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The map label for this gene is trmD

Identifier: 78779760

GI number: 78779760

Start: 1290128

End: 1290865

Strand: Reverse

Name: trmD

Synonym: PMT9312_1376

Alternate gene names: 78779760

Gene position: 1290865-1290128 (Counterclockwise)

Preceding gene: 78779761

Following gene: 78779759

Centisome position: 75.52

GC content: 32.66

Gene sequence:

>738_bases
ATGAATAGTTTTAATATTGATGTAATTACATTATTACCTAAAGCTTTTGAATTAATTAAGAATTTAGGTGTTATAACAAG
AGCTCTAGATAAGAATTTGATTAATGTTAATTTACATGATTTAAGAGAATATGGAGAAGGTTCTTACAGACAAGTAGACG
ATAAGCCTTATGGCGGAGGAGCAGGAATGGTCTTAAAACCTGGCCCTATTTTTAAAGCGCATGAATCAATTAATAAATTT
CCTAAAAGTAAAACTTTATTGATGACCCCACAGGGTAAAGTCTTAAAGCAAAAGGATTTTGTGAGATGGTCAACTTTGGA
TCAAATAATAATTATTTGTGGTCAATATGAAGGTTTTGATGAAAGGGTTAGATGCTTAGCTGATGAGGAGATATCTATAG
GAGATTATGTACTTTCTGGAGGAGAAATTCCTGCGTTGTCAATAATTAACGGTTTGACCAGATTATTGCCAGGAACACTT
GGGGATCCAGATTCTTTGGTTAATGAGAGTCATAACTCACCTTTATTAGAGCACCCACAGTATACTAGACCTCAAATTTT
TAGGGATATGAAAGTTCCAGATGTTCTCATTAATGGTAATCATAAAGAAATTGAACTTTGGAGAGAAGAGCGGATGTTAA
AAAGAACAATTAAAAGAAGAAAAGATCTAATTAAAAATGAATTTTGTGATTTGCCAATCGATGAATATGATGGAGATGAT
TGGCTCTGGGATTTATAG

Upstream 100 bases:

>100_bases
TAATGGCCCAGTTTACTTGGAGTTGTTTGTGAAAGTTGTTCCAAATTGGAGAAAAAAAGAATCAAGGCTACTTGAGTTTG
GTTATGAGGAAGATTTCTAG

Downstream 100 bases:

>100_bases
GAAAGTTACTTCAGATATTATTTAAAAAAGATGATAAATTAAATAATTCTTCTCTAAATATCTCTAAAGATTAATTTTCT
ATTTTTCTAGATTAATAGTA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 245; Mature: 245

Protein sequence:

>245_residues
MNSFNIDVITLLPKAFELIKNLGVITRALDKNLINVNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPGPIFKAHESINKF
PKSKTLLMTPQGKVLKQKDFVRWSTLDQIIIICGQYEGFDERVRCLADEEISIGDYVLSGGEIPALSIINGLTRLLPGTL
GDPDSLVNESHNSPLLEHPQYTRPQIFRDMKVPDVLINGNHKEIELWREERMLKRTIKRRKDLIKNEFCDLPIDEYDGDD
WLWDL

Sequences:

>Translated_245_residues
MNSFNIDVITLLPKAFELIKNLGVITRALDKNLINVNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPGPIFKAHESINKF
PKSKTLLMTPQGKVLKQKDFVRWSTLDQIIIICGQYEGFDERVRCLADEEISIGDYVLSGGEIPALSIINGLTRLLPGTL
GDPDSLVNESHNSPLLEHPQYTRPQIFRDMKVPDVLINGNHKEIELWREERMLKRTIKRRKDLIKNEFCDLPIDEYDGDD
WLWDL
>Mature_245_residues
MNSFNIDVITLLPKAFELIKNLGVITRALDKNLINVNLHDLREYGEGSYRQVDDKPYGGGAGMVLKPGPIFKAHESINKF
PKSKTLLMTPQGKVLKQKDFVRWSTLDQIIIICGQYEGFDERVRCLADEEISIGDYVLSGGEIPALSIINGLTRLLPGTL
GDPDSLVNESHNSPLLEHPQYTRPQIFRDMKVPDVLINGNHKEIELWREERMLKRTIKRRKDLIKNEFCDLPIDEYDGDD
WLWDL

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=224, Percent_Identity=40.625, Blast_Score=177, Evalue=4e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_PROM9 (Q319K9)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397872.1
- ProteinModelPortal:   Q319K9
- SMR:   Q319K9
- STRING:   Q319K9
- GeneID:   3766188
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1376
- eggNOG:   COG0336
- HOGENOM:   HBG285805
- OMA:   NRYDLYL
- ProtClustDB:   PRK00026
- BioCyc:   PMAR74546:PMT9312_1376-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 28032; Mature: 28032

Theoretical pI: Translated: 5.43; Mature: 5.43

Prosite motif: NA

Important sites: BINDING 114-114

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSFNIDVITLLPKAFELIKNLGVITRALDKNLINVNLHDLREYGEGSYRQVDDKPYGGG
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCEECCCCCCCCC
AGMVLKPGPIFKAHESINKFPKSKTLLMTPQGKVLKQKDFVRWSTLDQIIIICGQYEGFD
CCEEECCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCHH
ERVRCLADEEISIGDYVLSGGEIPALSIINGLTRLLPGTLGDPDSLVNESHNSPLLEHPQ
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
YTRPQIFRDMKVPDVLINGNHKEIELWREERMLKRTIKRRKDLIKNEFCDLPIDEYDGDD
CCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
WLWDL
EEECC
>Mature Secondary Structure
MNSFNIDVITLLPKAFELIKNLGVITRALDKNLINVNLHDLREYGEGSYRQVDDKPYGGG
CCCCCEEHHHHHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCEECCCCCCCCC
AGMVLKPGPIFKAHESINKFPKSKTLLMTPQGKVLKQKDFVRWSTLDQIIIICGQYEGFD
CCEEECCCCCHHHHHHHHHCCCCCEEEECCCCCHHHHHHHHHHHHHCEEEEEECCCCCHH
ERVRCLADEEISIGDYVLSGGEIPALSIINGLTRLLPGTLGDPDSLVNESHNSPLLEHPQ
HHHHHHHCCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCCCCHHHHHCCCCCCCCCCCCC
YTRPQIFRDMKVPDVLINGNHKEIELWREERMLKRTIKRRKDLIKNEFCDLPIDEYDGDD
CCCHHHHHCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCC
WLWDL
EEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA