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The map label for this gene is spoT [H]

Identifier: 78778579

GI number: 78778579

Start: 183491

End: 185800

Strand: Reverse

Name: spoT [H]

Synonym: PMT9312_0193

Alternate gene names: 78778579

Gene position: 185800-183491 (Counterclockwise)

Preceding gene: 78778581

Following gene: 78778577

Centisome position: 10.87

GC content: 34.11

Gene sequence:

>2310_bases
ATGTCTGAGGCAGCTGCAAATTCAAAAGAAAAAAACGAAATTGAAGTTTCCAAAACCATTTTGCCTGAAAATAAAAAATA
TGAAAGTGAATCTTTGAATTATCAAATAAAAATTCCCGATTGGCTTCTTAAAGATATTCATAATTTTGAAAAATCAAATA
AAGAAAATGATGAGAATCAAAACCTTTTAGTAAAGGCTTTTAAACTTGCTTATAAAGCTCATGATGGACAATTCCGCGCG
AGTGGCGAGCCATACATTATCCACCCGGTTGCTGTTGCAAATCTCCTCAAAGAAATAGGTGCTAGTACATCTGTTATTGC
TGCAGGCCTTTTACATGATGTTGTTGAAGATACTGGCATTGATTTATCCGAAATAGAAACAAATTTTGGATTAGAAGTAA
AAATACTTGTAGAAGGTGTAACAAAATTAGGAGGCATTCACTTTAACAACAGGACTGAAGCACAAGCTGAAAATCTTAGG
AAAATGTTTTTGGCTATGGCCAGCGATATCAGAGTTGTTTTAGTAAAACTTGCAGATCGACTTCATAACATGAGAACAAT
TGAATGGCTAAATGATGAGAAAAAACTAAGAATAGCAAGAGAAACAAGAGAGATTTATGCACCATTAGCTAATCGACTAG
GAATAAACAGATTTAAATGGGAATTAGAAGATTTAGCTTTTAAATTCCTAGAGCCTAAAGAATATAAAGATCTTAAAGAT
CAAATCGCTGTTAAAAGAAGTGATAGAGAAAAAAGATTAAAAGTAACTTTGAATCTTATGAAGGAAAACTTGGTTTCAGC
AGGTTTGAAAAATTTTGAAATAACAGGCAGGCCAAAACATCTTTATGGCATCTGGAGCAAAATGGAAAGACAACAAAAGC
ATTTTCACGAGATTTATGATGTTGCTGCCCTAAGAATTATCGTGGACAATTCTGATAGTTGTTATAGAGCTTTAGCAGTG
GTTCATGATACTTTCAAACCAATTCCAGGTAGATTTAAAGACTATATAGGATTACCAAAACCCAATGGATACCAGTCCTT
ACACACTTCTGTGATTGGAAGACATCGACCTATTGAAGTTCAAATTAGAACTACTTCCATGCATCAAATTGCTGAATATG
GTATTGCCGCTCATTGGCAATACAAAGAGGGTGGTTCTCCTGCTAAAAGTAATGCCGAGAGATTTAATTGGCTAAGACAA
TTAGTAGAATGGCAACAAGAAGGTAATGAAGGGGATCATAATGATTATTTAGCTTCAATTAAAGAAGATTTATTTGATGA
AGAAGTATTTGTGATCACTCCAAAAGGAGATGTTGTTGGTTTAAGGAAAGGATCTACCGCGATAGATTTCGCCTACAGAA
TTCATTCTGAAGTTGGAAATCACTGTAATGGAATAAGAATTAATGAAAAGCTTTCTCCATTATCTACAGCACTTCAAAAT
GGTGACTTCATAGAAATTTTGACAAGTAATAATGCTACTCCAAGCTTGGATTGGCTGAACTTTGTAGTTACGCCAACTGC
TAAAAATAGAATCCGCCAATGGTATAAGAAAAGCCATCGTGATGAAACGATTAAAAGAGGTAGAGATTTACTTGAAAAAG
AAGTAGGTCGAAACGGTTTTGAAGCATTAATTTCTAGTGAAGCCATGAAAAAAGTTGCAAATCGATGCAATTTAAAAACT
TCTGAAGACCTTCTTGCATCTCTTGGTTTTGGTGGTTTAACTTTGCATCAAGTATTAAACAGACTAAGAGAAGAAATAAA
ATTACAGACAGAAGATGTAAAAAATGATTCTGACTCTGAAATAGCAAAATCTCTTAAAAGTAATAGTAATTTATCCACTA
ATAAATCTAATACGGCAGCTAGATCACCAATTTCCGGGATAGAAGGTCTTGATTACAGAATAGGTAAATGCTGTTCCCCA
CTCCCAGGCGAAGATATAGTCGGCACAGTATCGCTCGGCAACCATGGGATAACCATACATAGGGAAGATTGTGAAAATGT
AATACCAATTCCAATAGAGAGAAGATTACCTGTTGGTTGGAATCAAGATAATAAAACTGGCGATAATAAGTTTCCAATTC
AGCTACGAATAGAAGTAATTGATAGAGTTGGAGTTCTTAAAGATATTCTTATGCGGTTATCTGATAAAGGTATAAACGTT
AGCGATGCCAATGTTAAAACTGCTTATGGTAAACCAGCTATTATAAATCTTTGTGTAGGTCTTGAAAGTTTTAATCAACT
TCACAAAACAATTGAACAAATTAAATCAATGGCAGATGTTTTAGATATTGCCAGAGTTGGACAAAGTTAA

Upstream 100 bases:

>100_bases
CAGTAAGATTTTTAACTAAAATAATTTTTTCTTTATTTATTTCCATGATTTATAGCAAATTTTTCTAGACCTAAATAAAA
TACATCTAAAGCAATATAAA

Downstream 100 bases:

>100_bases
TTTTAAAATCAGATCTATCTATCTTTTACTTTTTATCTTGTAGATAAAGAAAACAATACTGCCGCAAATCAAAGCTAATA
AAATACCTACACAAGATGAA

Product: guanosine-3',5'-bis(diphosphate) 3'-diphosphatase, (ppGpp)ase

Products: NA

Alternate protein names: Penta-phosphate guanosine-3'-pyrophosphohydrolase; (ppGpp)ase [H]

Number of amino acids: Translated: 769; Mature: 768

Protein sequence:

>769_residues
MSEAAANSKEKNEIEVSKTILPENKKYESESLNYQIKIPDWLLKDIHNFEKSNKENDENQNLLVKAFKLAYKAHDGQFRA
SGEPYIIHPVAVANLLKEIGASTSVIAAGLLHDVVEDTGIDLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLR
KMFLAMASDIRVVLVKLADRLHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYKDLKD
QIAVKRSDREKRLKVTLNLMKENLVSAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYDVAALRIIVDNSDSCYRALAV
VHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEVQIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQ
LVEWQQEGNEGDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGFEALISSEAMKKVANRCNLKT
SEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSEIAKSLKSNSNLSTNKSNTAARSPISGIEGLDYRIGKCCSP
LPGEDIVGTVSLGNHGITIHREDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
SDANVKTAYGKPAIINLCVGLESFNQLHKTIEQIKSMADVLDIARVGQS

Sequences:

>Translated_769_residues
MSEAAANSKEKNEIEVSKTILPENKKYESESLNYQIKIPDWLLKDIHNFEKSNKENDENQNLLVKAFKLAYKAHDGQFRA
SGEPYIIHPVAVANLLKEIGASTSVIAAGLLHDVVEDTGIDLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLR
KMFLAMASDIRVVLVKLADRLHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYKDLKD
QIAVKRSDREKRLKVTLNLMKENLVSAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYDVAALRIIVDNSDSCYRALAV
VHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEVQIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQ
LVEWQQEGNEGDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGFEALISSEAMKKVANRCNLKT
SEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSEIAKSLKSNSNLSTNKSNTAARSPISGIEGLDYRIGKCCSP
LPGEDIVGTVSLGNHGITIHREDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
SDANVKTAYGKPAIINLCVGLESFNQLHKTIEQIKSMADVLDIARVGQS
>Mature_768_residues
SEAAANSKEKNEIEVSKTILPENKKYESESLNYQIKIPDWLLKDIHNFEKSNKENDENQNLLVKAFKLAYKAHDGQFRAS
GEPYIIHPVAVANLLKEIGASTSVIAAGLLHDVVEDTGIDLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLRK
MFLAMASDIRVVLVKLADRLHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYKDLKDQ
IAVKRSDREKRLKVTLNLMKENLVSAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYDVAALRIIVDNSDSCYRALAVV
HDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEVQIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQL
VEWQQEGNEGDHNDYLASIKEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQNG
DFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGFEALISSEAMKKVANRCNLKTS
EDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSEIAKSLKSNSNLSTNKSNTAARSPISGIEGLDYRIGKCCSPL
PGEDIVGTVSLGNHGITIHREDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINVS
DANVKTAYGKPAIINLCVGLESFNQLHKTIEQIKSMADVLDIARVGQS

Specific function: In eubacteria ppGpp (guanosine 3'-diphosphate 5-' diphosphate) is a mediator of the stringent response that coordinates a variety of cellular activities in response to changes in nutritional abundance. This enzyme catalyzes the degradation of ppGpp into G

COG id: COG0317

COG function: function code TK; Guanosine polyphosphate pyrophosphohydrolases/synthetases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HD domain [H]

Homologues:

Organism=Homo sapiens, GI38348360, Length=139, Percent_Identity=37.410071942446, Blast_Score=72, Evalue=3e-12,
Organism=Escherichia coli, GI1790082, Length=711, Percent_Identity=39.943741209564, Blast_Score=527, Evalue=1e-150,
Organism=Escherichia coli, GI1789147, Length=698, Percent_Identity=34.9570200573066, Blast_Score=450, Evalue=1e-127,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR012675
- InterPro:   IPR003607
- InterPro:   IPR007685
- InterPro:   IPR004811
- InterPro:   IPR004095
- InterPro:   IPR012676 [H]

Pfam domain/function: PF01842 ACT; PF04607 RelA_SpoT; PF02824 TGS [H]

EC number: =3.1.7.2 [H]

Molecular weight: Translated: 86949; Mature: 86818

Theoretical pI: Translated: 7.92; Mature: 7.92

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEAAANSKEKNEIEVSKTILPENKKYESESLNYQIKIPDWLLKDIHNFEKSNKENDENQ
CCCCCCCCCCCCCEEHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCH
NLLVKAFKLAYKAHDGQFRASGEPYIIHPVAVANLLKEIGASTSVIAAGLLHDVVEDTGI
HHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLRKMFLAMASDIRVVLVKLADR
CHHHHHCCCCCEEEEHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYKDLKD
HHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHH
QIAVKRSDREKRLKVTLNLMKENLVSAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYD
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
VAALRIIVDNSDSCYRALAVVHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEV
HHEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
QIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQLVEWQQEGNEGDHNDYLASI
EEECCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
HHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHCC
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGF
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHH
EALISSEAMKKVANRCNLKTSEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCHHH
IAKSLKSNSNLSTNKSNTAARSPISGIEGLDYRIGKCCSPLPGEDIVGTVSLGNHGITIH
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEE
REDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
SDANVKTAYGKPAIINLCVGLESFNQLHKTIEQIKSMADVLDIARVGQS
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SEAAANSKEKNEIEVSKTILPENKKYESESLNYQIKIPDWLLKDIHNFEKSNKENDENQ
CCCCCCCCCCCCEEHHHHCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHCCCCCCCCH
NLLVKAFKLAYKAHDGQFRASGEPYIIHPVAVANLLKEIGASTSVIAAGLLHDVVEDTGI
HHHHHHHHHHHHHCCCCEECCCCCEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
DLSEIETNFGLEVKILVEGVTKLGGIHFNNRTEAQAENLRKMFLAMASDIRVVLVKLADR
CHHHHHCCCCCEEEEHHHHHHHHCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LHNMRTIEWLNDEKKLRIARETREIYAPLANRLGINRFKWELEDLAFKFLEPKEYKDLKD
HHCCEEEECCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCHHHHHHHH
QIAVKRSDREKRLKVTLNLMKENLVSAGLKNFEITGRPKHLYGIWSKMERQQKHFHEIYD
HHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHH
VAALRIIVDNSDSCYRALAVVHDTFKPIPGRFKDYIGLPKPNGYQSLHTSVIGRHRPIEV
HHEEEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCHHHHHHHHHCCCCCEEE
QIRTTSMHQIAEYGIAAHWQYKEGGSPAKSNAERFNWLRQLVEWQQEGNEGDHNDYLASI
EEECCHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHH
KEDLFDEEVFVITPKGDVVGLRKGSTAIDFAYRIHSEVGNHCNGIRINEKLSPLSTALQN
HHHHCCCCEEEEECCCCEEEECCCCCHHHHHHHHHHHHCCCCCCEEECCCCCHHHHHHCC
GDFIEILTSNNATPSLDWLNFVVTPTAKNRIRQWYKKSHRDETIKRGRDLLEKEVGRNGF
CCEEEEEECCCCCCCCCCEEEEECCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCHH
EALISSEAMKKVANRCNLKTSEDLLASLGFGGLTLHQVLNRLREEIKLQTEDVKNDSDSE
HHHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHCCCCCHHH
IAKSLKSNSNLSTNKSNTAARSPISGIEGLDYRIGKCCSPLPGEDIVGTVSLGNHGITIH
HHHHHHCCCCCCCCCCCCHHCCCCCCCCCCCHHHHHCCCCCCCCCCEEEEEECCCCEEEE
REDCENVIPIPIERRLPVGWNQDNKTGDNKFPIQLRIEVIDRVGVLKDILMRLSDKGINV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHHCCCCCCC
SDANVKTAYGKPAIINLCVGLESFNQLHKTIEQIKSMADVLDIARVGQS
CCCCCEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8905231 [H]