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The map label for this gene is ispF

Identifier: 78779758

GI number: 78779758

Start: 1288084

End: 1288587

Strand: Reverse

Name: ispF

Synonym: PMT9312_1374

Alternate gene names: 78779758

Gene position: 1288587-1288084 (Counterclockwise)

Preceding gene: 78779759

Following gene: 78779757

Centisome position: 75.39

GC content: 30.56

Gene sequence:

>504_bases
ATGAACGATTTCACTCCAAAATTTAGAATTGGTAATGGATATGATATTCATAGATTAGTAACAGGAAGAAAACTTATTAT
AGGTGGAGTTAATTTAAAACATCCGGATAATTTGGGGTTAGATGGTCATAGTGATGCAGATGTGTTAACTCATTCAATAA
TGGATGCATTATTAGGAGCATTGTCTCTAGGAGACATAGGAAAATATTTTCCTCCTTCAGATGACAAATGGAAAGATGTA
GATAGTCTAATTTTGTTATCGAAGGTTATTGATTTAGTTAGAAAGCAAGGTTGGGAAATAAATAATATTGATAGCGTTCT
TGTTGCTGAAAGACCAAAAATTAAACCATTTGTTGAAATAATGAAAAAAAATCTCTCTAATACTCTAAAAATAGATAATA
GTTTTATTGGAATAAAAGCAACTACGAATGAAAAGTTAGGACCAGAAGGTAGAGAAGAAGGAATAAGTTGTCACTCTGTA
GTTTTACTCGAGAAAAAAGAATGA

Upstream 100 bases:

>100_bases
ATTGAGCTAATAGTAATTATGTATTAATTATTTCTGTGAAAAATTTATTAGAAATTAAAGAATAATCACAGAGTTTATTT
CGATTACAAAACCGGATAAA

Downstream 100 bases:

>100_bases
AAATAAATTCTAATTTTAAAAAGAGAATAAAAAGCTTTTTTTTCTTATTTTTCTCTTTATTTGTTTTTCTTAATCAAATT
AATACTCATATTACTGAAGC

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS

Number of amino acids: Translated: 167; Mature: 167

Protein sequence:

>167_residues
MNDFTPKFRIGNGYDIHRLVTGRKLIIGGVNLKHPDNLGLDGHSDADVLTHSIMDALLGALSLGDIGKYFPPSDDKWKDV
DSLILLSKVIDLVRKQGWEINNIDSVLVAERPKIKPFVEIMKKNLSNTLKIDNSFIGIKATTNEKLGPEGREEGISCHSV
VLLEKKE

Sequences:

>Translated_167_residues
MNDFTPKFRIGNGYDIHRLVTGRKLIIGGVNLKHPDNLGLDGHSDADVLTHSIMDALLGALSLGDIGKYFPPSDDKWKDV
DSLILLSKVIDLVRKQGWEINNIDSVLVAERPKIKPFVEIMKKNLSNTLKIDNSFIGIKATTNEKLGPEGREEGISCHSV
VLLEKKE
>Mature_167_residues
MNDFTPKFRIGNGYDIHRLVTGRKLIIGGVNLKHPDNLGLDGHSDADVLTHSIMDALLGALSLGDIGKYFPPSDDKWKDV
DSLILLSKVIDLVRKQGWEINNIDSVLVAERPKIKPFVEIMKKNLSNTLKIDNSFIGIKATTNEKLGPEGREEGISCHSV
VLLEKKE

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family

Homologues:

Organism=Escherichia coli, GI1789103, Length=158, Percent_Identity=42.4050632911392, Blast_Score=118, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISPF_PROM9 (Q319L1)

Other databases:

- EMBL:   CP000111
- RefSeq:   YP_397870.1
- ProteinModelPortal:   Q319L1
- SMR:   Q319L1
- STRING:   Q319L1
- GeneID:   3766186
- GenomeReviews:   CP000111_GR
- KEGG:   pmi:PMT9312_1374
- eggNOG:   COG0245
- HOGENOM:   HBG630070
- OMA:   HKFGGEG
- ProtClustDB:   PRK00084
- BioCyc:   PMAR74546:PMT9312_1374-MONOMER
- HAMAP:   MF_00107
- InterPro:   IPR003526
- InterPro:   IPR020555
- Gene3D:   G3DSA:3.30.1330.50
- TIGRFAMs:   TIGR00151

Pfam domain/function: PF02542 YgbB; SSF69765 YgbB_synth

EC number: =4.6.1.12

Molecular weight: Translated: 18529; Mature: 18529

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDFTPKFRIGNGYDIHRLVTGRKLIIGGVNLKHPDNLGLDGHSDADVLTHSIMDALLGA
CCCCCCCEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDDKWKDVDSLILLSKVIDLVRKQGWEINNIDSVLVAERPKIKPFVEI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHH
MKKNLSNTLKIDNSFIGIKATTNEKLGPEGREEGISCHSVVLLEKKE
HHHHHCCEEEECCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEEECC
>Mature Secondary Structure
MNDFTPKFRIGNGYDIHRLVTGRKLIIGGVNLKHPDNLGLDGHSDADVLTHSIMDALLGA
CCCCCCCEECCCCCCEEEEECCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSLGDIGKYFPPSDDKWKDVDSLILLSKVIDLVRKQGWEINNIDSVLVAERPKIKPFVEI
HHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEECCCCCCCHHHHH
MKKNLSNTLKIDNSFIGIKATTNEKLGPEGREEGISCHSVVLLEKKE
HHHHHCCEEEECCCEEEEEECCCCCCCCCCHHCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA