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The map label for this gene is trpA

Identifier: 78778957

GI number: 78778957

Start: 533472

End: 534314

Strand: Direct

Name: trpA

Synonym: PMT9312_0572

Alternate gene names: 78778957

Gene position: 533472-534314 (Clockwise)

Preceding gene: 78778956

Following gene: 161350037

Centisome position: 31.21

GC content: 33.45

Gene sequence:

>843_bases
ATGAAAGATAACAAAATGCAAATTACTAAAAATGAATCTTTATCAAAGGTAGATGAGAAGTTTTATGAGTTAAAAAAAAA
TAAAAAAATAGCTTTGATGCCTTTCATAATGGCTGGGGATCCCAATATTGAAATAACCTCTGAGATCTTATTAAAGTTAC
AAGAAAATGGAGCGGACCTTATTGAATTAGGCATCCCATACAGTGATCCACTCGCAGACGGACCTGTTATTCAAGTAGCG
GCCTCTCGCGCCTTAAAGTCAGGTACTACCCCAAGAAAAGTAATTACAATTTTAGAGTCTTTAAAAGGTAAATTAAATAT
TCCCATCATACTTTTTTCTTACTTAAATCCCTTACTATGTTTTGGCTTTGAACAGTTTTGTGAGACAGCATCTAATGCTG
GTGTTTCTGGACTAATAATTCCTGATCTACCTTTAGAGGAAGCTTGTAAATTTTCTAAAATAGTTAGCAACCATTCTATG
GACTTGATTTTATTGGTAGCTCCAACTACTCCTTTTGAAAGAATGAAACAAATATCAAATCATACAAAAGGCTTTACTTA
TTTGGTAAGTGTTACAGGTGTCACTGGTGAGAGAAATAAAATGGAAAATAGAGTAGAAAATCTTATCGCTAAATTAAAAG
AAATAAATTCTAATCCAATTGCTGTTGGTTTTGGGATATCAACTCCTGAACATGTTAATAAAGTTCGAGAGTGGGGAGCA
GATGGAGTAATTATTGGGAGCGCATTTGTAAAACGAATTTCTAGTTCAAGTGAAAGAGATGTCGTTGATCATGTGGGTGA
ATTTTGTAAGGATATGCGTTTAGCTGCTGATCAAAAAAAATAA

Upstream 100 bases:

>100_bases
TTGAAAATAAGTTCAATGCATTGTCAAAGGAAGAGCAGGAAAAACTAATGGAAGATTTAGAAAAAAATCCATAAACTTTC
ATAGACAAAATCTAAAATTC

Downstream 100 bases:

>100_bases
ATACAAAGCGAATATATTGATAAAAAAACTAATTAGTAATTTAAAAAATTAAGTATCAAATTTATGTTTGTTAGCTTTAA
GATTCTTCAGTAGGTAATAA

Product: tryptophan synthase subunit alpha

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 280; Mature: 280

Protein sequence:

>280_residues
MKDNKMQITKNESLSKVDEKFYELKKNKKIALMPFIMAGDPNIEITSEILLKLQENGADLIELGIPYSDPLADGPVIQVA
ASRALKSGTTPRKVITILESLKGKLNIPIILFSYLNPLLCFGFEQFCETASNAGVSGLIIPDLPLEEACKFSKIVSNHSM
DLILLVAPTTPFERMKQISNHTKGFTYLVSVTGVTGERNKMENRVENLIAKLKEINSNPIAVGFGISTPEHVNKVREWGA
DGVIIGSAFVKRISSSSERDVVDHVGEFCKDMRLAADQKK

Sequences:

>Translated_280_residues
MKDNKMQITKNESLSKVDEKFYELKKNKKIALMPFIMAGDPNIEITSEILLKLQENGADLIELGIPYSDPLADGPVIQVA
ASRALKSGTTPRKVITILESLKGKLNIPIILFSYLNPLLCFGFEQFCETASNAGVSGLIIPDLPLEEACKFSKIVSNHSM
DLILLVAPTTPFERMKQISNHTKGFTYLVSVTGVTGERNKMENRVENLIAKLKEINSNPIAVGFGISTPEHVNKVREWGA
DGVIIGSAFVKRISSSSERDVVDHVGEFCKDMRLAADQKK
>Mature_280_residues
MKDNKMQITKNESLSKVDEKFYELKKNKKIALMPFIMAGDPNIEITSEILLKLQENGADLIELGIPYSDPLADGPVIQVA
ASRALKSGTTPRKVITILESLKGKLNIPIILFSYLNPLLCFGFEQFCETASNAGVSGLIIPDLPLEEACKFSKIVSNHSM
DLILLVAPTTPFERMKQISNHTKGFTYLVSVTGVTGERNKMENRVENLIAKLKEINSNPIAVGFGISTPEHVNKVREWGA
DGVIIGSAFVKRISSSSERDVVDHVGEFCKDMRLAADQKK

Specific function: The alpha subunit is responsible for the aldol cleavage of indoleglycerol phosphate to indole and glyceraldehyde 3- phosphate [H]

COG id: COG0159

COG function: function code E; Tryptophan synthase alpha chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trpA family [H]

Homologues:

Organism=Escherichia coli, GI1787514, Length=241, Percent_Identity=39.8340248962656, Blast_Score=171, Evalue=6e-44,
Organism=Saccharomyces cerevisiae, GI6321412, Length=266, Percent_Identity=37.9699248120301, Blast_Score=184, Evalue=9e-48,

Paralogues:

None

Copy number: 280 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011060
- InterPro:   IPR018204
- InterPro:   IPR002028 [H]

Pfam domain/function: PF00290 Trp_syntA [H]

EC number: =4.2.1.20 [H]

Molecular weight: Translated: 30880; Mature: 30880

Theoretical pI: Translated: 7.95; Mature: 7.95

Prosite motif: PS00167 TRP_SYNTHASE_ALPHA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDNKMQITKNESLSKVDEKFYELKKNKKIALMPFIMAGDPNIEITSEILLKLQENGADL
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHHHHHHHCCCCCE
IELGIPYSDPLADGPVIQVAASRALKSGTTPRKVITILESLKGKLNIPIILFSYLNPLLC
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
FGFEQFCETASNAGVSGLIIPDLPLEEACKFSKIVSNHSMDLILLVAPTTPFERMKQISN
HCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHC
HTKGFTYLVSVTGVTGERNKMENRVENLIAKLKEINSNPIAVGFGISTPEHVNKVREWGA
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCC
DGVIIGSAFVKRISSSSERDVVDHVGEFCKDMRLAADQKK
CCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKDNKMQITKNESLSKVDEKFYELKKNKKIALMPFIMAGDPNIEITSEILLKLQENGADL
CCCCCEEEECCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCCEEHHHHHHHHHCCCCCE
IELGIPYSDPLADGPVIQVAASRALKSGTTPRKVITILESLKGKLNIPIILFSYLNPLLC
EEECCCCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCCEEHHHHHHHHHH
FGFEQFCETASNAGVSGLIIPDLPLEEACKFSKIVSNHSMDLILLVAPTTPFERMKQISN
HCHHHHHHHHHCCCCCEEEECCCCHHHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHC
HTKGFTYLVSVTGVTGERNKMENRVENLIAKLKEINSNPIAVGFGISTPEHVNKVREWGA
CCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHCC
DGVIIGSAFVKRISSSSERDVVDHVGEFCKDMRLAADQKK
CCEEEHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12917642 [H]