTPKLIPRTHNDYYYSVRRSVEICQFTQQTRYLCAIPAAHNYAMSS
CCCEEEEEECCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
PGSLGVFLAGGTVVLAADPSATLCFPLIEKHQVNVTALVPPAVSLWLQALAEGESRAQLA
CCCEEEEEECCEEEEEECCCCEEEHHHHHCCCCEEEEECCHHHHHHHHHHHCCCHHHHHH
SLKLLQVGGARLSATLAARIPAEIGCQLQQVFGMAEGLVNYTRLDDSAEKIIHTQGYPMC
HHHHHHHCCCHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCC
PDDEVWVADAEGNPLPQGEVGRLMTRGPYTFRGYYKSPQHNASAFDANGFYCSGDLISID
CCCCEEEECCCCCCCCCCCHHHHHCCCCCEEECCCCCCCCCCCEECCCCEEECCCEEEEC
PEGYITVQGREKDQINRGGEKIAAEEIENLLLRHPAVIYAALVSMEDELMGEKSCAYLVV
CCCEEEEECCCHHHHHCCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCEEEEEE
KEPLRAVQVRRFLREQGIAEFKLPDRVECVDSLPLTAVGKVDKKQLSQWLASRASA
HHHHHHHHHHHHHHHCCCEECCCCCHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]