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The map label for this gene is priA [H]

Identifier: 78778883

GI number: 78778883

Start: 466332

End: 468608

Strand: Direct

Name: priA [H]

Synonym: PMT9312_0498

Alternate gene names: 78778883

Gene position: 466332-468608 (Clockwise)

Preceding gene: 78778880

Following gene: 78778887

Centisome position: 27.28

GC content: 31.8

Gene sequence:

>2277_bases
TTGAAGCCGGCAAGTAATCAGTTATTAAATATCTCTTGTAAATTGGAAATTTTGCTTGATATAGGTAGTAGTAATGATAG
CTTTTACTACTTAGATGGAAATAATCTTGGGGTAGAAGTTGGGGATATAGTAATTGTGAAACTAAGAGGGAGATTATTGA
ATGGGTTAGTGATCTCCAAAGAGAACTTTTCGTCAATGAATAAAGATGAAGCAAATAATACTGCAGGAAGAAGTATAAGA
TATTTGTTTGTTGAAGGTATTTTGCAGAAAAAAATAATTGATGACTGTTGGAGTGAATGGATAGAGTCCCTAGCTTCTTT
TTATATGGTTAGTAATTTAAAAATGTTTAAAACGGCATTTCCGCCAGGCTGGATTGGTAAATATAAGAAAATTCCTCAAG
GTTTAAAAGATCAAATATGGATTGAAACCAAAAAAAAATTTGATATAAAAAAAAATGAATTGACCAATAAAGAATTTTTA
TTAATAGATACTTTGCCCGAAAAAGGTAATTGGCAAAGTGAGTTAATAAAGTCTGGTTTTAATTACACTCTAATTAAGTC
ACTGGTTAGTAGAAATTACCTGGTTAAATCTAAAAGAAAAAAAACAATAAATACTAAATTTAATTTCTTTGTAAATGATC
ATATTGCAACGAAGAAACCAAATCTTACAAATGAGCAAAAAATTGCATTTCAGGAATTTCAAACAATGAAACCTGGAGAT
GCGTTACTCCTATGGGGCGAAACAGGTTCAGGTAAAACAGAAGTTTATATGAGAATTTCTGAATATCAATTACTTAAGAA
AAAAAGTTGTTTGATACTTGCACCAGAAATCGGACTTATTCCTCAACTTATTGATAGATTTAGTAAGAGATTTAATAATG
TTGTTTGCGAATATCATAGTAACTGTTCTTCTAGTCATAGAACTTTAGTTTGGAAGAAAATTATTAGTGCTAATGAACCT
CTAATAGTAATAGGAACGAGGTCCTCAATTTTTCTTCCAATTAAAAATCTAGGATTAATAATAATGGATGAAGAACATGA
TGTTTCATATAAACAAGATAGCCCTATGCCTTGTTATGACGCAAGAGAGATTGCTATTGACATAGTAAAAAGGAATTCTG
CAAAGCTAATTTTGGGGAGTGCAACCCCATCAATGAAGACTTGGAAAAAGTGTATTTTCGAAAAGAATTTTAAGTTAGTA
CGAATGATTCAAAGGATATCAAGCAAAGAGGCGCCTGAAATAAGAATTATCGATATGCGAGATGAATTTAAGAAGGGAAA
TATGAAAATTTTATCCAATGAATTATTACAATTGATTCCCCAACTACGCTTAAAGAATGAGCAGGCAATAATTTTGATCC
CTAGAAGGGGGCATAGTGGGTTCTTGAGTTGTAGAAATTGCGGATATTTAGTTAATTGTCCAAACTGTGACGTTCCTTTA
TCGGTACATCTCGGTTCACAAGGAAAAAAATGGTTAAGCTGTCATTGGTGTGATCATAAATCGAGACTGATCAATCGTTG
CCCAGATTGTAATTCAAATGCCTTCAAACCTTTTGGGATAGGGACACAAAGAGTTATAGAGTTTTTAAATGCAGAATTTC
CTGACTTAAGAGTACTTCGCTTTGATCGAGATACAACCTCAGGAAAGGATGGTCATAGAGATATTCTTTCAAAGTTTTCT
AGAGGTGATGCTGATATTCTTGTTGGGACTCAAATGTTAGCAAAAGGGATTGATATCCCTAATATTACTCTTTCAGTAGT
TATTGCAGCAGATGGATTGCTTCATCGTCCAGATATTTCGGCAGAAGAAAAAGCATTACAATTGTTTTTACAATTAGCTG
GCAGGGCAGGCAGGGCTCAAAAAAAAGGAAAAGTTATTTTTCAAACATATAAACCTAACCATCCTGTAATTTCATATCTT
CAAAAAAGAGATTATGAAAGATTTTTAATTGAAAATTCGAACTTGAGAAAAGATGCTAATTTATTTCCATTTTGCAAAAT
TTGCCTTCTTAAATTATCAGGGGAAAACTATGAATTAACTGAATCTATTGCAATAAAATTAGCAAAATATCTACTGAATT
TTTGTGAGAAAAATAACTGGAAATTAATTGGCCCTGCTCCTAGTTTAATTGCTAAAGTCGGGAAAAAATTTAGATGGCAG
ATATTAATACATGGGCCTGAAGGCTCAAAAATACCTTTGCCTGAAAGATCATTATTATGGAAACTTATTCCAAAAAATGT
TTTTTTAATAATTGATGTTAATCCAGCAGAGTTGTAA

Upstream 100 bases:

>100_bases
TCAATGATCAAAGCTTCATGTCTCCCTTAAGAGCAGGATACTTACAATGTGCAAAAAACACTTTGTGGAATGTTCAACAA
TCCAATACTAAGAAAAAGTT

Downstream 100 bases:

>100_bases
TTATTTTGATGGAAGTTGAGGTAAATCTGTACCTAATTTAAATCTATAGAATCTTAATGAATAAGCTAATATTATAATTA
TTAAAATAATAGATATCCCA

Product: primosomal protein N' (replication factor Y)

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 758; Mature: 758

Protein sequence:

>758_residues
MKPASNQLLNISCKLEILLDIGSSNDSFYYLDGNNLGVEVGDIVIVKLRGRLLNGLVISKENFSSMNKDEANNTAGRSIR
YLFVEGILQKKIIDDCWSEWIESLASFYMVSNLKMFKTAFPPGWIGKYKKIPQGLKDQIWIETKKKFDIKKNELTNKEFL
LIDTLPEKGNWQSELIKSGFNYTLIKSLVSRNYLVKSKRKKTINTKFNFFVNDHIATKKPNLTNEQKIAFQEFQTMKPGD
ALLLWGETGSGKTEVYMRISEYQLLKKKSCLILAPEIGLIPQLIDRFSKRFNNVVCEYHSNCSSSHRTLVWKKIISANEP
LIVIGTRSSIFLPIKNLGLIIMDEEHDVSYKQDSPMPCYDAREIAIDIVKRNSAKLILGSATPSMKTWKKCIFEKNFKLV
RMIQRISSKEAPEIRIIDMRDEFKKGNMKILSNELLQLIPQLRLKNEQAIILIPRRGHSGFLSCRNCGYLVNCPNCDVPL
SVHLGSQGKKWLSCHWCDHKSRLINRCPDCNSNAFKPFGIGTQRVIEFLNAEFPDLRVLRFDRDTTSGKDGHRDILSKFS
RGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDISAEEKALQLFLQLAGRAGRAQKKGKVIFQTYKPNHPVISYL
QKRDYERFLIENSNLRKDANLFPFCKICLLKLSGENYELTESIAIKLAKYLLNFCEKNNWKLIGPAPSLIAKVGKKFRWQ
ILIHGPEGSKIPLPERSLLWKLIPKNVFLIIDVNPAEL

Sequences:

>Translated_758_residues
MKPASNQLLNISCKLEILLDIGSSNDSFYYLDGNNLGVEVGDIVIVKLRGRLLNGLVISKENFSSMNKDEANNTAGRSIR
YLFVEGILQKKIIDDCWSEWIESLASFYMVSNLKMFKTAFPPGWIGKYKKIPQGLKDQIWIETKKKFDIKKNELTNKEFL
LIDTLPEKGNWQSELIKSGFNYTLIKSLVSRNYLVKSKRKKTINTKFNFFVNDHIATKKPNLTNEQKIAFQEFQTMKPGD
ALLLWGETGSGKTEVYMRISEYQLLKKKSCLILAPEIGLIPQLIDRFSKRFNNVVCEYHSNCSSSHRTLVWKKIISANEP
LIVIGTRSSIFLPIKNLGLIIMDEEHDVSYKQDSPMPCYDAREIAIDIVKRNSAKLILGSATPSMKTWKKCIFEKNFKLV
RMIQRISSKEAPEIRIIDMRDEFKKGNMKILSNELLQLIPQLRLKNEQAIILIPRRGHSGFLSCRNCGYLVNCPNCDVPL
SVHLGSQGKKWLSCHWCDHKSRLINRCPDCNSNAFKPFGIGTQRVIEFLNAEFPDLRVLRFDRDTTSGKDGHRDILSKFS
RGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDISAEEKALQLFLQLAGRAGRAQKKGKVIFQTYKPNHPVISYL
QKRDYERFLIENSNLRKDANLFPFCKICLLKLSGENYELTESIAIKLAKYLLNFCEKNNWKLIGPAPSLIAKVGKKFRWQ
ILIHGPEGSKIPLPERSLLWKLIPKNVFLIIDVNPAEL
>Mature_758_residues
MKPASNQLLNISCKLEILLDIGSSNDSFYYLDGNNLGVEVGDIVIVKLRGRLLNGLVISKENFSSMNKDEANNTAGRSIR
YLFVEGILQKKIIDDCWSEWIESLASFYMVSNLKMFKTAFPPGWIGKYKKIPQGLKDQIWIETKKKFDIKKNELTNKEFL
LIDTLPEKGNWQSELIKSGFNYTLIKSLVSRNYLVKSKRKKTINTKFNFFVNDHIATKKPNLTNEQKIAFQEFQTMKPGD
ALLLWGETGSGKTEVYMRISEYQLLKKKSCLILAPEIGLIPQLIDRFSKRFNNVVCEYHSNCSSSHRTLVWKKIISANEP
LIVIGTRSSIFLPIKNLGLIIMDEEHDVSYKQDSPMPCYDAREIAIDIVKRNSAKLILGSATPSMKTWKKCIFEKNFKLV
RMIQRISSKEAPEIRIIDMRDEFKKGNMKILSNELLQLIPQLRLKNEQAIILIPRRGHSGFLSCRNCGYLVNCPNCDVPL
SVHLGSQGKKWLSCHWCDHKSRLINRCPDCNSNAFKPFGIGTQRVIEFLNAEFPDLRVLRFDRDTTSGKDGHRDILSKFS
RGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDISAEEKALQLFLQLAGRAGRAQKKGKVIFQTYKPNHPVISYL
QKRDYERFLIENSNLRKDANLFPFCKICLLKLSGENYELTESIAIKLAKYLLNFCEKNNWKLIGPAPSLIAKVGKKFRWQ
ILIHGPEGSKIPLPERSLLWKLIPKNVFLIIDVNPAEL

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=631, Percent_Identity=33.2805071315372, Blast_Score=336, Evalue=3e-93,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 86553; Mature: 86553

Theoretical pI: Translated: 10.00; Mature: 10.00

Prosite motif: PS00675 SIGMA54_INTERACT_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPASNQLLNISCKLEILLDIGSSNDSFYYLDGNNLGVEVGDIVIVKLRGRLLNGLVISK
CCCCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCCCEEECCEEEEEECCHHHCCEEEEC
ENFSSMNKDEANNTAGRSIRYLFVEGILQKKIIDDCWSEWIESLASFYMVSNLKMFKTAF
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
PPGWIGKYKKIPQGLKDQIWIETKKKFDIKKNELTNKEFLLIDTLPEKGNWQSELIKSGF
CCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCCCEEEEEECCCCCCCCHHHHHHCCC
NYTLIKSLVSRNYLVKSKRKKTINTKFNFFVNDHIATKKPNLTNEQKIAFQEFQTMKPGD
CHHHHHHHHHCCHHHHCCCCCEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
ALLLWGETGSGKTEVYMRISEYQLLKKKSCLILAPEIGLIPQLIDRFSKRFNNVVCEYHS
EEEEEECCCCCCEEEEEEEHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEEHHCC
NCSSSHRTLVWKKIISANEPLIVIGTRSSIFLPIKNLGLIIMDEEHDVSYKQDSPMPCYD
CCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCC
AREIAIDIVKRNSAKLILGSATPSMKTWKKCIFEKNFKLVRMIQRISSKEAPEIRIIDMR
CHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECH
DEFKKGNMKILSNELLQLIPQLRLKNEQAIILIPRRGHSGFLSCRNCGYLVNCPNCDVPL
HHCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCEEEECCCCCCEE
SVHLGSQGKKWLSCHWCDHKSRLINRCPDCNSNAFKPFGIGTQRVIEFLNAEFPDLRVLR
EEEECCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEE
FDRDTTSGKDGHRDILSKFSRGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDIS
ECCCCCCCCCCHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCEEEEEEECCCCEECCCCC
AEEKALQLFLQLAGRAGRAQKKGKVIFQTYKPNHPVISYLQKRDYERFLIENSNLRKDAN
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCC
LFPFCKICLLKLSGENYELTESIAIKLAKYLLNFCEKNNWKLIGPAPSLIAKVGKKFRWQ
CCHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEE
ILIHGPEGSKIPLPERSLLWKLIPKNVFLIIDVNPAEL
EEEECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC
>Mature Secondary Structure
MKPASNQLLNISCKLEILLDIGSSNDSFYYLDGNNLGVEVGDIVIVKLRGRLLNGLVISK
CCCCCCCEEEEEEEEEEEEEECCCCCCEEEECCCCCCEEECCEEEEEECCHHHCCEEEEC
ENFSSMNKDEANNTAGRSIRYLFVEGILQKKIIDDCWSEWIESLASFYMVSNLKMFKTAF
CCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHCC
PPGWIGKYKKIPQGLKDQIWIETKKKFDIKKNELTNKEFLLIDTLPEKGNWQSELIKSGF
CCCHHHHHHHCCCCCCCCEEEEECCCCCCCHHCCCCCEEEEEECCCCCCCCHHHHHHCCC
NYTLIKSLVSRNYLVKSKRKKTINTKFNFFVNDHIATKKPNLTNEQKIAFQEFQTMKPGD
CHHHHHHHHHCCHHHHCCCCCEECCEEEEEEECCCCCCCCCCCCCHHHHHHHHHCCCCCC
ALLLWGETGSGKTEVYMRISEYQLLKKKSCLILAPEIGLIPQLIDRFSKRFNNVVCEYHS
EEEEEECCCCCCEEEEEEEHHHHHHHCCCCEEECCCCCHHHHHHHHHHHHHHHEEEHHCC
NCSSSHRTLVWKKIISANEPLIVIGTRSSIFLPIKNLGLIIMDEEHDVSYKQDSPMPCYD
CCCCCCHHHHHHHHHCCCCCEEEEECCCEEEEECCCCCEEEEECCCCCCCCCCCCCCCCC
AREIAIDIVKRNSAKLILGSATPSMKTWKKCIFEKNFKLVRMIQRISSKEAPEIRIIDMR
CHHHEEEEEECCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEEEECH
DEFKKGNMKILSNELLQLIPQLRLKNEQAIILIPRRGHSGFLSCRNCGYLVNCPNCDVPL
HHCCCCCEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCEEECCCCEEEECCCCCCEE
SVHLGSQGKKWLSCHWCDHKSRLINRCPDCNSNAFKPFGIGTQRVIEFLNAEFPDLRVLR
EEEECCCCCCEEEEEECCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCEEEEE
FDRDTTSGKDGHRDILSKFSRGDADILVGTQMLAKGIDIPNITLSVVIAADGLLHRPDIS
ECCCCCCCCCCHHHHHHHHCCCCCEEEECHHHHHCCCCCCCCEEEEEEECCCCEECCCCC
AEEKALQLFLQLAGRAGRAQKKGKVIFQTYKPNHPVISYLQKRDYERFLIENSNLRKDAN
HHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCHHHHHHHHCCCCEEEEECCCCCCCCC
LFPFCKICLLKLSGENYELTESIAIKLAKYLLNFCEKNNWKLIGPAPSLIAKVGKKFRWQ
CCHHHHHEEEEECCCCEEHHHHHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHCCCEEEE
ILIHGPEGSKIPLPERSLLWKLIPKNVFLIIDVNPAEL
EEEECCCCCCCCCCCHHHHHHCCCCCEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]